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Publisher's Note: Statements about use of stop codons is incorrect

Posted by MWMather on 26 Sep 2008 at 18:44 GMT

primers with in frame stop codons. This fragment was cloned into pHD22Y using the Kpn I restriction enzyme site to yield pHDPKO. The stop codons in the forward and reverse primers would prevent expression of the truncated HDP in the event of site specific recombination
http://plospathogens.org/article/info:doi/10.1371/journal.ppat.1000053#article1.body1.sec4.sec6.p1

Due to incorrect interpretation of homologous recombination (see comment in discussions), authors place a stop codon preceding the truncated HDP gene in Figure 5A. There will not be a stop codon at this position following homologous recombination, hence the truncated gene will likely be expressed. The diagrams and annotations showing the anticipated outcomes of recombination in Fig 5A and B and Fig 6A and B are incorrect and need to be re-drawn.