Skip to main content
Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

  • Loading metrics

Not stealing from the treasure chest (or just a bit): Analyses on plant derived writing supports and non-invasive DNA sampling

  • Anna Schulz,

    Roles Conceptualization, Investigation, Methodology, Validation, Writing – original draft

    Affiliation Hamburg School of Food Science, University of Hamburg, Hamburg, Germany

  • Silke Lautner,

    Roles Investigation, Methodology, Visualization

    Affiliation Centre for Wood Science, University of Hamburg, Hamburg, Germany

  • Jörg Fromm,

    Roles Funding acquisition, Methodology, Visualization, Writing – original draft

    Affiliation Centre for Wood Science, University of Hamburg, Hamburg, Germany

  • Markus Fischer

    Roles Conceptualization, Funding acquisition, Project administration, Supervision

    markus.fischer@uni-hamburg.de

    Affiliation Hamburg School of Food Science, University of Hamburg, Hamburg, Germany

Abstract

Written communication plays a crucial role in the history of modern civilizations as manuscripts do not only exist contemporarily, but are passed on to subsequent generations. Besides a document’s content, information is stored in the materials used for its production. Analyses of the composition allow, for example, identifying the biological origins of materials, dating, and help to understand degradation patterns. A combination of microscopic and DNA approaches was applied in order to analyze various plant derived writing sheets. Given their diversity and abundance in museum collections, plant based writing supports are yet an underexplored target for DNA studies. DNA retrieval of paper is low compared to raw paper plant material, which is likely due to the loss of organic components during paper production. Optimizing DNA extraction for each respective material drastically increased DNA recovery. Finally, we present a non-invasive DNA sampling method that utilizes nylon membranes, commonly used for bacterial DNA sampling and that is applicable to delicate material. Although bacterial infestation was visible on one sample, as indicated by scanning electron microscopy, endogenous DNA was retrieved. The results presented here are promising as they extend the scope of sources for DNA analyses by demonstrating that DNA molecules can be retrieved from a variety of plant derived writing supports. In future, such analyses can help to explore the biological diversity not only of plants and of additives utilized for producing writing supports, but also of the plenty products made from paper.

Introduction

Throughout history, versatile materials were utilized as writing support, starting with Paleolithic cave rocks to Bronze Age clay tablets to Iron Age parchment, modern paper, and novel stone paper. Besides a document’s content, information is stored in the materials used for its production. Analyses of the composition allow, for example, identification of materials [17], dating [8, 9], and help to understand degradation patterns [6, 10, 11]. Given their abundance in museum collections and the diversity of writing supports made of plant material, such as papyrus scrolls, wood tablets, and paper, with one exception [12], plant derived manuscripts are an underexplored target for DNA analyses. In theory, DNA molecules can be obtained from any organic material such as bone [13, 14], eggshell [15], wood [16, 17], as well as from processed material, for example food [18, 19], clothing [2022], and parchment [14, 23]. The survival and preservation of DNA molecules is not only influenced by the age of a sample, but to a higher degree by the organic source [2426] and the environment [27, 28], with arid conditions and low temperature fluctuations favoring long time DNA survival. For processed material, the manufacturing process will additionally influence molecular preservation [7, 21, 23]. Preparing manuscript sheets from a variety of organic materials involves physical force (cutting and pressing) as well as thermal and chemical treatment (soaking or boiling in alkaline solutions). Sheets were often bleached, tanned or coated with animal or plant derived sizes for improved ink absorption [29, 30]. Because the production of paper requires mainly cellulosic material and water, paper can be and has been manufactured not only from fresh bark and wood, but also from textile waste, such as linen, hemp, and cotton [30].

Another limitation for DNA analyses is the sensitivity of the applied method, especially during the isolation of DNA molecules. Protocols do exist for the isolation of DNA molecules from fresh and herbarium leaf samples [24, 31, 32], but their efficiency to retrieve DNA from the diverse plant derived writing supports has not been tested. Palm leaves, bark, and pith- the raw material for papyrus- are lignified and are probably more difficult to dissolve in order to release DNA molecules. Concerning cultural heritage, research is restricted by the need of material integrity. DNA analyses are usually invasive, as they require removal of material. In order to study precious samples, such as museum collections, rare or small specimens, it is necessary to develop non-destructive sampling techniques. Non-destructive sampling can be thought of removing sampling material while keeping the material intact. For example, incubating material in lysis buffer was successfully performed for sampling DNA from bones [33] and insects [34, 35], while scraping was applied for sampling residues in ceramics found in a shipwreck [36]. Non-invasive or minimally invasive sampling will remove sampling material without penetrating the surface of a material, for example by using swabs. Recent studies have demonstrated the ability of analyzing parchment [37] and herbarium specimens [31] by applying eraser sampling, although it has been noted, that this method is not applicable to delicate samples, as they become easily damaged [31]. The usage of binding membranes offers another non-invasive sampling method. Nitrocellulose and nylon membranes have been applied for bacterial and fungal sampling from the surface of paintings [38], manuscripts [39], and photographs [40] so far, but have not been tested in their efficiency to bind endogenous DNA.

Here, we have analyzed samples of Asian paper, papyrus, and a historic palm leaf manuscript by using different extraction protocols. In order to monitor the effect of paper production on DNA survival, material of paper mulberry (Broussonetia papyrifera) was analyzed at different manufacturing steps (raw bark, cooked bark, modern unbleached and bleached paper sheets, and an early 20th century unbleached paper sheet). We successfully tested a non-invasive DNA sampling technique that is applicable to delicate material, such as Asian paper. We identified the biological origin of the 18th century palm leaf manuscript by independent DNA and microscopy approaches. In particular, light microscopy and scanning electron microscopy (SEM) were applied, two independent and widespread tools to analyze plant structure. Light microscopy provides a rapid method and is used to study cells of the order of magnitude 1 μm to several mm. For the analyses of leaf samples it provides information on tissue patterns, cell types, and for species identification [41]. In contrast to optical microscopy, SEM reaches much higher magnification and has been used over the years for looking at numerous aspects of plant structure, for example pollen grains [42], plant cell walls [43], and leaf surfaces [44].

Results and discussion

Effect of paper production

In brief, the manufacture of Asian bark paper involves watering or cooking of raw bark in the presence of alkaline additives. The fibers then are washed, separated manually or mechanically by beating, and mixed with water. The resulting pulp is poured on a casting mold and left to dry [45]. Based on a total of 11 different extraction protocols the average DNA content (given as ng DNA per mg of input tissue) was calculated for bark paper at different production steps (raw bark, cooked, bark, unbleached paper, bleached paper). Major differences in DNA content were observed between raw bark material and processed paper. Cooked bark yielded more DNA than raw bark tissue (on average 26.3 and 10.6 ng/mg, respectively, t test p-value = 0.001, see Tables 1 and 2), which can be most likely attributed to incomplete tissue disintegration during lysis. Dry bark is extremely hard and dense, and dissolved hardly after 24 h incubation in lysis buffer. Because of cooking, the release of DNA molecules might be facilitated, while raw material needs prolonged incubation in order to disrupt the tissue matrix. Homogenizing raw bark material in lysis buffer for 72 h led to a significant increase in the DNA output (69.5 ng/mg, t test p-value = 0.05). Significant differences in PCR success were observed between raw and cooked material with respect to amplicon length. Cooked bark showed lower amplification success for longer fragments (i.e. 791 base pairs (bp) and 500 bp, t test p-values = 0.006). Depending on the plant source, cooking of raw bark material can take up to several hours [45] and heat induced fragmentation of DNA molecules [46] during this step of paper preparation maybe a reason.

thumbnail
Table 1. Average DNA yield and amplification success of Broussonetia papyrifera at different paper production steps.

PCR success is given as the number of PCR products (visualized by gel electrophoresis) for different amplicon lengths (791, 500, and 120bp). Standard deviation for DNA yields is given in parentheses.

https://doi.org/10.1371/journal.pone.0198513.t001

thumbnail
Table 2. Results of t tests for paper manufacturing experiments.

p-values< 0.05 are highlighted. n = sample number.

https://doi.org/10.1371/journal.pone.0198513.t002

To test whether DNA degradation had an effect on the differences in amplification success, DNA sequence lengths were measured on a Bioanalyzer. No shifts in fragment lengths were observed between raw and cooked bark as well as modern, unbleached paper, while measurements of bleached paper failed to produce a measurement. Rather than fragmentation, it appears that alkaline chemicals added during cooking inhibit PCR amplification.

DNA yield was significantly lower in paper compared to cooked bark (t test p-value = 0.001), which is most likely attributed to a loss of organic compounds during pulping. Fibers consist of cellulose, lignin and hemicelluloses. A high content of cellulose is the key determinant of paper strength. In order to obtain cellulose, chemical (using alkaline solutions) and/or mechanical (by beating or grinding) pulping is performed, which results in the dissolution of nearly all lignin and more than half of the hemicellulose content from the fibers, whereas cellulose is partly degraded. During the removal of lignin and hemicellulose, the pore volume increases and the fiber surface becomes more open (Fig 1) [47]. Lignin is hydrophobic and is associated with material durability [48], protection of cellulose and hemicellulose from degradation, protection of cell walls [49], and pathogen resistance [50], and may be linked also to the good molecular preservation of the historic palm leaf (see below) and other lignified, ancient tissues [16, 5153].

thumbnail
Fig 1. SEM images from Broussonetia papyrifera.

(A) Raw bark material. Overview of the transverse section. Within the various cell types (parenchyma cells, fibers, sieve elements, companion cells) fibers had the highest content (> 50%). (B) Cooked bark material. Numerous bends are evident and the fibers appear more or less wrinkled. (C). Unbleached paper showing several mm long fibers. (D) Detailed view on the fiber surface showing several bends and variations in fiber thickness from 3 to 15 μm.

https://doi.org/10.1371/journal.pone.0198513.g001

Within paper, DNA content was highest in unbleached samples (3.9 ng/mg for modern paper and 5.7 ng/mg for the historic specimen). Due to limited sample amount, DNA extraction was performed only twice for the historic paper specimen including the protocol that obtained the highest DNA yield for paper (EDTA+ NaCl+ SDS lysis). The DNA yield of the historic paper specimen did not differ significantly to modern, unbleached paper (5.6 ng/mg) when comparing the same extraction methods only (t test p-value = 0.930). Although a bias due to limited analyses cannot be rejected for the historic paper sample, one should keep in mind that historic and modern paper production differ in their level of chemical input. Traditionally, water filtered through ashes is used for cooking bark material and is nowadays replaced by adding alkaline chemicals, such as potassium carbonate.

Bleaching had a negative effect on both DNA recovery (2.2 ng/mg compared to 3.9 ng/mg, t test p-value = 0.006) and overall PCR amplification success (6–27% compared to 30–45% for unbleached modern paper, t test p-values< 0.05, Tables 1 and 2). The paper sheet analyzed here was treated with chemical bleach, which is likely to be more detrimental to DNA survival than traditional bleaching by sun or snow.

DNA fragments of the historic paper sample ranged around 80 base pairs (bp). Given the moderate age of this sample (early 20th century), this finding suggests that DNA fragmentation occurs relatively fast. This was also observed in the palm leaf manuscript analyzed here (c. 140 bp), and in accordance to previous studies on historic specimens of younger date [24, 54, 55].

DNA recovery of plant derived manuscripts using different lysis buffers

A total of eleven lysis buffers and varying incubation times were tested in order to identify the best method for each respective plant derived writing support (modern papyrus, modern unbleached paper, historic palm leaf manuscript) and other plant material (fresh soft leaves and raw bark). Within samples of the same material (i.e. raw bark, leaves, modern, unbleached paper), significant differences in the DNA yield were only observed between fresh, alcohol preserved leaves (t test p-value = 0.002, see Table 3). The leaves were sampled and analyzed at the same time, and were preserved and stored under the same conditions. It therefore seems unlikely that these factors had an effect on the results.

thumbnail
Table 3. T test results of extraction comparisons.

p-values< 0.05 are highlighted. n = sample size.

https://doi.org/10.1371/journal.pone.0198513.t003

CTAB-based lysis buffers, commonly used for materials rich in polyphenols and polysaccharides [56], yielded the highest DNA amounts for fresh leaves and bark material (199.9 ng/mg and 24.4 ng/mg, respectively, Table 4). Buffers containing mainly EDTA, a chelating agent that prevents DNA degradation by nucleases and is used for decalcification during DNA extraction from bones, yielded highest DNA amounts for paper and the palm leaf. For papyrus no significant difference was observed between CTAB or EDTA based lysis buffers (6.01 ng/mg and 7.5 ng/mg, respectively, t test p-value = 0.223), but a significant increase in DNA yield was observed in comparison to the PeqGOLD Plant DNA kit (t test p-value = 0.003, Table 3).

thumbnail
Table 4. DNA yield using different extraction lysis buffers and amplification success.

n = sample size. Each extraction method was tested six times on each material. PCR and qPCR were conducted in triplicate for each sample. Standard deviation is given in parentheses. PCR success is given as the number of PCR products (visualized by gel electrophoresis) for different amplicon lengths (791 bp, 500 bp, and 114/ 120 bp). n.a. = not analyzed.

https://doi.org/10.1371/journal.pone.0198513.t004

Within writing supports, palm leaf showed the best results in terms of DNA retrieval and PCR amplification success (up to 138.2 ng/mg and 100% amplification success irrespective of extraction method, see Table 4), followed by papyrus (16.4 ng/mg) and paper (8.7 ng/mg). Although assessment of DNA quantity using fluorescent dyes is a widely used approach, there are some drawbacks in accuracy. Unlike quantification via UV spectrophotometry (such as Nanodrop), fluorometric measurements seem not to be influenced by the presence of protein or RNA contaminants [57], but will underestimate DNA quantity in case of DNA fragmentation [58]. An underestimation of DNA yield in case of fragmented historic samples therefore cannot be excluded.

A negative correlation was observed between DNA yield and PCR amplification success for paper. Using EDTA as main component of the lysis buffer, DNA yield was significantly higher (t test p-value = 0.003, Table 3), but overall PCR performance dropped (t test p-values = 0). While no difference in PCR performance was detected for the shorter fragment (i.e. 120 bp), longer fragments were more often successfully amplified using CTAB as main lysis buffer component for paper (44% compared to 27% for the 500 bp fragment and 40% compared to 22% for the 791 bp fragment, see Table 4). With the exception of papyrus, amplification performance in general was better when CTAB was used as main component of the lysis buffer (t test p-values< 0.045), maybe because CTAB is more efficient in removing polyphenols and polysaccharides which are known to inhibit PCR amplification [59]. Spiking PCR of a subset of EDTA lysed samples did not indicate inhibition. Also diluting DNA template did not enhance PCR success. EDTA has an inhibitory effect on PCR performance, as it depletes divalent cations, such as Mg2+, necessary for PCR performance. Depending on the concentration of EDTA, the inhibitory effect will occur randomly, though it has also been noted that some amplicons may be more susceptible to inhibition [60].

Sufficient amounts of amplifiable plant DNA were measured irrespective of the applied extraction method (indicated by Cq values below 29, Fig 2). Cq values were lower for unprocessed material than for writing supports.

thumbnail
Fig 2. Cq values obtained for the rbcl region from different extraction methods.

Each extract was tested in triplicate for the plant specific rbcl region.

https://doi.org/10.1371/journal.pone.0198513.g002

DNA purity was assessed using the absorbance ratio A260/A280. While a ratio of ~1.8 indicates pure DNA and higher ratios are indicative of the presence of RNA, lower ratios point to the presence of impurities by proteins or phenol. Sample purity was comparable between the Plant DNA kit and CTAB based lysis buffer (1.5 to 1.9 and 1.4 to 2.1, respectively), while EDTA based lysis buffers showed the lowest sample purity (as low as 0.55, Table 4). A comparison of amplification success of extracts with low DNA purity (≤1.65) to amplification success of DNA extracts with high purity (= 1.8 ±0.02) did not indicate a correlation between purity and PCR success, similar to findings in other studies [61].

Overestimation of DNA retrieval due to the presence DNA contaminations was tested by targeting 123 bp of bacterial 16S rRNA. Real-time qPCR did not indicate a bias in the fluorometric measurements of DNA yields. Rather, bacterial DNA was co-extracted in equally high amounts independent of the extraction method (Fig 3).

thumbnail
Fig 3. Cq values obtained for bacterial 16S rRNA from writing supports.

Each extract was tested in triplicate.

https://doi.org/10.1371/journal.pone.0198513.g003

Using the best protocol in terms of DNA retrieval for each respective plant based writing support and changing lysis time (6 h at 37 °C, and 48 h and 72 h, each at 25 °C) had a negative effect on DNA yield (t test p-values< 0.05) as well as PCR amplification success after 72 h incubation (t test p-value = 0.010). One possible explanation is DNA degradation due to components of the lysis buffer.

Minimally destructive and non-invasive sampling of endogenous DNA

The amount of sample material used for DNA extraction was reduced in the course of the experimental set up to 1 mg. In case of papyrus and the historic palm leaf, lower material input correlated with higher DNA yields (t test p-values< 0.05, Table 5). The volume of lysis buffer was probably too low for the lysis of higher material amounts (i.e. 10 mg) and might need to be adjusted in case more material is used for extraction. For the palm leaf manuscript, this corresponded to approximately 2 x 2 mm and produced up to 158 (±38.2) ng DNA. For papyrus, 1 mg corresponded to c. 2 x 3 mm and produced up to 21.75 (±2.5) ng DNA. For paper, 1 mg of material corresponded to approximately to 4 x 5 mm and yielded up to 11.1 (±2.4) ng.

thumbnail
Table 5. T test results on the influence of material input on DNA output.

p-values< 0.05 are highlighted. n = sample size.

https://doi.org/10.1371/journal.pone.0198513.t005

Although non-destructive sampling is the method of choice for studying cultural heritage and small specimens, invasive sampling is preferable due to higher DNA recovery and a lower risk of extracting contaminant DNA. Especially writing supports will be exposed to high levels of human contamination introduced during manufacturing and usage, and may additionally contain other organic sources involved in the manufacturing of sheets (e.g. glues, sizes).

DNA sampling using eraser proved to be a valuable method for the analysis of parchment [37] and herbaria [31]. Although no DNA was measured after DNA extraction, eraser sampling produced PCR amplification products and authentic sequences for the palm leaf manuscript, while amplification failed for papyrus. In case of paper, eraser sampling proved not to be non-invasive. Asian paper is of filigree texture and rubbing resulted in the damage of the surface.

Using positively charged nylon membranes, low amounts of DNA were recovered from all plant derived writing supports as long as membranes were moistened prior to sampling (up to 0.18 ng/μl for paper, 0.06 ng/μl for papyrus, and 0.4 ng/μl for the historic palm leaf manuscript, Table 6).

thumbnail
Table 6. Results of non-invasive DNA sampling of writing supports.

n = sample size. Each sampling method was tested twice on each material. PCR was conducted in triplicate for each sample. PCR success is given as the number of PCR products (visualized by gel electrophoresis) for different amplicon lengths (791 bp, 500 bp, and 114/ 120bp). n.a. = not analyzed.

https://doi.org/10.1371/journal.pone.0198513.t006

Blank controls introduced during sampling (i.e. nylon membranes), DNA extraction and PCR amplification did not indicate contamination. Binding membranes have been so far applied for bacterial and fungal sampling from various surfaces [3840]. The risk of sampling microbial DNA from the palm leaf manuscript was valid as SEM analyses showed fungal infestation (Fig 4D). Anyway, all sequences retrieved via non-destructive sampling from this sample matched the identified species (see below). For the papyrus sheet, four endogenous sequences out of five were retrieved using nylon sampling, while one contaminant sequence belonged to muskmelon (Cucumis melo). The origin of this contamination is unknown. Muskmelon has not been analyzed in the laboratory facilities, therefore contamination during DNA sampling and analysis seems unlikely. For the paper sample made of Broussonetia papyrifera, sequences of Aloe vera were also retrieved. Aloe was used as sizing agent during paper production and sequences of aloe were also recovered using destructive DNA sampling.

thumbnail
Fig 4. SEM images from the lamina of a palm leaf.

(A) Overview of a small leaf sample. (B) The transverse cut direction (above) and the lower leaf surface show the typical structure of a monocotyledonous leaf with parallel lines of stomata that are responsible for gas exchange. (C) A veinlet in the middle of a transverse section shows the xylem and the phloem surrounded by the sclerenchyma sheath and ground tissue. (D) Fungal hyphae occur within the various cell types of the leaf.

https://doi.org/10.1371/journal.pone.0198513.g004

Biological origin of the historic palm leaf manuscript.

In case of the palm leaf manuscript, species was identified also using light microscopy and SEM. Light microscopy yielded a complete overview of the leaf from the historic manuscript (Fig 5), in agreement with the structure of a palm leaf taken from a herbarium. Species-specific features within the leaf tissues and on the leaf surface were used to determine the affiliation to palmyra palm (Borassus flabellifer). Results were confirmed using SEM (Fig 5). Palmyra palm is one of two major plant species (the other being the talipot palm, Corypha umbraculifera) used for the production of palm leaf manuscripts in South Asia.

thumbnail
Fig 5. Light microscopic image of a transverse section of a historic manuscript sheet of palm leaf (Borassus flabellifer).

The dermal system comprises the epidermis (E). The chlorenchymatous ground tissue consists of mesophyll (M) while the vascular system is represented by xylem (X), which is responsible for water flow, and phloem (P), required for assimilate transport. These vascular tissues are stabilized by a sclerenchyma sheath (S).

https://doi.org/10.1371/journal.pone.0198513.g005

DNA analysis for the identification of paper sources.

While microscopic analyses will be sufficient for the identification of plants used for producing writing supports in many cases, some plant fibers cannot be distinguished easily. For example the fibers of Daphne and Edgeworthia, two plants used for paper production in the Himalayan region [45]. Comparing 748 bp of the chloroplast rbcl (RuBisCo, ribulose-1,5-bisphoshate carboxylase/oxygenase) region of D. bholua and E. gardneri with D. kiusiana [62] and E. chrysantha [63], two other species of Daphne and Edgeworthia, 12–13 variable positions were found and only one variable position within each genus. We conclude that DNA analyses can be applied to distinguish between paper sheets made of the respective plants.

Conclusions

Our results indicate, that sufficient DNA can be retrieved from a variety of plant derived manuscripts. Especially the palm leaf manuscript analyzed here showed a good molecular preservation. For paper, it appears that both loss of DNA material as well as some degree of heat induced fragmentation occur during the production of paper. Given that modern paper manufacturing is more detrimental due to the usage of industrial chemicals (i.e. usage of chemicals such as potassium carbonate instead of plant ash as alkaline additive and usage of chemical bleach instead of sun or snow bleaching) our results are promising for future studies on historic paper specimen. Paper products exist in abundance and have been used for purposes other than writing, such as armor, wrapping, and money. Lastly, we show that alternative sampling methods can be applied for analyzing writing supports. Positively charged nylon membranes were successfully applied for nondestructively sample endogenous DNA from delicate material.

Materials and methods

Material

Sample material for this study was collected from several plant species used for the production of Asian paper and is listed in Table 7. Modern paper sheets were produced at local workshops. A papyrus sheet was freshly prepared for analysis. Palm leaf of unknown biological origin was directly removed from an 18th century manuscript from a private collection.

thumbnail
Table 7. Overview of the samples and DNA methods applied.

https://doi.org/10.1371/journal.pone.0198513.t007

All samples were stored at room temperature until further processing.

Methods

Invasive sampling and DNA extraction.

Sample preparation and DNA analyses took place at the laboratories of the Hamburg School of Food Science, University of Hamburg.

Prior to DNA extraction, 1 to 30 mg of material was removed using sterile scissors and forceps and cut into small pieces if necessary. In order to test the best extraction method for each respective plant material eleven lysis buffers were compared. Of these, five lysis buffers included cetyl trimethylammonium bromide (CTAB) as main agent, which is commonly used for plant DNA extraction; five buffers included ethylendiaminetetraacetic acid (EDTA) as main component. The method that produced the best result in terms of DNA retrieval for plant derived writing supports was repeated with varying incubation times (48 h and 72 h, each at 25 °C, and 6 h at 37 °C). Lysis buffers used for comparison are given below.

Examination of the influence of paper production on DNA preservation was performed on all sources of Broussonetia papyrifera (raw and cooked bark, modern bleached an unbleached paper, historic unbleached paper) with the extraction methods given below. The only exception was the historic paper manuscript. Due to limited material only two lysis buffers were tested on this sample (PeqGOLD Plant DNA Mini kit and EDTA + NaCl + SDS).

PeqGOLD Plant DNA Mini kit modified: 10 to 30 mg of sample material was extracted using the PeqGOLD Plant DNA Mini kit (Peqlab/VWR, Darmstadt Germany) following the manufacturer’s instruction except for an extended incubation time in 500 μl of Lysis Buffer PL1 and 15 μl RNase A for 24 h at 55 °C. DNA material was eluted in 80 μl in two steps of centrifugation after 5 minutes of incubation at 37 °C.

CTAB (modified after [64]): 1 to 10 mg of sample material was incubated in 500 μl of lysis buffer (2% (w/v) CTAB, 100 mM Tris-HCL pH 8.0, 20 mM EDTA pH 8.0, 1.4 M NaCl, 5 μl/ml β-mercaptoethanol, 40 mg/ml PVP) followed by chloroform extraction and isopropanol precipitation. Differences to [64] were an extended incubation time for 24 h at 55 °C in constant agitation and a second extraction with one volume of chloroform. Samples were resuspended in 50 μl of ultrapure water.

CTAB + SDS (modified after [65]): 1 to 10 mg of material was soaked in 600 μl lysis buffer (2% (w/v) CTAB, 100 mM Tris-HCL pH 8.0, 20 mM EDTA pH 8.0, 1.4 M NaCl, 20 mg/ml PVP) and 5 μl of β-mercaptoethanol. After 1 h incubation at 55 °C, SDS was added to each sample to a final concentration of 0.3%. Differences to [65] were less amount of plant material, an extended incubation time in lysis buffer for 24 h at 55 °C in a thermomixer, and two rounds of chloroform extraction. DNA was precipitated in 0.02 volumes of 5 M NaCl and 0.54 volumes of isopropanol followed by two rounds of washing in 70% ethanol. Samples were resuspended in 50 μl of ultrapure water.

CTAB + DTT + SDS: 660 μl of lysis buffer (2% (w/v) CTAB, 100 mM Tris-HCL pH 8.0, 20 mM EDTA pH 8.0, 1.4 M NaCl, 50 mM DTT, 40 mg/ml PVP, 1% SDS) was added to 1 to 10 mg of sample material and incubated for 24 h at 55 °C. Samples were purified twice with one volume of chloroform followed by precipitation as outlined above.

CTAB + silica column: 1 to 10 mg of material was dissolved as described in the CTAB+ DTT+ SDS protocol. After 24 h incubation at 55 °C, samples were centrifuged for 10 minutes at 15.000 x g. The supernatant was transferred to microfilters provided in the PeqGOLD Plant DNA Mini Kit and samples were processed according to manufacturer’s instructions. Samples were eluted in two steps of centrifugation after 5 minutes of incubation at 37 °C in a total of 60 μl of elution buffer.

CTAB + BME + DTT + SDS: 660 μl of lysis buffer (2% (w/v) CTAB, 100 mM Tris-HCL pH 8.0, 20 mM EDTA pH 8.0, 1.4 M NaCl, 40 mM DTT, 5 μl/ml β-mercaptoethanol, 40 mg/ml PVP, 1% SDS) was added to 1 to 10 mg of sample material. After 24 h at 55 °C in a heating block, chloroform extraction and precipitation with isopropanol and NaCl were performed as described above.

EDTA + DTT + Proteinase K + SDS: 1 to 10 mg sample material was digested in 650 μl lysis buffer (0.45 M EDTA pH 8.0, 50 mM DTT, 0.25 mg/ml Proteinase K, 1% SDS) for 24 h at 37°C in constant agitation followed by chloroform extraction and precipitation as detailed above.

EDTA + NaCl + DTT+ SDS: 1 to 10 mg sample material was digested in 650 μl lysis buffer (0.43 M EDTA pH 8.0, 0.25 M NaCl, 50 mM DTT, 1% SDS) for 24 h at 37 °C in constant agitation followed by chloroform extraction and precipitation as described above.

EDTA + DTT + SDS: 1 to 10 mg sample material was digested in 650 μl lysis buffer (0.44 M EDTA pH 8.0, 50 mM DTT, 2% SDS). After 24 h at 37 °C in constant agitation, a chloroform extraction was performed and samples were precipitation with isopropanol as described above.

EDTA + NaCl + SDS: 1 to 10 mg sample material was digested in 650 μl lysis buffer (0.43 M EDTA pH 8.0, 0.25 M NaCl, 2% SDS) for 24 h at 37 °C in constant agitation followed by chloroform extraction and precipitation as outlined above.

EDTA + SDS: 1 to 10 mg sample material was digested in 650 μl lysis buffer (0.45 M EDTA pH 8.0, 2% SDS) for 24 h at 37 °C in constant agitation followed by chloroform extraction and precipitation with isopropanol as described above.

Non-destructive sampling and DNA extraction.

Collection of eraser crumbs (c. 15 mg; Mars®plastic, Staedtler) was performed as described in [2] with the exception, that eraser crumbs were not collected on paper sheets but were transferred directly from the sampling material into 1.5 ml tubes.

DNA sampling via positively charged nylon membranes (Nytran®SuPerCharge, Whatman, GE Healthcare) was performed once with dry membranes and once with membranes moistened with 0.5 M EDTA (pH 8.0). Membranes were cut into pieces (c. 1 x 2 cm), pressed for 30 s on the material and transferred to 2 ml tubes containing lysis buffer.

Eraser crumbs and nylon membranes were incubated for 3h at 55 °C in a lysis buffer containing 0.67 M EDTA (pH 8.0) and 1% SDS and were extracted using one volume of chloroform and isopropanol precipitation as outlined above.

DNA quantification and fragment size determination.

DNA amount was measured using a Quantus Fluorometer and QuantiFluor ds DNA kit (Promega, Mannheim, Germany). Real-time PCR was used to assess the amount of DNA in each of the eleven extraction methods and was performed in triplicate for each writing support, as well as bark and leaf samples from D. bholua. Presence of microbial DNA was tested for each of the eleven extraction methods in triplicate for each writing support (historic palm leaf manuscript, modern papyrus sheet and unbleached paper made of D. bholua). Purity of DNA extracts (OD. 260/280) was measured using a Nanodrop ND-1000 spectral photometer (Thermo Fisher Scientific). PCR amplification success was determined by gel electrophoresis. Fragment sizes were measured on the Bioanalyzer 2100 (Agilent) using the high sensitivity kit.

PCR amplification and spiking PCR.

Primers were designed using Primer-BLAST [66] and reference sequences for rbcl (RuBisCo, ribulose-1,5-bisphoshate carboxylase/oxygenase) of various plants deposited in GenBank (Broussonetia papyrifera [access. no. AF500347], Daphne mezerum [access. nos. AF022132, AJ297233], Daphne laureola [access. no. HM849946], Edgeworthia chrysantha [access. nos. AJ297920, KP088576][63, 6770], Borassus flabellifer [access. nos. KP901247, AY012469], Corypha umbraculifera [access. no. AJ404761], Corypha taliera [access. no. AJ404762], Corypha utan [access. no. AY012466], Cyperus papyrus [access. no. Y12966], Cyperus involucratus [access. no. Y12967], Cyperus alternifolius [access. no. HQ182424]) [63, 6775]. For the palm leaf manuscript, whose biological origin was unknown prior to analysis, sequences of two palm species used for manuscript production (Borassus and Corypha) was compiled. For Daphne bholua and Edgeworthia gardneri no reference sequences were available. In order to generate primers, sequences of related species (D. laureola, D. mezerum, and E. chrysantha) were taken. To account for polymorphic positions at binding sites, primer sequences included ambiguous bases [76]. A scheme of the primer design is shown in Fig 6.

Reference sequences of D. bholua and E. gardneri spanning 748 bp of the chloroplast rbcl region were compiled using either two overlapping primer sets (rbcl_1F/rbcl_1R and rbcl_PAPF1/rbcl_PAPR2), or one primer pair (rbcl_1F/rbcl_PAPR2).

PCR performance of the different extraction methods was tested for modern paper plant samples (all material belonging to either Daphne, Broussonetia, or Edgeworthia) using three different primer sets (rbcl_PAPF1/ rbcl_PAPR1, rbcl_1F/ rbcl_1R, and rbcl_1F/ rbcl_PAPR2), that amplify 120bp, 500bp, and 791bp of the rbcl region. For papyrus, rbcl_PAPF1/rbcl_PAPR1 was substituted by rbcl_PMF1/ rbcl_PMR1. Due to fragmentation, only short amplicons were tested for historic samples. Primers are listed in Table 8.

thumbnail
Table 8. Overview of the primer sequences and amplicon lengths.

https://doi.org/10.1371/journal.pone.0198513.t008

For modern material PCR was set up with 1.2X DreamTaq Buffer (Thermo Fisher Scientific, containing 2.4 mM MgCl2), 1 U DreamTaq Polymerase (Thermo Fisher Scientific), 0.4 μg/μl BSA, 0.2 mM dNTP mix (each), 0.1–0.4 μM each primer, and 0.5 to 3 μl of DNA template in a final volume of 20 μl. Initial denaturation for 2 min at 94 °C was followed by 33 cycles of 30 s at each 94 °C, 56 °C and 72 °C, and a final elongation at 72 °C for 10 min.

For historic material PCR reaction was carried out in a final volume of 20 μl containing 1.2x PCR Gold Buffer (Applied Biosystems, Thermo Fisher Scientific), 3 mM MgCl2 (Applied Biosystems, Thermo Fisher Scientific), 2.5 U AmpliTaq Gold (Applied Biosystems, Thermo Fisher Scientific), 0.4 μg/μl BSA, 0.2 mM dNTP mix (each), 0.2 μM of each primer, and 1 to 3 μl template DNA. Cycling conditions were 6 min at 94 °C, followed by 39–50 cycles of 40 s at each 94 °C, 56 °C, 72 °C, and a final elongation at 72 °C for 5 min.

Spiking PCR was performed in a final volume of 20 μl by adding 1 μl of spike control to 1.2X DreamTaq Buffer (including 2.4 mM MgCl2), 1 U DreamTaq Polymerase, 0.4 μg/μl BSA, 0.2 mM dNTP mix, 0.1–0.4 μM each primer, and 5 μl of template DNA. Thermal conditions were 2 min at 94 °C, followed by 33 cycles of 30 s at each 94 °C, 56 °C and 72 °C, and a final elongation at 72 °C for 10 min.

Real-time qPCR was set up in a final volume of 20 μl using SYBR® Green I (Sigma Aldrich), 1.2x Dream Taq Buffer (including 2.4 mM MgCl2), 1.25 U DreamTaq Polymerase, 0.4 μg/ml BSA, 0.2 mM dNTP mix (each), 0.2 μM of each primer, and 2 μl of template DNA. Primers rbcl PMF1/ R1 were used for the historic palm leaf and papyrus. For material made of Broussonetia, Daphne or Edgeworthia primer pair rbcl_PAPF1/R1 was used. Cycling conditions were 94 °C for 2 min followed by 40 cycles for each 30 s at 94 °C, 56 °C and 72 °C. Primer pair Bact1369F (CGGTGAATACGTTCYCGG) and Prok1492R (GGWTACCTTGTTACGACTT) was chosen to amplify 123 bp of bacterial rRNA [77] with the same conditions as described above A melting curve analysis was performed to monitor for non-specific amplicons.

Sequencing and sequence analysis.

Prior to sequencing PCR products were purified by enzymatic digestion with 2 U Exonuclease I (Thermo Fisher Scientific) and 0.3 U FastAP Thermosensitive Alkaline Phosphatase (Thermo Fisher Scientific) according to manufacturer’s protocol. Sanger sequencing was carried out externally by GATC Biotech AG. Sequence data were analyzed using MEGA (v.7.0.21) [78].

Authentication.

DNA analysis was performed in a facility with no prior exposure to paper, palm, or papyrus material. DNA extraction, amplification and post-PCR processing were carried out in separate laboratories in order to prevent carry-over contamination. Workspace and laboratory equipment were wiped with soap and ethanol on a regular basis. PCR was set up in a laminar flow workstation with UVC device. To monitor possible contaminations of the reagents blank controls were processed in parallel during extraction and PCR. For non-destructive sampling eraser material and nylon membranes with no exposure to DNA specimens were co-analyzed.

The authenticity of reference sequences from D. bholua and E. gardneri was provided by DNA extraction and PCR amplification of different sample sources (leaf and bark). Sequences were authenticated by at least two extractions and at least three independent PCR amplifications.

The authenticity of sequence results generated with non-invasive DNA sampling techniques was tested by independent DNA sampling, extraction and PCR set up.

Data quantification & statistical analyses.

Each of the eleven extraction methods was performed with three different sample weight inputs resulting in six extractions per material per method. Details are given in Table 9.

thumbnail
Table 9. Scheme of DNA extraction comparisons and PCR amplification.

https://doi.org/10.1371/journal.pone.0198513.t009

Influence of incubation time on DNA yield was tested on all writing supports Non-destructive DNA sampling was performed twice on all modern unbleached paper sheets, papyrus and palm leaf. Influence of paper production on DNA preservation was examined by DNA extraction using different lysis buffers, resulting in eleven DNA extractions for each material except for the historic paper sheet. Due to limited material only two extractions were performed on this sample. Details on experimental set up are listed in Table 10. Standard deviations were calculated using the STEDV function in MS Excel (2017). Paired t tests were calculated using SPSS software 25.

thumbnail
Table 10. Scheme of paper production comparisons and PCR amplification.

https://doi.org/10.1371/journal.pone.0198513.t010

Light microscopy.

Palm leaf material was sectioned into 5 mm long segments and soaked in pure water for one week. Subsequently the segments were treated with 30% PEG for one week and then embedded in 100% PEG. 20 μm thick sections were cut with a microtome and stained with 1% safranin for light microscopy. Images of palm sections were made using a light microscope (Zeiss Axioscope 40) equipped with a digital camera (Zeiss AxioCam MRc). Structural analysis was performed using ZEN 2012 (Zeiss software blue edition service pack 2).

SEM.

Small sections of leaf tissue were cut with a razor blade. After drying, the samples were coated by carbon (BIO-RAD SEM Coating System) and examined in a scanning electron microscope (Hitachi S520, Japan).

Acknowledgments

We thank Agnieszka Helman-Wazny, Michael Friedrich, Ira Rabin, and Gandolf Ulbricht for providing sample material, Alexander Möllers for technical assistance, and Marina Creydt and David Schütz for helpful discussion.

References

  1. 1. Pangallo D, Chovanova K, Makova A. Identification of animal skin of historical parchments by polymerase chain reaction (PCR)-based methods. J Archaeol Sci. 2010;37(6):1202–6.
  2. 2. Fiddyment S, Holsinger B, Ruzzier C, Devine A, Binois A, Albarella U, et al. Animal origin of 13th-century uterine vellum revealed using noninvasive peptide fingerprinting. PNAS. 2015;112(49):15066–71. pmid:26598667
  3. 3. Teasdale MD, van Doorn NL, Fiddyment S, Webb CC, O’Connor T, Hofreiter M, et al. Paging through history: parchment as a reservoir of ancient DNA for next generation sequencing. Philosophical transactions of the Royal Society of London Series B, Biological sciences. 2015;370(1660):20130379. pmid:25487331
  4. 4. Poulakakis N, Tselikas A, Bitsakis I, Mylonas M, Lymberakis P. Ancient DNA and the genetic signature of ancient Greek manuscripts. J Archaeol Sci. 2007;34(5):675–80.
  5. 5. López-Montalvo E, Roldán C, Badal E, Murcia-Mascarós S, Villaverde V. Identification of plant cells in black pigments of prehistoric Spanish Levantine rock art by means of a multi-analytical approach. A new method for social identity materialization using chaîne opératoire. PLOS ONE. 2017;12(2):e0172225. pmid:28207835
  6. 6. Bicchieri M, Monti M, Piantanida G, Sodo A. Non-destructive spectroscopic investigation on historic Yemenite scriptorial fragments: evidence of different degradation and recipes for iron tannic inks. Anal Bioanal Chem. 2013;405(8):2713–21. pmid:23307133
  7. 7. Burger J, Hummel S, Herrmann B. Palaeogenetics and cultural heritage. Species determination and STR-genotyping from ancient DNA in art and artefacts. Thermochimica Acta. 2000;365(1–2):141–6.
  8. 8. Richardin P, Cuisance F, Buisson N, Asensi-Amoros V, Lavier C. AMS radiocarbon dating and scientific examination of high historical value manuscripts: Application to two Chinese manuscripts from Dunhuang. JCH. 2010;11(4):398–403.
  9. 9. Bügler JH, Buchner H, Dallmayer A. Age Determination of Ballpoint Pen Ink by Thermal Desorption and Gas Chromatography–Mass Spectrometry. J Forensic Sci. 2008;53(4):982–8. pmid:18503526
  10. 10. Možir A, Gonzalez L, Kralj Cigić I, Wess TJ, Rabin I, Hahn O, et al. A study of degradation of historic parchment using small-angle X-ray scattering, synchrotron-IR and multivariate data analysis. Anal Bioanal Chem. 2012;402(4):1559–66. pmid:21928080
  11. 11. Strlič M, Cséfalvayová L, Kolar J, Menart E, Kosek J, Barry C, et al. Non-destructive characterisation of iron gall ink drawings: Not such a galling problem. Talanta. 2010;81(1):412–7. pmid:20188939
  12. 12. Marota I, Basile C, Ubaldi M, Rollo F. DNA decay rate in papyri and human remains from Egyptian archaeological sites. Am J Phys Anthropol. 2002;117(4):310–8. pmid:11920366
  13. 13. Dalén L, Lagerholm VK, Nylander JAA, Barton N, Bochenski ZM, Tomek T, et al. Identifying Bird Remains Using Ancient DNA Barcoding. Genes. 2017;8(6). pmid:28635635
  14. 14. Glocke I, Meyer M. Extending the spectrum of DNA sequences retrieved from ancient bones and teeth. Genome Res. 2017;27(7):1230–7. pmid:28408382
  15. 15. Oskam CL, Haile J, McLay E, Rigby P, Allentoft ME, Olsen ME, et al. Fossil avian eggshell preserves ancient DNA. Proc R Soc B. 2010. pmid:20219731
  16. 16. Liepelt S, Sperisen C, Deguilloux MF, Petit RJ, Kissling R, Spencer M, et al. Authenticated DNA from Ancient Wood Remains. Ann Bot. 2006;98(5):1107–11. pmid:16987920
  17. 17. Deguilloux MF, Pemonge MH, Petit RJ. Novel perspectives in wood certification and forensics: dry wood as a source of DNA. Proc Biol Sci. 2002;269(1495):1039–46. pmid:12028761
  18. 18. Schelm S, Haase I, Fischer C, Fischer M. Development of a Multiplex Real-Time PCR for Determination of Apricot in Marzipan Using the Plexor System. J Agric Food Chem. 2017;65(2):516–22. pmid:27943676
  19. 19. Di Bernardo G, Del Gaudio S, Galderisi U, Cascino A, Cipollaro M. Comparative evaluation of different DNA extraction procedures from food samples. Biotechnol Prog. 2007;23(2):297–301. pmid:17286386
  20. 20. Moncada X, Payacán C, Arriaza F, Lobos S, Seelenfreund D, Seelenfreund A. DNA Extraction and Amplification from Contemporary Polynesian Bark-Cloth. PLOS ONE. 2013;8(2):e56549. pmid:23437166
  21. 21. Vuissoz A, Worobey M, Odegaard N, Bunce M, Machado CA, Lynnerup N, et al. The survival of PCR-amplifiable DNA in cow leather. J Archaeol Sci. 2007;34(5):823–9.
  22. 22. O’Sullivan N, Teasdale M, Mattiangeli V, Maixner F, Pinhasi R, Bradley D, et al. A whole mitochondria analysis of the Tyrolean Iceman’s leather provides insights into the animal sources of Copper Age clothing. Sci Rep. 2016;6:31279. pmid:27537861
  23. 23. Bower MA, Campana MG, Checkley‐Scott C, Knight B, Howe C. The potential for extraction and exploitation of DNA from parchment: A review of the opportunities and hurdles. Journal of the Institute of Conservation. 2010;33(1):1–11.
  24. 24. Weiss CL, Schuenemann VJ, Devos J, Shirsekar G, Reiter E, Gould BA, et al. Temporal patterns of damage and decay kinetics of DNA retrieved from plant herbarium specimens. Royal Society open science. 2016;3(6):160239. pmid:27429780
  25. 25. Hansen HB, Damgaard PB, Margaryan A, Stenderup J, Lynnerup N, Willerslev E, et al. Comparing Ancient DNA Preservation in Petrous Bone and Tooth Cementum. PLOS ONE. 2017;12(1):e0170940. pmid:28129388
  26. 26. Gilbert MTP, Wilson AS, Bunce M, Hansen AJ, Willerslev E, Shapiro B, et al. Ancient mitochondrial DNA from hair. Curr Biol. 2004;14(12):R463–R4. pmid:15203015
  27. 27. Allentoft ME, Collins M, Harker D, Haile J, Oskam CL, Hale ML, et al. The half-life of DNA in bone: measuring decay kinetics in 158 dated fossils. Proc R Soc B. 2012. pmid:23055061
  28. 28. Kistler L, Ware R, Smith O, Collins M, Allaby RG. A new model for ancient DNA decay based on paleogenomic meta-analysis. Nucleic Acids Res. 2017;45(11):6310–20. pmid:28486705
  29. 29. Leach B. Papyrus Manufacture. In: Wendrich W, Dieleman J, Frood E, Aines J, editors. UCLA Encyclopedia of Egyptology. Los Angeles: University of California; 2009. p. 1–5.
  30. 30. Hunter D. Papermaking: The history and technique of an ancient craft. New York: Dover Publications; 1978.
  31. 31. Shepherd L. A non-destructive DNA sampling technique for herbarium specimens. PLOS ONE. 2017;12(8):e0183555. pmid:28859137
  32. 32. Särkinen T, Staats M, Richardson JE, Cowan RS, Bakker FT. How to Open the Treasure Chest? Optimising DNA Extraction from Herbarium Specimens. PLOS ONE. 2012;7(8):e43808. pmid:22952770
  33. 33. Rohland N, Siedel H, Hofreiter M. Nondestructive DNA extraction method for mitochondrial DNA analyses of museum specimens. BioTechniques. 2004;36(5):814–21. pmid:15152601
  34. 34. Thomsen PF, Elias S, Gilbert MTP, Haile J, Munch K, Kuzmina S, et al. Non-Destructive Sampling of Ancient Insect DNA. PLOS ONE. 2009;4(4). pmid:19337382
  35. 35. Castalanelli M, Severtson D, Brumley C, Szito A, Foottit R G., Grimm M, et al. A rapid non-destructive DNA extraction method for insects and other arthropods. J Asia-Pacif Entomol. 2010;13(2):243–8.
  36. 36. Hansson MC, Foley BP. Ancient DNA fragments inside Classical Greek amphoras reveal cargo of 2400-year-old shipwreck. J Archaeol Sci. 2008;35(5):1169–76.
  37. 37. Teasdale MD, Fiddyment S, Vnouček J, Mattiangeli V, Speller C, Binois A, et al. The York Gospels: a 1000-year biological palimpsest. R Soc Open Sci. 2017;4(10). pmid:29134095
  38. 38. Piñar G, Tafer H, Sterflinger K, Pinzari F. Amid the possible causes of a very famous foxing: molecular and microscopic insight into Leonardo da Vinci’s self‐portrait. Environ Microbiol Rep. 2015;7(6):849–59. pmid:26111623
  39. 39. Principi P, Villa F, Sorlini C, Cappitelli F. Molecular Studies of Microbial Community Structure on Stained Pages of Leonardo da Vinci’s Atlantic Codex. Microb Ecol. 2011;61(1):214–22. pmid:20811884
  40. 40. Puškárová A, Bučková M, Habalová B, Kraková L, Maková A, Pangallo D. Microbial communities affecting albumen photography heritage: a methodological survey. Sci Rep. 2016;6:20810. pmid:26864429
  41. 41. Hu Y, Fromm J, Schmidhalter U. Effect of salinity on tissue architecture in expanding wheat leaves. Planta. 2005;220(6):838–48. pmid:15503127
  42. 42. Fromm J, Hajirezaei M, Wilke I. The Biochemical Response of Electrical Signaling in the Reproductive System of Hibiscus Plants. Plant Physiol. 1995;109(2):375–84. pmid:12228601
  43. 43. Fromm J, Rockel B, Lautner S, Windeisen E, Wanner G. Lignin distribution in wood cell walls determined by TEM and backscattered SEM techniques. J Struct Biol. 2003;143:77–84. pmid:12892728
  44. 44. Langer K, Levchenko V, Fromm J, Geiger D, Steinmeyer R, Lautner S, et al. The poplar K+ channel KPT1 is associated with K+ uptake during stomatal opening and bud development. Plant J. 2004;37(6):828–38. pmid:14996212
  45. 45. Helman-Wazny A. Overview of Tibetan Paper and Papermaking: History, Raw Materials, Techniques and Fibre Analysis. In: Almogi O, editor. Tibetan Manuscripts and Xylograph Traditions. Hamburg: Department of Indian and Tibetan Studies, University of Hamburg; 2016. p. 171–96.
  46. 46. Nguyen-Hieu T, Aboudharam G, Drancourt M. Heat degradation of eukaryotic and bacterial DNA: an experimental model for paleomicrobiology. BMC Res Notes. 2012;5(1):528. pmid:23009640
  47. 47. Stone JE, Scallan AM. Effect of component removal upon the porous structure of the cell wall of wood. Journal of Polymer Science Part C: Polymer Symposia. 1965;11(1):13–25.
  48. 48. Reid I. Biodegradation of lignin. Can J Bot. 2011;73:1011–8.
  49. 49. Smith AH, Gill WM, Pinkard EA, Mohammed CL. Anatomical and histochemical defence responses induced in juvenile leaves of Eucalyptus globulus and Eucalyptus nitens by Mycosphaerella infection. For Pathol. 2007;37(6):361–73.
  50. 50. Bonello P, Storer AJ, Gordon TR, Wood DL, Heller W. Systemic Effects of Heterobasidion annosum on Ferulic Acid Glucoside and Lignin of Presymptomatic Ponderosa Pine Phloem, and Potential Effects on Bark-Beetle-Associated Fungi. J Chem Ecol. 2003;29(5):1167–82. pmid:12857029
  51. 51. Dumolin-Lapegue S, Pemonge MH, Gielly L, Taberlet P, Petit RJ. Amplification of oak DNA from ancient and modern wood. Mol Ecol. 1999;8(12):2137–40. pmid:10632865
  52. 52. Pollmann B, Jacomet S, Schlumbaum A. Morphological and genetic studies of waterlogged Prunus species from the Roman vicus Tasgetium (Eschenz, Switzerland). J Archaeol Sci. 2005;32(10):1471–80.
  53. 53. Mahmoudi Nasab H, Mardi M, Talaee H, Fazeli H, Pirseyedi S, Hejabri Nobari A, et al. Molecular Analysis of Ancient DNA Extracted from 3250–3450 Year-old Plant Seeds Excavated from Tepe Sagz Abad in Iran. Journal of Agricultural Science and Technology. 2010;12:459–70.
  54. 54. Rasmussen M, Guo X, Wang Y, Lohmueller KE, Rasmussen S, Albrechtsen A, et al. An Aboriginal Australian Genome Reveals Separate Human Dispersals into Asia. Science. 2011;334(6052):94–8. pmid:21940856
  55. 55. Staats M, Cuenca A, Richardson JE, Vrielink-van Ginkel R, Petersen G, Seberg O, et al. DNA Damage in Plant Herbarium Tissue. PLOS ONE. 2011;6(12):e28448. pmid:22163018
  56. 56. Angeles JGC, Laurena AC, Tecson-Mendoza EM. Extraction of genomic DNA from the lipid-, polysaccharide-, and polyphenol-rich coconut (Cocos nucifera L.). Plant Molecular Biology Reporter. 2005;23(3):297–8.
  57. 57. Simbolo M, Gottardi M, Corbo V, Fassan M, Mafficini A, Malpeli G, et al. DNA Qualification Workflow for Next Generation Sequencing of Histopathological Samples. PLOS ONE. 2013;8(6):e62692. pmid:23762227
  58. 58. Sedlackova T, Repiska G, Celec P, Szemes T, Minarik G. Fragmentation of DNA affects the accuracy of the DNA quantitation by the commonly used methods. Biological Procedures Online. 2013;15(1):5. pmid:23406353
  59. 59. Schrader C, Schielke A, Ellerbroek L, Johne R. PCR inhibitors—occurrence, properties and removal. J Appl Microbiol. 2012;113(5):1014–26. pmid:22747964
  60. 60. Huggett JF, Novak T, Garson JA, Green C, Morris-Jones SD, Miller RF, et al. Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon. BMC Res Notes. 2008;1:70-. pmid:18755023
  61. 61. Consolandi C, Palmieri L, Severgnini M, Maestri E, Marmiroli N, Agrimonti C, et al. A procedure for olive oil traceability and authenticity: DNA extraction, multiplex PCR and LDR–universal array analysis. Eur Food Res Technol. 2008;227(5):1429–38.
  62. 62. Cho W-B, Han E-K, Choi G, Lee J-H. The complete chloroplast genome of Daphne kiusiana, an evergreen broad-leaved shrub on Jeju Island. Conservation Genetics Resources. 2017.
  63. 63. van der Bank M, Fay M, Chase MW. Molecular Phylogenetics of Thymelaeaceae with Particular Reference to African and Australian Genera. Taxon. 2002;51(2):329–39.
  64. 64. Kistler L. Ancient DNA Extraction from Plants. In: Shapiro B, Hofreiter M, editors. Ancient DNA: Methods and Protocols. New York: Humana Press; 2012. p. 71–9.
  65. 65. Kalyankar VB, Ughade BR, Khedkar G, Gupta AK, Tiknaik A, Jamdade R, et al. Universal protocol for nucleic acid purification for plant taxa. Multilogic in Science An International Refreed & Indexed Quaterly Journal. 2012;2(III):11–4.
  66. 66. Ye J, Coulouris G, Zaretskaya I, Cutcutache I, Rozen S, Madden TL. Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction. BMC Bioinformatics. 2012;13(134). pmid:22708584
  67. 67. Sytsma KJ, Morawetz J, Pires JC, Nepokroeff M, Conti E, Zjhra M, et al. Urticalean rosids: circumscription, rosid ancestry, and phylogenetics based on rbcL, trnL-F, and ndhF sequences. Am J Bot. 2002;89(9):1531–46. pmid:21665755
  68. 68. Alverson W, Karol K, Baum D, Chase M, Swensen S, McCourt R, et al. Circumscription of the Malvales and relationships to other Rosidae: evidence from rbcL sequence data. Am J Bot. 1998;85(6):876. pmid:21684971
  69. 69. Schaefer H, Hardy OJ, Silva L, Barraclough TG, Savolainen V. Testing Darwin’s naturalization hypothesis in the Azores. Ecol Lett. 2011;14(4):389–96. pmid:21320262
  70. 70. Dong W, Xu C, Li C, Sun J, Zuo Y, Shi S, et al. ycf1, the most promising plastid DNA barcode of land plants. Sci Rep. 2015;5:8348. pmid:25672218
  71. 71. Sakulsathaporn A, Wonnapinij P, Vuttipongchaikij S, Apisitwanich S. The complete chloroplast genome sequence of Asian Palmyra palm (Borassus flabellifer). BMC Res Notes. 2017;10(1):740. pmid:29246263
  72. 72. Hahn W. A Molecular Phylogenetic Study of the Palmae (Arecaceae) Based on atp B, rbc L, and 18S nrDNA Sequences. Syst Biol. 2002;51(1):92–112. pmid:11943094
  73. 73. Asmussen CB, Chase MW. Coding and noncoding plastid DNA in palm systematics. Am J Bot. 2001;88(6):1103–17. pmid:11410476
  74. 74. Givnish TJ, Ames M, McNeal JR, McKain MR, Steele PR, dePamphilis CW, et al. Assembling the Tree of the Monocotyledons: Plastome Sequence Phylogeny and Evolution of Poales. Annals of the Missouri Botanical Garden. 2010;97(4):584–616.
  75. 75. Muasya AM, Simpson DA, Verboom GA, Goetghebeur P, Naczi RFC, Chase MW, et al. Phylogeny of Cyperaceae Based on DNA Sequence Data: Current Progress and Future Prospects. The Botanical Review. 2009;75(1):2–21.
  76. 76. Cornish-Bowden A. IUPAC-IUB Symbols for nucleotide nomenclature. Nucleic Acids Res. 1985;12:3021–30.
  77. 77. Suzuki MT, Taylor LT, DeLong EF. Quantitative Analysis of Small-Subunit rRNA Genes in Mixed Microbial Populations via 5′-Nuclease Assays. Appl Environ Microbiol. 2000;66(11):4605–14. pmid:11055900
  78. 78. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol. 2016;33(7):1870–4. pmid:27004904