Beta-HPV 5 and 8 E6 Promote p300 Degradation by Blocking AKT/p300 Association
Figure 1
E6 from α and β HPVs bind p300 with different strengths.
(A) Representative immunoblot showing the levels of p300 co-precipitating with various GST-tagged E6 proteins. Equal levels of input GST-E6 are demonstrated by the GST immunoblot in the lower portion of the figure. (B) HFKs expressing E6-HA were immunoprecipitated with a HA antibody (HA) or mouse Immunoglobulin G (IgG) as a negative control and immunoblotted with a p300 and HA antibody. (C) Identical cell lysates were immunoprecipitated with a p300 antibody (p300) or IgG control and immunoblotted with a p300 and HA antibody. Input is equal to 5% of total protein lysate.