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Fis Is Essential for Capsule Production in Pasteurella multocida and Regulates Expression of Other Important Virulence Factors

Figure 1

Hyaluronic acid (HA) capsular polysaccharide produced by P. multocida strains.

The amount of capsular material is shown for paired acapsular (AL1114, AL1162 and AL1396) and capsulated (AL1115, AL1163 and AL1397) variants and the same strains complemented with either empty vector (AL1398, AL1400 and AL1402) or an intact copy of fis (AL1399, AL1401 and AL1403). Brackets below the figure indicate strains derived from the same parent strain. Also shown is the amount of HA produced by the TargeTron fis mutants AL1404 and AL1405, the wild-type encapsulated parent strain VP161 and the hyaB capsule mutant AL919. Results for experimentally related strains are shaded similarly with black bars representing fis mutants, white bars representing parent and wild-type strains, hatched bars representing the fis complemented strains, gray bars representing strains transformed with vector only and the dotted bar representing the directed hyaB mutant. Each bar represents triplicate values ±1 standard error of the mean (SEM) *** p<0.001, ** p<0.01, * p<0.05.

Figure 1

doi: https://doi.org/10.1371/journal.ppat.1000750.g001