Re-localization of Cellular Protein SRp20 during Poliovirus Infection: Bridging a Viral IRES to the Host Cell Translation Apparatus
Figure 8
SRp20 and PCBP2 co-sedimentation with 40S ribosomal subunits in poliovirus-infected cells (5 hr post-infection).
Extracts were generated from poliovirus-infected HeLa cells after 5 hours of infection and processed as described in the legend for Figure 6. Representative polysome profiles and Western blot analyses are shown. The sedimentation of virus (labeled ‘virus peak’ in the profile) was determined by Western blot analysis of fractions using an anti-VP2 polyclonal antibody that detects the poliovirus VP2 capsid protein (Holzberg, Nguyen, and Semler, unpublished).