Interaction of Cryptococcus neoformans Rim101 and Protein Kinase A Regulates Capsule
Figure 2
Rim101 retains conserved phenotypes from other fungal species.
A. The rim101Δ mutant is sensitive to alkaline pH. Cells were incubated in buffered YNB, and growth was determined by cell counts after 72 hours. B. The rim101Δ mutant is sensitive to salt stress. 1×105 cells from each strain were serially diluted (5-fold dilution) onto YPD plates containing 1.5M NaCl or 200 mM LiCl. The plates were incubated at 30°C for 3 days. Cells were plated onto YPD plates for 48 hours as a control.