Knowledge on genetic diversity and structure of camel populations is fundamental for sustainable herd management and breeding program implementation in this species. Here we characterized a total of 331 camels from Northern Africa, representative of six populations and thirteen Algerian and Egyptian geographic regions, using 20 STR markers. The nineteen polymorphic loci displayed an average of 9.79 ± 5.31 alleles, ranging from 2 (CVRL8) to 24 (CVRL1D). Average He was 0.647 ± 0.173. Eleven loci deviated significantly from Hardy-Weinberg proportions (P<0.05), due to excess of homozygous genotypes in all cases except one (CMS18). Distribution of genetic diversity along a weak geographic gradient as suggested by network analysis was not supported by either unsupervised and supervised Bayesian clustering. Traditional extensive/nomadic herding practices, together with the historical use as a long-range beast of burden and its peculiar evolutionary history, with domestication likely occurring from a bottlenecked and geographically confined wild progenitor, may explain the observed genetic patterns.
Citation: Cherifi YA, Gaouar SBS, Guastamacchia R, El-Bahrawy KA, Abushady AMA, Sharaf AA, et al. (2017) Weak Genetic Structure in Northern African Dromedary Camels Reflects Their Unique Evolutionary History. PLoS ONE 12(1): e0168672. doi:10.1371/journal.pone.0168672
Editor: Samuel Rezende Paiva, Embrapa, BRAZIL
Received: May 18, 2016; Accepted: December 5, 2016; Published: January 19, 2017
Copyright: © 2017 Cherifi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All relevant data are within the paper and its Supporting Information files.
Funding: Part of this study (collection, DNA isolation and genotyping of samples from Sidi Barrani, Negeila and Marsa Matruh) has been achieved within the PROCAMED project funded by European Union within the ENPI-CBC-MED, reference number I.B/1.1/493. The content of the present document is under the responsibility of the Authors and could not be considered as the position of European Union.
Competing interests: The authors have declared that no competing interests exist.
The one-humped dromedary camel (Camelus dromedarius) belongs, together with the two-humped C. bactrianus and C. ferus species, to the Old-World camelids. It is generally accepted to be among the last major livestock species to have been domesticated. Domestication of dromedaries is acknowledged to have occurred in Asia, sometime not before the third millennium BCE. Despite several studies have addressed this issue [1–21], when and where the dromedary was actually domesticated is still a matter of research. Recently, Almathen et al. (2016), by using genotypic data from microsatellites and mitochondrial modern and ancient DNA, described the genetic basis of dromedary domestication, as well as the Southeast coast of the Arabian Peninsula as one possible place of origin. The first domesticated dromedary camels were probably mainly used as a source of meat and/or milk, and only later they significantly contributed, as pack animals, to the development of long distance commercial routes [12,22].
Introduction of dromedary into Africa is thought to have occurred through (i) an early sea-route (middle of the 1st millennium BCE; Epstein 1971) into Somalia, and (ii) a land-route (dates ranging 3rd millennium BCE to 7th century BCE) into transcontinental Egypt. Here, the Semitic word for “dromedary” is attested for the first time in a papyrus from the XXV dynasty, in a period corresponding to the Assyrian invasion of Egypt . Since then, dromedary bone remains are increasingly found in Egypt, but it is only in Roman times that dromedary dispersal into Northern Africa, westward of Egypt, occurred .
With the end of traditional trans-Saharan caravan routes, the dromedary has lost its central role as ship of the desert. Notwithstanding, it is still used in transport, draught and agriculture activities in several harsh regions in Africa and Asia, significantly contributing to economical and food security of local pastoralist communities . In the last years, the camel farming system has experienced rapid changes, with increasing set-up of periurban dairy farms and dairy plants, diversification of camel products and market penetration . This trend toward a more intensive production system is accompanied by increasing use of artificial insemination, higher culling rates, and higher selection pressure on the best performing animals. These practices may significantly influence genetic structure and variability of the dromedary camel populations in the near future. A characterization of the current levels of diversity is therefore urgent for those populations where this information is partial or lacking.
Overall, Egypt and Algeria count 486 thousand dromedaries  which represent an important economic and cultural resource for arid and semi-arid areas. Four camel populations, Sudani, Maghrabi, Falahi (or Fellahi, or Baladi) and Mowalled, are generally recognized in Egypt . On the contrary, several camel populations have been suggested to exist in Algeria based on phenotypic traits . More recently, Cherifi et al ., who performed a wide phenotypic and ethno-geographical survey of camels in South-Western Algeria, pointed to the lack of standardization in camel classification criteria, and to the presence of different camel types mixed within single herds. Based on a socio-geographical criterion, they identified the following populations in South-Western Algeria: Targui, characterized by clear coat colour, pronounced muscularity, and appreciated for racing; Rguibi, a multi-purpose well-adapted animal, characterized by various size variants and medium-length hairs; Azawad, slender-looking, light-colored, and hairless, also suitable for race; the small Steppe camel, currently in numerical decline, very appreciated for the quality of hair, especially for producing traditional items; the Ouled Sidi Cheikh camel, well adapted to the dry summer and the very cold winter of the highlands; the Sahraoui camel, obtained by crossing Ouled Sidi Cheikh with the Chaambi population. However, no genetic study has been yet carried out to support phenotypic and socio-geographical classification criteria. Indeed, a considerable amount of gene-flow and admixture is known to exist among different dromedary populations within Algeria and even at a cross-border level, mainly with Mali and Niger (southward), and Maroc (westward) . As knowledge on regional genetic diversity and structure is fundamental for sustainable herd management and implementation of breeding programs in this species, we investigated genetic diversity and relationships among Northern African dromedary camels using multi-allelic STR (Short Tandem Repeat, or microsatellite) markers, a tool that has been extensively shown to be useful for gaining insights into the genetic structure of livestock species . No ultimate clue of population originality and separateness being available for the considered Algerian and Egyptian dromedary populations, we decided to analyze genetic relationships in our dataset arranged both (i) into six populations and (ii) into thirteen geographic regions.
Materials and Methods
Blood samples from 198 unrelated animals were collected in EDTA tubes from seven Algerian areas (Naama, n = 5; El Bayadh, n = 17; Djelfa, n = 2; Bechar, n = 22; Tindouf, n = 34; Adrar, n = 31; Tamanrasset, n = 87) representing 79 herds and 17 municipalities distributed almost all over the entire country (Fig 1). Blood samples were also collected from 133 animals sampled in 6 Egyptian areas: Sidi Barrani (n = 24), Negeila (n = 23), Marsa Matruh (n = 16), Iking Maryut (n = 30), Birqash (n = 17), Al Qalaj (n = 23) (Fig 1). The blood used for all of the analyses was collected by veterinarians during routine blood sampling on commercial farm animals. Those animals were not linked to any experimental design and blood sampling was not performed specifically for this study, therefore no ethical authorization was required. All the samples and data processed in our study were obtained with the breeders and breeding organizations' consent. All the samples were collected in close collaboration with the Algerian Directorate of Agriculture Service (DSA), thus in perfect conformity with its ethical guidelines.The majority of Algerian samples (n = 185) belonged to one of the three main Algerian populations, named after the name of the tribe from which the herd’s owner originated (Azawad, Rguibi, Targui), the few remaining samples belonging to the endangered Chameau de steppe (n = 2), the Oueld Sidi Cheikh (n = 2) or population crosses (n = 9) (S1 Table). These thirteen animals were omitted when data arranged into different populations were analyzed. Out of the 133 Egyptian samples, 70 (those from Iking Maryut, Birqash, and Al Qalaj) belonged to one of the three main camel populations (Maghraby, Sudany, and Falahy, respectively), the remaining 63 (from Sidi Barrani, Negeila and Marsa Matruh) being of unknown breed origin (S1 Table). The latter were omitted when data arranged into different populations were analyzed. Genomic DNA was isolated from whole blood using the DNA Extraction Kit (Stratagene, USA) following manufacturer’s instructions.
Twenty STR loci, out of which 18 belonged to the recommended ISAG/FAO panel , were selected (S2 Table). Amplification of STR loci was carried out adopting three previously developed multiplex PCR reactions . PCR products were detected and discriminated by capillary electrophoresis (CE) using an ABI PRISM 310 Genetic Analyzer (Applied Biosystems); 0.5 μl of GeneScan 500 ROX (Life Technologies) were used as internal size standard. Raw CE data were analyzed using the GeneMapper software (Applied Biosystems). In order to avoid issues related with allele size standardization, genotypes were all produced in the same laboratory (Department of Biosciences, Biotechnologies, Biopharmaceutics—Bari, Italy), under the supervision of the same analyst (Rosangela Guastamacchia), and including three reference samples in any CE run. Genotypes are available as Supporting Information (S1 Dataset).
Classical population genetic parameters (allelic frequencies, number of alleles per locus, observed and expected heterozygosity), Hardy-Weinberg (HW) equilibrium, gametic unbalance, genetic differentiation (FST) were calculated using the Arlequin software package . Allelic richness and private allelic richness were estimated using HP-RARE  adopting n = 15 (30 “genes”) as rarefied samples size. The genetic structure of the populations was analysed using the unsupervised Bayesian clustering algorithms implemented in the software STRUCTURE 2.. STRUCTURE HARVESTER  was adopted to visualize STRUCTURE outputs and estimate Evanno’s DeltaK statistics . To test for consistency among runs, each K value (number of assumed clusters in the population) from 1 to 12 was tested 30 times. Relationships among breeds were also explored by Neighbor network analysis using the distance of Reynolds et al. (1983)and Nei (1983) calculated by Power Marker . Network representations were adopted as they allow accounting for gene flow among breeds (reticulation) and thus providing a more plausible reconstruction than linear tree representations. Neighbor networks were constructed using the Neighbor-Net algorithm  implemented in the SplitsTree4 package v. 4.13.1 . To support inference from network topologies, neighbor-joining (NJ) trees were also generated using the distance of Reynolds et al. (1983)and Nei (1983), and adopting 1000 bootstrap replications. Finally, to exclude potential population structure due to relatedness, we used COANCESTRY v.220.127.116.11 . Notably, we adopted the triadic likelihood estimator of pair-wise relatedness (TrioML), which allows for inbreeding and accounts for genotype errors in data. Proportions of pair-wise comparisons falling in different relatedness classes (R ≤ 0.25, Unrelated; 0.25 < R ≤0.5, Half-siblings; R > 0.5, Full-siblings) were calculated.
Genetic variability in the total sample and by geographical area
In this study we investigated the genetic variability of Algerian and Egyptian dromedary camels from thirteen geographical areas (Fig 1). Only few samples were collected in the central region of Algeria (Naama, El Bayadh and Djelfa) due to the presence in this area of only a limited number of herds. As a consequence, we carried out the subsequent analyses considering samples from Naama, El Bayadh and Djelfa as coming from a single region, that we labelled as “Steppe”.
All the considered loci were polymorphic (S3 Table), with the exception of CMS17 (data not shown). The locus VOLP32 was the most informative (highest FST value, 0.07). The number of alleles in the total sample ranged from 2 (CVRL8) to 24 (CVRL1D), with an average value of 9.79 ±5.31. The above loci generally displayed the lowest and the highest number of alleles also within the five Algerian and the six Egyptian regions (S4 Table). Since sample sizes varied among regions, we calculated allelic richness (S1 and S4 Tables) No remarkable difference was observed between regions in allelic richness values, that ranged from 5.1 (Marsa Matruh) to 5.9 (Adrar). Private allelic richness ranged from 0.05 (Iking Maryut) to 0.22 (Adrar) (data not shown). Average observed heterozygosity in the total sample was 0.611 ± 0.169, while gene diversity averaged over loci was 0.647 ± 0.173 (S3 Table). The highest gene diversity was observed in the Egyptian sample from Birqash (0.66 ± 0.15), the lowest being observed in the Algerian sample from Tindouf (0.62 ± 0.19) (Table 1). Eleven loci displayed a significant (P<0.05) deviation from HW proportions in the total sample (S3 Table). In all cases except one (CMS18), departure from HW proportions was due to excess of homozygous genotypes. The highest number of significantly (P<0.05) deviating loci (7) was observed for the samples from Tamanrasset (S4 Table).
Out of 171 possible pair-wise comparisons, only fifteen (8.8%) displayed significant (P<0.01) gametic unbalance in the total sample (data not shown).
Genetic variability by population
Genetic variability was also investigated in a subset of 185 Algerian and 70 Egyptian samples, for which population information was available (see “Materials and methods” section). As already presented for the total sample and for the dataset arranged in five Algerian and six Egyptian regions, the locus VOLP32 was the most informative (highest FST value, 0.09) (S3 Table), and the loci CVRL8 and CVRL1D displayed the lowest (2) and the highest (18–22) number of alleles also within the six populations considered in this study (S5 Table). No remarkable difference was observed between populations in allelic richness values, that ranged from 5.0 (Azawad) to 5.4 (Targui and Maghraby). Private allelic richness ranged from 0.21 (Rguibi) to 0.55 (Maghraby) (data not shown). The highest gene diversity was observed in the Sudani sample from Egypt (0.66 ±0.15), the lowest being observed in the Rguibi sample from Algeria (0.63±0.19) (S5 Table). The highest number of significantly (P<0.05) deviating loci (7) was observed for the Targui sample (S5 Table). Locus by locus FST values for the dataset arranged into different populations displayed a 0.94 correlation coefficient with locus by locus FST values for the dataset arranged into different geographic regions (data not shown).
Genetic relationship among regions
We first investigated genetic relationship among the considered eleven Algerian and Egyptian geographical regions by pair-wise FST (S6 Table). Out of 55 pair-wise comparisons, only 36 (65%) were significant with P<0.01 and differentiation values were generally low. The highest values were observed for the pair-wise comparisons involving samples from Al Qalaj (Egypt), all of them belonging to the Falahy population. Generally low values (0.002–0.012) were observed for pair-wise comparisons involving samples from different Algerian geographic regions, and only two of them (Steppe vs. Adrar and Steppe vs. Tamanrasset) were statistically significant. The network constructed using the distance of Reynolds et al. (1983) grossly mirrored the above results, with Algerian samples displaying a less expanded topology compared to the Egyptian ones, and a clear, though weak, differentiation between samples from the two countries being evident (Fig 2). We then investigated genetic structure using the unsupervised Bayesian clustering approach implemented in the software STRUCTURE. Based on the Evanno’s DeltaK statistics, the most probable number of clusters in our sample was K = 2 (S1 Fig). However, no clearly interpretable sub-structuring was observed at K = 2, nor at higher K values. Indeed, with the exception of the weak differentiation between Algerian and Egyptian samples, the other visible substructures could not be correlated to any geographic sampling area, population, or herd (Fig 3).
Genetic relationship among populations
In order to test whether the faint differentiation observed between samples from the two countries could be ascribed to differences among camel populations, we estimated pair-wise FST values among the three Egyptian (Falahy, Maghraby, Sudany) and the three Algerian (Azawad, Rguibi, Targui) populations. Although being very low, almost all the pair-wise FST values were significant (P<0.01). Again, differentiation between Egyptian population pairs was higher than Algerian population pairs (S7 Table). Similar results were also evident from the networks constructed using the distance of Nei (1983) and the distance of Reynolds et al. (1983) on the dataset arranged into the three Egyptian and the three Algerian considered populations (S2 and S3 Figs, respectively). The latter also displayed an intermediate position of Maghraby between Algerian and the other two Egyptian populations. The observed network topology (differentiation between Algerian and Egyptian samples, and intermediate position of the Maghraby population between Algerian and the other two Egyptian populations) was strongly supported by NJ tree bootstrap values (S4 and S5 Figs). Both NJ trees also highlighted the pairs Falahi-Sudani and Targui-Azawad. Results from COANCESTRY indicated that sampling was not biased towards highly related individuals. Mean relatedness (R) over the total sample was 0.058 ± 0.077. Within single populations, the majority (96% to 97%) of the pair-wise comparisons among individuals gave TrioML values lower than 0.25 (Unrelated animals), only a limited proportion of pair-wise comparisons (3% to 4%) gave TrioML values > 0.25 and≤0.5 (Half-siblings), and no pair-wise comparison produced TrioML values higher than 0.5 (Full-siblings) (S8 Table). Even more so, when estimating relatedness among individuals from different population pairs, the majority (96% to 98%) of the pair-wise comparisons gave TrioML values lower than 0.25 (Unrelated animals) (S8 Table).
Inventory and characterization of animal genetic resources and monitoring populations for variability are widely recognized as fundamental steps for any breed conservation and improvement programme . Unfortunately, advancements in this field are still scarce for the dromedary (Camelus dromedarius) species for which no long-established herd book breed exists and most of the existing populations have not been described yet or they have been only described at the phenotypic level. Compared to major livestock species, a reduced number of studies have been carried out on the genetic characterization of dromedary populations, mainly using microsatellite markers, and generally not trespassing single country sampling areas [45–59]. Very recently, the comprehensive study by Almathen et al. (2016) investigated dynamics of dromedary domestication and cross-continental dispersal combining ancient DNA sequences of wild and early-domesticated dromedary samples from arid regions with nuclear microsatellite and mitochondrial genotype information from 1,083 extant animals collected across the whole species’ geographic range. The study included samples from Algeria (30) and Egypt (30). However, the focus of the work being on cross-continental dispersal, the analyses were carried out on data aggregated into five defined geographical regions: Eastern Africa, Western and Northern Africa, North Arabian Peninsula South Arabian Peninsula, and Southern Asia including Australia. In this study, a survey of the genetic variability and relationships among six dromedary populations from thirteen geographic regions in Algeria and Egypt was carried out on a dataset of 331 animals by using 19 polymorphic microsatellite markers. A generally high level of polymorphism (number of alleles, gene diversity) was observed within the six considered populations, comparable with previous results from five Moroccan populations  and three Tunisian populations .
Genetic structure of camels in Algeria largely reflects past geographic distribution of nomadic pastoralist tribes
Consistent with a phylogeographic pattern, a slightly closer relationship of the Steppe region with Bechar and Tindouf (Moroccan border) than with Adrar and Tamanrasset (Mali and Niger border, respectively) was observed for the Algerian samples. This result is also in agreement with distribution of Rguibi dromedaries in the western part of Algeria, close to Morocco. Indeed, Rguibi dromedaries were originally reared by the Reguibat tribe, whose traditional vast nomadic range included, among other, also Morocco, and western Algeria . On the contrary, both Azawad and Targui dromedaries were originally reared by nomadic Touareg people that occupied a vast area including eastern Mali, western Niger, and south-eastern Algeria. Azawad dromedaries, that derive their name from the Azawad territory in northern Mali, are mainly present in Algeria in the wilaya of Adrar (whose southern territory borders Mali). Targui dromedaries, that derive their name from the Touareg people (in Arabic, Targui is the singular form of Touareg), in Algeria are mainly present in the Tamanrasset wilaya (whose southern territory borders Niger).
Egyptian camel populations: at the intersection between north-west and eastern Africa
In Egypt, four main camel populations are generally recognized, Sudani, Maghrabi, Falahi (or Fellahi, or Baladi) and Mowalled . The latter is a cross between the Maghrabi and the Falahi, and was hence not considered in our study. Among the three considered Egyptian populations, the Maghrabi resulted to be the most differentiated. These results are likely due to its geographical origin from coastal North-West Africa (Maghreb countries), while both Sudani and Falahi would have Eastern Africa origins. The Sudani is indeed imported from Sudan. The Falahi is bred in Upper Egypt but mostly used in the Nile delta region. A study carried out by  using few microsatellite markers highlighted a certain level of genetic relationship between Falahi and Somali dromedaries. As predictable based on geography, an intermediate position of Maghraby dromedaries, between the three Algerian and the other two Egyptian population clusters was observed in our study.
Weak genetic structure at the cross-border level in northern African dromedary camels
The faint sub-structuring observed using the unsupervised Bayesian clustering algorithm when analyzing the whole dataset is seemingly the result of a poor genetic differentiation, also suggested by the low observed FST values. In addition, while samples were a priori assigned to a specific population/geographic isolate when performing tree/network analysis, this was not the case when the Bayesian clustering approach was adopted (animals were allocated to assumed clusters by the algorithm). This could explain why a certain degree of differentiation was observable from trees and neighbor-network plots and not from the STRUCTURE plot. Indeed, when neighbor-network plots were obtained using inter-individual distance matrices (data not shown), instead of inter-population distance matrices, the obtained topologies did not suggest any interpretable scenario.
In summary, our results point to a weak genetic differentiation of dromedary populations and geographic isolates in two non-contiguous North African countries (Algeria and Egypt). These results are in agreement with those recently presented in the study by Almathen et al. (2016), where little population structure is observed using microsatellite loci in modern dromedaries, as a consequence of historical use as a cross-continental beast of burden along trans-Saharan caravan routes, coupled to traditional extensive/nomadic herding practices. A weak genetic differentiation has been observed also in the preliminary study by Ciani et al, performed using genome-wide SNP markers genotyped by double digest Restriction Associated DNA sequencing (ddRADseq), on African and Asiatic dromedaries. Together with the recent evidence for a low SNP density in dromedary genomes compared to genomes of domestic Bactrian, and even compared to genomes of the very small residual population of wild Camelus ferus animals , likely due to successive climate-driven demographic bottlenecks in the wild progenitor of Camelus dromedarius, this lack of genetic structure deserves close attention in view of the ongoing intensification process in the camel farming system  and the growing request for application of modern genetic improvement (bio)technologies. Notably, schemes of genomic selection across multiple populations are likely to represent the future choice for this species, in order to allow larger reference populations to be available (thus accounting for possibly lower linkage disequilibrium levels in dromedaries compared to other livestock species), and to take advantage from implementation of cross-border cost-sharing strategies.
S1 Table. Sample information.
S2 Table. Microsatellite information.
S3 Table. Locus-by-locus genetic diversity parameters for the total sample (N = 331).
Locus-by-locus genetic diversity parameters for the Algerian sample arranged into five geographical regions (A) and for the Egyptian sample arranged into six geographical regions (B).
Locus-by-locus genetic diversity parameters for the Algerian (A) and the Egyptian (B) samples arranged into six different populations.
S6 Table. Pair-wise FST values among the six Egyptian (Al Qalaj, Iking Maryut, Birqash, Marsa Matruh, Sidi Barrany, Negeila) and the five Algerian (Bechar, Steppe, Tindouf, Adrar, Tamanrasset) considered geographical regions.
S7 Table. Pair-wise FST values among the three Egyptian (Falahy, Maghraby, Sudany) and the three Algerian (Azawad, Rguibi, Targui) populations.
S8 Table. Proportions of pair-wise comparisons among individuals falling within the considered classes of relatedness (R).
S1 Fig. Plot of the DeltaK statistics (Evanno et al., 2005) for the total sample.
S2 Fig. Neighbor-net network constructed using the distance of Nei (1983) considering the whole dataset arranged into three Algerian (light blue area) and three Egyptian (light yellow area) populations.
S3 Fig. Neighbor-net network constructed using the distance of Reynolds et al. (1983) considering the whole dataset arranged into three Algerian (light blue area) and three Egyptian (light yellow area) populations.
S4 Fig. Neighbor-joining tree constructed using the distance of Nei (1983) considering the whole dataset arranged into three Algerian and three Egyptian populations.
S5 Fig. Neighbor-joining tree constructed using the distance of Reynolds et al. (1983) considering the whole dataset arranged into three Algerian and three Egyptian populations.
S1 Dataset. Genotypic data for the total population sample typed at 19 microsatellite loci.
We dedicate this work to the memory of Prof. Salah Galal, who contributed significantly throughout his career to the study of local animal genetic resources in Egypt. He closely collaborated with FAO, among other, in the establishment of the FAO Strategy for the Management of Animal Genetic Resources and the production of The State of the World Animal Resources for Food and Agriculture Report, presented at Interlaken, Switzerland in 2007. He has been Co-Editor of the FAO Animal Genetic Resources Bulletin and Associate Editor for the Animal Breeding and Genetics section of the Elsevier Small Ruminant Research journal. Prof. Galal published more than 100 papers in peer reviewed national and international journals and inspired a generation of scientists through his personality. We are grateful to the owners and collectors of the camel samples included in this study for their collaboration. We also would like to thank Ahmed Elbeltagi and Saif Agha for support in sampling.
- Conceptualization: YAC SBSG EC.
- Data curation: RG.
- Formal analysis: YAC EC.
- Funding acquisition: SBSG KAEB AMAA GML NSM.
- Investigation: YAC SBSG AMAA AAS DH EC.
- Methodology: EC SBSG.
- Project administration: SBSG GML NSM EC.
- Resources: YAC SBSG KAEB AMAA AAS DH GML NSM EC.
- Supervision: SBSG EC.
- Validation: YAC EC.
- Visualization: YAC RG.
- Writing – original draft: YAC EC.
- Writing – review & editing: YAC SBSG EC.
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