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Deficiencies in Jasmonate-Mediated Plant Defense Reveal Quantitative Variation in Botrytis cinerea Pathogenesis

Figure 6

Directed measurement of camalexin biosynthetic transcript as compared to camalexin accumulation in the same tissues.

A) Relative transcript levels of PAD3 (At3g26830) in leaves of wild-type (filled bars) and coi1 (open bars) A. thaliana leaves at 24 and 48 hours post-inoculation with B. cinerea isolates BcGrape, Bc83-2, or a mock inoculation. Data are means of four independent biological replicates with bars indicating standard error. Within each figure, letters above bars indicate statistical significance; bars not sharing letters represent significant mean differences at p<0.05. B) Relative transcript level of PAD3 at 48 hpi (x-axis) in relation to camalexin accumulated (ng/g leaf tissue). Each point represents a single sample. Filled symbols represent samples from wild-type plants and open symbols represent samples from coi1 plants. Treatments are indicated as: square = mock, triangle = BcGrape, circle = Bc83-2. PAD3 transcript levels were normalized to transcript levels of reference genes At4g26410 and At4g34270 measured in the same samples.

Figure 6