The ESCRT-0 Component HRS is Required for HIV-1 Vpu-Mediated BST-2/Tetherin Down-Regulation
Figure 6
Effect of HRS depletion on the release of HIV-1 particles from non-restrictive HEK 293T cells.
HeLa cells and HEK 293T cells transfected with either control siRNA (CT) or siRNA targeting HRS were infected with VSV-G-pseudotyped NL4-3 HIV-1 (NL4-3 WT). (A) Western-blot analysis of infected siRNA-treated cells (Upper panels) and viral particles released in the supernatant (Lower panel). Tubulin is the loading control. (B, C) CAp24 present within the cells (Cell-associated CAp24, dark grey graph bars) and released from the infected cells (Released CAp24, light-grey graph bars) was measured by ELISA quantification. The experiment was performed in duplicate. For each cell type, values obtained for HRS depleted cells were normalized to those obtained for the control cells (set as 100%). Bars represent the mean −/+ SD from 3 independent experiments.