A Wide Extent of Inter-Strain Diversity in Virulent and Vaccine Strains of Alphaherpesviruses
Figure 8
Percent of data supporting polymorphic base calls in PRV genomes.
A) A limited number of polymorphic bases were detected in the plaque-purified strains Kaplan, Becker, and Bartha; these were analyzed to deduce the percent of sequence data supporting the primary vs. alternative base calls. The majority of polymorphic sites show 1–20% support for the alternative base call, or 99–80% support for the primary base call. B) After passaging the purified Becker stock multiple times in vitro (Becker p10), there was no increase in the overall number of polymorphic bases, and only a slight shift in the degree of support for alternative base calls. C) However in an unpurified historical stock of PRV Kaplan, which is the parent of the plaque-purified stock used for sequencing, hundreds of polymorphic bases were observed. Despite the larger quantity of polymorphic bases, the degree of support for the alternative base is similar to that found in the plaque-purified strains. D) Graph displays the alternative base calls for the four most variant polymorphisms in PRV Becker (41–50% bin), and how these specific bases were called in the Becker p10 progeny stock (the height of each base letter corresponds to its frequency). These sites display shifts in base frequency in the Becker p10 stock.