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Question about siRNA validation in this manuscript?

Posted by Rusman on 17 Jun 2009 at 22:03 GMT

We were discussing this paper on Scientist Solutions forums recently and the following question came up.

"Also, they knocked down 727 genes that are in the kinome. They bought these siRNAs from Ambion. Check out the cost!

This is an ambitious experiment to say the least. So Im wondering how each siRNA was validated?"

Just thought Id give the authors a chance to comment.

link to discussion below (sorry if this viewed as spam)

http://www.scientistsolut...

No competing interests declared.