A Novel Toxoplasma gondii Nuclear Factor TgNF3 Is a Dynamic Chromatin-Associated Component, Modulator of Nucleolar Architecture and Parasite Virulence
Figure 1
Affinity purification of T. gondii nuclear factors.
A). A schematic diagram showing the experimental strategy devised for the purification of ENO1 promoter binding proteins. B) Electrophoretic mobility shift assays (EMSA) showing DNA-protein complexes using the biotinylated DNA sequence corresponding to the ENO1 promoter and total nuclear extract of tachyzoites. Lane 1, unbound biotinylated probe alone. Lane 2, gel shift binding assays revealing the biotinylated DNA-protein complexes. Lane 3, specific competitor corresponding to unlabeled ENO1 promoter introduced simultaneously with labelled probe during binding assays. C) SDS-PAGE and silver staining after a large scale affinity purification of ENO1 probe bound proteins using a total nuclear extract from T. gondii tachyzoites. The markers (MW) indicated in kilodaltons (kDa) are shown on the left.