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Activation of Akt Signaling Reduces the Prevalence and Intensity of Malaria Parasite Infection and Lifespan in Anopheles stephensi Mosquitoes

Figure 2

Expression profile of the transgene during a reproductive cycle.

A. Total RNA was isolated from the midguts and carcasses of ten non-bloodfed (NBF) and ten bloodfed transgenic mosquitoes at 2 h, 24 h, 48 h and 72 h post-bloodfeeding and converted into cDNA. The 72 h sample was collected post-oviposition. Transgene specific qRT-PCR primers were used to amplify myr-AsteAkt from the cDNA; amplification of ribosomal protein S7 was used for normalization. The qRT-PCR experiments were performed in triplicate and replicated twice with separate cohorts of mosquitoes. Data are represented as means ± SEMs. B. Total protein was isolated from the midguts of transgenic mosquitoes at various time points during a reproductive cycle. Proteins were blotted and probed with anti-HA antibody or anti-GAPDH antibody to assess protein loading. C. Average expression of transgenic protein normalized to GAPDH loading controls and shown relative to levels in non-bloodfed mosquitoes (NBF). Data are represented as means ± SEMs from four replicates with separate cohorts of mosquitoes.

Figure 2

doi: https://doi.org/10.1371/journal.ppat.1001003.g002