Essential Role of Domain III of Nonstructural Protein 5A for Hepatitis C Virus Infectious Particle Assembly
Figure 3
Efficiency of virus assembly and release of Jc1 carrying deletions in NS5A.
Huh7-Lunet cells were transfected with the genomes specified in the bottom and 48 h post transfection total infectious particle production was determined. Supernatants and cells were treated by freeze-thaw cycles and viral infectivity was determined by a limiting dilution assay. Mean values of four independent experiments are shown and the standard deviations of the means are presented. The grey bar represents the detection limit of the assay.