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Data inconsistency

Posted by Nina on 07 May 2008 at 09:18 GMT

In figure 5a, HIV-1 vpr-deficient vpx-supplied particles after 48 hours infect macrophages with about 2 copies per cell , whereas the same particles show only about 0.3 copies per cell in figure 6a.

What MOIs have been used in the experiments?

RE: Data inconsistency

lakecomo replied to Nina on 12 May 2008 at 21:57 GMT

Nina,
We use a standard MOI which aims to achieve one copy per cell. However, in infections of primary macrophages, one sees significant donor to donor variation in the permissivity of macrophage cultures to viral infection with the same inoculum. It is not possible to conduct multiple infections with macrophages from the same donor and, as such, variablity in the extent of infection between experiments is unavoidable.