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Corrigendum regarding the peptide concentration used in T cell stimulation assays

Posted by PSTEINBERG on 17 Sep 2013 at 16:15 GMT

Sentence to be corrected (in Material and Method section; page 10; Subheading: Experiments with T cells derived from HIV-1-infected Individuals):

PBMC of HIV-1-infected individuals were isolated using density gradient centrifugation and stimulated for 7 days in triplicates in flat-bottom 96-well plates with overlapping 15-mer HIV-1 Clade B Gag and Nef peptide pools (generously provided by the National Institutes of Health AIDS Research and Reference Reagent Program; final concentration 20 μg/ml).

Corrected sentence:

PBMC of HIV-1-infected individuals were isolated using density gradient centrifugation and stimulated for 7 days in triplicates in flat-bottom 96-well plates with overlapping 15-mer HIV-1 Clade B Gag and Nef peptide pools (generously provided by the National Institutes of Health AIDS Research and Reference Reagent Program; final concentration for each peptide: 1.2 μg/ml).

No competing interests declared.