Selective C-Rel Activation via Malt1 Controls Anti-Fungal TH-17 Immunity by Dectin-1 and Dectin-2
Figure 3
Malt1 paracaspase activity is required for c-Rel activation and cytokine induction by dectin-1.
(A) DNA binding of NF-κB subunits in nuclear extracts of curdlan-stimulated DCs after inhibition of Malt1 paracaspase activity by z-VRPR-FMK. Data are representative of three independent experiments. (B) Quantitative real-time PCR for indicated mRNAs in curdlan-stimulated DCs after Malt1 paracaspase inhibition. Expression is normalized to GAPDH and set at 1 in curdlan-stimulated cells. Data are mean ± s.d. of three independent experiments, **p<0.01 (Student's t-test). (C) Cytokine production was determined by ELISA in supernatants of DCs stimulated with curdlan in the absence or presence of Malt1 paracaspase inhibitor. Data are mean ± s.d. of duplicate cultures, and are representative of five independent experiments, *p<0.05 and **p<0.01 (Student's t-test).