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Interactome Analyses Identify Ties of PrPC and Its Mammalian Paralogs to Oligomannosidic N-Glycans and Endoplasmic Reticulum-Derived Chaperones

Figure 1

Expression analysis of FLAG-tagged mouse prion proteins.

A, Schematic representation of murine prion proteins with FLAG tags inserted near the N-terminus. B, Expression of transiently-transfected FLAG-prion proteins in N2a cells as assessed by Western blotting with the anti-FLAG M2 antibody. The presence of a non-specific band in N2a lysates recognized by the M2 antibody is denoted by an asterisk.

Figure 1

doi: https://doi.org/10.1371/journal.ppat.1000608.g001