Kupffer Cells Hasten Resolution of Liver Immunopathology in Mouse Models of Viral Hepatitis
Figure 5
Impaired removal of apoptotic hepatocytes and focal hepatocellular necrosis in Clo-L-treated mice.
(A,C) Representative immunohistochemical micrographs of control (left panels) or Clo-L-treated (right panels) HBV replication-competent transgenic livers, one (A) or five (C) days after intravenous injection of 107 HBV-specific CTL. Cleaved caspase 3 (CC3) staining in brown. The number (mean ± standard deviation) of CC3+ hepatocytes was calculated in 100 high power fields (corresponding to about 4 mm2 of liver tissue) and is indicated in bottom right insets. N.D.: non detectable. Scale bars represent 150 µm or, in the case of the upper left inset, 20 µm. n = 6, p<0.05. (B) Representative micrographs of hematoxylin/eosin-stained control (left) or Clo-L-treated (right) HBV replication-competent transgenic livers, two days after intravenous injection of 107 HBV-specific CTL. Broken line delineates necroinflammatory foci. Data are representative of at least 3 independent experiments that gave similar results.