Cryoelectron Tomography of HIV-1 Envelope Spikes: Further Evidence for Tripod-Like Legs

Open Access

Peer-reviewed

Research Article

Ping Zhu,

Affiliations Department of Biological Science and Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida, United States of America, Center for Structural and Molecular Biology and National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Chaoyang District, Beijing, China
⨯
Hanspeter Winkler,

Affiliation Department of Biological Science and Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida, United States of America
⨯
Elena Chertova,

Affiliation AIDS Vaccine Program, SAIC Frederick, National Cancer Institute at Frederick, Frederick, Maryland, United States of America
⨯
Kenneth A. Taylor,

Affiliation Department of Biological Science and Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida, United States of America
⨯
Kenneth H. Roux

* E-mail: roux@bio.fsu.edu

Affiliation Department of Biological Science and Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida, United States of America
⨯

Reader Comments

Each tilt series consisted of 70–80 images recorded over an angular range of ±60° to ±70° at increments chosen according to the cosine rule [22]. The electron dose was estimated at 1–2 e−/Å2 per image.
http://plospathogens.org/article/info:doi/10.1371/journal.ppat.1000203#article1.body1.sec2.sec3.p1

Underfocus?

SIV
Is the Subject Area "SIV" applicable to this article?

Thanks for your feedback.
HIV-1
Is the Subject Area "HIV-1" applicable to this article?

Thanks for your feedback.
Viral structure
Is the Subject Area "Viral structure" applicable to this article?

Thanks for your feedback.
Virions
Is the Subject Area "Virions" applicable to this article?

Thanks for your feedback.
Sequence alignment
Is the Subject Area "Sequence alignment" applicable to this article?

Thanks for your feedback.
Atomic structure
Is the Subject Area "Atomic structure" applicable to this article?

Thanks for your feedback.
HIV vaccines
Is the Subject Area "HIV vaccines" applicable to this article?

Thanks for your feedback.
Tomography
Is the Subject Area "Tomography" applicable to this article?

Thanks for your feedback.