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Open Access

Peer-reviewed

Research Article

Identification of CkSNAP33, a gene encoding synaptosomal-associated protein from Cynanchum komarovii, that enhances Arabidopsis resistance to Verticillium dahliae

Abstract

SNARE proteins are essential to vesicle trafficking and membrane fusion in eukaryotic cells. In addition, the SNARE-mediated secretory pathway can deliver diverse defense products to infection sites during exocytosis-associated immune responses in plants. In this study, a novel gene (CkSNAP33) encoding a synaptosomal-associated protein was isolated from Cynanchum komarovii and characterized. CkSNAP33 contains Qb- and Qc-SNARE domains in the N- and C-terminal regions, respectively, and shares high sequence identity with AtSNAP33 from Arabidopsis. CkSNAP33 expression was induced by H2O2, salicylic acid (SA), Verticillium dahliae, and wounding. Arabidopsis lines overexpressing CkSNAP33 had longer primary roots and larger seedlings than the wild type (WT). Transgenic Arabidopsis lines showed significantly enhanced resistance to V. dahliae, and displayed reductions in disease index and fungal biomass, and also showed elevated expression of PR1 and PR5. The leaves of transgenic plants infected with V. dahliae showed strong callose deposition and cell death that hindered the penetration and spread of the fungus at the infection site. Taken together, these results suggest that CkSNAP33 is involved in the defense response against V. dahliae and enhanced disease resistance in Arabidopsis.

Citation: Wang P, Zhang X, Ma X, Sun Y, Liu N, Li F, et al. (2017) Identification of CkSNAP33, a gene encoding synaptosomal-associated protein from Cynanchum komarovii, that enhances Arabidopsis resistance to Verticillium dahliae. PLoS ONE 12(6): e0178101. https://doi.org/10.1371/journal.pone.0178101

Editor: Zhengguang Zhang, Nanjing Agricultural University, CHINA

Received: January 2, 2017; Accepted: May 7, 2017; Published: June 2, 2017

Copyright: © 2017 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability: The GenBank accession number of CkSNAP33 is KR011961.

Funding: This work was sponsored by the State Key Laboratory of Cotton Biology Open Fund (Grant No. CB2016B01), the “Seven Crop Breeding” National Major Project (Grant 2016YFD0101006), and the Genetically Modified Organism Breeding Major Project (Grant no. 2014ZX08005-002), the Program of National Nature Science Foundation of China (Grant no. 31071751). YH received the fundings. The funders had no role in study design.

Competing interests: The authors have declared that no competing interests exist.

Introduction

Soluble N-ethylmaleimide-sensitive factor adaptor protein receptor (SNARE) proteins were first identified in the late 1980s and have been characterized as key components for vesicle trafficking and membrane fusion in eukaryotic cells [1, 2]. These proteins contain an evolutionarily conserved domain that comprises a characteristic sequence of 60–70 amino acids [2]. Selective membrane fusion is accomplished by the interaction between SNAREs localized in the target membranes (t-SNAREs) and those anchored to the transport vesicle (v-SNAREs) [3]. These SNAREs form a SNARE complex containing a four-helix bundle comprising of the helices Qa, Qb, Qc, and R-SNARE domains, which provide the specificity and energy required for membrane fusion [3–5]. In terms of the essential role in vesicle trafficking, SNAREs are known to be involved in key functions in plants such as promoting the formation of the cell wall [1, 6, 7], interaction with ion channel-related proteins [8–10], response to abiotic stress [11–13], and participation in plant defense against pathogens [11–16].

The synaptosomal-associated protein 25 (SNAP25) class was first described in the mammalian neuron [17]. SNAP25 homologs contain two helices that are located at the N- and C-terminal regions of the SNARE complex in the mammalian neuronal synaptic membrane [17]. AtSNAP33 was the first characterized t-SNARE SNAP25 protein in plant and is involved in diverse membrane fusion processes, including cell plate formation in cytokinesis [1]. SNAP25-type proteins are essential for the growth and development of organisms; for example, loss of functional HsSNAP29, a human SNAP25-type protein, impairs endocytic recycling and cell motility, resulting in CEDNIK syndrome (cerebral dysgenesis, neuropathy, ichthyosis and palmoplantar keratoderma) [18]. Plants with loss of functional SNAP25-type proteins gradually develop large necrotic lesions on leaves and eventually result in a lethal dwarf phenotype [1, 19]. In addition, several studies have demonstrated the involvement of SNAP25-type proteins in plant defense responses. For example, OsSNAP32, a SNAP25-type protein in rice, is involved the responses to abiotic and biotic stresses [20] and overexpression of OsSNAP32 increases rice resistance to blast [15]. Expression of AtSNAP33 increases response to pathogenic infection and mechanical stimulation [21]. HvSNAP34, a SNAP25-type protein in barley, can interact with ROR2 to mediate disease resistance against powdery mildew in the plant cell wall [22]. Moreover, the ternary PEN1-SNAP33-VAMP721/722 SNARE complex is a default secretory pathway for plant immune responses [23, 24].

Cynanchum komarovii Al Iljinski is a desert plant that has been used as an herbal medicine to cure fever and cholecystitis in humans. We have previously reported that protein extracts of C. komarovii seeds possess strong antifungal activity [25–27]. Transformation of Arabidopsis with CkTLP and CkChn134 resulted in plant that showed resistance to the pathogenic fungus Verticillium dahliae [26]. This antifungal property stimulated the present study in which we sought to determine whether a gene encoding a SNAP25-type protein from C. komarovii could be used to improve crop resistance to V. dahliae via a transgenic engineering strategy.

In this study, we cloned and characterized CkSNAP33, a gene encoding a t-SNARE SNAP25-type protein, from C. komarovii and determined its expression at the mRNA level. We found that CkSNAP33 transgenic Arabidopsis lines were larger in size than wild type (WT) plants and that overexpression of CkSNAP33 enhanced Arabidopsis resistance to V. dahliae. The possible defense response was investigated by histological analyses of cell death and callose deposition.

Material and methods

Plant and fungal growth conditions and treatments

C. komarovii was grown in a mixture of soil and vermiculite (2:1, w/w), in a greenhouse at 16–28°C under a 16/8-h photoperiod. Arabidopsis seeds (Columbia ecotype) were sterilized with 75% ethanol and 4% NaClO solution and then sowed onto Murashige-Skoog (MS) plates (1×MS salts, 1×MS vitamins, 2% sucrose, 0.8% agar, pH 5.7). After vernalization for 3 days at 4°C, the plates were incubated in chamber under 16-h light (22°C) /8-h dark(20°C) conditions. After 10 days, the seedlings were then transplanted into pots containing a mixture of soil and vermiculite (1:1, w/w) under the same condition.

The highly aggressive defoliating isolate Vd991 of V. dahliae was cultured on potato dextrose agar (PDA) at 25°C for 7days, and then inoculated into Czapek liquid medium (0.3% NaNO3, 0.1% K2HPO4, 0.05% MgSO4·7H2O, 0.05% KCl, 0.001% FeSO4, 3% sucrose, pH 7.2). After culture for 7 days, the suspension was harvested by filtration through four layers of cheesecloth and then the culture density was adjusted to 107 conidia/mL before further use.

Four-week-old seedlings of C. komarovii were gently removed from the soil and cleaned with water. The seedlings were placed into the solutions containing 1 mM salicylic acid (SA) and 20 mM H2O2, respectively. For pathogen-infection treatment, the roots of the seedlings were inoculated with V. dahliae conidial suspension for 10 min. For mechanical wounding, a hemostat was pressed across the stem. Control samples were treated with sterile water. The seedlings subjected to different treatments were harvested at appropriate times,—frozen in liquid nitrogen and used for RNA extraction. Three replicate experiments were carried out.

Gene cloning and sequence analyses

Total RNA from C. komarovii seedilings was isolated using an extraction kit (Promega, WI, USA). Polyadenylated mRNA was obtained using a PolyATract mRNA Isolation System (Promega). A cDNA library was constructed as previously described [28] and propagated on 140-mm plates approximately 106 clones were obtained. The conserved region of AtSNAP33 isolated from Arabidopsis [1], was labeled with 32P-dUTP and used as probe for positive plaques by in situ hybridization. Four positive plaques were obtained after three screening rounds. The corresponding clones were sub-cloned into pBlueScript II SK (+) using the in vivo excision protocol provided by the manufacturer (Stratagene, USA).

The nucleotide sequence of CkSNAP33 and the deduced amino acid sequence were investigated via an NCBI/Blast search. The theoretical isoelectric point (pI) and the molecular mass were calculated by ProtParam; the transmembrane domain of CkSNAP33 was predicted using the TMHMM online tool. A phylogenetic analysis was carried out in MEGA 5.1. Clustal Omega and SMART were used for multiple sequence alignment and domain prediction, respectively.

CkSNAP33 expression analysis

Total RNAs from C. komarovii seedlings in different treatments were isolated using RNA extraction Kit (Promega). RNA quality was evaluated using agarose gel electrophoresis and a NanoDrop 2000 spectrophotometer (ThermoFisher Scientific, Waltham, MA, USA). In total, 2 μg total RNA was reverse-transcribed to first-strand cDNA by High Capacity RNA-to-cDNA kit (Applied Biosystems, CA, USA). Real-time PCR was conducted to detect the CkSNAP33 transcript level, using CkEF-1-α (HQ849463) from C. komarovii as the reference gene with the specific primers qEF1α-F/qEF1α-R [25]. A pair of primers (q33-F/q33-R) was designed to amplify a 178-bp fragment of CkSNAP33 spanning the first intron (the sequences of the primers mentioned here are listed in S1 Table). For real-time PCR, a SYBR Premix Ex Taq™ II kit (TaKaRa, Dalian, China) and an ABI 7500 thermocycler (Applied Biosystems) were used for quantifying gene expression. PCR was performed in a 20-μL volume under the following conditions: 95°C denaturation for 30 s, followed by 40 cycles of 95°C for 5 s and 60°C for 34 s. The relative level of CkSNAP33 expression was determined using the comparative 2-ΔΔCt method. Three independent replicates of each PCR assay were performed.

Generation of transgenic Arabidopsis plants

The full-length CkSNAP33 cDNA fragment was amplified using primers ZW33-F/ZW33-R with the XbaI/SalI restriction sites at the 5′ and 3′ ends, respectively. The resulting PCR fragment was inserted into a modified pCAMBIA 1300 vector with a hygromycin phosphotransferase (hpt II) gene and a green fluorescent protein (GFP) gene under the control of a super promoter [29]. The recombinant construct vector (S5A Fig) was introduced into Arabidopsis Columbia ecotype via Agrobacterium tumefaciens (strain GV3101) transformation. Transgenic Arabidopsis seeds were screened on MS plates containing 25 μg/mL hygromycin B and then verified by PCR analysis of genomic DNA using the vector-specific primers 1300-F/1300-R. T3 generation seedlings were used for further experiments.

Subcellular localization

The Super 1300-CkSNAP33-GFP plasmid was precipitated onto gold beads and transformed into onion epidermal cells by microprojectile bombardment using PDS-1000/He™ (Bio-Rad, CA, USA). Subcellular localization of fluorescent protein was observed at 488 nm by confocal laser scanning biological microscope FLUOVIEW FV1000 (OLYMPUS, Tokyo, Japan). Plasmolysis was induced by incubating samples in 0.8 M mannitol for 10 min.

Morphological examination of transgenic plants

Arabidopsis seedlings were grown in a vertical orientation on MS plates for 15 days and root lengths were measured using vernier calipers (Qualitot 141–494, Guilin, China). Four-week-old Arabidopsis plants were grown in pots and were photographed with a digital camera (Nikon D90, Tokyo, Japan). The fresh weight of the plant aerial portions was measured using an analytical balance (Ohaus AR2130, NJ, USA), and the leaf area of rosette leaves was computed using coordinate paper (Baolishi, Zhejiang, China) and Photoshop CS5 (Adobe Systems, San Jose, CA, USA).

V. dahliae inoculation and disease investigation

For V. dahliae inoculation of Arabidopsis, three-week-old seedlings were removed from the soil, washed with water, and dried briefly with blotting paper to remove excess water. The roots were then immersed in a beaker containing a fresh spore suspension (107 conidia/mL) for 10 min. The seedlings were transplanted into fresh soil immediately and kept in a high-humidity environment for 12 h at 25°C. Seedlings in the control group were mock-treated with sterile water. The disease index (DI) was measured periodically for 21days post-inoculation (dpi).

The quantification of V. dahliae biomass was performed as previously described [30]. The plants at 21 dpi were removed from the soil and ground to powder in liquid nitrogen; genomic DNA was extracted by the cetyltrimethyl ammonium bromide (CTAB) method and was quantified by NanoDrop 2000 spectrophotometer (ThermoFisher Scientific) and agarose gel electrophoresis. The biomass at the DNA level was determined by real-time PCR as described above. The primers used for the quantification of V. dahliae were qVd-F/qVd-R. AtEF1-α (NM_100666.3) expression level was used as the internal standard to normalize differences in DNA template amounts (the primer sequences are given in S1 Table).

Real-time PCR was performed as described above to determine the transcription level of the genes pathogenesis-related protein 1 (PR1) and pathogenesis-related protein 5 (PR5) in Arabidopsis infected with V. dahliae at 6 dpi. The specific primers for PR1 and PR5 were qPR1-F/qPR1-R and qPR5-F/qPR5-R, respectively. AtEF1-α was used as the endogenous control and was detected using the primer pair AtEF1α-F/AtEF1α-R (the sequences of the primers mentioned here are listed in S1 Table).

Histological detection of cell death and callose deposition in leaves

To investigate the resistance mechanism induced by expression of CkSNAP33 in transgenic Arabidopsis, leaves were inoculated with 5-μL of V. dahliae suspension (107 conidia/mL) and incubated at 25°C in a moist chamber. The infected leaves were then stained with aniline blue for callose deposition at 24 hours post-inoculation (hpi) [31] and subjected to trypan blue staining to show fungal structure and cell death at 48 hpi [32, 33]. Callose deposition was monitored by fluorescence microscopy (Nikon C1) and cell death was observed and imaged under an optical microscope (Nikon ECLIPSE Ti, Tokyo, Japan).

Statistical analysis

All experiments and measurements were performed with three replicates per treatment. Statistical analyses were performed with SPSS.16 using one-way ANOVA. Data are presented as mean ± SE. Significant differences were determined at the 5% level of significance and asterisks are used to indicate p-values: *p < 0.05, **p < 0.01.

Results

Characterization of CkSNAP33

The target cDNA was obtained from C. komarovii using colony in situ hybridization, and designated CkSNAP33 (GenBank accession number: KR011961). The full-length cDNA of CkSNAP33 is 1298 bp long, including a 906 bp ORF that encodes a putative protein of 301 amino acids (S1 Fig) with a predicted pI of 6.63 and a calculated molecular weight of approximately 33.28 kD. It has a genomic DNA sequence of 1666 bp, with five exons and four introns (S2 Fig); this is similar to exon—intron structure of OsSNAP32 [20]. A TMHMM analysis indicated that CkSNAP33 did not have a transmembrane domain (S3 Fig). Multi-sequence alignment analysis of CkSNAP33 with other SNAP25-type proteins revealed that it shares high identity with AtSNAP33 (68.75%), AtSNAP30 (58.54%), OsSNAP32 (55.48%), and HvSNAP34 (53.38%). CkSNAP33 also contains a Qb-SNARE domain from Ala-102 to Gly-169 and a Qc-SNARE domain from Ala-231 to Leu-298 (Fig 1); these domains are the characteristic dual heptad repeat SNARE motifs of SNAP25 proteins [34]. Some SNAP25-type proteins contain palmitoylation modifications at a conserved cysteine cluster to mediate membrane anchorage; these four cysteine residues do not exist in CkSNAP33, other plant SNAP25-type proteins, or HsSNAP29 (Fig 1). The phylogenetic tree of the SNAP25 homologies from various organisms showed three differentiated main branches (plants, animals, and yeasts), and CkSNAP33 was clustered into a clade that included AtSNAP33 (NP_200929.1) and AtSNAP29 (NP_196405.1) (Fig 2). Tissue-specific expression of CkSNAP33 in C. komarovii showed higher in roots than that in stems and leaves (S4 Fig).

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Fig 1. Protein sequence alignment of CkSNAP33 with other SNAP25 proteins.

HsSNAP25a (AAH10647.1) and HsSNAP29 (O95721.1) from Homo sapiens, OsSNAP32 (AAW82752.1) from Oryza sativa L., HvSNAP34 (AAP79417.1) from Hordeum vulgare, and AtSNAP33 (Q9S7P9.1) from Arabidopsis thaliana. Conserved residues are shaded in black and similar residues in gray. Positions in Qb-and Qc-SNARE domains that contribute to stabilizing ionic or hydrophobic interaction with other SNARE proteins are marked using asterisks and dots, respectively. The four cysteine residues involved in palmitoylation and membrane association of SNAP25 are indicated using arrow. Multiple amino acid sequence analyses were performed using Clustal Omega and the multiple alignment file was shaded using the BoxShade program.

https://doi.org/10.1371/journal.pone.0178101.g001

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Fig 2. Phylogenetic tree of SNAP25 proteins.

The phylogenetic tree was constructed using the neighbor-joining method with MEGA 5.1, and bootstrap values from 1,000 replicates are indicated at the nodes.

https://doi.org/10.1371/journal.pone.0178101.g002

CkSNAP33 expression is induced by H2O2, SA, V. dahliae, and wounding

CkSNAP33 transcript levels in C. komarovii seedlings were measured under various stresses. During H2O2 treatment, CkSNAP33 was gradually elevated and reached its peak at 3 h, and decreased thereafter (Fig 3A). CkSNAP33 transcription was immediately up-regulated after SA treatment and maintained at a high level until 48 h (Fig 3B). In seedlings inoculated with V. dahliae, CkSNAP33 transcription was significantly up-regulated at 1 and 3 hpi and gradually declined at 6 and 12 hpi (Fig 3C). CkSNAP33 transcript level reached a maximum at 60 min after wounding treatment (Fig 3D).

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Fig 3. The expression patterns of CkSNAP33 under different conditions.

(A) 20 mM H2O2. (B) 1 mM salicylic acid (SA). (C) Verticillium dahliae. (D) Wounding. Data were collected from three independent biological repeats. Results are expressed as mean ± standard error (SE; n = 3). Asterisks show significance difference (* p < 0.05; **p < 0.01).

https://doi.org/10.1371/journal.pone.0178101.g003

CkSNAP33 is located at plasma membrane

The subcellular localization of CkSNAP33 was examined using CkSNAP33-GFP fusion protein expressed in onion epidermal cell (Fig 4). The GFP fluorescence was observed at the cell wall or at the plasma membrane (Fig 4A). The samples were treated with 0.8 M mannitol to differentiate between the plasma membrane and the cell wall localization; the GFP fluorescence after plasmolysis revealed that CkSNAP33 was localized at plasma membrane (Fig 4B).

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Fig 4. Subcellular localization of CkSNAP33-GFP fusion protein.

(A) Confocal images of transformed onion epidermal cells. (B) Confocal images of transformed onion epidermal cells after plasmolysis.

https://doi.org/10.1371/journal.pone.0178101.g004

Overexpression of CkSNAP33 promotes the growth of Arabidopsis plants

A total of 11 independent Arabidopsis lines overexpressing CkSNAP33 were obtained by selection for hygromycin B resistance and genomic DNA-PCR analysis (S5B Fig). Real-time PCR suggested that a range of expression levels of CkSNAP33 was present in the transgenic lines (S5C Fig). L12 showed the highest CkSNAP33 expression, while L3 and L10 showed an intermediate level of expression. L12 and L3 were selected for further functional analysis.

The L3 and L12 lines had longer roots than WT seedlings at 15 days (Fig 5A and 5C). In four-week-old seedlings grown in soil, transgenic plants were generally larger than WT (Fig 5B). The fresh weight of transgenic plants was about 1.9-fold greater than that of WT (Fig 5D) and leaf area in transgenic plants was about 2.36–3.10-fold greater than that of WT (Fig 5E).

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Fig 5. Developmental phenotypes of transgenic and wild type (WT) plants.

(A) 15-day-old Arabidopsis seedlings of WT and representative CkSNAP33 overexpression lines (L3, L12) grown on vertically orientated Murashige-Skoog plates. Scale bar represents 5 mm. (B) Morphology of the rosettes of 4-week-old Arabidopsis plants. (C) Root length of 15-day-old seedlings. (D) Fres weight of 4week-old seedlings. (E) Leaf area of 4-week-old seedlings. Data are mean ± SE (n = 3). Asterisks show significant differences (**p < 0.01).

https://doi.org/10.1371/journal.pone.0178101.g005

Overexpression of CkSNAP33 confers Arabidopsis enhanced disease resistance to V. dahliae

CkSNAP33 transgenic lines and WT Arabidopsis were inoculated with V. dahliae. At 21 dpi, WT plants showed serious wilting; whereas plants of the transgenic lines showed less intense symptoms (Fig 6A). Disease indexes (DI) were recorded from 6 dpi to 21 dpi (Fig 6B). At 21 dpi, WT plants showed a higher DI (76.39) compared with plants of the transgenic L3 and L12 lines (58.33 and 54.17, respectively) (Fig 6B). The relative fungal biomass assay gave consistent results to the DI analysis and V. dahliae biomass in the transgenic lines was lower than that in WT (Fig 6C), implying that CkSNAP33 overexpression enhanced Verticillium wilt resistance in Arabidopsis.

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Fig 6. Transgenic expression of CkSNAP33 enhances resistance to Verticillium dahliae.

(A) Disease phenotypes in WT and two transgenic plants (L3, L12) infected by V. dahliae. Images were captured at 21 dpi. (B) Disease index of WT and transgenic Arabidopsis plants at the indicated days after inoculation. Data are means ± SE (n = 3). (C) Fungal biomass in infected Arabidopsis plants at 21 dpi. Results are mean ± SE (n = 3). Asterisks represent significance differences (*p < 0.05; **p < 0.01).

https://doi.org/10.1371/journal.pone.0178101.g006

Trypan blue staining of leaf tissues at 6 dpi was used to identify fungal hyphae in leaves of WT and transgenic plants (Fig 7A and 7B). Leaves from WT showed more free hyphae (Fig 7A and 7C). Leaves from plants of the L3 and L12 lines showing CkSNAP33 overexpression showed trailing cell death around the hyphae (Fig 7B and 7D). Cell death in the CkSNAP33 transgenic lines appeared to be associated with the enhanced resistance. Real-time PCR results for expression of PR genes indicated that PR1 and PR5 transcript levels increased in infected Arabidopsis plants and these levels in L3 and L12 were considerably higher than those in WT at 6 dpi, especially for PR5 (Fig 7E and 7F).

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Fig 7. Inducing the hypersensitive response (HR) and pathogenesis-related genes in CkSNAP33 over-expressing Arabidopsis.

Trypan blue staining of leaf sections from WT (A) and transgenic plants (B) at 6 dpi with V. dahliae. Scale bar represents 500 μm. (C) Normal growth of V.dahliae hyphae (hy) across the leaf surface of WT. Close-up of the disease lesion regions from (A). Scale bar represents 200 μm. (D) V.dahliae induced programmed cell death (arrows) around the hyphae (hy). Close-up of the disease lesion regions from (B). Scale bar represents 200 μm. Real-time PCR analysis for the transcript levels of PR5 (E) and PR1 (F) in WT and transgenic lines before (0 dpi) and 6 days after inoculation with V. dahliae (6 dpi). Data are mean ± SE (n = 3), asterisks represent significant differences (*p < 0.05; **p < 0.01).

https://doi.org/10.1371/journal.pone.0178101.g007

To further investigate the function of CkSNAP33 in resistance to V. dahliae, 5 μL conidial suspension was dropped onto Arabidopsis leaves. At 6 dpi, WT leaves showed extensive necrosis; by comparision, slight etiolation of the leaves was observed at the inoculation site in transgenic lines (Fig 8A and 8B). The transgenic plants showed clear resistance to V. dahliae at the inoculation site, especially plants in L12.

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Fig 8. Over-expression of CkSNAP33 in Arabidopsis hinders the expansion of V.dahliae in leaves.

Disease symptoms (A) and lesion size (B) in the WT and the transgenic lines at 6 dpi, Data are means ± SE (n = 3) and asterisks represent significant differences (**p < 0.01) in (B). Trypan blue staining of V. dahliae-infected leaves of WT (C) and transgenic lines (D); scale bars represent 3 mm. (E) and (F) Close-ups of the disease areas from (C) and (D). The growth of free hyphae (arrows) exceeds the cell death zone in (E). Scale bars represent 50 μm.

https://doi.org/10.1371/journal.pone.0178101.g008

Trypan blue staining at 48 hours post inoculation (hpi) revealed that WT plants not only showed large necrotic patches but the hyphae also extended into the adjacent areas (Fig 8C and 8E). In contrast, the transgenic lines showed lower necrosis and no hyphae were detected (Fig 8D and 8F), indicating that hypersensitive reaction (HR) induced cell death is exhibited in transgenic lines, which is capable of restricting the development of pathogens [35].

SNARE proteins are involved in the resistance to penetration of pathogens at the cell wall [22]. The results of the aniline blue assay at 24 hpi indicate that the transgenic lines accumulated more callose than WT leaves at infection sites (Fig 9). Overexpression of CkSNAP33 might increase the transport of callose synthases to the infection sites and enhance callose accumulation, then inhibit invasion by V. dahliae.

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Fig 9. Callose deposition in Verticillium dahliae-infected leaves of WT and CkSNAP33 over-expressing plants.

(A) Control WT leaf. (B) V. dahliae WT infected leaf. (C) Control transgenic leaf. (D) V. dahliae infected transgenic leaf. Scale bar represents 50 μm.

https://doi.org/10.1371/journal.pone.0178101.g009

Discussion

In eukaryotic cells, SNARE proteins can form a complex that allows membrane fusion between vesicles, organelles, and plasma membrane. All SNAREs contain a sequence termed the SNARE motif that is predisposed to form coiled-coils [2]. The neuronal SNARE core complex contains motifs of syntaxin 1A (Qa-SNARE), SNAP25 (Qb- and Qc- SNARE) and synaptobrevin/VAMPII(R-SNARE) and forms a parallel four-helix bundles[2, 36]. The present study cloned and characterized a SNAP25 homolog from C. komarovii, termed CkSNAP33.Similarly to other SNAP25 proteins, CkSNAP33 contains an N-terminal Qb-SNARE motif with Gln-141 and a Qc-SNARE motif with Gln-270 in the C-terminal region, which contribute to the formation of the “zero ionic layer” with Qa-SNARE and R-SNARE. We showed that CkSNAP33 is located at the plasma membrane; this intracellular distribution is consistent with the localizations of several SNAP25-type proteins in plants [1, 20]. However, unlike syntaxin and synaptobrevin, SNAP25-type proteins lack transmembrane domain at C-terminal [37]. However, some SNAP25 homologs are associated with membranes and these associations appear to depend on post-translational palmitoylation of cysteine residues located at the center of the peptide sequence [38, 39]. However, the cysteine cluster is not present in CkSNAP33, AtSNAP33, HsSNAP29 nor HsSNAP34. Whether the cysteine at position 120 of SNAP33 is modified by lipid at its only or hydrophobic post-translational modifications at another residue remains to be determined.

Expression of SNAP25-type genes in plants can be induced by a variety of defense-related chemicals and pathogens. For instance, exogenous SA and thiadiazole-7-carbonic acid S-methyl ester (BTH) induce expression of AtSNAP33 [21]. OsSNAP32 expression is significantly activated by H2O2 in rice [20]. In C. komarovii, we showed here that transcript levels of CkSNAP33 also increased during treatment with H2O2 and SA. This finding indicated that CkSNAP33 expression was inducible by a signal molecule such as SA and H2O2 and that CkSNAP33 was involved in the defense-signaling network in the plant. Furthermore, we found that the transcription of CkSNAP33 was induced by V. dahliae. OsSNAP32 can also be activated by rice blast (Magnaporthe grisea) [40] and the expression of AtSNAP33 is induced by several pathogens, including Plectosporium tabacinum, Peronospora parasitica, and Pseudomonas syringae pv tomato [21]. AtSNAP33 expression is regulated by SA-dependent and SA-independent pathways after pathogen infection, [21], our results suggested that CkSNAP33 was involved in an Arabidopsis defense response against V. dahliae through either the SA or H2O2 signaling pathway.

SNAP33 is essential for the growth and development of plants [1, 19]. AtSNAP33 shows high expression in various tissues, including roots, stems, leaves, and flowers [1, 4]. In rice, OsSNAP32 is highly expressed in leaves and expression in roots is low [20]. The high level of expression of CkSNAP33 in roots of C. komarovii may be associated with the particular root traits and small leaves of desert plants, and this observation suggested that CkSNAP33 is preferentially expressed in vigorous tissues in plant. We found that the transgenic Arabidopsis lines that overexpressed CkSNAP33 had longer roots and were physically larger than WT plants. AtSNAP33 interacts with the cytokinesis-specific syntaxin KNOLLE (KN) at cell plate formation in plant cytokinesis [1]. This indicates that CkSNAP33 overexpression might increase membrane fusion associated with cytokinesis in transgenic Arabidopsis. However, the mechanisms through which overexpression of CkSNAP33 can promote the growth of Arabidopsis plant remain to be determined.

Penetration resistance and programmed cell death are two major strategies in plants to restrict the invasion of biotrophic fungal pathogens [41].Here, we found a reduced disease phenotype and disease index, low pathogen biomass, and increased levels of PR1 and PR5 transcripts in transgenic Arabidopsis carrying CkSNAP33 indicating enhanced resistance to V. dahliae. Papillae, callose-containing cell-wall appositions, are effective barriers during the relatively early stages of pathogen invasion [42, 43] and the papillae in grapevine can block penetration and haustoria formation against powdery mildew[41]. Barley HvSNAP34 protein and its binding partner ROR2 mediate penetration resistance against Blumeria graminis.f.sp. hordei at the cell wall [22]. Furthermore, PEN1 and SNAP33 are incorporated into powdery mildew at the papillary matrix [31]. In our study, increased callose deposition was induced in the infected leaves of transgenic Arabidopsis compared to WT leaves, indicating that CkSNAP33 participated in papilla formation in Arabidopsis and was involved the early defense responses against V. dahliae. Plant pathogens are known to activate the H2O2 and SA pathways and trigger defense responses in the form of the hypersensitive response (HR), leading to hypersensitive cell death and systemic acquired resistance in the host plant [24, 44, 45]. The trypan blue assay showed HR-induced cell death occurred in transgenic lines at the infection site, and that this limited pathogen in the death cell zone. These results suggested that CkSNAP33 was involved in the early defense responses at the cell wall and that a defense signal activated the HR to enhance disease resistance in Arabidopsis.

In summary, CkSNAP33 is a ubiquitous synaptosomal-associated protein, belonging to SNAP25-type t-SNARE family. CkSNAP33 overexpression promoted plant growth in transgenic Arabidopsis and enhanced disease resistance to V. dahliae through callose deposition and HR-induced cell death. This study contributes to the elucidation of the function of SNAP25 homolog proteins in pathogen defense. However, further research is necessary to define the role of CkSNAP33 in transport of defense signal molecules or products via membrane fusion.

Supporting information

S1 Fig. Nucleotide sequence and deduced amino acid sequence of CkSNAP33.

The Qb-SNARE and Qc-SNARE domains are shown in gray. The amino acids highlighted in yellow are conserved glutamine residues of the Q-SNARE domains.

https://doi.org/10.1371/journal.pone.0178101.s001

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S2 Fig. Schematic structure of CkSNAP33.

https://doi.org/10.1371/journal.pone.0178101.s002

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S3 Fig. CkSNAP33 lacks a transmembrane domain according to TMHMM analysis.

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S4 Fig. Tissues specific expression pattern of CkSNAP33 in C. komarovii by real-time PCR.

Data were collected from three independent biological repeats. Results are shown as means ±SE (n = 3). Asterisks indicate significant differences (* p<0.05, **p<0.01).

https://doi.org/10.1371/journal.pone.0178101.s004

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S5 Fig. Genetic transformation of Arabidopsis with CkSNAP33 and identification of transgenic lines.

(A) Schematic representation of the modified pCAMBIA 1300 vector with CkSNAP33 under the control of a super promoter. (B) Genomic DNA-PCR analysis of genomic DNA from hygromycin B resistant lines. (C) Real-time PCR confirmed the expression of CkSNAP33 in transgenic lines.

https://doi.org/10.1371/journal.pone.0178101.s005

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S1 Table. List of primers used in this study.

https://doi.org/10.1371/journal.pone.0178101.s006

(DOCX)

Author Contributions

  1. Conceptualization: PW YH FL.
  2. Data curation: PW YH.
  3. Formal analysis: PW YS.
  4. Funding acquisition: FL YH.
  5. Investigation: PW XZ XM.
  6. Project administration: PW YH.
  7. Resources: PW YS NL.
  8. Software: PW XM.
  9. Supervision: YH FL.
  10. Validation: PW XZ XM.
  11. Visualization: PW FL YH.
  12. Writing – original draft: PW YH.
  13. Writing – review & editing: PW YH FL.

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