Intravenous Inoculation of a Bat-Associated Rabies Virus Causes Lethal Encephalopathy in Mice through Invasion of the Brain via Neurosecretory Hypothalamic Fibers
Figure 5
SB Progression within the CNS after i.m. or i.v. Inoculation.
CNS tissue from four of twelve mice infected either i.v. (panel A–D) or i.m. (panel E–H) with 107 ffu of SB was analyzed in more detail by immunohistochemical staining against RV. Animal numbers relate to Table 1. The red bar represents the section plane (stereotaxic coordinates: interaural 2.34 mm, bregma −1.46 mm) for the initial screening of all mouse brains (see text). Each vertical blue bar stands for another plane that was analyzed by several sections. The area caudal of the broken blue line represents the findings for the cervical spinal cord. The background color of each plane symbolizes its percentage of SB positive structures (nuclei, fiber tracts, cortical areas). Distinct structures were identified and counted using a stereotaxic mouse brain atlas [43]. Gray colored regions were not analyzed. The total percentage of RV immunoreactive structures for each brain is noted in the left corner of each panel.