Rapid End-Point Quantitation of Prion Seeding Activity with Sensitivity Comparable to Bioassays
Figure 6
RT-QuIC end-point dilution analysis of CSF from scrapie-infected hamsters.
CSF was collected at the clinical stage of disease from two individual hamsters (Panel A and B) i.c.-inoculated with 263K scrapie BH. CSF was serially diluted 5-fold and analyzed by RT-QuIC. Each 0.75 h time point for each dilution is represented as an average ThT fluorescence from 8 replicate wells. The average Spearman-Kärber estimates of the SD50/2 µl (the seed volume) and SD50/ml neat CSF are shown.