TY - JOUR T1 - Ago HITS-CLIP Expands Understanding of Kaposi's Sarcoma-associated Herpesvirus miRNA Function in Primary Effusion Lymphomas A1 - Haecker, Irina A1 - Gay, Lauren A. A1 - Yang, Yajie A1 - Hu, Jianhong A1 - Morse, Alison M. A1 - McIntyre, Lauren M. A1 - Renne, Rolf Y1 - 2012/08/23 N2 - Author Summary Kaposi's sarcoma-associated herpesvirus is the etiological agent of KS and two lymphoproliferative diseases: multicentricCastleman's disease and primary effusion lymphomas (PEL). KSHV tumors are the most prevalent AIDS malignancies and within Sub-Saharan Africa KS is the most common cancer in males, both in the presence and absence of HIV infection. KSHV encodes 12 miRNA genes whose function is largely unknown. Viral miRNAs are incorporated into RISCs, which regulate gene expression mostly by binding to 3′UTRs of mRNAs to inhibit their translation and/or induce degradation. The small subset of viral miRNA targets identified to date suggests that these small posttranscriptional regulators target important cellular pathways involved in pathogenesis and tumorgenesis. Using Ago HITS-CLIP, a technique which combines UV cross-linking, immunoprecipitation of Ago-miRNA-mRNA complexes, and high throughput sequencing, we performed a detailed analysis of the KSHV miRNA targetome in two commonly studied PEL cell lines, BCBL-1 and BC-3 and identified 1170 and 950 putative miRNA targets, respectively. This data set provides a valuable resource to decipher how KSHV miRNAs contribute to viral biology and pathogenesis. JF - PLOS Pathogens JA - PLOS Pathogens VL - 8 IS - 8 UR - https://doi.org/10.1371/journal.ppat.1002884 SP - e1002884 EP - PB - Public Library of Science M3 - doi:10.1371/journal.ppat.1002884 ER -