Losing a fight against a conspecific male (social defeat) induces a period of suppressed aggressiveness and general behaviour, often with symptoms common to human psychiatric disorders. Agonistic experience is also discussed as a potential cause of consistent, behavioral differences between individuals (animal “personality”). In non-mammals, however, the impact of single agonistic encounters typically last only hours, but then again studies of repeated intermittent defeat (chronic social defeat) are seldom. We report the effect of chronic social defeat in adult male crickets (Gryllus bimaculatus), for which all known behavioral effects of defeat last only 3 h. Firstly, after 48 h social isolation, crickets that experienced 5 defeats at 24 h intervals against the same, weight-matched opponent exhibited suppressed aggressiveness lasting >24 h, which was still evident when the animals were matched against an unfamiliar opponent at the last trial. Secondly, this longer-term depression of aggression also occurred in 48 h isolated crickets that lost 6 fights at 1 h intervals against unfamiliar opponents at each trial. Thirdly, crickets isolated as larvae until adult maturity (>16 days) were significantly more aggressive, and less variable in their aggressiveness at their very first fight than 48 h isolates, and also significantly more resilient to the effects of chronic social defeat. We conclude that losing an aggressive encounter in crickets has a residual effect, lasting at least 24 h, that accumulates when repeated defeats are experienced, and leads to a prolonged depression of aggressive motivation in subordinates. Furthermore, our data indicate that social interactions between young adults and possibly larvae can have even longer, possibly lifelong influences on subsequent behavior. Social subjugation is thus likely to be a prime determinant of inter-individual behavioral differences in crickets. Our work also opens new avenues for investigating proximate mechanisms underlying depression-like phenomena.
Citation: Rose J, Rillich J, Stevenson PA (2017) Chronic social defeat induces long-term behavioral depression of aggressive motivation in an invertebrate model system. PLoS ONE 12(9): e0184121. https://doi.org/10.1371/journal.pone.0184121
Editor: Wolfgang Blenau, University of Cologne, GERMANY
Received: June 2, 2017; Accepted: August 18, 2017; Published: September 14, 2017
Copyright: © 2017 Rose et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was funded by the Deutsche Forschungsgemeinschaft, grant STE 714/4-1; J. Rillich is funded by grant RI 2728/2. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Social experience, particularly agonistic encounters between conspecifics, has profound effects on the subsequent behavior of all animals critically tested. Whereas winning a contest (social dominance) generally tends to enhance aggressiveness and the chances of winning subsequent encounters, losing (social defeat) is typically followed by a prolonged period of depressed aggressiveness and lowered chances of winning [1, 2]. Social defeat is also considered to be a major stressor , that in mammals induces depression like symptoms , involving reductions in locomotory behaviors and changes in brain chemicals, that become increasingly severe and persistent with repeated intermittent defeats (chronic social defeat, ). Investigations of the consequences of social defeat in a variety of animals are thus receiving increasing attention as potential models for understanding human psychiatric disorders such as depression and post-traumatic stress [6,7,8].
Although still under debate , evidence is also mounting that agonistic experience is both a cause and consequence of inter-individual differences in behavior, i.e. “personality”, in vertebrate and invertebrate animals alike (review: ). For example, tadpoles reared in isolation and without predatory cues no longer show inter-individual behavioral differences , while changes in dominance status erodes putative personality traits of exploration and activity in crickets (Teleogryllus oceanicus, ). Our recent work on male crickets (Gryllus bimaculatus) has also revealed significant differences in locomotory and exploratory behavior between aggressive winners and submissive losers of previous contests . Interestingly, these behavioral differences were also largely evident even before the contest, in individuals that were previously socially isolated for 48 h. This is surprising since all known behavioral effects of previous agonistic experience in crickets should have abated after this period of isolation: winners exhibit hyper-aggressiveness for no longer than 20 min after victory , while submissive losers typically regain their aggressiveness 0.5–3 hours after defeat [15–17]. However, since agonistic interactions can influence neurogenesis and gene expression in the house cricket Acheta domesticus [18, 19], longer term behavioral effects of social experience in crickets may have escaped the attention of previous investigators.
In this paper, we test the hypothesis that chronic social defeat may have longer term effects on the aggressive behavior of crickets than single defeats, as demonstrated in mammals [5, 7]. Three defeats were previously noted to extend the loser effect in crickets from 3 to 6 hours , but the phenomenon has not been studied systematically, and it is not known if more defeats have a greater effect. In dyadic contests between male fruit flies, the loser effect could be extended from several hours after a single defeat to a day after multiple defeats against the same opponent . In this study, lengthening of the duration of the loser effect was thought to partly result from losers adapting their fighting strategy towards familiar opponents, as well as changes to a more effective fighting strategy in winners. In the present study, we sought to eliminate potential effects of individual recognition and changing winner strategies, for example by swapping opponents at successive loser trials. Our data reveal that chronic social defeat alone can lead to longer term depression of aggressive motivation, lasting at least 24 hours and that social interactions between crickets in grouped colonies may even have the potential to forge lifelong differences in individual behavioral “personalities”.
Male Mediterranean field crickets, Gryllus bimaculatus (de Geer), were obtained from a commercial breeding stock (HW Terra, Aurachtal, Germany). All experiments were performed at ambient room temperature (22–25°C) and comply with animal welfare regulations in Germany. To minimize random differences in behavior due to daily variations in performance we always performed test and control experiments in parallel, avoiding times when aggression tends to be depressed (early afternoon and on generally dreary days, see [21, 22]). Our analysis is based on data gathered from interactions between 1576 pairs of male crickets. We defined basically 3 different groups depending on their experience prior to testing:
Short-term isolates (STI).
These crickets were obtained as young adults and first kept in our animal housing facility for 4–6 days as groups of 18–22 individuals under standard conditions (22–24°C, relative humidity 40–60%, 12–12 h light-dark regime daily feeding on bran and fresh vegetables, see ). Animals from this stock were then taken and kept isolated in individual glass jars with ample food for 48 h prior to experimentation.
Long-term isolates (LTI).
These crickets were obtained as last and last but one instar larvae and immediately isolated in individual glass jars, under the same conditions as STIs, for 6–8 days as larvae and for a further 10–14 days after the final moult as adults. They were thus kept in isolation for at least 16 to maximally 22 days.
These crickets served as standard opponents, against which the aggressiveness of STI and LTI crickets were evaluated. They were selected as noticeably larger individuals (> 5% heavier than test crickets) from the stock of STIs. Prior to testing, they were induced to fly for 3 min by suspending them in a warmed wind stream from a commercial grade air dryer, a treatment that markedly enhances aggression . Due to this treatment and their larger size they win practically all fights against test crickets; in the rare cases when not the data were not evaluated.
Evaluation of aggression and multiple defeat
Aggressive behavior was evaluated in dyadic contests (see ) in which the test crickets were either weight-matched (< 5% weight difference; data Fig 1, Table 1) or 5% lighter when matched against standard hyper-aggressive opponents (data in Figs 2 and 3, Tables 2 and 3). In each case the two opponents were placed at opposite ends of a small, Perspex-glass rectangular fighting arena (l. w. h.: 16 x 9 x 7 cm) having a sand-covered floor and divided halfway along its length by an opaque sliding door. On removing the door, the crickets generally interact with each other within seconds. Their interactions follow an escalating sequence of stereotyped motor performances [22, 25], which do not differ significantly from fights that occur in the field as part of their normal behavioral repertoire . The intensity of aggressiveness was scored on a scale of 0–6 [22, 24] denoting the level to which a fight escalates before the winner is established by the retreat of one contestant: Level 0: mutual avoidance without aggression. Level 1: one cricket attacks, the other retreats. Level 2: antennal fencing, whereby the contestants face each other and lash each other's antennae. Level 3: one contestant spreads its mandibles in a threat display. Level 4: mandible spreading by both crickets. Level 5: mandible engagement, whereby the two opponents interlock their mandibles. Level 6: grappling, an all-out fight involving repeated mandible engagements with biting, and body flipping. A contest can be concluded at any level by the retreat of one opponent. To check that a contest was indeed settled, we re-matched the same opponents < 3 min afterwards in a “loser-test”. In concluded fights, losers retreat immediately on contact, while the winner typically generates the characteristic rival song and body jerking movements . Fights that were not concluded were not evaluated. We also evaluated the relative frequency of immediate retreat (level 1) and physical fighting (levels 5 and 6) exhibited by the losers. Fight duration, from first contact until establishment of the winner and loser, was measured to the nearest second with a stopwatch. Very occasionally, the animals appeared to lose contact with each other so that fighting paused for a brief period before resuming when contact was regained. As in previous studies, we chose to deduct the duration of these pauses in the few cases in which they occur in order to give a more representative measure of the actual time spent fighting.
(A) The experimental paradigm. (Bi) Bar charts giving the level of aggression and (Ci) fight duration (circles M, bars IQR). (Di) Percentages of immediate retreats (level 1) and (Ei) physical fights (level 5 and 6). (Bii-Eii) As for Bi-Ei but for only half the previous losers that were re-matched against the same rivals (blue bars) and the remainder against different rivals (hatched blue bars). Significant differences between data sets as given by the Friedman test (FM) are indicated with asterisks, as are significant differences between the 1st and 5th fight as given by the Wilcoxon-signed rank test (WC) and chi-square test (CHI): *, ** P < 0.05, 0.01 respectively. N is giving above the x-axis in B.
(A) Experimental paradigm with legend. (Bi) Bar charts giving the level of aggression and (Ci) fight duration (circles M, bars IQR) for STI crickets that suffered multiple defeats against hyper-aggressive opponents (blue bars) compared to the performances of fresh STI crickets at each match that suffered no previous defeat (stippled bars). (Di) As in B1, but giving percentages of immediate retreats (level 1) and (Ei) physical fights (level 5 and 6). (Bii-Eii), as for Bi-Ei but 24 h and 48 h after the previous defeat. Significant differences between data sets as given by the Friedman test (FM) are indicated with asterisks, as are significant differences between individual bars as given by the Wilcoxon-signed rank test: *, **, *** P < 0.05, 0.01, 0.001 respectively. N is giving above the x-axis in B. Note the maintained depression of aggression 24 h after experiencing multiple defeats in the test group.
A) Pictogram of the experimental paradigm with legend. (Bi) Level of aggression and (Ci) fight duration (circles M, bars IQR) for STI and LTI crickets (blue bars, red bars respectively) matched repeatedly against hyper-aggressive opponents. (Di) As in B1, but giving percentages of immediate retreats (level 1) and (Di) physical fights l (level 5 and 6). (Aii-Dii) As for Bi-Ei, but for contests staged 24 h and 48 h after the last defeat. Significant differences between data sets as given by the Friedman test (FM) are indicated with asterisks, as are significant differences between individual bars as given by the Wilcoxon-signed rank test: *, **, *** P < 0.05, 0.01, 0.001 respectively. N is giving above the x-axis in B. Note the higher aggression of LTI crickets at the first fight and their earlier recovery 24 h after suffering multiple defeat.
The level and duration of aggression as well as percentages of immediate retreats and physical fights is given only for the 1st and 5th fights and also for a 6th fight staged 24 h later against either the same or an unfamiliar opponent (same opp., diff. opp.): 50%, 25–75%, 10–90% give the median, IQR and 10 and 90 percentiles respectively.
The level and duration of aggression as well as percentages of immediate retreats and physical fights is given for the 1st and 6th fights and also for a 7th fight staged either 24 h or 48 h later (recovery 24 h, 48 h): 50%, 25–75%, 10–90% give the median, IQR and 10 and 90 percentiles respectively.
The level and duration of aggression as well as percentages of immediate retreats and physical fights is given for the 1st and 6th fight and also for a 7th fight staged either 24 h or 48 h later (recovery 24 h, 48 h): 50%, 25–75%, 10–90% give the median, IQR and 10 and 90 percentiles respectively.
The effects of multiple defeats were investigated by re-matching test crickets repeatedly at either 1 h or 24 h intervals, ensuring that each contest was settled in a loser-test. In cases where the inter-fight interval was 24 h, test crickets were gently transferred back to their glass jars after each fight and supplied with ample moist food until the next trial. Previous studies have eliminated the possibility that gentle handling influences subsequent aggressive behavior of crickets .
All statistical tests were performed using standard commercial software (Prism 5, GraphPad Software Inc, La Jolla, CA, USA) running on a personal computer (Dell T3500, USA). The median (M) and the interquartile range (IQR) were calculated for all data sets. To test for significant differences in aggression with successive fights we applied the Friedman test for paired data sets and the Kruskal-Wallis test for unpaired data sets. Differences between specific groups with non-normally distributed data sets were tested using the Wilcoxon-signed rank test for paired data sets and the Mann-Whitney U test for unpaired data. The Chi-Square test was employed to test for differences in the frequency of immediate retreats and physical fights. Student’s unpaired t-test was used to test for differences in weight between multiple winners and losers as these data were normally distributed. The significance level was set at < 0.05 for comparing two groups.
Multiple defeat at 24 h intervals in short-term isolates (STI)
In our first experiment with STIs, we found that losers of an initial fight tended to lose again against the same previous opponents when matched 24 h later. Thus, from a group of 102 individual STI crickets that suffered their first defeat, the majority lost again at the next encounter against the same opponent 24 h later (76%, N = 78, data not illustrated). This trend continued so that at the 5th fight and after 4 previous defeats, 50% (N = 51) of the original cohort of 102 losers had lost 5 fights in succession against the same opponent. Since these persistent losers were not significantly different in weight to the persistent winners, fight outcome was not due to weight asymmetry (mean, standard deviation winners = 0.721, 0.099 g, losers: 0.731, 0.097 g; unpaired t-test: N = 51, T = 0.511, P = 0.609, not significant).
We next analyzed the aggressiveness of these 51 persistent losers at all their previous fights (Fig 1, Table 1 for statistical details). Corroborating earlier studies [22, 23, 25, 28, 29], the initial fights (0 previous defeats) of weight-matched male crickets after an isolation period of 24 h typically escalate to level 5 (median M, interquartile rang IQR = 5, 3–5; Fig 1Bi) and last several seconds (M, IQR = 7, 2–11 s; Fig 1Ci). Each of these fights generated clear losers which subsequently retreated from their previous opponents at the “loser-test” staged less than 3 min after defeat. As shown in earlier studies, the losers of a single previous fight engage again in physical fighting against the same opponents when tested at a 2nd fight 24 h later (level: M, IQR = 5, 2–5, duration: M, IQR = 5, 1–8 s). However, with each further defeat there was a clear reduction in aggression in terms of escalation level and fight duration (Friedman tests, FM: P level = 0.016, P duration < 0.001; Fig 1Bi and 1Ci). Thus, at the 5th fight the level of aggression and fight duration were both significantly lower than at the first fight (level: M, IQR = 3, 2–5, duration: M, IQR = 3, 1–7 s; level: W = 398, N = 51, P < 0.001; duration: W = 521, N = 51, P < 0.001). Furthermore, the relative frequency of immediate retreats exhibited by the losers on confronting the opponents increased from 12% at the 1st fight to 24% at the 5th, but this was not statistically significant (Chi-square test: X2 = 2.487, N = 51, P = 0.114; Fig 1Di, Table 1), whereas the frequency of actual physical interactions decreased significantly from 61% at the 1st fight, to 39% at the 5th fight (X2 = 4.936, N = 51, P = 0.026; Fig 1Ei).
To test whether the reduction in aggression of persistent losers is an adaptive strategy towards a familiar opponent, we staged an additional (6th) fight 24 h later, at which approximately half the losers of the 5th fight were re-matched against the same winners as in all previous fights, while the remainder were matched against a different winner. As illustrated in Fig 1Bii–1Eii, the persistent losers matched against the same winners were not less aggressive than those matched against different winners (Mann-Whitney U tests, level: U = 273, N1 = 25, N2 = 26, P = 0.315; duration: U = 292.5, P = 0.535; immediate retreats: X2 = 0.778, P = 0.079, physical fights: X2 = 1.543, P = 0.214). If anything, multiple losers matched against the familiar rival tended to be more aggressive, in that they escalated more often to physical fights, but this was not statistically significant.
Multiple defeat at 1 h intervals in short-term isolates (STI)
We next evaluated the effect of multiple defeats at shorter intervals (1 h). To completely eliminate the possibility of differences in aggression arising due to recognition of a familiar opponent, the test crickets were matched at each trial against a different “standard hyper-aggressive” opponent (flown for 3 min. prior to test; see [24, 30]). Each of these interactions were compared to those of fresh STIs, which experienced no previous defeats, matched at the same time against standard hyper-aggressive opponents (Fig 2, Table 2).
The test crickets, which suffered defeat at each successive fight showed a progressive reduction in aggression (same animals at each trial: FM, level: P < 0.001, duration: P < 0.001), whereas the control crickets, which were different fresh isolates at each trial, always exhibited physical fighting (level 5) with no difference in aggression at each trial (Kruskal-Wallis tests, KW, level: P = 0.132, duration: P = 0.522, Fig 2Bi and 2Ci, Table 2). Thus, test crickets at the 5th fight, that had experienced 4 previous defeats, rarely fought the hyper-aggressive opponent (level: M, IQR = 2, 1–3; duration: M, IQR = 1, 1–2 s; significantly different to controls: level: U = 358, N1 = 39, N2 = 86, P < 0.001; duration: U = 641, N1 = 39, N2 = 86, P < 0.001). This trend is also illustrated in Fig 2Di, which shows that the test crickets became increasingly more likely to retreat immediately on confronting the hyper-aggressive opponent with each successive defeat, and in Fig 2Ei which shows that the test crickets become progressively less likely to engage in physical interactions (immediate retreats: X2 = 23.397, P < 0.001; physical fights: X2 = 54.747, P < 0.001; Table 2).
Since crickets require at least 3 h to recover from a previous defeat [17, 23], it is not so surprising that they show depressed aggression after multiple defeats at 1h intervals. More relevant to this experiment, however, is the finding that multiple losers still showed depressed aggressiveness when tested 24 h after the last defeat (Fig 2Bii–2Eii). Compared to controls that suffered one defeat and fully recovered their aggressiveness after 24 h (level: M, IQR = 5, 3–5, duration: M, IQR = 4, 2–9 s), test crickets that suffered 6 previous defeats escalated less at the 7th fight (level: M, IQR = 3, 1–5; difference to control: U = 547.5, N1 = 45, N2 = 39, P = 0.002) and interacted significantly shorter periods of time (M, IQR = 4, 2–9 s: difference to controls: U = 629, N1 = 45, N2 = 39, P = 0.021; Fig 2Bii–2Eii). Furthermore, after the 24 h recovery time, the multiple losers exhibited immediate retreat more often than controls (test 33%, control 8%, X2 = 7.765, P = 0.005, Fig 2Dii), and less often physical fighting (test 31%, control crickets 56%, X2 = 5.339, P = 0.020; Fig 2Eii; Table 2). In contrast to this, multiple-defeated crickets given 48 h to recover from their last defeat appeared to have recovered their aggressiveness. For example, after 48 h recovery, neither the level nor duration of aggression was significantly different to control crickets (level: U = 789.5, N1 = 41, N2 = 39, P = 0.919, duration: U = 743.5, N1 = 41, N2 = 39, P = 0.589) and there were also no significant differences in the frequency of immediate retreat or physical interactions (immediate retreats: X2 = 0.714, P = 0.397; physical interactions: X2 = 0, P = 1).
Multiple defeat in long-term isolates (LTI)
Since a series of defeats at 1 h intervals can lead to longer term depression of aggression lasting at least 24 h (Fig 2), we next tested whether STIs may be influenced by earlier social experiences. To do this, we compared the aggressive behavior STIs to that of LTIs. As in the previous experiment, these crickets were also matched against a different, standard hyper-aggressive opponent at each contest (Fig 3, Table 3).
Interestingly, the LTIs were significantly more aggressive than the STIs at their very first fight (LTIs, level: M, IQR = 5, 5–5; duration: M, IQR = 6, 3–9 s; STIs level: M, IQR = 5, 3.25–5, duration: M, IQR = 3, 1–5 s; U level = 521, N1 = N2 = 40, P < 0.001, U duration = 467, P < 0.001; Fig 3Bi and 3Ci). This is also illustrated by the fact that none of the LTIs retreated on first confronting their hyper-aggressive opponents, compared to 13% immediate retreats for STIs (X2 = 5.561, P = 0.018, Fig 3Di). Furthermore, 85% of the LTIs engaged in physical fighting compared to 53% for STIs (X2 = 9.574, P = 0,001, Fig 3Ei).
After this first fight, the aggressiveness of both LTIs and STIs declined with each successive defeat (trials 2–6) and there were no longer any differences in the aggressive behavior between the two groups (FM: STI level: P < 0.001, duration: P < 0.001; LTI level: P < 0.001, duration: P < 0.001). Despite this, the LTIs appeared to be less susceptible to the effect of multiple defeat than STIs. Thus, 24 h after the last defeat, LTIs appeared to have partly regained their aggressiveness and were significantly more aggressive than the STIs with respect to their level of aggression (LTIs M, IQR = 5, 3–5; STIs M, IQR = 2, 1–4.5, U = 124, N1 = 21, N2 = 20, P = 0.020; Fig 3Bii) and frequency of aggressive interactions (immediate retreats: X2 = 2.334, P = 0.126, Fig 3Dii; physical interactions: X2 = 4.133, P = 0.042, Fig 3Eii). However, 48 h after the last defeat STIs and LTIs showed high levels of aggression, and there was no longer any difference between them (LTI level: M, IQR = 5, 4–5, duration: M, IQR = 4, 1.5–6 s; STI level: M, IQR = 5, 2–5, duration: M, IQR = 2, 1–4 s; U level = 183, N1 = 20, N2 = 19, P = 0.837, U duration = 123.5, N1 = 20, N2 = 19, P = 0.058; Fig 3Bii–3Eii, Table 3).
This study revealed that chronic social defeat has a long-term influence on the behavior of adult male crickets (Gryllus bimaculatus). As in many other animals [1, 2], crickets that have lost a fight behave submissively towards any adult conspecific male, and require at least 3 h to regain their original aggressive status [16, 17, 23, 24]. Whereas losers of a first fight are equally aggressive towards the previous winners after 24 h, we observed that the losers tended to lose again at this second fight and also at subsequent contests (for similar findings see  on Gryllus integer,  on Acheta domesticus and  on Drosophila). In addition, we found that losers that lost repeatedly against the same opponents on 5 consecutive days became progressively less aggressive with each defeat (Fig 1). There are several feasible explanations for these observations which must be considered before any conclusions can be drawn.
Relative body mass is known to be a major determinant of fighting success in many animals including crickets [21, 31, 32]. However, this does not explain why losers lost repeatedly, since they were not found to weigh less than their opponents. We also considered the possibility that the losers may have adjusted their fighting strategy towards familiar opponents, as suggested for Drosophila . When contestants recognise each other, aggression generally declines in subsequent interactions because relative dominance ranks have already been established [33, 34]. However, in species lacking individual recognition, aggression is not predicted to decline over subsequent encounters because the relative ranks of social partners are not clear unless individuals engage in new aggressive contests . Nonetheless, notwithstanding the fact that some insects have the capacity to discriminate individual conspecifics , this does not seem to apply to crickets. One-time losers are known to retreat even from unfamiliar opponents [15, 23, 26, 27, 37] and here we found that persistent losers re-matched against different rivals were equally submissive as those matched against familiar opponents (Fig 1).
Another explanation could be that reduced aggression in losers is due to some aspect of the winner’s behavior. In crayfish, for example, pheromone signals in the urine released by a dominant male acts on opponents to reduce the duration of future aggressive interactions . Male dominance also influences pheromone expression in the Australian field cricket Teleogryllus oceanicus . However, as far as presently known, cuticular pheromones in male crickets (see Tregenza, ) act rather to initiate [41, 42] and promote  aggression, rather than suppress it. Nonetheless, since winner crickets become more aggressive  and losers assess their opponent’s actions for the decision to flee , a winner’s augmented agonistic efforts could induce their opponents to give up earlier. However, winner-effects, which are widespread in the Animal Kingdom [16, 2], are presently not known to last longer than 20 min in crickets  and are thus unlikely to have influenced the losers when re-matched at 24 h intervals as in the present study.
Despite the above reasoning, and to strengthen our arguments, we sought to eliminate any potential influences of the winner-effect and individual recognition by evaluating the performances of test crickets that suffered multiple defeats at 1 h intervals against different hyper-aggressive opponents at each successive contest (Fig 2). Since the losers in this experiment were allowed less than the 3 h required by crickets to recover from social defeat [17, 23], it is perhaps not so surprising that their fighting efforts declined with each match. Of more significance was the finding that after 6 multiple defeats at 1 h intervals the losers exhibited a long-term depression of aggression that lasted longer than 24 h (Fig 2). Considering all arguments, the most parsimonious explanation for this is that losing has some residual effect, lasting at least 24 h, that will accumulate when repeated defeats are experienced within a given time frame, and that this leads to an extension of the loser effect, i.e. prolonged depression of aggressive motivation in subordinates. Previous workers have noted that three consecutive defeats can extend the duration of the loser effect in crickets for up to 6 h , but longer term loser-depression resulting from defeat and lasting over a whole day has never been observed.
In our present paradigms, the crickets appear to have recovered from chronic social defeat after 48 h. Despite this, our findings with long term isolated (LTI) crickets, which were kept individually from that last larval stages onwards until testing at sexual maturity, suggest that social interactions may have even longer, possibly lifelong, effects on subsequent behavior. For example, compared to short-term isolates (STIs, 48 h), LTIs (16–22 days) were significantly more aggressive at their very first fight as adults (Fig 3). None retreated on sighting the hyper-aggressive opponent, and the large majority (85%) engaged in physical fighting (levels 5 and 6). This is most unusual. To our knowledge, such homogeneity has never been observed in the aggressiveness of crickets, of which a significant proportion (10–20%) typically retreat immediately on contacting a conspecific male (see also Fig 1Di). Hence, crickets without any fighting experience as adults are highly aggressive and less variable in their fighting behavior. Furthermore, the LTIs were far less susceptible to multiple defeats, in that they were more aggressive than STIs 24 hours after 6 successive defeats. Numerous studies have noted that individuals reared in isolation have higher aggression [1, 15, 44] and isolation is often viewed as a pathological condition that can lead to increased aggressiveness . Experiments in crickets, however, have demonstrated that isolation allows the animals to escape from social subjugation by a few dominant individuals in a group and recover to a state of heightened aggressiveness, which in crickets is considered to be the natural default condition . Accordingly, LTIs are more aggressive and less prone to the effects of social defeat because they have had practically no previous adverse social agonistic experience as adults. It should also be noted, that larval crickets also engage in low level agonistic interactions , but it is not known whether they form stable dominant-subordinate relationships with possible consequence for the future behavior of adults.
Social experiences gained under grouped conditions typical for cricket breeding colonies will probably not only affect aggressive behavior. The experiences of losing 2 consecutive fights in short-term isolates (48 h) is known to result in reduced general motility and exploratory behavior, whereas winning has the opposite effects . In this earlier study, similar behavioral differences were also evident between the future winners and future losers 24 h before the fights were staged, indicating that earlier social experience could shape inter-individual behavioral differences in crickets (see also  on Gryllus integer). Our current and earlier study thus support the notion of “personality” in invertebrates being both a cause and consequence of contest behavior .
In mammals, including humans, social defeat, is generally regarded as a major stressor and risk factor for neuropsychiatric disorders such as depression [6–8]. In rodents, for example, repeated intermittent social defeat leads to reduced locomotor activity and elevated blood levels of stress hormones (cortisol and norepinephrine), but lower brain levels of the amines dopamine and serotonin . In insects, the biogenic amine octopamine, the invertebrate analogue of noradrenaline , is considered to be a major stress hormone (reviews: [49, 50]), that is released in crickets during fighting behavior  and in response to repeated fight or flight stress, such as repeated exposure to a mock predator . Similar to the effects of social defeat on cricket motility , exposure to a mock predator also leads to reduced locomotor activity and increased shelter-seeking behavior, and these effects could be mimicked by octopamine treatment . However, as far as is known in crickets, octopamine appears to have a promoting, rather suppressing effect on aggression [14, 25, 43]. Clearly, future investigations must therefore address the role of neuromodulators for establishing long-term behavioral depression following chronic social defeat.
This work forms part of the doctoral thesis of J. Rose. We thank our colleagues Julia Balsam and Stefan Schöneich for comments on the manuscript.
- 1. Hsu Y, Earley RL, Wolf LL. Modulation of aggressive behaviour by fighting experience: mechanisms and contest outcomes. Biol Rev Camb Philos Soc. 2006;81(1):33–74. pmid:16460581
- 2. Rutte C, Taborsky M, Brinkhof MW. What sets the odds of winning and losing? Trends Ecol Evol. 2006;21(1):16–21. pmid:16701465
- 3. Koolhaas JM, Meerlo P, de Boer SF, Strubbe JH, Bohus B. The temporal dynamics of the stress response. Neuroscience & Biobehavioral Reviews. 1997;21(6):775–82.
- 4. Carnevali L, Mastorci F, Graiani G, Razzoli M, Trombini M, Pico-Alfonso MA, et al. Social defeat and isolation induce clear signs of a depression-like state, but modest cardiac alterations in wild-type rats. Physiology & Behavior. 2012;106(2):142–50.
- 5. Yu WC, Liu CY, Lai WS. Repeated, intermittent social defeat across the entire juvenile period resulted in behavioral, physiological, hormonal, immunological, and neurochemical alterations in young adult male golden hamsters. Front Behav Neurosci. 2016;10:110. pmid:27375450
- 6. Huhman KL. Social conflict models: can they inform us about human psychopathology? Horm Behav. 2006;50(4):640–6. pmid:16870189
- 7. Hollis F, Kabbaj M. Social defeat as an animal model for depression. ILAR J. 2014;55(2):221–32. pmid:25225302
- 8. Sayed S, Iacoviello BM, Charney DS. Risk factors for the development of psychopathology following trauma. Curr Psychiatry Rep. 2015;17(10):80. pmid:26307035
- 9. Chichinadze K, Chichinadze N, Gachechiladze L, Lazarashvili A, Nikolaishvili M. Physical predictors, behavioural/emotional attributes and neurochemical determinants of dominant behaviour. Biol Rev Camb Philos Soc. 2014;89(4):1005–20. pmid:24533439
- 10. Briffa M, Sneddon LU, Wilson AJ. Animal personality as a cause and consequence of contest behaviour. Biol Lett. 2015;11(3).
- 11. Urszan TJ, Garamszegi LZ, Nagy G, Hettyey A, Torok J, Herczeg G. No personality without experience? A test on Rana dalmatina tadpoles. Ecol Evol. 2015;5(24):5847–56. pmid:26811759
- 12. Rudin FS, Tomkins JL, Simmons LW. Changes in dominance status erode personality and behavioral syndromes. Behavioral Ecology. 2016;28(1):270–9.
- 13. Rose J, Cullen DA, Simpson SJ, Stevenson PA. Born to win or bred to lose: aggressive and submissive behavioural profiles in crickets. Animal Behaviour. 2017;123:441–50.
- 14. Rillich J, Stevenson PA. Winning fights induces hyper-aggression via the action of the biogenic amine octopamine in crickets. PLoS ONE. 2011;6:e28891. pmid:22216137
- 15. Adamo SA, Hoy RR. Agonistic behavior in male and female field crickets, Gryllus bimaculatus, and how behavioural context influences its expression. Animal Behaviour. 1995;49(6):1491–501.
- 16. Iwasaki M, Delago A, Nishino H, Aonuma H. Effects of previous experience on the agonistic behaviour of male crickets, Gryllus bimaculatus. Zoological Science. 2006;23(10):863–72. pmid:17116989
- 17. Rillich J, Stevenson PA. A fighter's comeback: Dopamine is necessary for recovery of aggression after social defeat in crickets. Horm Behav. 2014;66(4):696–704. pmid:25268421
- 18. Ghosal K, Gupta M, Killian KA. Agonistic behavior enhances adult neurogenesis in male Acheta domesticus crickets. The Journal of Experimental Biology. 2009;212(Pt 13):2045–56. pmid:19525431
- 19. Ghosal K, Naples SP, Rabe AR, Killian KA. Agonistic behavior and electrical stimulation of the antennae induces Fos-like protein expression in the male cricket brain. Archives of Insect Biochemistry and Physiology. 2010;74(1):38–51. pmid:20422717
- 20. Trannoy S, Penn J, Luceya K, Popovicc D, Kravitz EA. Short and long-lasting behavioral consequences of agonistic encounters between male Drosophila melanogaster. Proceedings of the National Academy of Sciences. 2016;113:4818–23.
- 21. Dixon KA, Cade WH. Some factors influencing male-male aggression in the field cricket Gryllus-integer time of day age weight and sexual maturity. Animal Behaviour. 1986;34:340–6.
- 22. Stevenson PA, Hofmann HA, Schoch K, Schildberger K. The fight and flight responses of crickets depleted of biogenic amines. Journal of Neurobiology. 2000;43(2):107–20. pmid:10770840
- 23. Stevenson PA, Rillich J. Isolation associated aggression—a consequence of recovery from defeat in a territorial animal. PLoS One. 2013;8(9):e74965. pmid:24040368
- 24. Hofmann HA, Stevenson PA. Flight restores fight in crickets. Nature. 2000;403(6770):613–. pmid:10688185
- 25. Stevenson PA, Dyakonova V, Rillich J, Schildberger K. Octopamine and experience-dependent modulation of aggression in crickets. Journal of Neuroscience. 2005;25(6):1431–41. pmid:15703397
- 26. Alexander RD. Aggressiveness, territoriality, and sexual behaviour in field crickets (Orthoptera: Gyllidae). Behaviour. 1961;17:130–223.
- 27. Rillich J, Schildberger K, Stevenson PA. Assessment strategy of fighting crickets revealed by manipulating information exchange. Animal Behaviour. 2007;74(4):823–36.
- 28. Rillich J, Stevenson PA. Losing without fighting—Simple aversive stimulation induces submissiveness typical for social defeat via the action of nitric oxide, but only when preceded by an aggression priming stimulus. Frontiers in Behavioral Neuroscience. 2017;11(50):1–15.
- 29. Rillich J, Schildberger K, Stevenson PA. Octopamine and occupancy—an aminergic mechanism for intruder-resident aggression in crickets. Proceedings of the Royal Society London B. 2011;278:1873–80.
- 30. Stevenson PA, Rillich J. Adding up the odds—Nitric oxide signaling underlies the decision to flee and post-conflict depression of aggression. Science Advances 2015;1(2):e1500060. pmid:26601155
- 31. Hack MA. The energetic costs of fighting in the house cricket, Acheta domesticus L. Behavioral Ecology. 1997;8(1):28–36.
- 32. Simmons LW. Inter-male competition and mating success in the field cricket, Gryllus bimaculatus (de Geer). Animal Behaviour. 1986;34:567–79.
- 33. Dreier S, van Zweden JS, D'Ettorre P. Long-term memory of individual identity in ant queens. Biology Letters. 2007;3(5):459–62. pmid:17594958
- 34. Sheehan MJ, Tibbetts EA. Robust long-term social memories in a paper wasp. Current Biology. 2008;18(18):R851–2. pmid:18812075
- 35. Barnard CJ, Burk T. Dominance hierarchies and the evolution of individual recognition. Journal of Theoretical Biology. 1979;81(1):65–73. pmid:575180
- 36. Sheehan MJ, Tibbetts EA. Specialized face learning is associated with individual recognition in paper wasps. Science. 2011;334(6060):1272–5. pmid:22144625
- 37. Khazraie K, Campan M. The role of prior agonistic experience in dominance relationships in male crickets Gryllus bimaculatus (Orthoptera: Gryllidae). Behavioural Processes. 1999;44:341–8. pmid:24897236
- 38. Breithaupt T, Eger P. Urine makes the difference: chemical communication in fighting crayfish made visible. Journal of Experimental Biology. 2002;205(9):1221–31.
- 39. Thomas ML, Simmons LW. Male dominance influences pheromone expression, ejaculate quality, and fertilization success in the Australian field cricket, Teleogryllus oceanicus. Behavioral Ecology. 2009;20(5):1118–24.
- 40. Tregenza T, Wedell N. Definitive evidence for cuticular pheromones in a cricket. Animal Behaviour. 1997;54(4):979–84. pmid:9344449
- 41. Hofmann HA, Schildberger K. Assessment of strength and willingness to fight during aggressive encounters in crickets. Animal Behaviour. 2001;62(Part 2):337–48.
- 42. Iwasaki M, Katagiri C. Cuticular lipids and odors induce sex-specific behaviors in the male cricket Gryllus bimaculatus. Comparative Biochemistry and Physiology A. 2008;149(3):306–13.
- 43. Rillich J, Stevenson PA. Releasing stimuli and aggression in crickets: octopamine promotes escalation and maintenance but not initiation. Frontiers in Behavioral Neuroscience. 2015;9.
- 44. Koyama S. Isolation effect in mice (Mus-musculus). 1. Does it really induce aggression. Journal of Ethology. 1993;11(2):117–30.
- 45. Parmigiani S, Brain PF. Effects of residence, aggressive experience and intruder familiarity on attack shown by male mice. Behavioural Processes. 1983;8(1):45–57. pmid:24923607
- 46. Simmons LW. Competition between larvae of the field cricket, Gryllus bimaculatus (orthoptera: Gryllidae) and its effects on some life-history components of fitness. Journal of Animal Ecology. 1987;56:1015–27.
- 47. Kortet R, Hedrick A. A behavioural syndrome in the field cricket Gryllus integer: intrasexual aggression is correlated with activity in a novel environment. Biological Journal of the Linnean Society. 2007;91(3):475–82.
- 48. Pflüger H-J, Stevenson PA. Evolutionary aspects of octopaminergic systems with emphasis on arthropods. Arthropod Structure and Development. 2005;34(3):379–96.
- 49. Adamo SA. Norepinephrine and octopamine: linking stress and immune function across phyla. Invertebrate Survival Journal. 2008;5:12–9.
- 50. Verlinden H, Vleugels R, Marchal E, Badisco L, Pflüger HJ, Blenau W, et al. The role of octopamine in locusts and other arthropods. Journal of Insect Physiology. 2010;56(8):854–67. pmid:20621695
- 51. Adamo SA, Linn CE, Hoy RR. The role of neurohormonal octopamine during 'fight or flight' behaviour in the field cricket Gryllus bimaculatus. The Journal of Experimental Biology. 1995;198(Pt 8):1691–700. pmid:7636443
- 52. Adamo SA, Baker JL. Conserved features of chronic stress across phyla: the effects of long-term stress on behavior and the concentration of the neurohormone octopamine in the cricket, Gryllus texensis. Hormones and Behavior. 2011;60(5):478–83. pmid:21824475
- 53. Adamo SA, Kovalko I, Mosher B. The behavioural effects of predator-induced stress responses in the cricket (Gryllus texensis): the upside of the stress response. The Journal of Experimental Biology. 2013;216:4608–14. pmid:24307711