An RND-Type Efflux System in Borrelia burgdorferi Is Involved in Virulence and Resistance to Antimicrobial Compounds
(A) Single-channel recording of a diphytanoyl phosphatidylcholine/n-decane membrane observed in the presence of 100 ng/ml of fraction 35 of the MonoQ-FPLC of the B-fraction of B. burgdorferi B31-A p66::str. The aqueous phase contained 1 M KCl; Vm = 20 mV; T = 20°C. (B) Histogram of the probability P(G) for the occurrence of a given conductivity unit observed with membranes formed of 1% diphytanoyl phosphatidylcholine/n-decane in the presence of fraction 35 of the MonoQ-FPLC of the B-fraction of B. burgdorferi B31-A p66::str. P(G) is the probability that a given conductance increment G is observed in the single-channel experiments. It was calculated by dividing the number of fluctuations with a given conductance increment by the total number of conductance fluctuations. The average single-channel conductance for 122 single-channel events was 300 pS.