Immune Evasion by Yersinia enterocolitica: Differential Targeting of Dendritic Cell Subpopulations In Vivo
Figure 4
Injection of Yops into DCs upon infection of mice with Ye.
(A & B) C57BL/6 mice were injected i.v. with 5×105 Ye E40-YopE53β-lactamase (YopE-βla), 5×105 Ye E40-YopE53-OVA (YopE-OVA), or PBS. 24 h p.i. the percentage of DC subpopulations in the spleen injected with YopE-βla was analyzed by flow cytometry using CCF4 substrate emitting a blue fluorescence when degraded by β-lactamase in the cytosol. (A) The dot plots show the percentage ± standard deviation of CCF4-blue+CD11chi cells from mice treated with PBS, infected with the negative control Ye YopE-OVA mutant strain, or infected with the Ye YopE-βla mutant strain. R1 indicates the blue cells (Yop injected) and R2 the green cells (not injected). The diagram shows the percentage of blue+ DC subpopulations of mice infected with the Ye YopE-βla mutant strain. Data shown are the summary of 2 independent experiments with 5–6 mice infected with YopE-βla mutant strain in each experiment. (B) The expression of MHC class II and CD86 on DC subpopulations from mice injected with PBS or infected with YopE-βla mutant strain was analyzed as described in (A) (R1: blue cells, R2: green cells). The diagrams show the MHC class II or CD86 expression of the DC subpopulations as mean fluorescence and are representative for 2 independent experiments with 5–6 mice infected with YopE-βla mutant strain in each experiment. * indicate statistically significant differences.