An Unbiased Systems Genetics Approach to Mapping Genetic Loci Modulating Susceptibility to Severe Streptococcal Sepsis
Quantitative PCR results showing levels of gene expression of 14 genes with significant (P = 0.05–0.08) change post-infection in susceptible and resistant strains, expressed as Log fold differences in gene expression level post-infection. Genes are grouped to three groups, a) genes up regulated in susceptible strains post-infection and decreased in resistant strains post-infection, b) genes down regulated in both susceptible and resistant strains post-infection, and c) genes up regulated in both susceptible and resistant strains post-infection. Susceptible strains are represented as solid black bars, and resistant strains as dashed bars. The bars represent 2–4 biological replicates run in three technical replicates each and significance is based on t-test. Anapc2, anaphase promoting complex subunit 2; Entpd2, ectonucleoside triphosphate diphosphohydrolase 2; Edf1, endothelial differentiation-related factor 1; Garnl3, GTPase activating RANGAP domain-like 3; Il1a, interleukin 1 alpha; Il1rn, interleukin 1receptor antagonist; Nfatc2, nuclear factor of activated t-cells, cytoplasmic, calcineurin-dependent 2; Phpt1, phosphohistidine phosphatase 1; Ptges, prostaglandin E synthase; Ptges2, prostaglandin E synthase 2; Psmd5, proteasome (prosome, macropain) 26S subunit, non-ATPase 5; Ppp2r4, Protein phosphatase 2A regulatory subunit B; Sh2d3c, SH2 domain containing 3C; Traf1, TNF a receptor associated factor 1.