Pseudorabies Virus Infection Alters Neuronal Activity and Connectivity In Vitro
Figure 2
Onset of PRV-induced elevated AP and spikelet-like event rates.
(A) Mean AP rates of PRV 151 infected neurons from 4 to 24 hpi. PRV 151 infected neurons fired more frequently than mock-infected neurons after 8 hpi. (B) Example whole-cell recording from a PRV 151 infected neuron at 15 hpi. Grey arrows indicate examples of spikelet-like events. These events are shown in blue, overlaid with an example of slower rising and decaying epsp in black. (C) Mean rates of spikelet-like events in PRV 151 infected neurons. PRV 151 infected neurons began to show events by 4 hpi as noted in panel A. Mock n = 51, 4–8 hpi PRV 151 n = 8, 8–10 hpi n = 8, 14–16 hpi n = 29, 18–20 hpi n = 4, and 24–26 hpi n = 14. In this and following figures asterisks (*) on bars correspond to a statistical significant difference of P<0.05 compared to the mock condition. Asterisks above brackets indicate a statistical significant difference of P<0.05 between strains. Error bars indicate standard error.