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Beyond HIV-serodiscordance: Partnership communication dynamics that affect engagement in safer conception care

  • Lynn T. Matthews ,

    Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Writing – original draft, Writing – review & editing

    ltmatthews@mgh.harvard.edu

    Affiliations Center for Global Health, Massachusetts General Hospital, Boston, MA, United States of America, Division of Infectious Disease, Massachusetts General Hospital, Boston, MA, United States of America

    ORCID http://orcid.org/0000-0001-6167-6328

  • Bridget F. Burns,

    Roles Data curation, Formal analysis, Writing – original draft, Writing – review & editing

    Affiliation Center for Global Health, Massachusetts General Hospital, Boston, MA, United States of America

  • Francis Bajunirwe,

    Roles Conceptualization, Funding acquisition, Investigation, Resources, Supervision, Writing – review & editing

    Affiliation Mbarara University of Science and Technology, Mbarara, Uganda

  • Jerome Kabakyenga,

    Roles Conceptualization, Funding acquisition, Investigation, Resources, Writing – review & editing

    Affiliation Mbarara University of Science and Technology, Mbarara, Uganda

  • Mwebesa Bwana,

    Roles Investigation, Resources, Writing – review & editing

    Affiliation Mbarara University of Science and Technology, Mbarara, Uganda

  • Courtney Ng,

    Roles Conceptualization, Formal analysis, Writing – review & editing

    Affiliation Harvard T.H. Chan School of Public Health, Boston, MA, United States of America

  • Jasmine Kastner,

    Roles Data curation, Formal analysis, Methodology, Project administration, Supervision, Writing – original draft, Writing – review & editing

    Affiliation Research Institute McGill University Health Centre, Montreal, Canada

  • Annet Kembabazi,

    Roles Conceptualization, Investigation, Project administration, Resources, Validation, Writing – review & editing

    Affiliation Mbarara University of Science and Technology, Mbarara, Uganda

  • Naomi Sanyu,

    Roles Formal analysis, Investigation, Writing – review & editing

    Affiliation Mbarara University of Science and Technology, Mbarara, Uganda

  • Adrine Kusasira,

    Roles Formal analysis, Investigation, Writing – review & editing

    Affiliation Mbarara University of Science and Technology, Mbarara, Uganda

  • Jessica E. Haberer,

    Roles Project administration, Resources, Writing – review & editing

    Affiliations Center for Global Health, Massachusetts General Hospital, Boston, MA, United States of America, Division of General Medicine, Massachusetts General Hospital, Boston, MA, United States of America

  • David R. Bangsberg,

    Roles Conceptualization, Funding acquisition, Investigation, Resources, Writing – review & editing

    Affiliation OHSU-PSU School of Public Health, Portland, OR, United States of America

  • Angela Kaida

    Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Project administration, Resources, Software, Supervision, Writing – original draft, Writing – review & editing

    Affiliation Faculty of Health Sciences, Simon Fraser University, Burnaby, BC, Canada

Beyond HIV-serodiscordance: Partnership communication dynamics that affect engagement in safer conception care

  • Lynn T. Matthews, 
  • Bridget F. Burns, 
  • Francis Bajunirwe, 
  • Jerome Kabakyenga, 
  • Mwebesa Bwana, 
  • Courtney Ng, 
  • Jasmine Kastner, 
  • Annet Kembabazi, 
  • Naomi Sanyu, 
  • Adrine Kusasira
PLOS
x

Abstract

Introduction

We explored acceptability and feasibility of safer conception methods among HIV-affected couples in Uganda.

Methods

We recruited HIV-positive men and women on antiretroviral therapy (ART) (‘index’) from the Uganda Antiretroviral Rural Treatment Outcomes cohort who reported an HIV-negative or unknown-serostatus partner (‘partner’), HIV-serostatus disclosure to partner, and personal or partner desire for a child within two years. We conducted in-depth interviews with 40 individuals from 20 couples, using a narrative approach with tailored images to assess acceptability of five safer conception strategies: ART for the infected partner, pre-exposure prophylaxis (PrEP) for the uninfected partner, condomless sex timed to peak fertility, manual insemination, and male circumcision. Translated and transcribed data were analyzed using thematic analysis.

Results

11/20 index participants were women, median age of 32.5 years, median of 2 living children, and 80% had HIV-RNA <400 copies/mL. Awareness of HIV prevention strategies beyond condoms and abstinence was limited and precluded opportunity to explore or validly assess acceptability or feasibility of safer conception methods. Four key partnership communication challenges emerged as primary barriers to engagement in safer conception care, including: (1) HIV-serostatus disclosure: Although disclosure was an inclusion criterion, partners commonly reported not knowing the index partner’s HIV status. Similarly, the partner’s HIV-serostatus, as reported by the index, was frequently inaccurate. (2) Childbearing intention: Many couples had divergent childbearing intentions and made incorrect assumptions about their partner’s desires. (3) HIV risk perception: Participants had disparate understandings of HIV transmission and disagreed on the acceptable level of HIV risk to meet reproductive goals. (4) Partnership commitment: Participants revealed significant discord in perceptions of partnership commitment. All four types of partnership miscommunication introduced constraints to autonomous reproductive decision-making, particularly for women. Such miscommunication was common, as only 2 of 20 partnerships in our sample were mutually-disclosed with agreement across all four communication themes.

Conclusions

Enthusiasm for safer conception programming is growing. Our findings highlight the importance of addressing gendered partnership communication regarding HIV disclosure, reproductive goals, acceptable HIV risk, and commitment, alongside technical safer conception advice. Failing to consider partnership dynamics across these domains risks limiting reach, uptake, adherence to, and retention in safer conception programming.

Introduction

Many men and women living with HIV and their partners want to have children [19]. As antiretroviral therapy (ART) becomes widely accessible, fertility desires and fertility of HIV-affected couples have increased, and are approaching rates observed among HIV unaffected couples [10, 11]. For those in HIV-serodiscordant partnerships, condomless sex to conceive introduces HIV transmission risk. While people living with HIV (PLWH), their partners, and providers are eager for safer conception programs [1216], HIV prevention during periconception receives relatively little attention [17, 18] [14, 1921].

Safer conception strategies include ART for the infected partner; delaying condomless sex until HIV-RNA suppression [22]; pre-exposure prophylaxis (PrEP) [2329]; treatment for sexually transmitted infections [30, 31]; sperm washing (for male-positive couples); and male circumcision and home insemination (for female-positive couples) [17, 32, 33]. Formative research has focused on acceptability (to consumers [12, 16, 19, 28]) and awareness (of providers [15, 3436]) of such methods. We conducted in-depth interviews in rural Uganda with PLWH and their uninfected or unknown-serostatus partners. The partner living with HIV reported that at least one member of the couple wanted a child in the next two years. Although our original research question focused on acceptability and feasibility of safer conception strategies, data revealed four domains of important partnership communication challenges to uptake of and engagement in safer conception services for HIV-affected couples.

Methods

Recruitment

Between 2005 and 2015, the Uganda Antiretroviral Rural Treatment Outcomes cohort (UARTO) recruited PLWH initiating ART in rural southwestern Uganda to determine predictors of ART adherence and virologic failure [37]. A Reproductive Health Component survey (2011–2015) assessed reproductive goals, and partnership dynamics, including partner’s HIV-serostatus and fertility desire [38]. Eligible index participants (“index”) for this sub-study were UARTO participants who completed the Reproductive Health Component, reported an HIV-negative or unknown-serostatus partner to whom they had disclosed their HIV status, and reported personal or partner pregnancy desire within two years. All study participants who completed an annual questionnaire between March 2013-April 2014 and who met the above criteria were approached by a research assistant to describe the linked qualitative study and determine interest in participating. After confirming interest and obtaining consent, index participants were asked to confirm HIV-serostatus disclosure to his/her partner, and then asked for permission for the study team to contact this partner. Partners (“partner” participants) were eligible for the sub-study after providing informed consent. Recruitment of index participants continued until data saturation was met.

Data collection

Socio-demographic information about index participants was obtained from the annual UARTO questionnaire preceding the in-depth qualitative interview. Similar data were obtained from partners through a brief, interviewer-administered questionnaire.

Individual in-depth interviews (conducted separately with each member of the couple) explored reproductive goals, partnership communication, concerns about periconception HIV transmission risks, and feasibility and acceptability of five safer conception strategies: ART for the infected partner, PrEP for the uninfected partner, timing of condomless sex to peak fertility, manual insemination, and male circumcision.

Prior to asking questions about awareness and acceptability, interviewers described safer conception strategies using case-based narratives and images (S1 Fig) [39]. Two co-authors (AK, FB) drafted the vignettes inspired by previous qualitative research findings [34] and clinical experience working with HIV-affected couples in Mbarara. The vignettes were then shared with five members of the Mbarara clinical and research team who evaluated the clarity, face validity, language, and appropriateness of the vignettes, including whether they reflected familiar situations and created a story to which participants would relate. Several revisions were suggested and incorporated through an iterative process to arrive at the final vignettes. In parallel, three co-authors (LTM, AK, JK, AK) worked with members of the research team and an artist to draft the images. Initial drafts were reviewed, discussed, and revised by the Mbarara clinical and research teams and members of the study site community advisory board over several weeks. The images were assessed for clarity of message and representativeness of the characters, including whether the images reflected familiar situations.

Interviews were conducted in the local language by a qualitative research assistant and audio-recorded. Each member of the couple was interviewed separately, rather than as a couple, in order to clearly assess the views of both the index and partner participant. We were specifically interested in understanding each individual’s considerations as he/she imagined making decisions about safer conception. While conducting couple interviews could have offered additional insights into safer conception decision-making, such an approach was beyond the scope of this study [40, 41].

Data analysis

Audio-recordings were transcribed and translated into English. Transcripts were coded independently by three researchers and analyzed using an iteratively developed codebook to explore emergent themes. Key data points were summarized, discussed, and compared for consistency and discrepancies to facilitate identification of connections among research questions, coding categories, and raw data. After the initial coding, discrepancies in HIV serostatus-disclosure, childbearing intentions, HIV risk acceptability, and understandings of the partnership commitment emerged from the data as barriers to choosing safer conception methods. These emergent findings were discussed with the full research and clinical team and identified as critical to informing the development of safer conception services for HIV-affected individuals and couples. Four members of the research team (including 2 of the primary coders) reviewed the transcripts and coding to classify the discrepancies across the 40 interviews with 20 dyads, select representative quotes, and summarizing the stories of two representative dyads. NVIVO software was used to organize the analysis. Quantitative socio-demographic data from the questionnaires are described using standard descriptive statistics.

Ethics statement

Ethics approvals were obtained from Mbarara University of Science and Technology (Uganda), Partners Healthcare (Boston, USA), and Simon Fraser University (Burnaby, Canada). Approvals were also obtained from the Uganda National Council for Science and Technology and the President’s Office. Participants provided written voluntary informed consent. Consistent with standard research participation reimbursements at this site, participants were given 1 bar of soap, 1kg of rice, or 1 kg of sugar for their participation and reimbursed for any associated transportation costs.

Results

Participant characteristics

We conducted in-depth interviews with 22 indexes and 20 partners. Data from two index participants whose partners were not interviewed are excluded. The 20 included index participants (Table 1) had a median age of 32.5 years and a median of two living children; 55% were women, and 85% reported an HIV-negative partner while 15% reported not knowing their partner’s HIV-serostatus. 75% reported personal desire for a child, and 85% reported that their partner desired a child. Indexes were on ART for a median of 2.8 years. Of those with viral load results, 80% (16/19) had HIV-RNA <400 copies/mL at the UARTO visit preceding the in-depth interview.

Partners had a median age of 34 years. 65% reported an HIV-negative serostatus while 35% reported an HIV-positive serostatus. 15% reported not knowing the index participant’s HIV status.

Overview of qualitative findings

Awareness of HIV prevention options other than condoms and abstinence was limited. All index partners were on effective antiretroviral treatment, but this was rarely identified as a strategy to decrease sexual transmission of HIV. Even with the narrative and image approach (S1 Fig) to explain safer conception methods, individuals’ awareness and understanding was insufficient for assessment of method acceptability or feasibility.

However, communication challenges within the dyad emerged as key barriers to uptake of and engagement in safer conception services. Partnership communication challenges occurred across four domains: (1) perception/knowledge of partner’s HIV-serostatus and disclosure, (2) childbearing intention, (3) HIV risk perception and acceptable level of HIV transmission risk, and (4) partnership commitment.

Illustrative case studies

To illustrate these partnership communication domains, we first present case studies from two participating couples, using pseudonyms (Table 2). The first couple, “Esther” and “Edward”, revealed concordant reports of HIV-serostatus disclosure/knowledge, childbearing goals, HIV transmission concerns, and understanding of the nature and future of the relationship. Only two couples in our sample of 20 (10%) presented such concordance.

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Table 2. Case studies (using pseudonyms) created from interviews with each member of the couple.

https://doi.org/10.1371/journal.pone.0183131.t002

The second couple described, “Janet” and “Julius”, is more typical of our sample, with interviews revealing substantial discordance across the four communication domains. In our sample, three couples presented discrepancy across all four domains (15%) while the remaining couples (15/20, 75%) presented discrepancies across 1 to 3 of the domains.

Partnership communication themes regarding childbearing in the context of HIV

Below we describe the four partnership communication themes that emerged from in-depth interviews with individual couple members, illustrated by participant quotes. Across themes, data reveal how gender norms influence HIV-affected couples’ communication and negotiation around childbearing.

Theme 1. HIV-serostatus disclosure/knowledge.

Although self report of HIV disclosure to partner was an index partner inclusion criterion, many partners reported not knowing the index’s HIV-serostatus. Similarly, the partner’s HIV-serostatus as reported by the index was frequently inaccurate, and mutual disclosure was uncommon. Different understandings of partnership HIV status manifested as the index and partner recounted different stories of how they informed or were informed about the partner’s HIV status.

Index: When I came to know [his status] is when he was telling me that we [should] have a child. [I said], ‘So to have a child, you see, for me, I am sick [HIV-positive], and you are ok [HIV-negative]…?’ [He said], ‘Can’t you see me having a healthy baby?’ [I said], ‘That doesn’t mean that if you are healthy and I am sick that we can’t have a baby who is not sick.’ … And he showed me the card [with his HIV-negative results]. Woman, Couple 11

Partner: … she told me she was sick. I also told her that I was sick. Male, Couple 11

Reasons for observed discrepancies in disclosure were rooted in gendered differences in disclosure experiences and risks. Male indexes commonly reported disclosing HIV status indirectly, often after the relationship was established:

Index: The drugs [ART], she came and found them in the locker. She found them there and she asked me ‘what are these drugs for’? I told her.

Interviewer: Before you married her, you had already started taking ARVs?

Index: Yes … Me, I don’t usually fear people. … by the time she saw the drugs, the thing [condomless sex] was already done. Male, Couple 3

In contrast, female index partners, reported struggling with disclosure, due to fears of violence, being “chased” from their homes, a loss of economic security, and having limited options for keeping and caring for a child.

Index: He kept quiet like for a week [after she disclosed her HIV-positive serostatus], we stayed in such a situation until I reached a time of producing our child, I told him, ‘Give me money, the medicine, I will not manage it.’ … He refused.–Woman, Couple 14

Theme 2. Childbearing intentions.

Participants commonly held divergent childbearing intentions and made incorrect assumptions about their partner’s desires. At times, the discordance in perceived childbearing intentions manifested as coercion. For instance, in this couple, the male index desires a child and expressed frustration with his partner’s reluctance to become pregnant. His partner reports attempting to dissuade him for her own health (she reports undergoing treatment for endometrial cancer) as well as the health of their older children; however, he is insistent.

Partner: He says ‘I want us to produce, I want another child such that they can be three.’ … I tell him that … I am not refusing to produce, I will produce, but let us first look after these ones and they grow a bit and then we [will] produce. … He says ‘You produce now.’ Woman, Couple 7

Across interviews, reports of direct communication within couples about childbearing intentions were rare, with narratives highlighting gendered expectations and assumptions about partner’s desires. The female index partner below is eager to have a child “for” her partner, whom she thinks is childless. He, however, has a child and is reluctant to have condomless sex to conceive and risk acquiring HIV:

Index: He was telling me that he wants a baby, because… he doesn’t have one. He says, ‘I want a child, … I want one child… I see that … your health is not good, I wouldn’t want to [make you have many children]. [He wants the child] in one year. Woman, Couple 9

Partner: I have one child but he is in Tanzania. […] The one child that I have is enough.

[….] When I saw that she was sick [HIV-positive] I decided to use a condom, since I saw that I could not chase her [end the relationship]. Male, Couple 9

Theme 3. HIV risk perceptions.

Confusion about HIV transmission risk to partners and infants was common, including disbelief that HIV-serodiscordance within an on-going sexual partnership was possible:

Index: … to find that a person is sick [HIV-positive], sleeps with someone who is not sick, and she doesn’t get sick, it also confuses me, how does it happen? …. You can be sick and you produce a healthy baby? …There is no way I can explain. Male, Couple 3

Within mutually disclosed partnerships, concerns about HIV transmission risk differed between individuals. Index participants expressed confidence that her/his partner was protected from HIV, either because of ART or through “God’s will” and “strong blood”. However, HIV-exposed but uninfected partners seldom shared this view. Women, in particular, feared acquiring HIV but felt they had few means to protect themselves:

Partner: “Condoms, he refused. … When we came from the hospital that very day when we tested, I told him now you see, you are sick. Me, I am not sick. Now let us use … the condom. … He said, he doesn't use a condom because he doesn't transmit the HIV virus.” Woman, Couple 7

Conversely, for the following couple, the female index is anxious about protecting her partner from acquiring HIV, whereas he is not worried because he believes he is protected.

Index: Now I ask myself, how can you stay with someone, you have the disease and it doesn't catch him? It confuses me, but my heart tells me that one day he will get it. Then what? Woman, Couple 12

Partner: How it happens [HIV-serodiscordance]? You may have blood that doesn’t easily catch the virus. Male, Couple 12

Disparate understandings of HIV transmission risk yielded disagreement regarding acceptable strategies to meet childbearing goals. For instance, HIV-positive men who desired children were reported to seek seroconcordant partners:

Partner: He said that … they tested him, … and found him with the virus, he knew that I can't stay with him anymore. He decided to look for another [woman], that's what he told me. I … told him, ‘Alright let me go [away] and you get a good family … [T]hen later when they failed each other, he came back. Woman, Couple 16

Women, however, described resigning themselves to the consequences of childbearing decisions made by the HIV-infected partner.

Partner: I told him that we should always have sexual intercourse using a condom; he swore and refused. … [H]e got the phone and called my mom that … I want to divorce.

Interviewer: So then have you ever had sex with a condom?

Partner: He refused [to use a condom]. Woman, Couple 3

Narratives revealed that HIV-negative women face pressures to bear children and secure their economic future that are often more compelling and urgent than avoiding HIV. This male index succinctly summarizes the issue as he describes his partner’s motivation to have his child:

Index: What worries her is not having HIV but having no property. Male, Couple 10

Theme 4. Relationship commitment.

All index participants reported personal or partner desire to have a child, and all partners consented to participate and verified a relationship with the index. Even so, within partnerships, individuals reported different reasons for and commitment to the relationship and, accordingly, different reasons for wanting to have (or not have) children. These discrepancies in understandings of partnership commitment compromised individuals’ ability to evaluate risks they would be willing to take to have a child. This female index participant describes her partner as a friend with whom she has no interest in having children. He describes her as a potential marriage partner and is eager to have a child with her, regardless of her HIV status.

Index: This one is my friend… I use him. … When he is around we are together. … When I tell him that, “I don’t want [to have your child]”, you see him feeling really bad. I say that, “even if you do want, for me I don’t want to have a baby. You go and look for those who will produce.” Woman, Couple 11

Partner: I went to her home and introduced and if God wishes it, we shall marry. Male, Couple 11

Discussion

In this study, we found limited awareness of HIV prevention strategies beyond condoms and abstinence, thus precluding analysis of participant acceptability or feasibility of the presented safer conception methods. Interviews revealed substantial and frequent discrepancies in perceptions and interpretations of partnership HIV-serostatus, childbearing intentions, acceptable HIV transmission risks, and relationship commitment. These areas of disagreement across the dyad made it challenging to interpret participant assessment of method preference. For instance, if a partner living with HIV considered certain HIV prevention methods as acceptable for her partner, but her partner subsequently reported that he was living with HIV, then the acceptability of the preferred method was less relevant. Or, for instance, if a partner living with HIV described his preferred safer conception method, but his partner did not want any (more) children, then a fulsome discussion of preferred methods seemed premature. The described partnership discrepancies are likely to present challenges to increasing linkage, uptake, and engagement of HIV affected individuals and couples in safer conception programming. The particular risks reported by women in terms of communicating serostatus, reproductive goals, and relationship security highlight the importance of centering gender-informed approaches within safer conception programming to maximize women’s reproductive autonomy and safety [42].

A large body of literature explores the complexities of sexual partnerships and how those relationships impact HIV risk reduction behaviours and inform interventions [4347]. The safer conception literature has not routinely explored these complexities and often assumes that safer conception services will be offered to mutually-disclosed HIV-serodiscordant couples with concordant fertility desires and attitudes towards risk [15, 34, 4854]. Our findings highlight the importance of considering the complexities of dyadic dynamics when developing or implementing safer conception programming.

Low rates of direct serostatus disclosure to sexual partners have been described, and qualitative studies document fears of abandonment, blame, and violence associated with disclosure [47, 5557]. In our study, index partners were only eligible for this study if they reported having disclosed to and knowing the partner’s status, yet we identified numerous instances whereby disclosure was incomplete. There are several possible explanations for these findings, including that index partners may disclose using non-verbal clues (e.g., leaving medications and/or HIV test results in plain view, alluding to serostatus), whereas partners may not have deduced the index’s status from these clues [56]. There likely exists a social desirability bias to report serostatus disclosure to the partner [58]. And finally, there may have been motivation to participate in the study in order to receive transport refund and other benefits [59]. Collectively, our data highlight the complexity and poor reliability of self-report of disclosure and the importance of addressing supported disclosure within safer conception programs.

An analysis of reproductive goals among HIV-serodiscordant couples in the Partners PrEP study revealed that men’s reproductive goals and practices often dominate [60], concurring with other studies [5, 7, 8, 61, 62]. Our study corroborates this while also describing partnership discordance in reproductive goals, a lack of communication regarding such goals, and assumptions about a partner’s reproductive goals based on gendered social norms. These findings underscore the importance of and challenges to offering both individual and couples-based safer conception services.

We also observed discrepancies in the level of HIV risk that partners were willing to assume to conceive a child–in part due to differential understandings of transmission risk. Disbelief that serodiscordance is possible negatively affected appreciation of the potential of safer conception practices–i.e., HIV-serodiscordance is simply not believed and the uninfected individual avoids testing, assuming that transmission has already occurred. Others believe that remaining uninfected while a partner is infected is due to factors that do not require intervention (e.g., “strong blood”). Again, given prevailing gender norms, regardless of who is infected, the male partner yields substantial power in determining if, when, and how risk reduction practices will be deployed, highlighting the importance of engaging men within safer conception programming [43, 6368].

Effective support for and decisions about disclosure, reproductive goals, and HIV-prevention methods depend on understanding relationship dynamics and trade-offs made to initiate, sustain, and nurture partnerships. Encouraging communication about relationship and childbearing goals and plans must be considered a foundational component of supporting couples to choose and use safer conception methods [42, 6971].

The communication gaps across the four described domains highlight an important opportunity for couples-based HIV counseling and testing (CHCT) in safer conception programming [24]. CHCT facilitates mutual HIV-serostatus disclosure, discussion of fertility intention, uptake of contraceptives when pregnancy is not desired, reductions in HIV and STI incidence [61, 7275] and thus presents a platform to discuss safer conception based on couples’ HIV status and childbearing desires. The Ugandan Ministry of Health includes couples-based HIV counselling and testing as a key strategy to improve HIV prevention and treatment efforts [76], although uptake remains low [77]. Interventions to improve CHCT services and promote CHCT uptake are urgently needed. Leveraging the desires of individuals and couples to have healthy children may be an effective strategy to promote CHCT such that more couples are able to benefit from this crucial service. We hypothesize that framing disclosure within a holistic approach that focuses on building healthy families may support couples to overcome barriers to disclosure, and testing of this hypothesis is ongoing [19, 68, 7880].

These data highlighting the importance and challenge of offering safer conception care to HIV-affected individuals or couples who are not able or willing to present with their partner. To be inclusive, counseling programs must offer services to individuals who may need options they can implement independent of a partner. For instance, in a safer conception program in South Africa, 100% of male index clients brought their female partners to the clinic, but only 56% of women brought their male partners, highlighting the known challenges that women face engaging their partners in reproductive health programming [81]. Studies in South Africa further highlight the complex relationship power dynamics that underlie communication, trust, decisions and risk behaviors [43, 44, 47]. The work reported here also reveals a gendered differential in safer reproductive decision-making.

Implementation of safer conception methods is most feasible with concordance between members of a couple across the four themes identified here. However, safer conception services might be best implemented in a harm reduction paradigm [54] wherein all HIV-affected persons (independent of partner participation and serostatus disclosure) are supported to make informed decisions about meeting their reproductive goals and CHCT is encouraged [24]. Qualitative studies highlight the dilemma providers face when offering safer conception advice to clients who are not yet able to disclose [82]. Developing strategies for providers to support individuals to approach safe disclosure and CHCT remains a priority [8385] [24] [82].

This study has several limitations. Data come from a small sample and are not meant to be generalized. However, interviewing occurred until we reached thematic saturation. As a qualitative study, findings should be interpreted as hypothesis-generating for future research, and need to be evaluated using quantitative methodologies with larger samples. Index participants were engaged in care, accessing ART, and reported HIV disclosure and are, thus, not representative of the larger community of HIV-affected couples needing safer conception services. Given persistent stigma towards childbearing among PLWH, social desirability bias may have affected the information shared in the interviews.

Conclusion

Awareness of the need for and potential of safer conception programming is growing. Our findings highlight that such programming must be prepared to address complex experiences of communication across multiple partnership domains, in addition to offering technical advice regarding safer conception strategies if we are to maximize reach of and engagement in safer conception programming to all HIV-affected individuals and couples requiring such services.

Supporting information

S1 Fig. Images and narratives for five safer conception methods.

The following images and narratives were used to share information with participants about the various safer conception strategies to reduce sexual transmission between HIV-serodiscordant couple while allowing for conception. Images accompanied each narrative. Images and narratives were developed in close consultation with the clinical and research teams based in Uganda. Questions embedded throughout the narratives to encourage discussion are removed from this supplemental information.

https://doi.org/10.1371/journal.pone.0183131.s001

(DOCX)

Acknowledgments

The authors would like to thank study participants and our research team for their contributions to this work.

Meetings at which preliminary versions of these data were presented: Preliminary data were presented in abstract form at the inaugural HIVR4P meeting (Cape Town, October 2014) and at the Mbarara University of Science and Technology (MUST)-Massachusetts General Hospital Inaugural Reproductive Health Conference (Mbarara, Uganda May 2016).

References

  1. 1. Kastner J, Matthews LT, Flavia N, Bajunirwe F, Erikson S, Berry NS, et al. Antiretroviral Therapy Helps HIV-Positive Women Navigate Social Expectations for and Clinical Recommendations against Childbearing in Uganda. AIDS research and treatment. 2014;2014:626120. pmid:25328693; PubMed Central PMCID: PMC4189848.
  2. 2. Guthrie BL, Choi RY, Bosire R, Kiarie JN, Mackelprang RD, Gatuguta A, et al. Predicting pregnancy in HIV-1-discordant couples. AIDS and behavior. 2010;14(5):1066–71. Epub 2010/06/15. pmid:20544384; PubMed Central PMCID: PMCPMC3051103.
  3. 3. Myer L, Morroni C, Rebe K. Prevalence and determinants of fertility intentions of HIV-infected women and men receiving antiretroviral therapy in South Africa. AIDS Patient Care STDS. 2007;21(4):278–85. 17461723. pmid:17461723
  4. 4. Brubaker SG, Bukusi EA, Odoyo J, Achando J, Okumu A, Cohen CR. Pregnancy and HIV transmission among HIV-discordant couples in a clinical trial in Kisumu, Kenya. HIV Med. 2011;12(5):316–21. Epub 2011/01/06. pmid:21205129.
  5. 5. Beyeza-Kashesya J, Ekstrom AM, Kaharuza F, Mirembe F, Neema S, Kulane A. My partner wants a child: a cross-sectional study of the determinants of the desire for children among mutually disclosed sero-discordant couples receiving care in Uganda. BMC public health. 2010;10:247. pmid:20465794; PubMed Central PMCID: PMCPMC2877675.
  6. 6. Homsy J, Bunnell R, Moore D, King R, Malamba S, Nakityo R, et al. Reproductive intentions and outcomes among women on antiretroviral therapy in rural Uganda: a prospective cohort study. PLoS ONE. 2009;4(1):e4149. pmid:19129911.
  7. 7. Nakayiwa S, Abang B, Packel L, Lifshay J, Purcell DW, King R, et al. Desire for children and pregnancy risk behavior among HIV-infected men and women in Uganda. AIDS and behavior. 2006;10(4 Suppl):S95–104. Epub 2006/05/23. pmid:16715343.
  8. 8. Awiti Ujiji O, Ekstrom AM, Ilako F, Indalo D, Rubenson B. "I will not let my HIV status stand in the way." Decisions on motherhood among women on ART in a slum in Kenya- a qualitative study. BMC Womens Health. 2010;10:13. Epub 2010/04/29. pmid:20423528; PubMed Central PMCID: PMC2873237.
  9. 9. Maier M, Andia I, Emenyonu N, Guzman D, Kaida A, Pepper L, et al. Antiretroviral Therapy is Associated with Increased Fertility Desire, but not Pregnancy or Live Birth, among HIV+ Women in an Early HIV Treatment Program in Rural Uganda. AIDS Behav. 2009;13 Suppl 1:28–37. pmid:18389364; PubMed Central PMCID: PMC18389364.
  10. 10. Matovu JK, Makumbi F, Wanyenze RK, Serwadda D. Determinants of fertility desire among married or cohabiting individuals in Rakai, Uganda: a cross-sectional study. Reproductive health. 2017;14(1):2. pmid:28069056; PubMed Central PMCID: PMCPMC5223449.
  11. 11. Marston M, Nakiyingi-Miiro J, Hosegood V, Lutalo T, Mtenga B, Zaba B, et al. Measuring the Impact of Antiretroviral Therapy Roll-Out on Population Level Fertility in Three African Countries. PLoS One. 2016;11(3):e0151877. pmid:27015522; PubMed Central PMCID: PMCPMC4807830.
  12. 12. Matthews LT, Moore L, Milford C, Greener R, Mosery FN, Rifkin R, et al. "If I don't use a condom… I would be stressed in my heart that I've done something wrong": Routine Prevention Messages Preclude Safer Conception Counseling for HIV-Infected Men and Women in South Africa. AIDS and behavior. 2015. pmid:25711300.
  13. 13. Kaida A, Kastner J, Ng C, Sanyu N, Kusasira A, Kabakyenga J, et al. Barriers and Promoters to Uptake of Safer Conception Strategies among HIV-serodiscordant Couples with Fertility Intention in Mbarara, Uganda. AIDS Research and Human Retroviruses. 2014;30(Supplement 1):A61–A2.
  14. 14. Wanyenze RK, Wagner GJ, Tumwesigye NM, Nannyonga M, Wabwire-Mangen F, Kamya MR. Fertility and contraceptive decision-making and support for HIV infected individuals: client and provider experiences and perceptions at two HIV clinics in Uganda. BMC public health. 2013;13:98. Epub 2013/02/05. pmid:23374175; PubMed Central PMCID: PMC3568663.
  15. 15. West N, Schwartz S, Phofa R, Yende N, Bassett J, Sanne I, et al. "I don't know if this is right… but this is what I'm offering": healthcare provider knowledge, practice, and attitudes towards safer conception for HIV-affected couples in the context of Southern African guidelines. AIDS care. 2016;28(3):390–6. pmid:26445035.
  16. 16. Schwartz SR, West N, Phofa R, Yende N, Sanne I, Bassett J, et al. Acceptability and preferences for safer conception HIV prevention strategies: a qualitative study. Int J STD AIDS. 2015. pmid:26384950.
  17. 17. Mmeje O, van der Poel S, Workneh M, Njoroge B, Bukusi E, Cohen CR. Achieving pregnancy safely: perspectives on timed vaginal insemination among HIV-serodiscordant couples and health-care providers in Kisumu, Kenya. AIDS care. 2015;27(1):10–6. Epub 2014/08/12. pmid:25105422; PubMed Central PMCID: PMC4221525.
  18. 18. Drake AL, Wagner A, Richardson B, John-Stewart G. Incident HIV during pregnancy and postpartum and risk of mother-to-child HIV transmission: a systematic review and meta-analysis. PLoS medicine. 2014;11(2):e1001608. Epub 2014/03/04. pmid:24586123; PubMed Central PMCID: PMC3934828.
  19. 19. Matthews LT, Crankshaw T, Giddy J, Kaida A, Smit JA, Ware NC, et al. Reproductive Decision-Making and Periconception Practices Among HIV-Positive Men and Women Attending HIV Services in Durban, South Africa. AIDS and behavior. 2013;17(2):461–70. Epub 2011/11/01. pmid:22038045; PubMed Central PMCID: PMCPMC3560938.
  20. 20. Dyer S, Mokoena N, Maritz J, van der Spuy Z. Motives for parenthood among couples attending a level 3 infertility clinic in the public health sector in South Africa. Hum Reprod. 2008;23(2):352–7. pmid:18063652.
  21. 21. Schwartz SR, Mehta SH, Taha TE, Rees HV, Venter F, Black V. High Pregnancy Intentions and Missed Opportunities for Patient-Provider Communication About Fertility in a South African Cohort of HIV-Positive Women on Antiretroviral Therapy. AIDS Behav. 2011. Epub 2011/06/10. pmid:21656145.
  22. 22. Rodger AJ, Cambiano V, Bruun T, Vernazza P, Collins S, van Lunzen J, et al. Sexual Activity Without Condoms and Risk of HIV Transmission in Serodifferent Couples When the HIV-Positive Partner Is Using Suppressive Antiretroviral Therapy. JAMA. 2016;316(2):171–81. pmid:27404185.
  23. 23. Cohen MS, Chen YQ, McCauley M, Gamble T, Hosseinipour MC, Kumarasamy N, et al. Prevention of HIV-1 Infection with Early Antiretroviral Therapy. N Engl J Med. 2011. Epub 2011/07/20. pmid:21767103.
  24. 24. World_Health_Organization. Guidance on couples HIV testing and counselling including antiretroviral therapy for treatment and prevention in serodiscordant couples. Geneva: 2012 April 2012. Report No.
  25. 25. Baeten JM, Donnell D, Ndase P, Mugo NR, Campbell JD, Wangisi J, et al. Antiretroviral prophylaxis for HIV prevention in heterosexual men and women. N Engl J Med. 2012;367(5):399–410. pmid:22784037
  26. 26. Thigpen MC, Kebaabetswe PM, Paxton LA, Smith DK, Rose CE, Segolodi TM, et al. Antiretroviral Preexposure Prophylaxis for Heterosexual HIV Transmission in Botswana. N Engl J Med. 2012;367(5):423–34. Epub 2012/07/13. pmid:22784038.
  27. 27. Vernazza PL, Graf I, Sonnenberg-Schwan U, Geit M, Meurer A. Preexposure prophylaxis and timed intercourse for HIV-discordant couples willing to conceive a child. Aids. 2011;25(16):2005–8. pmid:21716070
  28. 28. Whetham J, Taylor S, Charlwood L, Keith T, Howell R, McInnes C, et al. Pre-exposure prophylaxis for conception (PrEP-C) as a risk reduction strategy in HIV-positive men and HIV-negative women in the UK. AIDS Care. 2014;26(3):332–6. pmid:23876052.
  29. 29. Heffron R, Pintye J, Matthews LT, Weber S, Mugo N. PrEP as Peri-conception HIV Prevention for Women and Men. Curr HIV/AIDS Rep. 2016. pmid:26993627.
  30. 30. Grosskurth H, Mosha F, Todd J, Mwijarubi E, Klokke A, Senkoro K, et al. Impact of improved treatment of sexually transmitted diseases on HIV infection in rural Tanzania: randomised controlled trial. Lancet. 1995;346(8974):530–6. pmid:7658778.
  31. 31. Wawer MJ, Gray RH, Sewankambo NK, Serwadda D, Paxton L, Berkley S, et al. A randomized, community trial of intensive sexually transmitted disease control for AIDS prevention, Rakai, Uganda. Aids. 1998;12(10):1211–25. pmid:9677171.
  32. 32. Zafer M, Horvath H, Mmeje O, van der Poel S, Semprini AE, Rutherford G, et al. Effectiveness of semen washing to prevent human immunodeficiency virus (HIV) transmission and assist pregnancy in HIV-discordant couples: a systematic review and meta-analysis. Fertil Steril. 2016;105(3):645–55 e2. pmid:26688556; PubMed Central PMCID: PMCPMC4779710.
  33. 33. Siegfried N, Muller M, Deeks JJ, Volmink J. Male circumcision for prevention of heterosexual acquisition of HIV in men. Cochrane Database Syst Rev. 2009;(2):CD003362. pmid:19370585.
  34. 34. Matthews LT, Bajunirwe F, Kastner J, Sanyu N, Akatukwasa C, Ng C, et al. "I Always Worry about What Might Happen Ahead": Implementing Safer Conception Services in the Current Environment of Reproductive Counseling for HIV-Affected Men and Women in Uganda. Biomed Res Int. 2016;2016:4195762. pmid:27051664; PubMed Central PMCID: PMCPMC4802028.
  35. 35. Matthews LT, Moore L, Crankshaw TL, Milford C, Mosery FN, Greener R, et al. South Africans with recent pregnancy rarely know partner's HIV serostatus: implications for serodiscordant couples interventions. BMC Public Health. 2014;14:843–. pmid:25124267
  36. 36. Goggin K, Finocchario-Kessler S, Staggs V, Woldetsadik MA, Wanyenze RK, Beyeza-Kashesya J, et al. Attitudes, Knowledge, and Correlates of Self-Efficacy for the Provision of Safer Conception Counseling Among Ugandan HIV Providers. AIDS Patient Care STDS. 2015;29(12):651–60. pmid:26588429; PubMed Central PMCID: PMCPMC4684655.
  37. 37. Musinguzi N, Mocello RA, Boum Y 2nd, Hunt PW, Martin JN, Haberer JE, et al. Duration of Viral Suppression and Risk of Rebound Viremia with First-Line Antiretroviral Therapy in Rural Uganda. AIDS and behavior. 2016;21(6):1735–40. pmid:27256394; PubMed Central PMCID: PMCPMC5135647.
  38. 38. Ahluwalia IB, Johnson C, Rogers M, Melvin C. Pregnancy Risk Assessment Monitoring System (PRAMS): unintended pregnancy among women having a live birth. PRAMS Working Group. J Womens Health Gend Based Med. 1999;8(5):587–9. Epub 2000/06/06. pmid:10839641.
  39. 39. Norman GR, Schmidt HG. The psychological basis of problem-based learning: a review of the evidence. Acad Med. 1992;67(9):557–65. pmid:1520409.
  40. 40. Allan G. A note on interviewing spouses together. Journal of Marriage and Family. 1980;42(1):205–10.
  41. 41. Hertz R. Separate But Simultaneous Interviewing of Husbands and Wives: Making Sense of Their Stories Qualitative Inquiry. 1995;1(4).
  42. 42. Matthews LT, Beyeza-Kashesya J, Cooke I, Davies N, Heffron R, Kaida A, et al. Consensus statement: Supporting Safer Conception and Pregnancy For Men And Women Living with and Affected by HIV. AIDS and behavior. 2017. pmid:28501964.
  43. 43. Conroy AA, McGrath N, van Rooyen H, Hosegood V, Johnson MO, Fritz K, et al. Power and the association with relationship quality in South African couples: Implications for HIV/AIDS interventions. Soc Sci Med. 2016;153:1–11. pmid:26859436; PubMed Central PMCID: PMCPMC4788545.
  44. 44. Crankshaw TL, Voce A, Butler LM, Darbes L. Expanding the relationship context for couple-based HIV prevention: Elucidating women's perspectives on non-traditional sexual partnerships. Soc Sci Med. 2016;166:169–76. pmid:27566046; PubMed Central PMCID: PMCPMC5023493.
  45. 45. Jiwatram-Negron T, El-Bassel N. Systematic review of couple-based HIV intervention and prevention studies: advantages, gaps, and future directions. AIDS and behavior. 2014;18(10):1864–87. pmid:24980246; PubMed Central PMCID: PMCPMC4507500.
  46. 46. Lewis MA, McBride CM, Pollak KI, Puleo E, Butterfield RM, Emmons KM. Understanding health behavior change among couples: an interdependence and communal coping approach. Soc Sci Med. 2006;62(6):1369–80. pmid:16146666.
  47. 47. Bhatia D, Harrison A, Kubeka M, Milford C, Wilson IB, Psaros C, et al. The Role of Relationship Dynamics and Gender Inequalities as Barriers to HIV-serostatus Disclosure: Qualitative Study among Women and Men Living with HIV in Durban, South Africa. Frontiers in Public Health. 2017, in press.
  48. 48. Heffron R, Mugo N, Ngure K, Bukusi E, Odoyo J, Katabira E, et al., editors. PrEP and ART reduce HIV transmission between members of HIV serodiscordant couples during pregnancy and pregnancy attempts. Abstract THPDC0102. AIDS 2016; 2016; Durban.
  49. 49. Mmeje O, Eshiwani S, Ngoroge B, Davies N. HIV and Infertility: A pragmatic approach for evaluation and management in low-resource settings. Abstracts FRWA0301-06. AIDS2016; Durban, South Africa. 2016.
  50. 50. Mmeje O, Njoroge B, Akama E, Leddy A, Breitnauer B, Darbes L, et al. Perspectives of healthcare providers and HIV-affected individuals and couples during the development of a Safer Conception Counseling Toolkit in Kenya: stigma, fears, and recommendations for the delivery of services. AIDS Care. 2016;28(6):750–7. pmid:26960581.
  51. 51. Ngure K, Heffron R, Curran K, Vusha S, Ngutu M, Mugo N, et al. I Knew I Would Be Safer. Experiences of Kenyan HIV Serodiscordant Couples Soon After Pre-Exposure Prophylaxis (PrEP) Initiation. AIDS Patient Care STDS. 2016;30(2):78–83. pmid:26836236; PubMed Central PMCID: PMCPMC4753630.
  52. 52. Phiri S, Feldacker C, Chaweza T, Mlundira L, Tweya H, Speight C, et al. Integrating reproductive health services into HIV care: strategies for successful implementation in a low-resource HIV clinic in Lilongwe, Malawi. J Fam Plann Reprod Health Care. 2016;42(1):17–23. pmid:25902815; PubMed Central PMCID: PMCPMC4717379.
  53. 53. Rao A, Baral S, Phaswana-Mafuya N, Lambert AA, Kose Z, McIngana M, et al. Future pregnancy intentions and knowledge of methods for safer conception among female sex workers in Port Elizabeth, South Africa. Abstract #TUAD0302. AIDS2016; Durban, South Africa. 2016.
  54. 54. Matthews L, Mukherjee J. Strategies for harm reduction among HIV-affected couples who want to conceive. AIDS and behavior. 2009;13(Supplement 1):S5–S11.
  55. 55. Medley A, Garcia-Moreno C, McGill S, Maman S. Rates, barriers and outcomes of HIV serostatus disclosure among women in developing countries: implications for prevention of mother-to-child transmission programmes. Bull World Health Organ. 2004;82(4):299–307. pmid:15259260; PubMed Central PMCID: PMCPMC2585956.
  56. 56. King R, Katuntu D, Lifshay J, Packel L, Batamwita R, Nakayiwa S, et al. Processes and outcomes of HIV serostatus disclosure to sexual partners among people living with HIV in Uganda. AIDS and behavior. 2008;12(2):232–43. pmid:17828450.
  57. 57. Colombini M, James C, Ndwiga C, Integra t, Mayhew SH. The risks of partner violence following HIV status disclosure, and health service responses: narratives of women attending reproductive health services in Kenya. Journal of the International AIDS Society. 2016;19(1):20766. pmid:27037140; PubMed Central PMCID: PMCPMC4819069.
  58. 58. Conroy AA, Wong LH. How reliable are self-reports of HIV status disclosure? Evidence from couples in Malawi. Soc Sci Med. 2015;144:28–37. pmid:26379084; PubMed Central PMCID: PMCPMC4609636.
  59. 59. Campbell JI, Eyal N, Musiimenta A, Haberer JE. Ethical Questions in Medical Electronic Adherence Monitoring. J Gen Intern Med. 2016;31(3):338–42. pmid:26358284; PubMed Central PMCID: PMCPMC4762813.
  60. 60. Pintye J, Ngure K, Curran K, Vusha S, Mugo N, Celum C, et al. Fertility Decision-Making Among Kenyan HIV-Serodiscordant Couples Who Recently Conceived: Implications for Safer Conception Planning. AIDS Patient Care STDS. 2015;29(9):510–6. pmid:26301703; PubMed Central PMCID: PMCPMC4553369.
  61. 61. Nieves CI, Kaida A, Seage GR 3rd, Kabakyenga J, Muyindike W, Boum Y, et al. The influence of partnership on contraceptive use among HIV-infected women accessing antiretroviral therapy in rural Uganda. Contraception. 2015;92(2):152–9. pmid:25983013.
  62. 62. Kaida A, Kabakyenga J, Bwana M, Bajunirwe F, Bennett K, Haberer JE, et al. High planned partner pregnancy incidence among HIV-positive men in rural Uganda: Implications for comprehensive safer conception services for men, accepted as oral abstract. IAS/AIDS 2016; Durban, South Africa2016.
  63. 63. World_Health_Organization. Engaging men and boys in changing gender-based inequity in health: Evidence from programme interventions. Geneva: WHO, 2007.
  64. 64. World_Health_Organization. Integrating gender into HIV/AIDS programmes in the health sector. Geneva: WHO, 2009.
  65. 65. World Health Organization: Department of Gender WaH. Policy approaches to engaging men and boys in achieving gender equality and health equity. Geneva: WHO, 2010.
  66. 66. Barker G, Ricardo C, Nascimento M, Olukoya A, Santos C. Questioning gender norms with men to improve health outcomes: evidence of impact. Global public health. 2010;5(5):539–53. Epub 2009/06/11. pmid:19513910.
  67. 67. Greig A, Peacock D, Jewkes R, Msimang S. Gender and AIDS: time to act. Aids. 2008;22 Suppl 2:S35–43. Epub 2008/07/25. pmid:18641466; PubMed Central PMCID: PMC3356155.
  68. 68. Khidir H, Psaros C, Greener L, O'Neil K, Mathenjwa M, Mosery FN, et al. Developing a Safer Conception Intervention for Men Living with HIV in South Africa. AIDS and behavior. 2017. pmid:28194587.
  69. 69. Higgins JA, Mathur S, Eckel E, Kelley L, Nakyanjo N, Sekamwa R, et al. Importance of relationship context in HIV transmission: results from a qualitative case-control study in Rakai, Uganda. Am J Public Health. 2014;104(4):612–20. pmid:24524490; PubMed Central PMCID: PMCPMC4025686.
  70. 70. Ware NC, Wyatt MA, Haberer JE, Baeten JM, Kintu A, Psaros C, et al. What's love got to do with it? Explaining adherence to oral antiretroviral pre-exposure prophylaxis for HIV-serodiscordant couples. Journal of acquired immune deficiency syndromes. 2012;59(5):463–8. pmid:22267018
  71. 71. Crankshaw TL, Matthews LT, Giddy J, Kaida A, Ware NC, Smit JA, et al. A conceptual framework for periconception HIV transmission risk reduction among HIV serodiscordant couples. Reproductive Health Matters. 2012;20(39):50–60. PubMed Central PMCID: PMCPMC3608509.
  72. 72. Wall KM, Kilembe W, Vwalika B, Haddad LB, Lakhi S, Onwubiko U, et al. Sustained effect of couples' HIV counselling and testing on risk reduction among Zambian HIV serodiscordant couples. Sex Transm Infect. 2017;93(4):259–66. pmid:28082662.
  73. 73. Allen S, Meinzen-Derr J, Kautzman M, Zulu I, Trask S, Fideli U, et al. Sexual behavior of HIV discordant couples after HIV counseling and testing. Aids. 2003;17(5):733–40. pmid:12646797.
  74. 74. Grabbe K, Stephenson R, Vwalika B, Ahmed Y, Vwalika C, Chomba E, et al. Knowledge, use, and concerns about contraceptive methods among sero-discordant couples in Rwanda and Zambia. J Womens Health (Larchmt). 2009;18(9):1449–56. pmid:19708802; PubMed Central PMCID: PMCPMC2825682.
  75. 75. Stephenson R, Vwalika B, Greenberg L, Ahmed Y, Vwalika C, Chomba E, et al. A randomized controlled trial to promote long-term contraceptive use among HIV-serodiscordant and concordant positive couples in Zambia. J Womens Health (Larchmt). 2011;20(4):567–74. pmid:21410332; PubMed Central PMCID: PMCPMC3115412.
  76. 76. Ugandan Ministry of Health. Consolidated Guidelines for prevention and treatment of HIV in Uganda (December 2016) Kampala, Uganda: Ugandan Ministry of Health 2016 [23 June 2017]. Available from: https://elearning.idi.co.ug/pluginfile.php/83/mod_page/content/50/CONSOLIDATEDGUIDELINESFORPREVENTIONANDTREATMENTOFHIVINUGANDA.PDF.
  77. 77. Muhindo R, Nakalega A, Nankumbi J. Predictors of couple HIV counseling and testing among adult residents of Bukomero sub-county, Kiboga district, rural Uganda. BMC public health. 2015;15:1171. pmid:26603280; PubMed Central PMCID: PMCPMC4659154.
  78. 78. Darbes LA, van Rooyen H, Hosegood V, Ngubane T, Johnson MO, Fritz K, et al. Uthando Lwethu ('our love'): a protocol for a couples-based intervention to increase testing for HIV: a randomized controlled trial in rural KwaZulu-Natal, South Africa. Trials. 2014;15:64. pmid:24552199; PubMed Central PMCID: PMCPMC3936910.
  79. 79. Matthews LT, Moore L, Crankshaw TL, Milford C, Mosery FN, Greener R, et al. South Africans with recent pregnancy rarely know partner's HIV serostatus: implications for serodiscordant couples interventions. BMC public health. 2014;14:843. Epub 2014/08/16. pmid:25124267; PubMed Central PMCID: PMC4246447.
  80. 80. Matthews LT, Milford C, Kaida A, Ehrlich MJ, Ng C, Greener R, et al. Lost Opportunities to Reduce Periconception HIV Transmission: Safer Conception Counseling By South African Providers Addresses Perinatal but not Sexual HIV Transmission. Journal of acquired immune deficiency syndromes. 2014;67 Suppl 4:S210–7. Epub 2014/12/02. pmid:25436820; PubMed Central PMCID: PMC4251914.
  81. 81. Schwartz SR, Bassett J, Sanne I, Phofa R, Yende N, Van Rie A. Implementation of a safer conception service for HIV-affected couples in South Africa. Aids. 2014;28 Suppl 3:S277–85. Epub 2014/07/06. pmid:24991901.
  82. 82. Crankshaw TL, Mindry D, Munthree C, Letsoalo T, Maharaj P. Challenges with couples, serodiscordance and HIV disclosure: healthcare provider perspectives on delivering safer conception services for HIV-affected couples, South Africa. Journal of the International AIDS Society. 2014;17:18832. Epub 2014/03/19. pmid:24629843; PubMed Central PMCID: PMC3956311.
  83. 83. Bott S, Obermeyer CM. The social and gender context of HIV disclosure in sub-Saharan Africa: a review of policies and practices. SAHARA J. 2013;10 Suppl 1:S5–16. pmid:23808487; PubMed Central PMCID: PMCPMC3714313.
  84. 84. Obermeyer CM, Baijal P, Pegurri E. Facilitating HIV disclosure across diverse settings: a review. Am J Public Health. 2011;101(6):1011–23. pmid:21493947; PubMed Central PMCID: PMCPMC3093267.
  85. 85. Kennedy CE, Haberlen S, Amin A, Baggaley R, Narasimhan M. Safer disclosure of HIV serostatus for women living with HIV who experience or fear violence: a systematic review. Journal of the International AIDS Society. 2015;18(Suppl 5):20292. pmid:26643462; PubMed Central PMCID: PMCPMC4672457.