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Host Iron Withholding Demands Siderophore Utilization for Candida glabrata to Survive Macrophage Killing

Figure 1

Heme is an inefficient source of Fe for C. Glabrata.

A Hemolytic activity of Candida albicans, C. glabrata and S. cerevisiae. Wild type strains were streaked onto 5% blood sheep agar plates and grown at 30 °C or 37 °C with 5% CO2 for 48–72 hours. B Heme does not efficiently promote the growth of C. glabrata under Fe deficiency. Candida albicans, C. glabrata and S. cerevisiae were grown in Fe-deficient medium supplemented with the indicated concentrations of hemin and growth determined after 12 hours. C Candida albicans and C. glabrata were grown under Fe deficiency supplemented or not with 20 µM Zn-PPIX for 3 hours and observed by live fluorescence microscopy.

Figure 1