Efficient transcriptomic sequencing of microbial mRNA derived from host-microbe associations is often compromised by the much lower relative abundance of microbial RNA in the mixed total RNA sample. One solution to this problem is to perform extensive sequencing until an acceptable level of transcriptome coverage is obtained. More cost-effective methods include use of prokaryotic and/or eukaryotic rRNA depletion strategies, sometimes in conjunction with depletion of polyadenylated eukaryotic mRNA. Here, we report use of Cappable-seq™ to specifically enrich, in a single step, Wolbachia endobacterial mRNA transcripts from total RNA prepared from the parasitic filarial nematode, Brugia malayi. The obligate Wolbachia endosymbiont is a proven drug target for many human filarial infections, yet the precise nature of its symbiosis with the nematode host is poorly understood. Insightful analysis of the expression levels of Wolbachia genes predicted to underpin the mutualistic association and of known drug target genes at different life cycle stages or in response to drug treatments is typically challenged by low transcriptomic coverage. Cappable-seq resulted in up to ~ 5-fold increase in the number of reads mapping to Wolbachia. On average, coverage of Wolbachia transcripts from B. malayi microfilariae was enriched ~40-fold by Cappable-seq. Additionally, this method has an additional benefit of selectively removing abundant prokaryotic ribosomal RNAs.The deeper microbial transcriptome sequencing afforded by Cappable-seq facilitates more detailed characterization of gene expression levels of pathogens and symbionts present in animal tissues.
Citation: Luck AN, Slatko BE, Foster JM (2017) Removing the needle from the haystack: Enrichment of Wolbachia endosymbiont transcripts from host nematode RNA by Cappable-seq™. PLoS ONE 12(3): e0173186. https://doi.org/10.1371/journal.pone.0173186
Editor: Kostas Bourtzis, International Atomic Energy Agency, AUSTRIA
Received: January 4, 2017; Accepted: February 16, 2017; Published: March 14, 2017
Copyright: © 2017 Luck et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: The mapped reads (Bowtie BAM output files) and FPKM values for assembled transcripts (Cufflinks text output files) are available in the NCBI Gene Expression Omnibus (Acession # GSE93139).
Funding: This work was funded internally by New England Biolabs, Ipswich, MA, of which all authors are employees. The specific roles of the authors are articulated in the ‘author contributions’ section. Employees and owners of New England Biolabs not on the author list did not contribute to the study design, data collection and analysis, decision to publish, or preparation of the manuscript. Critical feedback and proofreading of the manuscript was provided by additional members of the NEB.
Competing interests: All authors are employees of New England Biolabs, a manufacturer and vendor of molecular biology reagents including the Vaccinia Capping Enzyme, 3´ desthiobiotin-GTP and Next Generation Sequencing Library Preparation Kits. This does not alter their adherence to PLOS ONE policies on sharing data and materials.
Many filarial nematode species (including Wuchereria bancrofti, Brugia malayi, B. timori and Onchocerca volvulus) responsible for debilitating human diseases contain Wolbachia, an obligate intracellular endosymbiotic bacterium. In filarial nematodes, Wolbachia are found in the lateral cords of all adult worms as well as within the oocytes and developing embryos in the female reproductive tract [1–4]. Treatment with tetracycline antibiotics depletes Wolbachia leading to decreased fertility and eventually death of adult worms [5–7].
The precise nature of the essentiality of the Wolbachia-nematode symbiosis remains unclear, but, based on genomic sequences of filarial nematodes (and their respective Wolbachia counterparts), the obligate association is thought to derive from metabolite provisioning between the nematode and bacterium . Recently, other ‘omics’ approaches (transcriptomics/proteomics) have been utilized to provide more detailed functional characterization of the mutualism between Wolbachia and its nematode hosts. However, studying the endosymbiont transcriptome has proven challenging as Wolbachia transcripts are relatively few among many nematode transcripts; similar to looking for a needle in a haystack. Next Generation sequencing has provided an unprecedented depth of sequence information that has allowed transcriptomic studies of Wolbachia through the sequencing of mixed total RNA (nematode + Wolbachia) [9–11]. While these methods offer insight on potential Wolbachia-host interactions, the Wolbachia coverage remains low. This problem is not restricted to Wolbachia endosymbionts of arthropods and nematodes, but to any microbial symbiont or pathogen resident in a eukaryotic host. For mixed total RNA pools, where the relative proportion of bacterial transcript is low, strategies that allow depletion of eukaryotic rRNA with or without additional steps to deplete prokaryotic rRNA and/or eukaryotic mRNA have been developed [12,13]. These approaches involve multiple manipulations to deplete different classes of eukaryotic and prokaryotic RNA that would predominate in downstream transcriptomic sequencing. Here, we employed a recently described method called Cappable-seq , which instead of depleting unwanted RNA classes, specifically enriches the desired Wolbachia mRNA transcripts from B. malayi total RNA preparations in a single step. Cappable-seq enzymatically caps 5′ triphosphorylated RNA with biotinylated GTP using the vaccinia capping enzyme . Most eukaryotic mRNA contains a modified 7-methylguanylate (m7G) cap at the 5′-end and thus cannot be reacted with the vaccinia capping enzyme. Our use of Cappable-seq takes advantage of the fact that prokaryotic mRNA transcripts, such as those from Wolbachia, do not contain an m7G cap, but rather a 5′ triphosphate. This enables Wolbachia transcripts in a mixed pool of nematode/Wolbachia transcripts, to be selectively capped with a biotinylated GTP analog (3´ desthiobiotin-GTP), enriched on streptavidin beads and sequenced to a deeper depth than would be achievable by sequencing total nematode RNA.
The 3’ desthiobiotin-GTP was synthesized as previously described . Adult male B. malayi and microfilariae (MF) were obtained from the peritoneal cavity of one infected jird (TRS Labs, Athens, GA, USA).
B. malayi adult males and MF were homogenized separately with ceramic beads in CK14 tubes using a Minilys homogenizer (Precellys) and total RNA was extracted by organic extraction using Trizol (Thermo-Fisher Scientific, Inc.). Samples were treated with DNase I (Thermo-Fisher Scientific, Inc.) before further Trizol extraction and final purification. RNA integrity, purity and concentration were assessed using a Bioanalyzer 2100 (Agilent Technologies).
Vaccinia capping reaction
Total RNA from B. malayi MF (25 μg) or adult males (1.5 μg) was capped with 3´ desthiobiotin-GTP using the Vaccinia Capping System (New England Biolabs). Total RNA was incubated with 0.5 mM 3´ desthiobiotin-GTP, 0.1 mM S-adenosylmethionine (SAM) and 70 units of the Vaccinia Capping Enzyme in 1X Capping Buffer for 30 min at 37°C. RNA was then purified using the MEGAClear™ Transcription Cleanup Kit (ThermoFisher Scientific). Two additional washes (4 total washes) with the Wash Solution were performed on column to ensure complete removal of any excess 3´ desthiobiotin-GTP. To maximize recovery, RNA was eluted in 50 μL H2O.
Capture of 3´ desthiobiotin capped RNA
50 μL of eluted RNA was mixed with 50 μL of 10 mM Tris-HCl, pH 7.5, 500 mM NaCl, 1 mM EDTA (wash Buffer A). This mix was added to 100 μL of hydrophilic streptavidin magnetic beads (New England Biolabs) previously washed twice with wash Buffer B (10 mM Tris-HCl, pH 7.5, 50 mM NaCl) and twice with wash Buffer A (100 μL/wash). The RNA-bead mixture was incubated for 20 min at room temperature with occasional resuspension. Using a magnetic rack, the RNA-bead mixture was washed two times with wash Buffer A, followed by two washes with wash Buffer B (100 μL/wash). All washes (containing unlabeled RNA) were collected, cleaned up using the RNeasy Kit (Qiagen) and stored at −80°C until further use (‘Uncapped RNA’ sample). Beads (containing 3´ desthiobiotin capped RNA) were resuspended in wash Buffer B containing 1 mM biotin and incubated for 20 min at room temperature with occasional mixing. After placing the tube in the magnetic rack again, the supernatant (100 μL), containing the 3´ desthiobiotin capped transcripts was collected and cleaned up using the RNA Clean and Concentrator Kit™ (Zymo), and the RNA eluted in 10 μL H2O and stored at −80°C until further use (‘Capped RNA’ sample).
Library preparation and sequencing
Prior to library construction, the RNA integrity, purity and concentration of all samples were assessed on an RNA pico or nano chip using a Bioanalyzer (Agilent). Libraries were prepared from capped RNA, uncapped RNA (from bead wash steps above) and total RNA (12.9 ng/1.5 ng, 100 ng/100 ng and 100 ng/70 ng from B. malayi MF/adult males, respectively), using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England Biolabs) according to the manufacturer instructions. Library quality was assessed using a DNA high sensitivity chip on a Bioanalyzer 2100 prior to sequencing. The three libraries (total, capped and uncapped) for each sample (MF and adult male) were pooled and were sequenced on an Illumina NextSeq (75 bp single-end reads).
All data was analyzed using a local instance of Galaxy [15–17]. Sequence reads from each sample were first trimmed and assessed for quality using Sickle (Galaxy v 1.0.0, default settings)  and FastQC (Galaxy v 1.0.0) . RNA-Seq reads from each sample were mapped to the genome of the Wolbachia endosymbiont of B. malayi, wBm (version 2.2) or to the B. malayi genome (Wormbase version WS236) using the default settings of Bowtie2 (Galaxy v 0.2) . Reads aligned using Bowtie2 were assembled into transcripts using the default settings of Cufflinks (Galaxy v 0.0.7) .
Results and discussion
A total of over 137 million reads were sequenced from B. malayi adult male and microfilarial (MF) transcriptomic libraries. Over 6 million of these reads mapped to the Wolbachia endosymbiont (Table 1). While the percentage of reads mapping to B. malayi in the six RNA samples was relatively unchanged (Table 2) likely due to non-specific binding to the streptavidin beads, the percentage of reads mapping to Wolbachia was enriched in the samples treated with Cappable-seq (Table 1). Prior sequencing of total RNA from different lifecycle stages of a similar filarial nematode, Dirofilaria immitis, yielded ~0.7% average reads mappable to Wolbachia (0.02–2.11% depending on life-cycle stage) . Similarly, in the present study between 1.3% (adult male) and 1.6% (MF) of reads from B. malayi total RNA mapped to the wBm genome (Table 1). Comparatively, 4.9% (adult male) and 7.6% (MF) of the RNA captured by the Cappable-seq technique mapped to the wBm genome. This represents a 3.8-fold (adult male) or 4.8-fold (MF) enrichment in Wolbachia transcripts using Cappable-seq (Table 1). Additionally, the increased percentage of reads mapping to Wolbachia does not appear to be due to the higher number of sequenced reads in the capped library because downsampling of both the capped and total RNA libraries to 10 million reads had no effect on the percentage of reads mapping to Wolbachia (S1 Table).
As expected, the uncapped transcripts are not enriched for Wolbachia transcripts as compared to the total RNA control (1.7% vs 1.6% and 1.1% vs 1.3%, for MF and adult male samples, respectively, Table 1).
Reads mapping to wBm were assembled into transcripts and quantified into FPKM values (Fragments Per Kilobase of transcript per Million mapped reads) to assess whether transcriptomic coverage was enriched with Cappable-seq. Current annotation suggests the wBm genome contains 940 predicted transcripts, including ribosomal RNAs (rRNA) and tRNAs. Total RNA sequencing covered between ~58–69% of the wBm transcriptome with 544/940 (adult male) and 649/940 (MF) transcripts having FPKM>0 (Fig 1A and 1B, Table 3, S2 and S3 Tables). Captured RNA, following Cappable-seq, covered ~91–97% of the transcriptome with 852/940 (adult male) or 909/940 (MF) of transcripts detected (Fig 1A and 1B, Table 3, S2 and S3 Tables). While this may seem surprising that nearly all Wolbachia transcripts would be expressed, this is not unexpected with an intracellular bacterium such as Wolbachia that has a highly reduced, ‘minimal’ genome of only ~800 coding genes, versus thousands of coding genes in most bacteria. Moreover, high coverage across the Wolbachia transcriptome has been previously reported without any prokaryotic transcript enrichment for Wolbachia from Onchocerca ochengi  and for Wobachia from Dirofilaria immitis [10,11]. Importantly, however, in this study, high Wolbachia transcriptome coverage was achieved with less overall sequencing. Additionally, although not directly tested in this study, the increased coverage of the Wolbachia transciptome using Cappable-seq would more likely allow more comprehensive differential gene expression analysis as compared to sequencing total RNA.
A. Transcript coverage (FPKM) of Wolbachia genes reveals over 88% of Wolbachia transcripts from B. malayi adult male RNA were enriched using the Cappable-seq technique. A closer view of transcript abundance (inset) reveals most Wolbachia transcripts in total RNA are present in very low abundance, whereas the Wolbachia transcripts are more abundant in the capped RNA sample. Points along the y-axis are indicative of Wolbachia transcripts that were undetectable in total RNA that were detected in the capped RNA sample. B. Transcript coverage (FPKM) of Wolbachia genes reveals over 95% of Wolbachia transcripts from B. malayi MF RNA were enriched using the Cappable-seq technique. A closer view of transcript abundance (inset) reveals most Wolbachia transcripts in total RNA are present in very low abundance, whereas the Wolbachia transcripts are more abundant in the capped RNA sample. Points along the y-axis are indicative of Wolbachia transcripts that were undetectable in total RNA that were detected in the capped RNA sample.
Importantly, an additional 276 Wolbachia transcripts (29.4% of the total transcriptome) in the B. malayi MF sample and 334 Wolbachia transcripts (35.5% of the total transcriptome) in the B. malayi adult sample were detected as expressed using Cappable-seq that were not detected by sequencing total RNA (Fig 1A and 1B, Table S2). Not only were more transcripts detected, but also average transcript coverage (average FPKM value/transcript) was increased 2-fold (adult male) or 4-fold (MF) with Cappable-seq (Figs 2 and 3, Table 3).
(A) Transcript abundance of all 940 annotated Wolbachia genes (listed by geneID number) reveals over 95% of Wolbachia transcripts from B. malayi MF RNA were enriched using the Cappable-seq technique. Each transcript is indicated by in red (the FPKM value in the total RNA) and blue (the FPKM value in capped-RNA sample)(B) A closer view (note difference in y-axis scales between panel A and B) of transcript abundance reveals an enrichment in Wolbachia transcripts in the capped RNA sample.
(A) Transcript abundance of all 940 annotated Wolbachia genes (listed by geneID number) reveals over 95% of Wolbachia transcripts from B. malayi adult male RNA were enriched using the Cappable-seq technique. Each transcript is indicated by in red (the FPKM value in the total RNA) and blue (the FPKM value in capped-RNA sample)(B) A closer view (note difference in y-axis scales between panel A and B) of transcript abundance reveals an enrichment in Wolbachia transcripts in the capped RNA sample.
Comparison of the FPKM values between the capped and total RNA samples (FPKM Capped/FPKM Total) reveals an average enrichment of ~10 to 40-fold in adult male worms (n = 518 transcripts with FPKM values in both capped and total samples) and MF (n = 633 transcripts with FPKM values in both capped and total samples), respectively (S2 and S3 Tables). This enrichment value is likely underrepresented in the adult male sample due to the fact that fewer transcripts were detected in the adult male total RNA than the MF total RNA (because this enrichment value can only be derived if the transcript was detected in both the capped and total RNA samples). Additionally, it is unlikely this increase is due to the higher number of sequenced reads in the capped library because the FPKM value is normalized based on the number of reads sequenced (Fragments Per Kilobase of transcript per Million mapped reads).
Interestingly, some tRNAs transcripts were enriched by Cappable-seq (S2 and S3 Tables). It is unclear exactly why some Wolbachia tRNAs may be enriched by Cappable-seq while others are depleted. Furthermore, a number of pseudogenes were also enriched by Cappable-seq (S2 and S3 Tables). However, widespread expression of Wolbachia pseudogenes was previously noted in Wolbachia from Onchocerca ochengi , and importantly, transcript expression does not verify a functional gene product.
Some Wolbachia transcripts were actually depleted by using Cappable-seq, but this is not unexpected. For example, ribosomal RNA is rapidly processed in the cell and therefore would not be a substrate for the vaccinia capping enzyme. Application of Cappable-seq to E.coli RNA reduced the number of reads that mapped to rRNA from 85% to just 3% . In the adult male sample, we see complete depletion (0 FPKM) for all three Wolbachia ribosomal RNA transcripts, while in the MF sample, we observe a 79% depletion of the Wolbachia 23S rRNA gene, a 32% depletion of the Wolbachia 5S rRNA gene and a 76% depletion of the Wolbachia 16S rRNA gene (S3 Table). Additionally, there is widespread lateral gene transfer (LGT) from Wolbachia to its eukaryotic hosts, including B. malayi, and LGT transcription from these host genomes is known [22,23]. If these transcripts were the product of eukaryotic pol II, they would bear a 5´ m7G cap and also be depleted by Cappable-seq.
A number of metabolic relationships potentially responsible for the mutualism between B. malayi and Wolbachia have been proposed based on the genomes of the two organisms [8,24,25]. Filarial nematodes, such as B. malayi, lack genes required for de novo synthesis of purines, pyrimidines and other cofactors (heme and riboflavin), while these pathways are complete and likely functional within their Wolbachia endosymbiont . Moreover, given the essentiality of Wolbachia for nematode survival, targeting of these essential Wolbachia pathways may have detrimental effects on their parasitic nematode hosts. Unfortunately, transcriptomic analysis of these essential Wolbachia-specific, potentially therapeutic pathways, is often hindered by sequencing total RNA due to low transcript coverage of one or more genes in the pathway. Although gene expression alone does not confirm translation or gene functionality, transcript coverage of nearly every gene in the purine, pyrimidine, heme and riboflavin biosynthesis pathways was enriched using Cappable-seq. Importantly, transcripts for over one third (17/45) of the Wolbachia genes in these pathways were undetectable in B. malayi adult male total RNA, but were detected using Cappable-seq. Godel et al.  suggested a number of D. immitis Wolbachia genes that may be suitable drug targets. Specific targets that have been suggested include proteins involved in nucleic acid synthesis (DnaB), enzymes involved in fatty acid synthesis (FabZ) , and the previously identified Wolbachia target, FtsZ, a cell division protein . Of these targets, DnaB and FtsZ were enriched (~21-23-fold and ~12-27-fold, respectively) and FabZ went from undetectable in total RNA to detected in capped RNA. Transcripts for other proposed Wolbachia-specific drug targets including pyruvate phosphate dikinase (PPDK)  and cofactor-independent phosphoglycerate mutase (iPGM)  were also enriched (3 to 28-fold). Thus, Cappable-seq can be used to gather more meaningful information on the expression of these pathways and potential Wolbachia targets in the host-endosymbiont interaction.
We have used a modified Cappable-seq protocol to enrich Wolbachia transcripts from microfilarial and adult male B. malayi total RNA. We selected these different life-cycle stages since adult males typically represent a small sample size with a low level of Wolbachia transcription compared to the larger MF samples that have also much higher endosymbiont transcriptional activity . Using these samples, we obtained a ~4 to 5-fold enrichment of Wolbachia transcripts, corresponding to an average 10 to 40-fold enrichment in Wolbachia transcripts that were detected both before and after Cappable-seq. We also observed significant depletion of Wolbachia rRNA transcripts following Cappable-seq. The levels of enrichment of Wolbachia transcripts from MF and adult males were thus broadly similar despite a nearly 20-fold difference in the amount of total RNA used for Cappable-seq. The levels of Wolbachia transcript enrichment obtained by Cappable-seq surpass those obtained by alternative strategies that use combined rRNA and polyA depletion approaches . Our use of the technology to enrich Wolbachia endosymbiont transcripts from a large background of host nematode RNA allows deeper sequencing of the Wolbachia transcriptome with reduced sequencing effort and cost. With further optimization of parameters, we anticipate that even greater levels of enrichment might be realized. This method should prove applicable to other mixed RNA transcript samples that contain relatively few uncapped prokaryotic endosymbiont or pathogen RNAs in a pool of multitudinous capped eukaryotic RNAs and allow for more robust differential expression analysis of prokaryotic transcripts.
S1 Table. Downsampling to 10 million sequencing reads does not effect the percentage of read mapping to Wolbachia from B. malayi (wBm).
S2 Table. Wolbachia Transcript Quantitation from Capped and Total RNA from Adult Male B. malayi.
We thank Dr. Ira Schildkraut for advice and reagents and Dr. Laurence Ettwiller for helpful discussions. We thank Laurie Mazzola, Danielle Rivizzigno and Joanna Bybee for DNA sequencing. We thank Dr. Brad Langhorst for helpful discussions on data analysis. We also thank Dr. Clotilde Carlow, Dr. William Jack, Dr. Richard Roberts, Dr. Donald Comb and James Ellard for their continued support and guidance.
- Conceptualization: JMF BES.
- Investigation: ANL.
- Supervision: JMF.
- Writing – original draft: ANL JMF.
- Writing – review & editing: ANL JMF BES.
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