A Large and Intact Viral Particle Penetrates the Endoplasmic Reticulum Membrane to Reach the Cytosol
Figure 3
Immunoprecipitation of ER- and cytosol-localized SV40 using conformation-specific antibodies.
(A) Cells were infected with SV40 for 12 hrs, harvested, and processed according to Figure 1C to obtain S1 and S2. These fractions were incubated with the indicated VP1 antibody concentration, or a control antibody. The precipitated samples were subjected to SDS-PAGE followed by immunoblotting against VP1. 5% input is shown. (B) S2 in A and WT SV40 were subjected to immunoprecipitation using either VP1 or a control antibody, and the precipitated sample subjected to SDS-PAGE and immunoblotted with antibodies against VP2/VP3 or VP1. 5% input is shown. (C) The inputs and immunoprecipitates in B were subjected to PCR to amplify a region of SV40 genome. 5% input is shown. (D) As in B, except the S1 was used for immunoprecipitation. (E) As in C, except the S1 was used as the starting material.