TY - JOUR T1 - Coronavirus Gene 7 Counteracts Host Defenses and Modulates Virus Virulence A1 - Cruz, Jazmina L. G. A1 - Sola, Isabel A1 - Becares, Martina A1 - Alberca, Berta A1 - Plana, Joan A1 - Enjuanes, Luis A1 - Zuñiga, Sonia Y1 - 2011/06/09 N2 -
Innate immune response is the first line of antiviral defense. Viruses have developed diverse strategies to evade this deleterious response, ensuring their survival. Several CoV accessory genes play a central role in these pathways. Nevertheless, the molecular mechanisms by which they exert their function are still unknown. The generation of a rTGEV without gene 7 expression allowed us to study the role of protein 7 in the modulation of the antiviral response. The absence of protein 7 during TGEV infection caused an enhanced apoptosis and a translational shutoff, due to an increased cellular RNA degradation and eIF2α phosphorylation. We identified a protein phosphatase 1 (PP1) binding motif in protein 7, and a TGEV protein 7-PP1 interaction was demonstrated. We propose a novel mechanism to counteract dsRNA-induced antiviral response by RNA viruses.