RNA Interference Screen Identifies Abl Kinase and PDGFR Signaling in Chlamydia trachomatis Entry
Figure 2
C. trachomatis infection induces phosphorylation of PDGFRβ and recruitment of phospho-PDGFRβ to the site of entry.
(A) PDGFRβ was immunoprecipitated from C. trachomatis infected HeLa cells at 1 hpi in the absence or presence of STI571 and immunoblotted with 4G10 antibody. As a positive control, cells were stimulated with 100 ng/ml PDGF-BB. (B) EGFR was immunoprecipitated from C. trachomatis infected HeLa cells at 1 hpi and immunoblotted with 4G10 antibody. As a positive control, cells were stimulated with 100 ng/ml EGF. (C) C. trachomatis infected cells incubated in the absence (panels A–C) or presence of STI571 (panels D–F) or AG1295 (panels G–I) were fixed and stained with anti-phospho-PDGFRβ antibody (green in merge). Bacteria and host DNA were detected using DAPI (blue in merge). The exposure time for each filter of all images was identical.