Two Novel Point Mutations in Clinical Staphylococcus aureus Reduce Linezolid Susceptibility and Switch on the Stringent Response to Promote Persistent Infection
The major features of the clinical case and the relevant clinical isolates are demonstrated. Included are photos of overnight cultures on HBA depicting the normal MRSA strain (JKD6210) and the SCV strain (JKD6229) isolated after many weeks of failed antimicrobial therapy. Pulsed field gel electrophoresis results of SmaI digested DNA from the paired clinical isolates are also included (lane 1 JKD6210, lane 2 JKD6229) and demonstrate an identical banding pattern for the two strains. #Note, JKD6229 demonstrated reduced linezolid susceptibility within the susceptible MIC range.