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Why is Fetal Calf Serum needed?

Posted by Neuromancer on 30 Oct 2009 at 08:57 GMT

Monocyte-derived macrophages (MDM) from healthy donors were grown in RPMI-1640 media supplemented with 10% fetal calf serum.
http://plospathogens.org/article/info:doi/10.1371/journal.ppat.1000591#article1.body1.sec2.sec4.p1

It seems that RPMI-1640 media would be sufficient. Would fetal calf serum not introduce foreign cells that would fundamentally alter the MDM's?

No competing interests declared.

RE: Why is Fetal Calf Serum needed?

sriram replied to Neuromancer on 04 Nov 2009 at 04:07 GMT

Commercially available FCS (Fetal Calf Serum) that we and other laboratories use for tissue culture does not contain blood cells. It is sterile-filtered and also heat-inactivated at 56 degrees before use. There is therefore no possibility of introducing foreign cells.

No competing interests declared.