In vivo Hypoxia and a Fungal Alcohol Dehydrogenase Influence the Pathogenesis of Invasive Pulmonary Aspergillosis
Figure 8
Representative histopathology of the chemotherapy and triamcinolone mouse models 3 and 4 days after inoculation.
Mice were inoculated with 1x104 conidia by inhalation (Control = 0.01% Tween inoculated), euthanized on indicated days, lungs removed, fixed, and stained with hematoxylin and eosin (H&E) or Gommori's methenamine silver (GMS) stain. (A) In the chemotherapy IPA model wild type inoculated mice show substantial fungal growth and a strongly reduced influx of immune cells, while lesions of ΔalcC inoculated mice show reduced fungal growth and an increased inflammatory response on day 3 and day 4 p.i.. (B) Lung histopathology of triamcinolone model mice showed reduced fungal growth of the wild type with robust inflammation compared to (A) and an even further reduction in growth of ΔalcC with an apparent increase in inflammation and cellular influx. Bar = 100 µm.