(A) Binding of His6-EF-Tu to sulfatide (SO3-3Galβ1Cer) at different pH values (pH 4.0, 5.0, 6.0, and 7.2). (B) Binding of His6-EF-Tu to various glycolipids: sulfatide, sulfated-lactosylceramide (SO3-3Galβ4Glcβ1Cer), galactosylceramide (Galβ1Cer), lactosylceramide (Galβ4Glcβ1Cer), and GM3 (NeuAcα3Galβ4Glcβ1Cer) at pH 5.0 and 7.2. Resonance units were measured at the start of dissociation. Error bars indicate standard deviations (n = 5). (C) Sensorgrams of the interaction of His6-EF-Tu with sulfatide at pH 5.0. Concentrations of sulfatide (from top to bottom) are as follows: 350, 300, 250, 200, and 150 nM. The KD value is described in the text.
Citation: Nishiyama K, Ochiai A, Tsubokawa D, Ishihara K, Yamamoto Y, Mukai T (2017) Correction: Identification and Characterization of Sulfated Carbohydrate-Binding Protein from Lactobacillus reuteri. PLoS ONE 12(3): e0174257. https://doi.org/10.1371/journal.pone.0174257
Published: March 14, 2017
Copyright: © 2017 Nishiyama et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.