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Fis Is Essential for Capsule Production in Pasteurella multocida and Regulates Expression of Other Important Virulence Factors

Figure 2

Schematic representation of the P. multocida serogroup A capsule biosynthetic locus and identification of the hyaE transcriptional start site.

The organization of the genes in the capsule biosynthetic locus is shown in panel A and the nucleotide sequence of the intergenic region between phyA and hyaE is shown in the flyout box. The positions of the HyaE and PhyA translational initiation codons (ATG) are designated by right-angled arrowheads and the identified transcriptional start site for hyaE is shown in bold text and labeled +1. Predicted RBS, −10 and −35 sites are underlined, and labeled above the sequence. Predicted Fis binding sites based on the E. coli position specific weight matrix are boxed [15]. The length (x-axis) and quantity (y-axis) of hyaE-specific primer extension products generated using VP161 total RNA and the primer BAP5476 are shown in panel B. The exact size (in bp) of the major primer extension products are shown beside the peaks.

Figure 2