Pseudorabies Virus Infection Alters Neuronal Activity and Connectivity In Vitro
Figure 6
Large MW dye transfer between infected neurons corresponds with near complete electrical synchrony.
Dual whole cell recordings from pairs of adjacent neurons that did or did not exhibit large MW dye transfer (≥3,000 MW) at 18–20 hpi and 24–26 hpi were used to examine the electrical continuity of adjacent infected cell bodies. (A) An example pair showing neither dye transfer, nor complete electrical continuity. Time aligned traces shown are representative of those recorded. (B) An example pair showing both large MW dye transfer and complete electrical coupling. Note that in the case of large MW dye transfer, pairs of neurons showed complete electrical synchrony, including spikelet-like events and AP firing (A, grey boxes); however, when large MW dye was not transferred, spikelet-like events occurred at the same time as AP firing in the adjacent neuron (B, gray boxes). Pair described in B is example from Figure 4F.