A relevant proportion of human immunodeficiency virus (HIV) infected patients is co-infected with the hepatitis C virus (HCV). HCV co-infection in HIV-positive patients is associated with faster progression of liver disease in comparison to HCV mono-infection. Natural killer (NK) cells critically modulate the natural course of HCV infection. Both HIV and HCV mono-infection are associated with alterations of the NK cell pool. However, little data is available concerning phenotype and function of NK cells in HIV/HCV co-infection.
A total of 34 HIV/HCV co-infected, 35 HIV and 39 HCV mono-infected patients and 43 healthy control persons were enrolled into this study. All HIV-positive patients were under effective antiretroviral therapy. NK cell phenotype, IFN-γ production and degranulation were studied by flow cytometry.
NK cell frequency in HIV/HCV co-infection was significantly lower than in healthy individuals but did not differ from HIV and HCV mono-infection. HIV/HCV co-infection was associated with significantly decreased expression of the maturation/differentiation markers CD27/62L/127 on NK cells but increased expression of CD57 compared to healthy controls. Of note, expression also differed significantly from HCV mono-infection but was similar to HIV mono-infection, suggesting a pronounced impact of HIV on these alterations. Similar findings were made with regard to the NK cell receptors NKG2A/C and NKp30. More importantly, NK cells in co-infection displayed a highly impaired functional activity with significantly lower IFN-γ production and degranulation than in healthy donors as well as HIV and HCV mono-infection, suggesting a synergistic effect of both viruses.
Citation: Kaczmarek DJ, Kokordelis P, Krämer B, Glässner A, Wolter F, Goeser F, et al. (2017) Alterations of the NK cell pool in HIV/HCV co-infection. PLoS ONE 12(4): e0174465. https://doi.org/10.1371/journal.pone.0174465
Editor: Johan K. Sandberg, Karolinska Institutet Department of Medicine Solna, SWEDEN
Received: August 17, 2016; Accepted: March 9, 2017; Published: April 5, 2017
Copyright: © 2017 Kaczmarek et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All relevant data are within the paper and its Supporting Information files.
Funding: This work was supported by a BONFOR grant (O-107.0116 [DK]), the German Research Foundation (DFG SFB/TR 57 [US, JN], DFG KR4521/1-1 [BK], the H. W. and J. Hector Foundation (grant number M69 [JN]) and the German Center for Infection Research (DZIF TTU 04.810 [JN]).
Competing interests: The authors have declared that no competing interests exist.
Due to similar transmission routes of infection a relevant proportion of human immunodeficiency virus (HIV)-positive patients is co-infected with the hepatitis C virus (HCV). HIV/HCV co-infection is associated with a faster progression to liver fibrosis and cirrhosis, resulting in higher mortality compared to HCV mono-infected individuals[2–8]. Accordingly, liver-associated mortality has become a major cause of death in HIV-positive (HIV(+)) persons under combined anti-retroviral therapy (cART).
Incomplete restoration of the immune system in HIV patients despite effectively blocked HIV replication is considered to importantly contribute to this phenomenon[9–12]. In this context, persistent dysregulation of the natural killer (NK) cell pool is of especial interest, as NK cells have been shown to effectively block HCV replication[13,14] and to display an anti-fibrotic activity.
Both HIV and HCV mono-infection are associated with significant perturbations of NK cells. For instance, a reduction of absolute NK cell numbers was found in HIV(+) as well as in HCV(+) patients[16,17] and both HIV and HCV mono-infection as well as HIV/HCV co-infection have been observed to be associated with the appearance of a highly dysfunctional subset of CD56- CD16+ NK cells, characterized by a poor cytotoxic activity[18–23]. In addition, both viral infections are characterized by altered expression patterns of activating NK cell receptors (NKR)[11,24,25] although reports on NKR expression in chronic hepatitis C are controversial[24–31]. Besides these shared alterations in NK cell phenotype and functions observed in HIV and HCV mono-infection, there are also infection-specific differences regarding perturbations of the NK cell pool. As an example, expression of the inhibitory C-type lectin receptor NKG2A has repeatedly been demonstrated to be increased in chronic hepatitis C[24,32], whereas HIV infection is associated with a decreased frequency of NKG2A[10,33]. Furthermore, Meier et al were able to detect significant differences in the production of IFN-γ between HIV and HCV. Moreover, anti-viral[11,34,35] as well as anti-fibrotic NK cell functions have been shown to be impaired in HIV patients even in the context of effective cART.
Although a large number of studies analyzed the impact of HIV and HCV mono-infection, respectively, on phenotype and functions of NK cells, little is known regarding alterations of this lymphocyte subset in HIV patients chronically co-infected with HCV.
Here, we show that HIV/HCV co-infection is associated with a significant dysregulation of the circulating NK cell pool, and present data suggesting that dysregulation mainly reflects alterations observed in HIV mono-infection.
Materials and methods
Our cross-sectional study was conducted among patients who attended the outpatient clinic of the Department of Internal Medicine I at the university hospital in Bonn, Germany, between the beginning of 2013 and the end of 2015. Gender, age, CD4+ T-cell counts, HIV-1 and HCV RNA viral load as well as liver enzyme levels were retrieved from patients’ files. A total of 108 patients, including 35 HIV mono-infected, 39 HCV mono-infected and 34 HIV/HCV co-infected individuals, all from the Cologne/Bonn area in Germany, were enrolled into this study. All HIV(+) patients were under effective cART, containing a combination of two nucleoside/nucleotide reverse transcriptase inhibitors (NRTI) and either a ritonavir-boosted protease inhibitor (PI) or a non-NRTI (NNRTI), with HIV RNA loads persistently below the level of detection. As a control group 43 healthy HIV(-)/HCV(-) individuals were studied. A detailed description of patient characteristics is given in Table 1 (Table 1). Informed written consent was obtained from all patients. The study had been approved by the local ethics committee of the University of Bonn (Bonn, Germany).
Isolation of PBMC
Peripheral blood mononuclear cells (PBMC) were isolated from venous blood by Ficoll-Paque (PAA, Germany) density gradient centrifugation as described elsewhere and cryopreserved. PBMC were cultured in RPMI medium (PAA, Germany) supplemented with 10% fetal calf serum (FCS) (Biochrom, Germany) and 1% penicillin/streptomycin (PAA, Germany) at 37°C and 5% CO2.
HuH7 HCVreplicon cells
HuH7 HCVreplicon cells were kindly provided by V. Lohmann and R. Bartenschlager (University of Heidelberg, Heidelberg, Germany). Cells were grown in high-glucose Dulbecco’s modified Eagle’s medium (DMEM) (4.5 g/L) supplemented with glutamine (PAA Laboratories GmbH, Cölbe, Germany), 10% fetal calf serum (FCS), 1% nonessential amino acids (Biochrom AG, Berlin, Germany) and 1% penicillin/streptomycin (PAA, Germany). Blasticidin S hydrochloride (3 μg/mL) and G418 (1 mg/mL; PAA, Germany) were added to cells containing subgenomic replicons. HuH7 HCVreplicon cells were passaged twice a week and were seeded at a dilution of 1:3.
I) NK cell analysis: Frequency and phenotype
Cryopreserved PBMC were thawed, washed with phosphate buffered saline (PBS) and stained with anti-CD3 and anti-CD56 fluorochrome-labeled antibodies for detection of NK cells by fluorescence activated cell sorting (FACS). In addition, cells were stained with different combinations of the following fluorochrome-labeled antibodies: anti-CD27, anti-CD57, anti-CD62L, anti-CD69, anti-CD127, anti-NKG2A, anti-NKG2C, anti-NKG2D, anti-NKp30, anti-NKp46, anti-CD107a and anti-INF-γ (Table 2). Isotype controls were used to set up gates and to determine positive and negative populations, respectively (S1 Fig). Dead cells were excluded by use of Zombie Aqua Fixable Viability Kit (Biolegend, Fell, Germany). After incubation of the cells with 1 to 10 μl of antibody solution (depending on antibody) and washing with PBS, samples were analyzed on a FACSCanto II flow cytometer using CellQuest Pro (BD Biosciences) and FlowJo 7.5 software packages (TreeStar Inc., Ashland, OR, USA). To ensure consistent cytometer settings Rainbow Calibration Particles (Biolegend, Fell, Germany) were used. Fig 1 depicts the complete gating strategy for identification of NK cells as well as CD56dim and CD56bright subsets (Fig 1).
Representative gating strategy for the identification of CD56dim and CD56bright NK cell subsets. 1st upper panel: The population of peripheral blood mononuclear cells (PBMC) was localized by forward (FSC) and side scatter (FSC) gating using areal scaling (FSC-A, SSC-A). 2nd upper panel: Dead cells were excluded by use of Zombie Aqua Fixable Viability Kit (Biolegend, Fell, Germany). 3rd upper panel: Cell aggregates were eliminated by width parameters for FSC and SSC (FSC-W, SSC-W). Lower panels: NK cells were defined as CD3- CD56+ cell population. CD56dim and CD56bright NK cells were discriminated by low versus high expression of CD56.
II) NK cell analysis: CD107a degranulation assay
NK cell degranulation was assessed in response to HuH7 HCVreplicon cells. To this end, cryopreserved PBMC were thawed and cultured at 37°C in complete medium without any exogenously added cytokines. After 12 hours, PBMC were washed, resuspended and pre-stimulated with recombinant human interleukin (rhIL)-2 (25 U/mL; R&D Systems, USA). Then, cells were co-incubated with HuH7 HCVreplicon cells at an effector/target (E:T) ratio of 1:1 in the presence of anti-CD107a to assess degranulation, as described before.
III) NK cell analysis: IFN-γ secretion
Cryopreserved PBMC were thawed, pre-stimulated with rhIL-2 (25 U/mL; R&D Systems, USA) followed by co-incubation with HuH7 HCVreplicon cells at an E:T ratio of 1:1 at 37°C for 5 hours. Brefeldin A (10 μg/mL; Sigma-Aldrich, St. Louis, MO, USA) was added after 1 hour of co-culture. Next, cells were harvested and washed, followed by intracellular staining with anti-IFN-γ and flow cytometry analysis.
Frequencies of cell sub-populations between the different cohorts were compared using Kruskal-Wallis tests. Test multiplicity was controlled by a false discovery rate (FDR) procedure accounting for dependency among statistical tests . FDR-adjusted P values <0.05 were considered statistically significant. Statistical analyses were performed using GraphPad Prism Version 5.0a (GraphPad Software Inc, San Diego, CA, USA) and the SPSS 17.0 statistical package (SPSS, Chicago, IL, USA).
Frequency of NK cells in HIV/HCV co-infection
First, we analyzed the frequency of NK cells among our study cohorts (Fig 2A). The frequency of circulating NK cells, assessed as percentage of CD3- CD56+ cells among PBMC, was significantly lower in HIV/HCV co-infection compared to healthy controls but did not differ significantly from that observed in HIV and HCV mono-infected patients, respectively (Fig 2B, left panel). HIV/HCV co-infection was associated with an increased frequency of CD56bright NK cells, whereas its proportion of CD56dim and CD56bright cells was similar to both mono-infected groups, respectively (Fig 2B, right panel).
A) Representative dot plots of flow cytometric analyses. B) 1st panel: Frequency of circulating NK cells, displayed as percentage of vital, singlet peripheral blood mononuclear cells (PBMC) in healthy donors (n = 43), HIV mono-infected patients (HIV; n = 35), HCV mono-infected individuals (HCV; n = 39) and co-infected patients (HIV/HCV; n = 34). 2nd panel: Distribution of CD56dim and CD56bright NK cells, displayed as percentage of total NK cells in healthy donors (n = 43), HIV (n = 35), HCV (n = 39) and HIV/HCV (n = 34). * p≤0.05, ** p≤0.01, *** p≤0.001, “n.s.” non-significant.
Expression of maturation/differentiation markers in HIV/HCV co-infection
Next, we studied the NK cell maturation/differentiation status in our cohorts (Fig 3A). Compared to healthy controls we found HIV/HCV co-infection to be associated with a decreased frequency of CD56bright NK cells expressing markers characteristic for less mature NK cells, such as CD27, CD62L and CD127 (Fig 3B), whereas the proportion of CD57+ NK cells was significantly increased (Fig 3B, lower right panel). Similar observations were made in HIV mono-infected patients. In contrast, NK cells from HCV mono-infected individuals displayed an expression of CD27, CD62L, CD127 and CD57 molecules similar to that observed in healthy controls.
A) Representative dot plots of flow cytometric analyses. B) Expression of maturation/differentiation markers was determined by flow cytometry on total (CD56pos), CD56dim and CD56bright natural killer (NK) cells. 1st upper panel: Expression of CD27 in healthy donors (n = 18), HIV mono-infected patients (HIV; n = 20), HCV mono-infected individuals (HCV; n = 17) and HIV/HCV co-infected patients (HIV/HCV; n = 17). 2nd upper panel: Expression of CD62L in healthy donors (n = 15), HIV (n = 11), HCV (n = 13) and HIV/HCV (n = 12). 1st lower panel: Expression of CD127 in healthy donors (n = 16), HIV (n = 17), HCV (n = 16) and HIV/HCV (n = 14). 2nd lower panel: expression of CD57 in healthy donors (n = 16), HIV (n = 11), HCV (n = 17) and HIV/HCV (n = 15). * p≤0.05, ** p≤0.01, *** p≤0.001, “n.s.” non-significant.
Expression of NK cell receptors in HIV/HCV co-infection
Then, we analyzed the expression of NK cell receptors (NKR) which have been shown to critically regulate NK cell functions (Figs 4A and 5A). As is shown in Fig 4B we found HIV mono- as well as HIV/HCV co-infection to be associated with a significantly lower frequency of NKG2A-expressing NK cells than healthy controls whereas patients with chronic hepatitis C displayed a significantly higher frequency of NKG2A+ NK cells compared to all other studied groups (Fig 4B, upper left panel). Moreover, we found the frequency of CD56dim NKG2C+ NK cells to be significantly higher in HIV/HCV co- and HIV mono-infected patients than in healthy individuals (Fig 4B, upper right panel). In addition, we observed the frequency of NKG2D+ NK cells to be reduced in co-infected patients (Fig 4B, lower left panel), whereas NKG2D surface expression density (RFI) was not affected (Fig 4B, lower right panel). Similar observations were made in patients mono-infected with HCV. However, CD56bright NK cells in HCV patients displayed an increased surface expression density of NKG2D. With respect to expression of the natural cytotoxicity receptors (NCR) NKp30 and NKp46 we found HIV(+) patients to display a significantly lower frequency of NKp30+ NK cells than HCV mono-infected patients and healthy controls, irrespective of HCV co-infection (Fig 5B, left panel), whereas the frequency of NKp46+ NK cells was reduced in all patient cohorts (Fig 5B, right panel).
A) Representative dot plots of flow cytometric analyses. B) Expression of NKR was determined by flow cytometry on total (CD56pos), CD56dim and CD56bright NK cells. 1st upper panel: Expression of the C-type lectin receptor NKG2A in healthy donors (n = 15), HIV mono-infected patients (HIV; n = 12), HCV mono-infected individuals (HCV; n = 25) and HIV/HCV co-infected patients (HIV/HCV; n = 20). 2nd upper panel: Expression of NKG2C in healthy controls (n = 15), HIV (n = 15), HCV (n = 21) and HIV/HCV (n = 12). 1st lower panel: Expression of NKG2D in healthy persons (n = 16), HIV (n = 12), HCV (n = 20) and HIV/HCV (n = 14). 2nd lower panel: Surface expression density (RFI) of NKG2D in healthy persons (n = 16), HIV (n = 11), HCV (n = 10) and HIV/HCV (n = 14). * p≤0.05, ** p≤0.01, *** p≤0.001, “n.s.” non-significant.
A) Representative dot plots of flow cytometric analyses. B) Expression of NCR was determined by flow cytometry on total (CD56pos), CD56dim and CD56bright NK cells. 1st panel: Expression of NKp30 in healthy donors (n = 18), HIV mono-infected patients (HIV; n = 17), HCV mono-infected individuals (HCV; n = 32) and HIV/HCV co-infected patients (HIV/HCV; n = 16). 2nd panel: Expression of NKp46 in healthy controls (n = 28), HIV (n = 25), HCV (n = 29) and HIV/HCV (n = 17). * p≤0.05, ** p≤0.01, *** p≤0.001, “n.s.” non-significant.
HIV and HCV infection affect NK cell functionality
Finally, we studied the NK cell activation status (Fig 6A) and functional capacity (Fig 7A). As is depicted in Fig 6B, the frequency of NK cells expressing the activation marker CD69 was not different in HIV/HCV co-infected patients from healthy controls, while HCV mono-infected patients showed the highest frequency in comparison to all other groups (Fig 6B). With respect to NK cell function, we observed degranulation of rhIL-2-stimulated NK cells following co-incubation with HuH7 HCVreplicon cells to be significantly impaired in all virus-infected patient groups as compared to healthy controls (Fig 7B, upper right panel). Of note, this defect was most pronounced in HIV/HCV co-infected patients, which were found to have a significantly lower frequency of CD107a+ NK cells than patients mono-infected with HIV and HCV, respectively. No such differences were seen when NK cell degranulation was studied in the absence of target cells (Fig 7B, upper left panel). Analyzing IFN-γ production of rhIL-2 stimulated NK cells we observed a significantly reduced percentage of IFN-γ+ NK cells in HIV mono-infected patients compared to healthy and HCV mono-infected individuals (Fig 7B, lower right panel). However, this functional defect was strongest in HIV infected patients co-infected with HCV. IFN-γ production of unstimulated NK cells was similar between the four study groups (Fig 7B, lower left panel).
A) Representative dot plots of flow cytometric analyses. B) Expression of the activation marker CD69 was determined by flow cytometry on total (CD56pos), CD56dim and CD56bright NK cells in healthy donors (n = 12), HIV mono-infected patients (HIV; n = 11), HCV mono-infected individuals (HCV; n = 10) and HIV/HCV co-infected patients (HIV/HCV; n = 15). * p≤0.05, ** p≤0.01, *** p≤0.001, “n.s.” non-significant.
A) Representative dot plots of flow cytometric analyses. B) Expression of markers was determined by flow cytometry on total (CD56pos), CD56dim and CD56bright NK cells. 1st upper panel: Surface expression of CD107a in healthy donors (n = 10), HIV mono-infected patients (HIV; n = 8), HCV mono-infected individuals (HCV; n = 10) and HIV/HCV co-infected patients (HIV/HCV; n = 12). 2nd upper panel: Surface expression of CD107a following co-incubation of peripheral blood mononuclear cells (PBMC) with HuH7 HCVreplicon cells in healthy controls (n = 27), HIV (n = 27), HCV (n = 21) and HIV/HCV (n = 12). 1st lower panel: Unstimulated PBMC were co-cultured with HuH7 HCVreplicon cells. Production of IFN-γ was determined by intracellular staining of NK cells from healthy controls (n = 12), HIV (n = 8), HCV (n = 10) and HIV/HCV (n = 19). 2nd lower panel: rhIL-2 stimulated PBMC were co-cultured with HuH7 HCVreplicon cells. Production of IFN-γ was determined in healthy controls (n = 20), HIV (n = 19), HCV (n = 11) and HIV/HCV (n = 12). * p≤0.05, ** p≤0.01, *** p≤0.001, “n.s.” non-significant.
NK cells have been shown to importantly contribute to anti-HCV immune responses and to modulate HCV-associated liver fibrosis[10,12,15,29]. However, little is known regarding the effects of HIV/HCV co-infection on functions and phenotype of NK cells. Here, we show that HIV/HCV co-infection is associated with a significant dysregulation of the NK cell pool, including both phenotypic as well as functional alterations.
NK cell development is considered to proceed sequentially from CD56bright to CD56dim NK cells[39–41]. This process is associated with a gradual decrease in CD27, CD62L and CD127, all highly expressed on circulating CD56bright cells, together with a progressive increase in receptors characteristic of mature cells, such as CD57[18,42–50]. Of note, we found HIV/HCV co-infection to be associated with a shift towards a more mature NK cell phenotype as frequency of NK cells expressing CD27, CD62L and CD127 was significantly lower than in healthy controls, whereas the proportion of mature CD57 expressing NK cells was increased. Moreover, we found the frequency of NK cells positive for NKG2A, which has been shown to be highly expressed on less mature NK cells but to display a stepwise decrease during terminal NK cell differentiation[42,43,48], to be significantly lower in co-infected patients than in healthy individuals. Similar observations were made in HIV mono-infected patients. This is in line with findings by Hong et al. who demonstrated a decline of less differentiated NK cells in chronic HIV mono-infection. The exact mechanisms underlying HIV-driven rapid NK cell maturation are only incompletely understood but may at least in part be the result of HIV-induced ongoing activation of T- and B-cells[51–57]. Of note, markers of immune activation have been shown to be associated with plasma levels of microbial products in HIV infection. Thus, increased levels of circulating microbial products, resulting from microbial translocation, have been proposed to represent a major cause of HIV-induced chronic T- and B-cell activation. Thus, similar mechanisms may also play a role with respect to NK cells. NK cell maturation has been also observed in other viral diseases such as infection with the cytomegalovirus (CMV) and, thus, is not specific to HIV(+) individuals[47,58]. In HCV mono-infected patients, however, such a mechanism seems to be of minor if any relevance because the NK cell maturation status did not differ significantly from that found in healthy controls.
Several reports demonstrated significant alterations of the NK cell receptor expression in HIV and HCV mon-infection, respectively[11,21,24–26,28,30,59–62]. Of note, some of these perturbations have been found to differ significantly between both viral infections. The most prominent example relates to the expression of the inhibitory NKG2A receptor, which has been shown to be up-regulated in chronic HCV mono-infection[24,25,28,30,63] but reduced in HIV mono-infection[11,62,63]. These findings could be confirmed in the present study. More importantly, we found HIV/HCV co-infection to be associated with low expression of NKG2A similar to that seen in patients with HIV mono-infection. In addition, we found the expression of the activating receptors NKp30 and NKp46 to be down-regulated in both HIV mono-infected as well as in HIV/HCV co-infected patients. This is in line with earlier reports which also demonstrated a reduced surface expression of activating NK cell receptors in HIV(+) patients[59,60]. Activation of NK cells has been proposed to result in down-regulation of NKp30 and NKp46[21,26]. Thus, low expression of NKp30 and NKp46 in HIV mono-infected and HIV/HCV co-infected patients might be due to HIV-induced NK cell activation. However, NK cell expression of the activation marker CD69 was not altered in HIV infected patients, irrespective of HCV co-infection, while elevated in HCV mono-infected patients, which rather argues against such a mechanism, since NKp30 expression in HCV mono-infected patients did not differ from that seen in healthy controls but was significantly higher than in HIV(+) individuals.
With respect to NK cell functions we observed HIV mono-infection to be associated with significantly impaired IFN-γ production, which is in line with previous reports[34,64,65]. No such dysregulation of NK cell activity could be found in HCV mono-infection, whereas HIV/HCV co-infected individuals displayed the lowest IFN-γ production. The degranulation rate of NK cells was reduced in all infected individuals while, similar to IFN-γ production, the most pronounced reduction could be found in co-infection. The exact mechanisms involved in impaired NK cell functions in HIV/HCV co-infection remain to be clarified. In HIV mono-infected patients, however, perturbed NK cell maturation as well as altered NK cell receptor expression have been suggested to cause dysfunctionality of NK cells[59,60,66]. Thus, it is conceivable that similar mechanisms might also play a role in co-infected patients.
Our study has several limitations. First, we only included HIV(+) patients under effective therapy and, thus, the effect of ongoing HIV replication on NK cells could not be assessed. Second, the number of cells that were available for our study was limited. Therefore, we could not analyze all markers in all patients. Moreover, a number of surface molecules important for NK cell functions and/ or differentiation, such as killer cell immunglobulin-like receptors (KIR), could not be tested. Third, we focused on CD56+ cells but did not examine the CD56- counterpart. Accordingly, further studies are needed to characterize the full extent of alterations in the NK cell compartment in HIV/HCV co-infection.
Taken together, our data indicate that HIV/HCV co-infection is associated with significant alterations of the NK cell pool, which mainly reflect alterations observed in HIV mono-infection.
S1 Fig. Expression of measured markers.
Peripheral blood mononuclear cells (PBMC) were stained with different fluorochrome-labeled or isotype-matched antibodies and analyzed on a flow cytometer. Blue histogram profile indicates the isotype control, and red histogram indicates the specific antibody.
The authors gratefully thank Claudia Finnemann for her perfect technical assistance.
- Conceptualization: DK US JN.
- Data curation: DK PK BK AG FW FG PL US JN.
- Formal analysis: DK US JN.
- Funding acquisition: DK BK CPS JKR US JN.
- Investigation: DK PK BK AG FW FG PL.
- Methodology: DK PK BK AG FW US JN.
- Project administration: DK US JN.
- Resources: CSZ CB CPS JKR US JN.
- Supervision: CPS JKR US JN.
- Validation: DK PK BK AG FW FG PL.
- Visualization: DK PK BK AG JN.
- Writing – original draft: DK JN.
- Writing – review & editing: DK CPS JKR US JN.
- 1. Boesecke C, Rockstroh JK. Acute hepatitis C in patients with HIV. Semin Liver Dis. 2012;32: 130–137. pmid:22760652
- 2. Andreoni M, Giacometti A, Maida I, Meraviglia P, Ripamonti D, Sarmati L. HIV-HCV co-infection: epidemiology, pathogenesis and therapeutic implications. Eur Rev Med Pharmacol Sci. 2012;16: 1473–1483. pmid:23111959
- 3. Benhamou Y, Bochet M, Di Martino V, Charlotte F, Azria F, Coutellier A, et al. Liver fibrosis progression in human immunodeficiency virus and hepatitis C virus coinfected patients. The Multivirc Group. Hepatology. 1999;30: 1054–1058. pmid:10498659
- 4. Hernandez MD, Sherman KE. HIV/hepatitis C coinfection natural history and disease progression. Curr Opin HIV AIDS. 2011;6: 478–482. pmid:22001892
- 5. Operskalski EA, Kovacs A. HIV/HCV co-infection: pathogenesis, clinical complications, treatment, and new therapeutic technologies. Curr HIV/AIDS Rep. 2011;8: 12–22. pmid:21221855
- 6. Pol S, Lamorthe B, Thi NT, Thiers V, Carnot F, Zylberberg H, et al. Retrospective analysis of the impact of HIV infection and alcohol use on chronic hepatitis C in a large cohort of drug users. J Hepatol. 1998;28: 945–950. pmid:9672168
- 7. Price JC, Thio CL. Liver Disease in the HIV-Infected Individual. Clin Gastroenterol Hepatol. 2010;8: 1002–1012. pmid:20851211
- 8. Rotman Y, Liang TJ. Coinfection with Hepatitis C Virus and Human Immunodeficiency Virus: Virological, Immunological, and Clinical Outcomes. J Virol. 2009;83: 7366–7374. pmid:19420073
- 9. Dillon SM, Lee EJ, Bramante JM, Barker E, Wilson CC. The natural killer cell interferon-gamma response to bacteria is diminished in untreated HIV-1 infection and defects persist despite viral suppression. J Acquir Immune Defic Syndr. 2014;65: 259–267. pmid:24091697
- 10. Fauci AS, Mavilio D, Kottilil S. NK cells in HIV infection: paradigm for protection or targets for ambush. Nat Rev Immunol. 2005;5: 835–843. pmid:16239902
- 11. Iannello A, Debbeche O, Samarani S, Ahmad A. Antiviral NK cell responses in HIV infection: II. viral strategies for evasion and lessons for immunotherapy and vaccination. J Leukoc Biol. 2008;84: 27–49 pmid:18388299
- 12. Ye L, Wang X, Wang S, Wang Y, Song L, Hou W, et al. CD56+ T Cells Inhibit Hepatitis C Virus Replication in Human Hepatocytes. Hepatology. 2009;49: 753–762. pmid:19085952
- 13. Kokordelis P, Krämer B, Körner C, Boesecke C, Voigt E, Ingiliz P, et al. An effective IFN- mediated inhibition of HCV replication by NK cells is associated with spontaneous clearance of acute hepatitis C in HIV(+) patients. Hepatology. 2013
- 14. Li Y, Zhang T, Ho C, Orange JS, Douglas SD, Ho W-Z. Natural killer cells inhibit hepatitis C virus expression. J Leukoc Biol. 2004;76: 1171–1179. pmid:15339939
- 15. Glässner A, Eisenhardt M, Krämer B, Körner C, Coenen M, Sauerbruch T, et al. NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner. Lab Invest. 2012;92: 967–977. pmid:22449797
- 16. Meier U-C, Owen RE, Taylor E, Worth A, Naoumov N, Willberg C, et al. Shared Alterations in NK Cell Frequency, Phenotype, and Function in Chronic Human Immunodeficiency Virus and Hepatitis C Virus Infections. J Virol. 2005;79: 12365–12374. pmid:16160163
- 17. Oliviero B, Mele D, Degasperi E, Aghemo A, Cremonesi E, Rumi MG, et al. Natural killer cell dynamic profile is associated with treatment outcome in patients with chronic HCV infection. J Hepatol. 2013;59: 38–44. pmid:23499727
- 18. Björkström NK, Ljunggren H-G, Sandberg JK. CD56 negative NK cells: origin, function, and role in chronic viral disease. Trends in Immunology. 2010;31: 401–406. pmid:20829113
- 19. Cooper MA, Fehniger TA, Caligiuri MA. The biology of human natural killer-cell subsets. Trends in Immunology. 2001;22: 633–640. pmid:11698225
- 20. Mavilio D, Lombardo G, Benjamin J, Kim D, Follman D, Marcenaro E, et al. Characterization of CD56-/CD16+ natural killer (NK) cells: a highly dysfunctional NK subset expanded in HIV-infected viremic individuals. Proc Natl Acad Sci USA. 2005;102: 2886–2891. pmid:15699323
- 21. Parsons MS, Tang C-C, Jegaskanda S, Center RJ, Brooks AG, Stratov I, et al. Anti-HIV antibody-dependent activation of NK cells impairs NKp46 expression. J Immunol. 2014;192: 308–315. pmid:24319263
- 22. Gonzalez VD, Falconer K, Michaëlsson J, Moll M, Reichard O, Alaeus A, et al. Expansion of CD56- NK cells in chronic HCV/HIV-1 co-infection: reversion by antiviral treatment with pegylated IFNalpha and ribavirin. Clin Immunol. 2008;128: 46–56. pmid:18495540
- 23. Gonzalez VD, Falconer K, Björkström NK, Blom KG, Weiland O, Ljunggren H-G, et al. Expansion of Functionally Skewed CD56-Negative NK Cells in Chronic Hepatitis C Virus Infection: Correlation with Outcome of Pegylated IFN-α and Ribavirin Treatment. The Journal of Immunology. 2009;183: 6612–6618. pmid:19846870
- 24. Nattermann J, Feldmann G, Ahlenstiel G, Langhans B, Sauerbruch T, Spengler U. Surface expression and cytolytic function of natural killer cell receptors is altered in chronic hepatitis C. Gut. 2006;55: 869–877. pmid:16322112
- 25. Rehermann B. Pathogenesis of chronic viral hepatitis: differential roles of T cells and NK cells. Nature Medicine. 2013;19: 859–868. pmid:23836236
- 26. Alter G, Jost S, Rihn S, Reyor LL, Nolan BE, Ghebremichael M, et al. Reduced frequencies of NKp30+NKp46+, CD161+, and NKG2D+ NK cells in acute HCV infection may predict viral clearance. J Hepatol. 2011;55: 278–288. pmid:21168454
- 27. Dessouki O, Kamiya Y, Nagahama H, Tanaka M, Suzu S, Sasaki Y, et al. Chronic hepatitis C viral infection reduces NK cell frequency and suppresses cytokine secretion: Reversion by anti-viral treatment. Biochem Biophys Res Commun. 2010;393: 331–337. pmid:20138830
- 28. Harrison RJ, Ettorre A, Little A-M, Khakoo SI. Association of NKG2A with treatment for chronic hepatitis C virus infection. Clin Exp Immunol. 2010;161: 306–314. pmid:20550548
- 29. Krämer B, Körner C, Kebschull M, Glässner A, Eisenhardt M, Nischalke H-D, et al. Natural killer p46High expression defines a natural killer cell subset that is potentially involved in control of hepatitis C virus replication and modulation of liver fibrosis. Hepatology. 2012;56: 1201–1213. pmid:22532190
- 30. Nattermann J. NK cells in acute hepatitis C. J Hepatol. 2011;55: 265–267. pmid:21236311
- 31. Oliviero B, Varchetta S, Paudice E, Michelone G, Zaramella M, Mavilio D, et al. Natural killer cell functional dichotomy in chronic hepatitis B and chronic hepatitis C virus infections. Gastroenterology. 2009;137: 1151–1160, 1160–7. pmid:19470388
- 32. Jost S, Altfeld M. Control of human viral infections by natural killer cells. Annu Rev Immunol. 2013;31: 163–194. pmid:23298212
- 33. Mavilio D, Benjamin J, Daucher M, Lombardo G, Kottilil S, Planta MA, et al. Natural killer cells in HIV-1 infection: dichotomous effects of viremia on inhibitory and activating receptors and their functional correlates. Proc Natl Acad Sci USA. 2003;100: 15011–15016. pmid:14645713
- 34. Goeser F, Glässner A, Kokordelis P, Wolter F, Lutz P, Kaczmarek DJ, et al. HIV mono-infection is associated with an impaired anti-HCV activity of NK cells. AIDS. 2015;
- 35. Goodier MR, Imami N, Moyle G, Gazzard B, Gotch F. Loss of the CD56hiCD16- NK cell subset and NK cell interferon-gamma production during antiretroviral therapy for HIV-1: partial recovery by human growth hormone. Clin Exp Immunol. 2003;134: 470–476. pmid:14632753
- 36. Kanof ME, Smith PD, Zola H. Isolation of whole mononuclear cells from peripheral blood and cord blood. Curr Protoc Immunol. 2001;Chapter 7: Unit 7.1.
- 37. Alter G, Malenfant JM, Altfeld M. CD107a as a functional marker for the identification of natural killer cell activity. J Immunol Methods. 2004;294: 15–22. pmid:15604012
- 38. Benjamini Y, Yekutieli D. The Control of the False Discovery Rate in Multiple Testing under Dependency. The Annals of Statistics. 2001;29: 1165–1188.
- 39. Chan A, Hong D-L, Atzberger A, Kollnberger S, Filer AD, Buckley CD, et al. CD56bright human NK cells differentiate into CD56dim cells: role of contact with peripheral fibroblasts. J Immunol. 2007;179: 89–94. pmid:17579025
- 40. Freud AG, Yokohama A, Becknell B, Lee MT, Mao HC, Ferketich AK, et al. Evidence for discrete stages of human natural killer cell differentiation in vivo. J Exp Med. 2006;203: 1033–1043. pmid:16606675
- 41. Yu J, Freud AG, Caligiuri MA. Location and cellular stages of natural killer cell development. Trends in Immunology. 2013;34: 573–582. pmid:24055329
- 42. Fu B, Wang F, Sun R, Ling B, Tian Z, Wei H. CD11b and CD27 reflect distinct population and functional specialization in human natural killer cells. Immunology. 2011;133: 350–359. pmid:21506999
- 43. Helden MJ van, Goossens S, Daussy C, Mathieu A-L, Faure F, Marçais A, et al. Terminal NK cell maturation is controlled by concerted actions of T-bet and Zeb2 and is essential for melanoma rejection. J Exp Med. 2015;212: 2015–2025. pmid:26503444
- 44. Hong HS, Eberhard JM, Keudel P, Bollmann BA, Ballmaier M, Bhatnagar N, et al. HIV Infection Is Associated with a Preferential Decline in Less-Differentiated CD56dim CD16+ NK Cells. J Virol. 2010;84: 1183–1188. pmid:19906929
- 45. Juelke K, Killig M, Luetke-Eversloh M, Parente E, Gruen J, Morandi B, et al. CD62L expression identifies a unique subset of polyfunctional CD56dim NK cells. Blood. 2010;116: 1299–1307. pmid:20505160
- 46. Montaldo E, Vitale C, Cottalasso F, Conte R, Glatzer T, Ambrosini P, et al. Human NK cells at early stages of differentiation produce CXCL8 and express CD161 molecule that functions as an activating receptor. Blood. 2012;119: 3987–3996. pmid:22403260
- 47. Nielsen CM, White MJ, Goodier MR, Riley EM. Functional Significance of CD57 Expression on Human NK Cells and Relevance to Disease. Front Immunol. 2013;4.
- 48. Béziat V, Descours B, Parizot C, Debré P, Vieillard V. NK Cell Terminal Differentiation: Correlated Stepwise Decrease of NKG2A and Acquisition of KIRs. PLoS ONE. 2010;5: e11966. pmid:20700504
- 49. Silva A, Andrews DM, Brooks AG, Smyth MJ, Hayakawa Y. Application of CD27 as a marker for distinguishing human NK cell subsets. Int Immunol. 2008;20: 625–630. pmid:18326863
- 50. Lopez-Vergès S, Milush JM, Pandey S, York VA, Arakawa-Hoyt J, Pircher H, et al. CD57 defines a functionally distinct population of mature NK cells in the human CD56dimCD16+ NK-cell subset. Blood. 2010;116: 3865–3874. pmid:20733159
- 51. Brenchley JM, Price DA, Schacker TW, Asher TE, Silvestri G, Rao S, et al. Microbial translocation is a cause of systemic immune activation in chronic HIV infection. Nat Med. 2006;12: 1365–1371. pmid:17115046
- 52. Marras F, Nicco E, Bozzano F, Di Biagio A, Dentone C, Pontali E, et al. Natural killer cells in HIV controller patients express an activated effector phenotype and do not up-regulate NKp44 on IL-2 stimulation. Proc Natl Acad Sci USA. 2013;110: 11970–11975. pmid:23818644
- 53. Ho HN, Hultin LE, Mitsuyasu RT, Matud JL, Hausner MA, Bockstoce D, et al. Circulating HIV-specific CD8+ cytotoxic T cells express CD38 and HLA-DR antigens. J Immunol. 1993;150: 3070–3079. pmid:8454874
- 54. Giorgi JV, Liu Z, Hultin LE, Cumberland WG, Hennessey K, Detels R. Elevated levels of CD38+ CD8+ T cells in HIV infection add to the prognostic value of low CD4+ T cell levels: results of 6 years of follow-up. The Los Angeles Center, Multicenter AIDS Cohort Study. J Acquir Immune Defic Syndr. 1993;6: 904–912. pmid:7686224
- 55. Giorgi JV, Ho HN, Hirji K, Chou CC, Hultin LE, O’Rourke S, et al. CD8+ lymphocyte activation at human immunodeficiency virus type 1 seroconversion: development of HLA-DR+ CD38- CD8+ cells is associated with subsequent stable CD4+ cell levels. The Multicenter AIDS Cohort Study Group. J Infect Dis. 1994;170: 775–781. pmid:7930717
- 56. Hazenberg MD, Stuart JW, Otto SA, Borleffs JC, Boucher CA, de Boer RJ, et al. T-cell division in human immunodeficiency virus (HIV)-1 infection is mainly due to immune activation: a longitudinal analysis in patients before and during highly active antiretroviral therapy (HAART). Blood. 2000;95: 249–255. pmid:10607709
- 57. Gonzalez VD, Landay AL, Sandberg JK. Innate immunity and chronic immune activation in HCV/HIV-1 co-infection. Clin Immunol. 2010;135: 12–25. pmid:20100670
- 58. Bengnér M, Béziat V, Ernerudh J, Nilsson B-O, Löfgren S, Wikby A, et al. Independent skewing of the T cell and NK cell compartments associated with cytomegalovirus infection suggests division of labor between innate and adaptive immunity. Age (Dordr). 2014;36: 571–582.
- 59. Bisio F, Bozzano F, Marras F, Di Biagio A, Moretta L, De Maria A. Successfully treated HIV-infected patients have differential expression of NK cell receptors (NKp46 and NKp30) according to AIDS status at presentation. Immunol Lett. 2013;152: 16–24. pmid:23538009
- 60. De Maria A, Fogli M, Costa P, Murdaca G, Puppo F, Mavilio D, et al. The impaired NK cell cytolytic function in viremic HIV-1 infection is associated with a reduced surface expression of natural cytotoxicity receptors (NKp46, NKp30 and NKp44). Eur J Immunol. 2003;33: 2410–2418. pmid:12938217
- 61. Golden-Mason L, Madrigal-Estebas L, McGrath E, Conroy MJ, Ryan EJ, Hegarty JE, et al. Altered natural killer cell subset distributions in resolved and persistent hepatitis C virus infection following single source exposure. Gut. 2008;57: 1121–1128. pmid:18372499
- 62. Mela CM, Burton CT, Imami N, Nelson M, Steel A, Gazzard BG, et al. Switch from inhibitory to activating NKG2 receptor expression in HIV-1 infection: lack of reversion with highly active antiretroviral therapy. AIDS. 2005;19: 1761–1769. pmid:16227783
- 63. Nattermann J, Nischalke HD, Hofmeister V, Kupfer B, Ahlenstiel G, Feldmann G, et al. HIV-1 infection leads to increased HLA-E expression resulting in impaired function of natural killer cells. Antivir Ther (Lond). 2005;10: 95–107.
- 64. Alter G, Teigen N, Davis BT, Addo MM, Suscovich TJ, Waring MT, et al. Sequential deregulation of NK cell subset distribution and function starting in acute HIV-1 infection. Blood. 2005;106: 3366–3369. pmid:16002429
- 65. Azzoni L, Papasavvas E, Chehimi J, Kostman JR, Mounzer K, Ondercin J, et al. Sustained impairment of IFN-gamma secretion in suppressed HIV-infected patients despite mature NK cell recovery: evidence for a defective reconstitution of innate immunity. J Immunol. 2002;168: 5764–5770. pmid:12023377
- 66. Romee R, Schneider SE, Leong JW, Chase JM, Keppel CR, Sullivan RP, et al. Cytokine activation induces human memory-like NK cells. Blood. 2012;120: 4751–4760. pmid:22983442