Pseudorabies Virus Infection Alters Neuronal Activity and Connectivity In Vitro
Figure 10
PRV 152 infected neurons follow a similar progression of fluorescent dye transfer and fusion of cell bodies.
(A–D) Lucifer yellow (457 MW) dye transfer between PRV 152 infected neurons was delayed until 18 hpi (9–12 hpi, 0/8, 24–26 hpi (2/4). (E–H) Large MW (≥3,000 MW) dye transfer occurred by 18–20 hpi between PRV 152 infected neurons, earlier than occurred between PRV 152 infected neurons (14–16 hpi, 0/3, 18–20 hpi, 7/9, 24–26 hpi, 9/9). (I–J) Representative fields of view of PRV 152 infected neurons and quantification. Note earlier cell body-cell body fusion between PRV 152 infected neurons compared to PRV 151 infected neurons, consistent with that observed in large MW dye experiments. (I–J) PRV 152 24–26 hpi n = 4, 40–45 hpi n = 2 and 75–78 hpi, n = 2. Data were collected displayed as described in Figures 5 and 7.