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More details about experimental conditions needed

Posted by mborgnia on 15 Nov 2008 at 04:00 GMT

The pellets were resuspended in 10 µl of PBS of which 3.5 µl was placed on a 300 mesh R2/1 Quantifoil grid (Quantifoil, Jena, Germany) for 1 min. Excess virus and buffer was blotted with filter paper.
http://plospathogens.org/article/info:doi/10.1371/journal.ppat.1000203#article1.body1.sec2.sec2.p1

Temperature?
Relative Humidity?
Blotting time?
Ice thickness?