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Possible explanation for the lack of interaction with IN

Posted by murachel on 25 Jan 2010 at 16:52 GMT

fragment of SAP18 (aa 45–153

The NMR structure of SAP18 shows that it has a ubiquitin fold (McCallum et al., Biochemistry 2006). Based on the structure, this truncation of SAP18 lacks an entire B-strand, and might be unfolded. This would explain why the interaction with IN is not picked up in the two hybrid screen.

No competing interests declared.