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Model Linking Plasma and Intracellular Tenofovir/Emtricitabine with Deoxynucleoside Triphosphates

  • Xinhui Chen,

    Affiliation University of Colorado, Skaggs School of Pharmacy and Pharmaceutical Sciences, Aurora, CO, United States of America

    ORCID http://orcid.org/0000-0001-5022-3323

  • Sharon M. Seifert,

    Affiliation University of Colorado, Skaggs School of Pharmacy and Pharmaceutical Sciences, Aurora, CO, United States of America

  • Jose R. Castillo-Mancilla,

    Affiliation University of Colorado, School of Medicine, Division of Infectious Diseases, Aurora, CO, United States of America

  • Lane R. Bushman,

    Affiliation University of Colorado, Skaggs School of Pharmacy and Pharmaceutical Sciences, Aurora, CO, United States of America

  • Jia-Hua Zheng,

    Affiliation University of Colorado, Skaggs School of Pharmacy and Pharmaceutical Sciences, Aurora, CO, United States of America

  • Jennifer J. Kiser,

    Affiliation University of Colorado, Skaggs School of Pharmacy and Pharmaceutical Sciences, Aurora, CO, United States of America

  • Samantha MaWhinney,

    Affiliation University of Colorado, Colorado School of Public Health, Department of Biostatistics and Informatics, Aurora, CO, United States of America

  • Peter L. Anderson

    Peter.Anderson@ucdenver.edu

    Affiliation University of Colorado, Skaggs School of Pharmacy and Pharmaceutical Sciences, Aurora, CO, United States of America

Model Linking Plasma and Intracellular Tenofovir/Emtricitabine with Deoxynucleoside Triphosphates

  • Xinhui Chen, 
  • Sharon M. Seifert, 
  • Jose R. Castillo-Mancilla, 
  • Lane R. Bushman, 
  • Jia-Hua Zheng, 
  • Jennifer J. Kiser, 
  • Samantha MaWhinney, 
  • Peter L. Anderson
PLOS
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Abstract

The coformulation of the nucleos(t)ide analogs (NA) tenofovir (TFV) disoproxil fumarate (TDF) and emtricitabine (FTC) is approved for HIV-infection treatment and prevention. Plasma TFV and FTC undergo complicated hybrid processes to form, accumulate, and retain as their active intracellular anabolites: TFV-diphosphate (TFV-DP) and FTC-triphosphate (FTC-TP). Such complexities manifest in nonlinear intracellular pharmacokinetics (PK). In target cells, TFV-DP/FTC-TP compete with endogenous deoxynucleoside triphosphates (dNTP) at the active site of HIV reverse transcriptase, underscoring the importance of analog:dNTP ratios for antiviral efficacy. However, NA such as TFV and FTC have the potential to disturb the dNTP pool, which could augment or reduce their efficacies. We conducted a pharmacokinetics-pharmacodynamics (PKPD) study among forty subjects receiving daily TDF/FTC (300 mg/200 mg) from the first-dose to pharmacological intracellular steady-state (30 days). TFV/FTC in plasma, TFV-DP/FTC-TP and dNTPs in peripheral blood mononuclear cells (PBMC) were quantified using validated LC/MS/MS methodologies. Concentration-time data were analyzed using nonlinear mixed effects modeling (NONMEM). Formations and the accumulation of intracellular TFV-DP/FTC-TP was driven by plasma TFV/FTC, which was described by a hybrid of first-order formation and saturation. An indirect response link model described the interplay between TFV-DP/FTC-TP and the dNTP pool change. The EC50 (interindividual variability, (%CV)) of TFV-DP and FTC-TP on the inhibition of deoxyadenosine triphosphate (dATP) and deoxycytidine triphosphate (dCTP) production were 1020 fmol/106 cells (130%) and 44.4 pmol/106 cells (82.5%), resulting in (90% prediction interval) 11% (0.45%, 53%) and 14% (2.6%, 35%) reductions. Model simulations of analog:dNTP molar ratios using IPERGAY dosing suggested that FTC significantly contributes to the protective effect of preexposure prophylaxis (PrEP). Simulation-based intracellular operational multiple dosing half-lives of TFV-DP and FTC-TP were 6.7 days and 33 hours. This model described the formation of intracellular TFV-DP/FTC-TP and the interaction with dNTPs, and can be used to simulate analog:dNTP time course for various dosing strategies.

Introduction

Tenofovir (TFV) disoproxil fumarate (TDF) and emtricitabine (FTC) are co-formulated as Truvada®, which is approved for human immunodeficiency virus 1 (HIV-1) infection treatment as part of combination antiretroviral therapy, as well as pre-exposure prophylaxis [1]. TDF is a prodrug, which undergoes ester hydrolysis on first pass by the gut and the liver and circulates in plasma as TFV predominantly [2, 3]. TFV and FTC are nucleos(t)ide analogs (NA) of deoxyadenosine monophosphate (dAMP) and deoxycytidine (dC). Each undergoes cellular uptake and anabolism to their active intracellular forms: TFV-diphosphate (TFV-DP) and FTC-triphosphate (FTC-TP) [4]. TFV-DP and FTC-TP compete with corresponding endogenous deoxynucleoside triphosphates (dNTP) at the active site of HIV reverse transcriptase (RT), thus inhibiting genetic material biosynthesis. If incorporated into the proviral DNA, TFV-DP and FTC-TP terminate chain elongation [3].

The accumulation of intracellular (IC) TFV-DP/FTC-TP is presumably driven by plasma TFV/FTC concentrations. However, the formation of intracellular TFV-DP/FTC-TP is complicated, as it requires a hybrid of endocytosis, active transport, diffusion, and enzymatic reactions. [2, 5]. These complexities contribute to nonlinearities in the pharmacokinetics (PK) relationship between plasma and intracellular TFV-DP [57]. Currently, many studies have characterized the PK of plasma TFV/FTC [815] and intracellular TFV-DP/FTC-TP [1622], individually. However, only a few PK link models have investigated the relationship between the plasma TFV and the intracellular TFV-DP, all of which used steady-state observations only. As a result, these models were restricted by the lack of accumulation phase data [2225].

In target cells such as CD4 T-cells, TFV-DP and FTC-TP compete with corresponding natural substrates of HIV RT, which are deoxyadenosine triphosphate (dATP) and deoxycytidine triphosphate (dCTP), respectively. The analog:dNTP molar ratios are associated with antiviral efficacies [19, 23]. However, as NAs, TFV and FTC also have the potential to disturb the dNTP pool, which consists of dATP, dCTP, deoxyguanosine triphosphate (dGTP), and thymidine triphosphate (TTP), given the interactions with the same enzymes in deoxypurine/deoxypyrimidine anabolic and metabolic pathways [2629].

We previously characterized the dNTP pool reductions during TDF/FTC therapy, in the same participants included in this communication [22, 30], and used non-compartmental analysis (NCA) to describe TFV-DP/FTC-TP concentration-time profiles [22]. However, there is a need to understand the pharmacokinetic-pharmacodynamic (PKPD) relationships and to model non-linearities between plasma and intracellular TFV/FTC along with dNTP changes [19, 20, 31].

Creating such models will enable PK-PD simulations of alternative dosing strategies and interpretations for various clinical scenarios. For example, nonlinear PK introduces difficulties in calculating clinical relevant half-lives via traditional methods. Sahin et al proposed the operational multiple dosing half-life, which is based on simulations of different dosing intervals (tau) [32]. In addition, for PK-PD simulations can be applied to alternative TDF/FTC dosing strategies such as on-demand preexposure prophylaxis (PrEP) studied by Molina et al, who reported an 86% protective rate against HIV-infection using IPERGAY dosing strategy (oral administration of two pre-coitus doses followed by two post-coitus doses) [33]. With dNTP data, PK-PD simulation can use target in vitro EC50 and EC90 for analog:dNTP ratio such as those proposed by Cottrell [31].

The main objective of this study was to develop a PK-PD model that characterized the nonlinearities of TFV-DP and FTC-TP using integrated compartmental PK models linking plasma TFV/FTC with intracellular TFV-DP/FTC-TP concentrations,including changes in dNTPs. The model was then used to estimate operational multiple dosing half-lives (t1/2.op) [32] and to simulate the analog:dNTP time profiles for the preexposure prophylaxis (PrEP) IPERGAY dosing [33].

Materials and Methods

Study design

This study was a prospective, observational, intensive, PKPD study in HIV-positive and negative adults, conducted at the University of Colorado Anschutz Medical Campus. The study was approved by the Colorado Multiple Institutional Review Board (COMIRB), and participants provided written informed consent before participation (Cell-PrEP; protocol in S2 File; ClinicalTrials.gov Identifier: NCT01040091; study duration: 03/2010–08/2013). HIV-negative subjects received daily oral co-formulated TDF 300 mg (TFV 136 mg) and FTC 200 mg for 30 days and HIV-positive subjects received daily co-formulated TDF 300 mg/FTC 200 mg plus efavirenz (EFV) 600 mg for 60 days. HIV-negative subjects had washout visits on days 35, 45, and 60 and HIV-positive subjects had one follow-up visit on day 60 (therapy was continued in this group). All participants had their baseline sample taken before the initiation of treatment for dNTPs. Subjects were asked to fast overnight, beginning at 10 pm before their dosing visits. On days 1 (first-dose) and 30, blood was collected at 1, 2, 4, 8, and 24 hours post-dose, and on days 3, 7, and 20, blood was collected pre-dose, 2 and 8 hours post-dose. Single blood samples were collected on days 35, 45, and 60 [22, 30]. The sample size was chosen to detect a ~60% increase (63%) in TFV-DP in HIV-negative versus HIV-infected persons using a significance level of 0.05, a power of 80%, a coefficient of variation for TFV-DP of 50%, and a two-sample t-test. This outcome was assessed in another communication [22].

Bioanalysis

Samples included plasma and peripheral blood mononuclear cells (PBMC). Blood was collected in EDTA tubes for plasma separation. CPT vacutainers were used to isolate PBMC, which were counted with an automated countess® cytometer (InvitrogenTM, Thermo Fisher Scientific Corporation, Carlsbad, CA) and lysed [22, 34]. TFV and FTC in blood plasma were assayed using a validated LC/MS/MS methodology with a lower limit of quantification (LLOQ) of 10 ng/mL [35]. TFV-DP and FTC-TP concentrations in cellular lysates were assayed with a validated LC/MS/MS method with an LLOQ of 2.5 fmol/sample for TFV-DP and 0.1 pmol/sample for FTC-TP [34]. The components of the dNTP pool (dATP, dCTP, dGTP, and TTP) were analyzed using a validated LC/MS/MS methodology, with an LLOQ of 50 fmol/sample [36]. For PBMC samples, values were converted to femtomole per million cells (fmol/106 cells, based on the number of cells per sample). The dNTP pool components were quantified in PBMC samples from baseline, 1, 2, 4, 8, 24 hours post-dose on day 1, and 8 hours post-dose in all other visits. TFV/FTC in plasma and TFV-DP/FTC-TP in PBMC were assayed at all time points [22, 30].

Model development

Concentration-time data were analyzed using the first-order conditional estimation with interaction (FOCEI) method of the nonlinear mixed effects modeling, NONMEM® (version 7.3, ICON plc., Ellicott City, MD, USA). Below limit of quantitation (BLQ) samples were treated as missing [37, 38]. Most BLQ samples for TFV-DP/FTC-TP were in the washout phase; for TFV-DP, 11 at day 60 and one at day 45; for FTC-TP, 19 at day 60, four at day 45; and one at day 35. On occasion, TFV-DP was BLQ within the first dose (five occasions), but other samples in the first dose were quantifiable for the subject (five samples were collected per subject). Plasma was always BLQ in the washout phase (all time points except five FTC at day 35). To develop link models, simultaneous (parameters from two models estimated at the same time) and sequential link (parameters from one model were estimated in advance and fixed) methods were both investigated [39, 40]. The plasma TFV model was linked to the intracellular TFV-DP model, then TFV-DP model was linked to endogenous dATP and dGTP (deoxypurines) models, given TFV is a deoxypurine analog and could affect deoxypurine turnover. The FTC models were linked in a similar fashion except with endogenous dCTP and TTP (deoxypyrimidines), as FTC is a deoxypyrimidine analog and could affect deoxypyrimidine turnover.

Classical one-, two-, and three-compartmental models were investigated to develop plasma TFV/FTC models. The plasma models were then linked to the intracellular TFV-DP/FTC-TP model. Because units from the two models carried different meanings (ng/mL vs. fmol/106 cells), mass movement was avoided. Instead, plasma TFV/FTC concentrations (regarded as “exposure”) were used to stimulate TFV-DP/FTC-TP formation (regarded as “response”). Models tested included the first-order formation model described by Burns et al [24], the saturable formation model described by Duwal et al [6], the indirect response model described by Baheti et al [7], and the saturation (tolerance) model described by Porchet et al [41]. One-, two-, and three-compartmental models were investigated to describe the elimination phase of TFV-DP/FTC-TP.

TFV-DP/FTC-TP concentrations were then linked to the endogenous dNTP pool change (response). The concentrations of TFV-DP/FTC-TP were used to inhibit the zero-order rate constant of the corresponding dNTP production (k0in). Direct link and indirect response link, as well as Emax models and sigmoidal Emax models (hill coefficient), were investigated [42, 43]. The baseline response (dNTP at time zero, R0) is the determinant of indirect responses and a source of variation in pharmacodynamics analysis [44]. Thus, we assessed the three different estimation methods as described by Dansirikul et al [45] for the endogenous dNTP pool R0 that arose from a single measurement. These were method 1: estimation using deviation (ω2) from the population mean baseline values, method 2: estimation using deviation (σ2) from the individual observed baseline values, and method 3: estimation using both weighted population mean and individual observed baseline values.

Several random effect models were also evaluated to describe interindividual variability (IIV) and residual variability (RV), such as additive, proportional, and exponential models. To assess the model goodness of fit, the objective function value (OFV) was used, with a lower value indicating a better fit.

Acceptable models were selected based on the following criteria: successful minimization; successful covariate step; absolute value of gradients less than 1 in the last iteration; the %RSE (relative standard error) less than 30% for fixed effects in base model, while %RSE less than 50% for random effects; 95% confidence intervals for fixed and random effects not including 0; model conditional number (generated in covariate step) not exceeding 10p (p: number of parameters); no significant correlation among parameters (r<0.8); and eta bars not statistically significant from zero (p>0.05).

Covariate selection

The effects of covariates on the model parameters were evaluated. A linear model was used for categorical variables such as HIV-infection status, sex, and race. For continuous variables such as estimated glomerular filtration rate (eGFR), age, weight, and body mass index (BMI), subject variables were centered at medians. Then, linear, natural log, and power models were evaluated [46]. The covariates were assessed by likelihood ratio test (LRT), with a forward selection at α = 0.01 (OFV decreased by 6.64), then a backward elimination at α = 0.001 (OFV increased by 10.8).

Model evaluation

The goodness of fit diagnostics was generated and visually assessed using Xpose [47] (version 4.5.3, http://xpose.sourceforge.net/) and R (http://www.r-project.org/), which includes plots of observations vs. population predictions, observations vs. individual predictions, absolute values of individual weighted residuals vs. individual predictions, and weighted residuals vs. time (basic diagnostic plots). No substantial pattern was allowed in these diagnostic plots. In addition, an overlay plot of observed values and predicted values vs. time was also used to assess the model performance.

Visual predictive checks (VPC) were performed using NONMEM–PDxPOP (version 5.1, ICON plc., Ellicott City, MD, USA) and R. VPCs were conducted using 100× study population (n = 4000) as the sample size. VPCs of the first-dose (day 1), as well as day 1–60 in the HIV-negative group (to capture washout) for intracellular models (TFV-DP, FTC-TP, dATP, dCTP, dGTP, and TTP), were generated. An overlay plot of observed values as well as 90% percentiles, 80% percentiles, and medians were generated and evaluated. Observations outside 90% confidence intervals were also calculated, and a proportion of less than 15% was considered acceptable.

Model evaluation by bootstrapping (n = 1000) was carried out with PDxPOP and R. The 95% percentiles (2.5% and 97.5%) and the bootstrapping success rates were generated and assessed. A success rate that exceeded 90% was required for an acceptable model.

Simulations and model applications

To demonstrate the application of the model, simulations were carried out for the calculation of operational multiple dosing half-lives (t1/2,op) and the characterization of the change of analog:dNTP molar ratios over time for on-demand PrEP purpose using the IPERGAY doing strategy [32, 33].

Given the nonlinearities of TFV-DP/FTC-TP disposition (see results), simulations were performed to calculate the intracellular PK (TFV-DP/FTC-TP) operational multiple dosing half-lives (t1/2,op), proposed by Sahin et al [32]. Simulations of population mean concentrations were performed. For TFV-DP, simulations of different dosing intervals (tau) from 1 day to 9 days (graduated by 1 day) were performed. Dosing intervals of 0.2, 0.5, 1, 1.5, 2, and 2.5 days were performed for FTC-TP. A total of 50 doses were simulated to ensure the acquisition of steady-state. Maximum concentrations from the first-dose (Cmax,fd), maximum concentrations at steady-state (Cmax,ss), and minimum concentrations at steady-state (Cmin,ss) were then determined for the different tau values. The operational half-lives were defined as the tau where the Cmax,ss:Cmax,fd = 2 (suggesting the dosing interval achieved doubled concentrations at steady-state compared with first-dose), as well as the Cmax,ss:Cmin,ss = 2 (suggesting the dosing interval led to 50% elimination at trough compared with peak concentrations at steady-state).

For the IPERGAY trial design for on demand PrEP dosing strategy, Monte-Carlo simulations (n = 1000) were performed. The IPERGAY dosing consisted of two doses (600/400 mg TDF/FTC), 24 hours to 2 hours before coitus, followed by one dose (300/200 mg TDF/FTC) at 24 hours after coitus and another dose at 48 hours post-coitus. Statistical analysis of simulation results was carried out using SAS® (version 9.4, SAS Institute Inc., Cary, NC, USA). Simulations of dosing relative to a coitus event in a virtual HIV-negative population were set for a time course of one-week (7 days), and data generated in every 0.1 days post-dose, as well as at 10−4, 10−3 and 10−2 days after the first-dose (to capture the initial accumulation). Medians and percentiles (5th percentile (P_5) to 95th percentile (P_95), graduated by 5) of molar ratios of TFV-DP:dATP and FTC-TP:dCTP were calculated, then compared with the CD4 T-cell viral suppression EC50 and EC90 (molar ratio) in vitro, reported by Cottrell et al [31].

Results

Study demographics

21 HIV-negative adults and nineteen HIV-positive patients (n = 40) were enrolled in the study. Thirteen participants were female, and 27 were male. Nineteen were Caucasians, 16 were African Americans, and five were Hispanic. The median (range) age was 31 (20–52) years. The median eGFR (range) was 93.3 (66.0–131) mL/min/1.73m2. The medians (ranges) weight and BMI were 81.1 (56.5–127) kg and 26.6 (19.9–37.7) kg/m2, respectively. The clinical trial flow chart is shown in Fig 1. TDF and FTC were well tolerated throughout the study (see Fig 1). 34 (among 40) subjects completed all study visits, two HIV negative and four HIV-positive subjects stopped the study early [22, 30], but all available longitudinal data prior to dropout were included in the model development.

Population pharmacokinetics modeling of plasma TFV/FTC

The best fit was obtained using a classical first-order absorption two-compartmental model (subroutines ADVAN4 TRANS4) for both plasma TFV and FTC. Model structures are illustrated in Fig 2 left portion (compartment 1–3). Differential equations of the plasma model are listed in Equations A-C in S1 File. In these models, bioavailability (F) adjusted central compartment volume of distribution (Vc/F), clearance (CL/F), intercompartmental clearance (Q/F), and peripheral compartment volume of distribution (Vp/F) were estimated. The absorption rate constant (Ka) was estimated, but a lag time (tlag) was not.

thumbnail
Fig 2. Model schematic.

Red box represents plasma. Blue box represents cells. Solid outlines suggest observations available in these compartments. Dashed outlines indicate virtual compartments. Dashed arrows indicate where mass movements were avoided during model development. PBMC: peripheral blood mononuclear cells. IC: intracellular TFV: tenofovir. FTC: emtricitabine. TFV-DP: tenofovir diphosphate. FTC-TP: emtricitabine triphosphate. dATP: deoxyadenosine triphosphate. dGTP: deoxyguanosine triphosphate. dCTP: deoxycytidine triphosphate. TTP: thymidine triphosphate. F: bioavailability. Ka: absorption rate constant. Q: intercompartmental clearance. Vc: central compartment volume of distribution. Vp: peripheral compartment volume of distribution. Kf: first-order formation rate constant of intracellular TFV-DP/FTC-TP stimulated by plasma TFV/FTC. SC50: concentration in a virtual saturation compartment at which formation rate was inhibited by 50%. R: recycling portion constant in %. Kel: elimination rate constant of intracellular TFV-DP/FTC-TP. K0in: zero-order production rate constant of intracellular dNTPs. Kout: elimination rate constant for intracellular dNTPs. EC50: concentration of intracellular TFV-DP/FTC-TP at which K0in is inhibited by 50%.

https://doi.org/10.1371/journal.pone.0165505.g002

Exponential random effect model on interindividual variability (IIV) and residual variability (RV) resulted in lowest OFV. The IIV for the Vc/F, CL/F, and Q/F were estimated in the TFV plasma model. In the FTC plasma model, the IIV in Vc/F, CL/F, and the Vp/F were estimated. The model estimated terminal half-lives (beta half-life, t1/2,z) of TFV and FTC were 17.3 hours (95%CI: 15.7, 19.1) and 26.8 hours (95%CI: 25.6, 28.1). In the FTC plasma model, sex was statistically significant (p = 0.009; ΔOFV = -6.76; ΔIIVVc/F = -2.8%), as males had 24.4% (95% CI: 7.25%, 41.6%) higher average (typical value, tv) central compartment volume of distribution (tvVc/F = 99.4+24.3×sex; male = 1, female = 0). No subject variable was statistically significant in the TFV plasma model. The goodness of fit plots demonstrated that the model provided a good description of the data (Figures A-C in S1 File). The VPCs are shown in Fig 3A and 3B upper left panels. Model parameters, as well as bootstrapping outcomes, are listed in Table 1. Other model evaluation results are shown in Table A in S1 File.

thumbnail
Fig 3. Models visual predictive check.

Circles indicate observations. Solid lines indicate model 90% prediction intervals. Dashed lines indicate model estimated medians and 80% prediction intervals. (A) TFV-deoxypurine models visual predictive check. The results of the first-dose inserted with the results of the 60 days in the HIV-negative group (had a washout period from day 30–60) for intracellular TFV-DP, dATP and dGTP are shown. TFV: tenofovir. TFV-DP: tenofovir diphosphate. dATP: deoxyadenosine triphosphate. dGTP: deoxyguanosine triphosphate. (B) FTC-deoxypyrimidine models visual predictive check. The results of the first-dose inserted with the results of the 60 days in the HIV-negative group (had a washout period from day 30–60) for intracellular FTC-TP, dCTP and TTP are shown. FTC: emtricitabine. FTC-TP: emtricitabine triphosphate. dCTP: deoxycytidine triphosphate. TTP: thymidine triphosphate.

https://doi.org/10.1371/journal.pone.0165505.g003

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Table 1. Model parameters.

IC: intracellular. (A) TFV-deoxypurine model parameters. (B) FTC-deoxypyrimidine model parameters.

https://doi.org/10.1371/journal.pone.0165505.t001

Population pharmacokinetics modeling of TFV-DP/FTC-TP

Individual PK parameters from the plasma models were fixed for the intracellular TFV-DP/FTC-TP model development (sequential link). Model structures are illustrated in Fig 2 upper right portion (compartment 4–6). Differential equations for intracellular TFV-DP/FTC-TP models are listed in Eqs 13.

(1)(2)(3)

A hybrid of first-order formation and saturation link model best described the data. In this model, a saturation compartment (compartment 5) was used, in which high plasma concentrations (A(2)/Vc) slowed the first order rate constants of TFV-DP/FTC-TP formation (Kf). The elimination rate constant in the saturation compartment was fixed to the plasma central compartment elimination rate constant (CL/Vc) to avoid overparameterization, assuming that the saturation effect disappeared based on plasma TFV/FTC disappearance. An SC50 was introduced in the saturation model, which indicated the concentration in the virtual saturation compartment that leads to 50% inhibition of TFV-DP/FTC-TP formation. The stimulation constant for both the saturation compartment and the TFV-DP/FTC-TP compartment (IC central, compartment 4) was estimated with the same rate constant of formation (Kf) to avoid overparameterization. A two-compartmental model best described TFV-DP and FTC-TP elimination. This was modeled with a “recycling” compartment (compartment 6). Due to the collinearity (r>0.9) between K46, K64 (rate of movement from compartment 4 to 6, 6 to 4), and K40 (elimination rate of compartment 4), a “recycling” ratio constant (%R, ranged from 0 to 1) was used. Given the high correlation, the intercompartmental distribution rate constants (K46 and K64) between the IC central (compartment 4) and the “recycle” compartment (compartment 6) were defined as R×Kel, and the IC central elimination rate constant (K40) defined as (1-R)×Kel. In effect, this modeling created a second elimination phase for TFV-DP and FTC-TP, consistent with a “recycling” (re-phosphorylation) or a “deep” (slow turnover) compartment.

The model used user-defined differential equations (subroutines ADVAN13 TRANS1). TFV-DP and FTC-TP shared the same model structure. The exponential random effect model on IIV and proportional random effect model on RV resulted in the lowest OFV. The IIV was estimated in Kf and Kel for IC central compartment in both TFV-DP and FTC-TP models. In the intracellular FTC-TP model, HIV infection status was found to be statistically significant in regard to the formation rate constant (tvKf = 41.6+31.3×HIV; positive = 1, negative = 0), as HIV-infected patients had 75.2% (95%CI: 35.6%, 115%) higher average formation rate (p<0.00001; ΔOFV = -20.5; ΔIIVKf = -5.6%). No statistically significant subject variable was observed in the TFV-DP model.

The alpha half-lives (ln2/λ1, where λ1 is the slope of the concentration decline in the initial phase) of TFV-DP and FTC-TP were 73.4 hours (95%CI: 61.8, 87.1) and 8.8 hours (95%CI: 8.2, 9.4). The beta half-lives (associated with compartment 6, t1/2,z, ln2/λ2, where λ2 is the slope of the concentration decline in the terminal phase) were 55.6 days (95%CI: 46.9, 66.0) and 76.8 hours (95%CI: 71.7, 82.2). However, these beta half-lives only represented a small fraction of concentration-time profiles, as the “recycling” ratios for the description of the second elimination phase were only 5.82% and 16.0% (see Table 1A and 1B). The goodness of fit plots showed good descriptions of the data (Figures A, D and E in S1 File). The VPCs are illustrated in Fig 3A and 3B upper right panels. Model parameters and bootstrapping outcomes are listed in Table 1A and 1B middle portions. Other model evaluation results are shown in Table A in S1 File.

Population pharmacodynamics modeling of dATP/dGTP and dCTP/TTP

To develop dNTP models, individual PK parameters of TFV-DP/FTC-TP models were first fixed (sequential link). In the previous study of this data, dNTPs were shown to decrease during TDF/FTC treatment [30]. Thus, intracellular TFV-DP/FTC-TP models were linked to the reduction of the zero-order production rate constant (K0in) of the corresponding intracellular endogenous dNTPs. The indirect response Emax model best described the data. An EC50 was introduced in the model, which indicated concentrations of TFV-DP/FTC-TP that led to 50% inhibition of the corresponding dNTP production, and the typical values were estimated as tvEC50 = EXP(THETA) to improve precision and to ensure positive estimations. Model structures are shown in Fig 2 lower right portion, as response compartment (compartment 7). The equation was (Eq 4): (4)

To avoid overparameterization, the elimination rate constant (Kout), as well as maximum drug effect (Emax) were fixed to 1. K0in and the baseline dNTP (R0) were interconvertible (K0in = R0×Kout). To estimate the initial individual status of response compartment (compartment 7), in other words, baseline level of dNTP, the model below was chosen (Eq 5). (5) Where R0i is the model estimated individual baseline level of dNTP. (R0)^ is the model estimated population mean level of dNTP. R0i,o is the observed individual dNTP level at baseline. ω is the standard deviation (sd) of model estimated IIV of dNTP level at baseline. σ is the sd of model estimated residual error of dNTP level throughout the observation period (0–60 days). ηi,RV represents the random effect of residual error (a normal distribution with mean = 0 and sd = σ). The effect on dGTP waned over time, and this was described using a sigmoidal E’max ~ time model. However, due to the small sample size and high data variability, this term was simplified to 1/(1+timeγ) (Equation D in S1 File, where γ is a power exponent that modifies the effect of time mathematically).

The best fit was obtained using the user defined model (subroutines ADVAN13 TRANS1). For dATP, dCTP, and TTP models, the IIV was estimable on R0 and EC50. For the dGTP model, the IIV was only estimable for R0. The exponential random effect model on IIV and RV achieved the lowest OFV. No subject covariate was found to be statistically significant. The goodness of fit plots showed a good description of the data (Figures A and F-I in S1 File). The VPCs are illustrated in Fig 3A and 3B lower panels. Model parameters and the bootstrapping 95% CIs are listed in Table 1A and 1B lower portions. The model estimated (median (5% and 95% percentile)) decreases were 11% (0.45%, 53%) in dATP; 14% (2.6%, 35%) in dCTP and 24% (4.5%, 62%) in TTP. The dGTP reached a low point around day 2.5 with a reduction of 13% (6.9%, 21%), then returned to the baseline value; other components in the dNTP pool returned to baseline during the washout period. Other model evaluation results are shown in Table A in S1 File.

Simulations and model applications

Calculation of operational multi-dosing half-lives.

Given nonlinear PK relationships between plasma TFV/FTC and intracellular TFV-DP/FTC-TP, an operational half-life (t1/2,op) was determined [32]. Simulations were performed to generate ratios (Cmax,ss:Cmax,fd and Cmax,ss:Cmin,ss) from different dosing intervals (tau). The curves generated from these ratios are illustrated in Fig 4. The nonlinearity of TFV-DP and FTC-TP accumulation resulted in the interception of the two curves at ratio≠2. The intercepts at x-axis where Cmax,ss:Cmax,fd = 2 and Cmax,ss:Cmin,ss = 2 were defined as the multiple dosing half-lives (t1/2,op). Based on Cmax,ss:Cmax,fd = 2, the t1/2,op of TFV-DP and FTC-TP were 4.2 days and 4.8 hours. Based on Cmax,ss:Cmin,ss = 2, the t1/2,op of TFV-DP and FTC-TP were 6.7 days and 33 hours.

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Fig 4. Intracellular operational multiple dosing half-lives.

Cmax,fd: maximum concentration at first -dose. Cmax,ss: maximum concentration at steady-state. Cmin,ss: minimum concentration at steady-state. TFV-DP: tenofovir diphosphate. FTC-TP: emtricitabine triphosphate. Y-axis: ratios. X-axis: dosing interval. Dashed lines in red represent the curve of Cmax,ss:Cmax,fd. Solid lines in blue represent the curve of Cmax,ss:Cmin,ss. X values at which dashed lines or solid lines intercept at y = 2 represent operational multiple dosing half-lives.

https://doi.org/10.1371/journal.pone.0165505.g004

Simulations of IPERGAY trial design.

Given the importance of analog:dNTP on NA efficacy, and findings of reduced dNTPs over time, simulations of medians and selected percentiles of analog:dNTP ratios for the IPERGAY trial design are illustrated in Fig 5.

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Fig 5. Protection with pre- and post-coital PrEP dosing.

Left panels illustrate TFV-DP:dATP molar ratios. Right panels illustrate FTC-TP:dCTP molar ratios. All simulations included another two doses at 24 hours and 48 hours after coitus. Upper panels illustrate 24 hours dosing pre-coitus. Lower panels illustrate 2 hours dosing pre-coitus. P_n: nth percentile. TFV-DP: tenofovir diphosphate. FTC-TP: emtricitabine triphosphate. dATP: deoxyadenosine triphosphate. dCTP: deoxycytidine triphosphate.

https://doi.org/10.1371/journal.pone.0165505.g005

When the dose was taken 24 hours pre-coitus, the model predicted that 85% of the virtual population had a TFV-DP:dATP molar ratio above EC50 (0.086) at the time of coitus and this persisted for 7 days. The percentage dropped to 50% for 2 hours pre-coital dosing. When evaluating using EC90, 20% of simulated individuals were above EC90 when dosing was 24 hours pre-coitus, but this dropped to 5% when the dose was taken 2 hours before coitus, illustrating the slow accumulation of TFV-DP.

At the time of coitus, virtually all individuals (>95%) had FTC-TP:dCTP above EC90 (0.07) and EC50 (0.022), and this persisted for at least seven days, regardless of the timing of the pre-coital dosing.

Discussion

The PK links between plasma TFV/FTC and intracellular TFV-DP/FTC-TP are complicated due to nonlinearities. The accumulation phases are driven by endocytosis, diffusion, and active transport of plasma TFV/FTC, as well as multiple kinases and phosphorylases that orchestrate the intracellular phosphorylation and dephosphorylation [2, 5]. Duwal et al [6], Baheti et al [7], and Burns et al [24] successfully described steady-state kinetics of TFV-DP/FTC-TP using a saturable formation model, an indirect response model, and a first-order formation model. However, all these models failed to minimize in our study, which included observations from first-dose to steady-state. The reason may be that plasma TFV/FTC fluctuates in roughly similar (little accumulation) and wide ranges, whereas intracellular TFV-DP/FTC-TP slowly accumulates. When plotting “exposure” (plasma TFV/FTC) vs “response” (intracellular TFV-DP/FTC-TP) from first-dose to steady-state, we observed “handshape” relationships (examples are shown in Figure J in S1 File) instead of “S-shape” relationships (suggesting direct effects) or hysteresis loops (suggesting indirect effects), similar to observations of Porchet et al for nicotine tolerance [41]. These “handshape” relationships suggested saturation of phosphate formation for high plasma concentrations, as previously described by Madrasi et al [5]. This effect hints at a constantly changing saturable formation rate of intracellular TFV-DP/FTC-TP by plasma TFV/FTC. We addressed this with a novel TDF/FTC PK model link method—a hybrid of first-order formation and saturation—to describe the formation of intracellular TFV-DP/FTC-TP. However, these models might be limited by the coadministration of TDF/FTC in our study, which limited the ability to assess potential interactions in vivo, as some in vitro studies suggest a metabolic interaction of intracellular TFV-DP and FTC-TP [48]. Studies with the individual drugs are needed to address potential intracellular interactions.

In addition, a possible “recycling” or “deep” compartment for TFV-DP/FTC-TP distribution was identified, manifesting as biphasic elimination. The complex elimination processes are driven by cell trafficking from peripheral tissue with peripheral blood, as well as multiple enzymes that phosphorylate/dephosphorylate nucleoside analogs [4952]. Triphosphates that are eliminated (dephosphorylated) may be re-phosphorylated. The biphasic elimination was detected during model development, as a one-compartmental model underpredicted the day 15–30 data in the washout period (day 45–60 of the overall study). As a result, a “recycle” compartment (IC peripheral, compartment 6) was introduced in our model. A three-compartmental model did not converge, due to limited sampling and overparameterization. A two-compartmental model (utilizing compartment 6) best described the data, assuming that part (%R, range from 0 to 1) of the eliminated intracellular TFV-DP/FTC-TP “recycled” (“re-phosphorylated”), or reappeared from a “deep” compartment back into the IC central compartment (compartment 4). However, this compartment was described via a mathematical approach, and the biological interpretation is limited by the study duration (30 doses in HIV-negative group), sparse washout period sampling (5, 15, and 30 days in washout in HIV-negative group only), and the possible influence of the treatment of samples that were below the limit of quantitation (BLQ) as missing, which is a loss of information reducing power and potentially biasing estimates for this portion of the curve [37,38]. We chose not to impute BLQ values (eg between 0 and the LLOQ) as this may also lead to bias [38]. Further study of this “deep” compartment is needed.

During model link development, simultaneous and sequential link methods were both assessed. Simultaneous link models did not minimize after >48 hours of computation (intel® Xeon® Processor E5-2637 v3 at 3.50 Ghz). Therefore, we chose the sequential link method to develop the model link, as these approaches show similar robustness [40].

These models detected two covariates that were statistically significant. In the plasma FTC model, the male participants had a higher volume of distribution of the central compartment (compartment 2) compared with female participants, which might be explained by the higher lean weight in males into which FTC distributes. In the intracellular FTC-TP model, HIV-positive individuals had a higher FTC-TP Kf but not Kel compared with HIV-negative individuals. Seifert et al reported that FTC-TP area under the concentration-time curve (AUC) was higher at the first-dose in HIV-infected patients (first-dose concentration is affected by Kf), but this effect disappeared at steady-state (presumably Kel is rate limiting at steady-state concentrations) [22]. Our study was limited by small sample size (n = 40) and sampling time, which did not allow us to estimate the IIV for some parameters (e.g. Ka and SC50) in the model and to detect statistically significant effects of subject covariates, particularly the HIV-infection status. Other studies suggest lower intracellular NA triphosphates in HIV-negative individuals, so further work is needed to better define such effects [53].

For plasma TFV/FTC model development, a classical two-compartmental model was used, similar to previous studies [6, 7, 11, 14, 15, 24, 25]. The TFV PK parameters were within the range of literature [7, 24, 25]. Compared with Valade et al [14, 15], FTC PK parameters were all higher (CL/F (L/day): 482 vs 362; Vc/F (L): 99.4 vs 42.3; Vp/F (L): 166 vs 55.4) except Q/F was similar (141 vs 138 (L/day)). Ka was reported to be difficult to estimate [6, 14, 15, 24, 25]. However, in our study, even with sampling starting at 1 and 2 hours (TFV tmax:0.5–3.0 hours, FTC tmax: 1.5–3.0 hours [2, 9, 5456]), Ka estimation was possible, although tlag was not [7, 11]. In bootstrapping analysis, extreme values in 95% CI were observed for a few parameters (Table 1A and 1B), including the upper bound of Ka_FTC, and the lower bounds of IIV for Vc/FTFV, Vp/FFTC and EC50_FTC-TP,dCTP. These results suggest possible limitations in the sampling times and sample size of our study. To assess the effect of parameter instabilities in bootstrapping analysis, sensitivity analyses were performed by fixing Ka_FTC = 55.7/day and not estimating the IIV for Vc/FTFV, Vp/FFTC and EC50_FTC-TP,dCTP. Similar model parameter estimations were obtained (%difference<27.6) and the general conclusions of these models remained.

We previously found reduced dNTPs in this study cohort but had not linked drug concentrations to these effects [30]. In this analysis, PKPD models linking the TFV-DP–dATP/dGTP and the FTC-TP–dCTP/TTP were developed using an indirect response model. Given the limited sample size and high variability in dNTPs, several PD parameters (Emax and Kout) were fixed to 1 to avoid overparameterization. Because the PD model heavily depended on the baseline response (initial status of compartment 7), to effectively estimate the baseline response, different baseline models were assessed as described by Dansirikul et al [45]. This approach incorporated both the population means and observed values into the estimation, which best described the data. Future studies should consider repeated measurements prior to treatment initiation to improve the estimation of baseline. In the TFV-DP–dGTP link model, a transient effect that reduced over time was observed [30]. This may represent purine nucleoside phosphorylase (PNP) inhibition by intracellular TFV phosphates which would theoretically raise dGTP [28]. However, a larger sample size is required to probe these responses of dGTP in the future.

The half-lives of TFV and FTC were within the range of previous studies [10, 12, 18, 5759] (TFV: 11.6–31 h; FTC: 3.0–40.5 h). The alpha half-life of TFV-DP (73 hours) was within the range of literature (53.3–125 hours[6, 7, 24]). The beta half-life (55.6 days) may be an overestimation as it was limited by the sparse sampling in washout phase and the approach of handling BLQ as missing values. However, it only accounted for a small portion (RTFV-DP = 5.8%) of the concentration change in the washout. Non-compartmental analysis (NCA) reported a half-life of 106 hours (TFV-DP) and 54 hours (FTC-TP) in these same HIV-negative subjects in the 30-day washout (n = 17) [60]. Others reported similar half-lives in prolonged observations in washout phase. For example, Hawkins et al, in their trial with a 28-day TFV-DP washout, reported a half-life of 150 h. Dickinson et al reported 116 h (TFV-DP) and 37 h (FTC-TP) half-lives in their 7-day washout observation[18]. Jackson et al reported 164 h (TFV-DP) and 39 h (FTC-TP) in their 10-day washout observation [12]. In a two-compartmental model, the apparent half-lives are the mixture of alpha and beta half-lives. Given this and the non-linearities, the simulation based operational multiple dosing half-lives (t1/2,op) might be informative.

Models enabled us to better investigate these complexities by applying the t1/2,op calculation proposed by Sahin et al [32]. They recommended generating both curves of Cmax,ss:Cmin,ss and Cmax,ss:Cmax,fd from different dosing intervals (tau), and showed that for linear systems, the two curves will intercept at ratio = 2 (such as plasma diazepam [32]). In our study, the interception did not occur at ratio = 2 due to the nonlinearities, resulting in two t1/2,op. Nonlinearities in accumulation favors a higher first-dose concentration (Cmax,fd), and a lower steady-state concentration (Cmax,ss), resulting in a lower position of the Cmax,ss:Cmax,fd curve. Results of t1/2,op calculated from Cmax,ss:Cmin,ss = 2 were comparable to current literature. The t1/2,op (161 hours) of intracellular TFV-DP supports the prolonged viral suppression for up to a week after the treatment discontinuation, reported by Deeks et al [61], and is also similar to the 150 hours reported by Hawkins et al [21] and 180 hours reported by Pruvost et al [62]. The t1/2,op for FTC-TP (33 hours) is also comparable to other literature values (37–39 hours) [12, 18, 59].

These models also enabled simulations of the analog:dNTP time courses for IPERGAY dosing [33]. Models estimated that two tablets pre-coitus plus two tablets post-coitus had 5–20% of >90% protective effect and 50–85% of >50% protective effect from TDF. The relatively slow accumulation of TFV-DP on day 1 may be a disadvantage of TDF for on demand PrEP, but the FTC-TP:dCTP ratios were well above EC90 after the first-dose. This suggests that FTC contributes significantly to the protective on demand PrEP effect of TDF/FTC and is consistent with 86% efficiency from IPERGAY. In these simulations, drug concentrations dominated the molar ratio (analog:dNTP) change, while the relatively small changes in dNTPs did not contribute much to the ratio change. This simulation is limited by the assumption of a simple/direct relationship between analog:dNTP and efficacy, and that the intracellular data were generated from PBMC only. TFV-DP/FTC-TP levels may differ among cell types, such as in tissue mononuclear cells in rectal and female genital tissues that are the major target for PrEP [31, 63]. The dNTP pool also varies among different cell types [31]. Thus, our simulation in PBMC might not represent the cells in target tissues. Nevertheless, PBMC includes CD4 T-cells, which is one of the major target cells for HIV and antiretroviral agents. Also, model development was based on oral dosing, thus simulations may not extrapolate to topical dosing scenarios, in which tissues, concentration gradients, and model structure are different, and other biological factors may be important to consider, such as local pH and the microbiome.

In conclusion, we developed PKPD models linking plasma and intracellular TFV/FTC with the dNTP pool. Observations from first-dose to steady-state were necessary for model development to describe complex accumulation processes. A hybrid of first-order formation and saturation was used to describe nonlinearities of intracellular TFV-DP/FTC-TP formation. An indirect response model was used to describe the interaction between TFV-DP/FTC-TP and dNTPs. These models can be helpful to understand the time profiles of analog:dNTP ratio changes in different clinical trial designs and to calculate the operational multiple dosing intracellular half-lives. Future study is required to investigate the possible mechanisms of these findings and to test other covariates.

Supporting Information

S1 File. Equations A-D: Supplement equations.

Table A: Other model evaluation results. Obs: observed values. Figure A: Dependent variables (observed values) and predicted values vs time plots. IC: intracellular. PRED: predicted values. DV: dependent variables. Figure B: Basic goodness of fit plots of TFV plasma model. Red line represents average values. Black line represents theoretical values. Data from the same individual are shown in blue circles and are connected by lines. |iWRES|: absolute values of individual weighted residuals. Figure C: Basic goodness of fit plots of FTC plasma model. Red line represents average values. Black line represents theoretical values. Data from the same individual are shown in blue circles and are connected by lines. |iWRES|: absolute values of individual weighted residuals. Figure D: Basic goodness of fit plots of intracellular TFV-DP model. Red line represents average values. Black line represents theoretical values. Data from the same individual are shown in blue circles and are connected by lines. |iWRES|: absolute values of individual weighted residuals. Figure E: Basic goodness of fit plots of intracellular FTC-TP model. Red line represents average values. Black line represents theoretical values. Data from the same individual are shown in blue circles and are connected by lines. |iWRES|: absolute values of individual weighted residuals. Figure F: Basic goodness of fit plots of intracellular dATP model. Red line represents average values. Black line represents theoretical values. Data from the same individual are shown in blue circles and are connected by lines. |iWRES|: absolute values of individual weighted residuals. Figure G: Basic goodness of fit plots of intracellular dCTP model. Red line represents average values. Black line represents theoretical values. Data from the same individual are shown in blue circles and are connected by lines. |iWRES|: absolute values of individual weighted residuals. Figure H: Basic goodness of fit plots of intracellular dGTP model. Red line represents average values. Black line represents theoretical values. Data from the same individual are shown in blue circles and are connected by lines. |iWRES|: absolute values of individual weighted residuals. Figure I: Basic goodness of fit plots of intracellular TTP model. Red line represents average values. Black line represents theoretical values. Data from the same individual are shown in blue circles and are connected by lines. |iWRES|: absolute values of individual weighted residuals. Figure J: An example of the plasma TFV/FTC vs the intracellular TFV-DP/FTC-TP “handshape” plot. Arrows indicate the progression of time after treatment initiation. NONMEM Code.

https://doi.org/10.1371/journal.pone.0165505.s001

(PDF)

Acknowledgments

We would like to thank Gilead Sciences Inc., which provided the study drug. We would also like to thank all of the volunteers who participated in the study, as well as the study personnel and Colorado Clinical Translational Research Center staff.

Author Contributions

  1. Conceptualization: XC PLA JJK SM.
  2. Data curation: LRB XC SMS PLA.
  3. Formal analysis: XC SM SMS PLA.
  4. Funding acquisition: PLA SMS XC.
  5. Investigation: XC SMS JRCM LRB JHZ JJK SM PLA.
  6. Methodology: XC LRB JHZ XC SM PLA.
  7. Project administration: PLA LRB.
  8. Resources: PLA JJK.
  9. Supervision: SM LRB JJK PLA.
  10. Validation: XC SM PLA LRB.
  11. Visualization: XC SM PLA.
  12. Writing – original draft: XC PLA SM.
  13. Writing – review & editing: XC SMS JRCM LRB JHZ JJK SM PLA.

References

  1. 1. Chapman T, McGavin J, Noble S. Tenofovir disoproxil fumarate. Drugs. 2003;63(15):1597–608. Epub 2003/07/31. pmid:12887265.
  2. 2. Kearney BP, Flaherty JF, Shah J. Tenofovir disoproxil fumarate: clinical pharmacology and pharmacokinetics. Clinical pharmacokinetics. 2004;43(9):595–612. Epub 2004/06/26. pmid:15217303.
  3. 3. Naesens L, Balzarini J, Bischofberger N, De Clercq E. Antiretroviral activity and pharmacokinetics in mice of oral bis(pivaloyloxymethyl)-9-(2-phosphonylmethoxyethyl)adenine, the bis(pivaloyloxymethyl) ester prodrug of 9-(2-phosphonylmethoxyethyl)adenine. Antimicrob Agents Chemother. 1996;40(1):22–8. Epub 1996/01/01. pmid:8787873; PubMed Central PMCID: PMCPMC163050.
  4. 4. Varga A, Graczer E, Chaloin L, Liliom K, Zavodszky P, Lionne C, et al. Selectivity of kinases on the activation of tenofovir, an anti-HIV agent. Eur J Pharm Sci. 2013;48(1–2):307–15. pmid:23201309.
  5. 5. Madrasi K, Burns RN, Hendrix CW, Fossler MJ, Chaturvedula A. Linking the population pharmacokinetics of tenofovir and its metabolites with its cellular uptake and metabolism. CPT: pharmacometrics & systems pharmacology. 3. United States2014. p. e147.
  6. 6. Duwal S, Schutte C, von Kleist M. Pharmacokinetics and pharmacodynamics of the reverse transcriptase inhibitor tenofovir and prophylactic efficacy against HIV-1 infection. PLoS One. 2012;7(7):e40382. Epub 2012/07/19. pmid:22808148; PubMed Central PMCID: PMCPMC3394807.
  7. 7. Baheti G, Kiser JJ, Havens PL, Fletcher CV. Plasma and intracellular population pharmacokinetic analysis of tenofovir in HIV-1-infected patients. Antimicrob Agents Chemother. 2011;55(11):5294–9. pmid:21896913; PubMed Central PMCID: PMCPMC3194996.
  8. 8. Baheti G, King JR, Acosta EP, Fletcher CV. Age-related differences in plasma and intracellular tenofovir concentrations in HIV-1-infected children, adolescents and adults. AIDS. 2013;27(2):221–5. Epub 2012/10/04. pmid:23032419; PubMed Central PMCID: PMCPMC3586236.
  9. 9. Barditch-Crovo P, Deeks SG, Collier A, Safrin S, Coakley DF, Miller M, et al. Phase i/ii trial of the pharmacokinetics, safety, and antiretroviral activity of tenofovir disoproxil fumarate in human immunodeficiency virus-infected adults. Antimicrob Agents Chemother. 2001;45(10):2733–9. Epub 2001/09/15. pmid:11557462; PubMed Central PMCID: PMCPMC90724.
  10. 10. Droste JA, Verweij-van Wissen CP, Kearney BP, Buffels R, Vanhorssen PJ, Hekster YA, et al. Pharmacokinetic study of tenofovir disoproxil fumarate combined with rifampin in healthy volunteers. Antimicrob Agents Chemother. 2005;49(2):680–4. Epub 2005/01/28. pmid:15673751; PubMed Central PMCID: PMCPMC547290.
  11. 11. Hirt D, Urien S, Ekouevi DK, Rey E, Arrive E, Blanche S, et al. Population pharmacokinetics of tenofovir in HIV-1-infected pregnant women and their neonates (ANRS 12109). Clin Pharmacol Ther. 2009;85(2):182–9. Epub 2008/11/07. pmid:18987623.
  12. 12. Jackson A, Moyle G, Watson V, Tjia J, Ammara A, Back D, et al. Tenofovir, emtricitabine intracellular and plasma, and efavirenz plasma concentration decay following drug intake cessation: implications for HIV treatment and prevention. J Acquir Immune Defic Syndr. 2013;62(3):275–81. Epub 2013/01/01. pmid:23274933.
  13. 13. Uglietti A, Zanaboni D, Gnarini M, Maserati R. Emtricitabine/tenofovir in the treatment of HIV infection: current PK/PD evaluation. Expert Opin Drug Metab Toxicol. 2012;8(10):1305–14. Epub 2012/09/05. pmid:22943210.
  14. 14. Valade E, Treluyer JM, Bouazza N, Ghosn J, Foissac F, Benaboud S, et al. Population pharmacokinetics of emtricitabine in HIV-1-infected adult patients. Antimicrob Agents Chemother. 2014;58(4):2256–61. pmid:24492366; PubMed Central PMCID: PMCPMC4023733.
  15. 15. Valade E, Treluyer JM, Illamola SM, Bouazza N, Foissac F, De Sousa Mendes M, et al. Emtricitabine seminal plasma and blood plasma population pharmacokinetics in HIV-infected men in the EVARIST ANRS-EP 49 study. Antimicrob Agents Chemother. 2015;59(11):6800–6. Epub 2015/08/19. pmid:26282407; PubMed Central PMCID: PMCPMC4604421.
  16. 16. Anderson PL, Glidden DV, Liu A, Buchbinder S, Lama JR, Guanira JV, et al. Emtricitabine-tenofovir concentrations and pre-exposure prophylaxis efficacy in men who have sex with men. Sci Transl Med. 2012;4(151):151ra25. pmid:22972843; PubMed Central PMCID: PMCPMC3721979.
  17. 17. Cressey TR, Avihingsanon A, Halue G, Leenasirimakul P, Sukrakanchana PO, Tawon Y, et al. Plasma and Intracellular Pharmacokinetics of Tenofovir Disoproxil Fumarate 300 mg Every 48 Hours vs 150 mg Once Daily in HIV-Infected Adults With Moderate Renal Function Impairment. Clin Infect Dis. 2015;61(4):633–9. Epub 2015/04/30. pmid:25921689; PubMed Central PMCID: PMCPMC4607735.
  18. 18. Dickinson L, Yapa HM, Jackson A, Moyle G, Else L, Amara A, et al. Plasma Tenofovir, Emtricitabine, and Rilpivirine and Intracellular Tenofovir Diphosphate and Emtricitabine Triphosphate Pharmacokinetics following Drug Intake Cessation. Antimicrob Agents Chemother. 2015;59(10):6080–6. Epub 2015/07/22. pmid:26195515; PubMed Central PMCID: PMCPMC4576083.
  19. 19. Goicoechea M, Jain S, Bi L, Kemper C, Daar ES, Diamond C, et al. Abacavir and tenofovir disoproxil fumarate co-administration results in a nonadditive antiviral effect in HIV-1-infected patients. AIDS. 2010;24(5):707–16. pmid:20087154.
  20. 20. Hawkins T, Veikley W, Durand-Gasselin L, Babusis D, Reddy YS, Flaherty JF, et al. Intracellular nucleotide levels during coadministration of tenofovir disoproxil fumarate and didanosine in HIV-1-infected patients. Antimicrob Agents Chemother. 2011;55(4):1549–55. Epub 2011/02/02. pmid:21282432; PubMed Central PMCID: PMCPMC3067129.
  21. 21. Hawkins T, Veikley W, St Claire RL 3rd, Guyer B, Clark N, Kearney BP. Intracellular pharmacokinetics of tenofovir diphosphate, carbovir triphosphate, and lamivudine triphosphate in patients receiving triple-nucleoside regimens. J Acquir Immune Defic Syndr. 2005;39(4):406–11. Epub 2005/07/13. pmid:16010161.
  22. 22. Seifert SM, Chen X, Meditz AL, Castillo-Mancilla JR, Gardner EM, Predhomme JA, Clayton C, Austin G, Palmer BE, Zheng JH, Klein B, Kerr BJ, Guida LA, Rower C, Rower JE, Kiser JJ, Bushman LR, MaWhinney S, Anderson PL. Intracellular Tenofovir and Emtricitabine Anabolites in Genital, Rectal, and Blood Compartments from First Dose to Steady State. AIDS Res Hum Retroviruses. 2016 Sep 19. [Epub ahead of print] 27526873
  23. 23. von Kleist M, Huisinga W. Pharmacokinetic-pharmacodynamic relationship of NRTIs and its connection to viral escape: an example based on zidovudine. Eur J Pharm Sci. 2009;36(4–5):532–43. Epub 2009/01/20. pmid:19150497.
  24. 24. Burns RN, Hendrix CW, Chaturvedula A. Population pharmacokinetics of tenofovir and tenofovir-diphosphate in healthy women. J Clin Pharmacol. 2015;55(6):629–38. Epub 01/13. pmid:25581815.
  25. 25. Jullien V, Treluyer JM, Rey E, Jaffray P, Krivine A, Moachon L, et al. Population pharmacokinetics of tenofovir in human immunodeficiency virus-infected patients taking highly active antiretroviral therapy. Antimicrob Agents Chemother. 2005;49(8):3361–6. Epub 2005/07/29. pmid:16048948; PubMed Central PMCID: PMCPMC1196246.
  26. 26. Biswas N, Rodriguez-Garcia M, Crist SG, Shen Z, Bodwell JE, Fahey JV, et al. Effect of tenofovir on nucleotidases and cytokines in HIV-1 target cells. PLoS One. 2013;8(10):e78814. Epub 2013/11/10. pmid:24205323; PubMed Central PMCID: PMCPMC3808279.
  27. 27. Vela JE, Miller MD, Rhodes GR, Ray AS. Effect of nucleoside and nucleotide reverse transcriptase inhibitors of HIV on endogenous nucleotide pools. Antivir Ther. 2008;13(6):789–97. Epub 2008/10/09. pmid:18839780.
  28. 28. Ray AS, Olson L, Fridland A. Role of purine nucleoside phosphorylase in interactions between 2',3'-dideoxyinosine and allopurinol, ganciclovir, or tenofovir. Antimicrob Agents Chemother. 2004;48(4):1089–95. Epub 2004/03/30. pmid:15047506; PubMed Central PMCID: PMCPMC375307.
  29. 29. Bantia S, Ananth SL, Parker CD, Horn LL, Upshaw R. Mechanism of inhibition of T-acute lymphoblastic leukemia cells by PNP inhibitor—BCX-1777. International immunopharmacology. 2003;3(6):879–87. Epub 2003/06/05. pmid:12781704.
  30. 30. Chen X, Castillo-Mancilla JR, Seifert SM, McAllister KB, Zheng JH, Bushman LR, et al. Analysis of the endogenous deoxynucleoside triphosphate pool in HIV positive and negative individuals receiving tenofovir-emtricitabine. Antimicrob Agents Chemother. 2016. Epub 2016/06/30. pmid:27353267.
  31. 31. Cottrell ML, Yang KH, Prince HM, Sykes C, White N, Malone S, et al. A Translational Pharmacology Approach to Predicting Outcomes of Preexposure Prophylaxis Against HIV in Men and Women Using Tenofovir Disoproxil Fumarate With or Without Emtricitabine. J Infect Dis. 2016. pmid:26917574.
  32. 32. Sahin S, Benet LZ. The operational multiple dosing half-life: a key to defining drug accumulation in patients and to designing extended release dosage forms. Pharm Res. 2008;25(12):2869–77. Epub 2008/11/19. pmid:19015955; PubMed Central PMCID: PMCPMC3581066.
  33. 33. Molina JM, Capitant C, Spire B, Pialoux G, Cotte L, Charreau I, et al. On-Demand Preexposure Prophylaxis in Men at High Risk for HIV-1 Infection. N Engl J Med. 2015;373(23):2237–46. Epub 2015/12/02. pmid:26624850.
  34. 34. Bushman LR, Kiser JJ, Rower JE, Klein B, Zheng JH, Ray ML, et al. Determination of nucleoside analog mono-, di-, and tri-phosphates in cellular matrix by solid phase extraction and ultra-sensitive LC-MS/MS detection. J Pharm Biomed Anal. 2011;56(2):390–401. Epub 2011/07/01. pmid:21715120; PubMed Central PMCID: PMCPMC3153375.
  35. 35. Delahunty T, Bushman L, Robbins B, Fletcher CV. The simultaneous assay of tenofovir and emtricitabine in plasma using LC/MS/MS and isotopically labeled internal standards. J Chromatogr B Analyt Technol Biomed Life Sci. 2009;877(20–21):1907–14. Epub 2009/06/06. pmid:19493710; PubMed Central PMCID: PMCPmc2714254.
  36. 36. Chen X, McAllister KJ, Klein B, Bushman LR, Anderson PL. Development and validation of an LC-MS/MS quantitative method for endogenous deoxynucleoside triphosphates in cellular lysate. Biomed Chromatogr. 2016 Aug 24. pmid:27557296.
  37. 37. Ahn JE, Karlsson MO, Dunne A, Ludden TM. Likelihood based approaches to handling data below the quantification limit using NONMEM VI. J Pharmacokinet Pharmacodyn. 2008;35(4):401–21. pmid:18686017.
  38. 38. Beal SL. Ways to fit a PK model with some data below the quantification limit. J Pharmacokinet Pharmacodyn. 2001;28(5):481–504. pmid:11768292.
  39. 39. Zhang L, Beal SL, Sheiner LB. Simultaneous vs. sequential analysis for population PK/PD data I: best-case performance. J Pharmacokinet Pharmacodyn. 2003;30(6):387–404. pmid:15000421.
  40. 40. Zhang L, Beal SL, Sheinerz LB. Simultaneous vs. sequential analysis for population PK/PD data II: robustness of methods. J Pharmacokinet Pharmacodyn. 2003;30(6):405–16. pmid:15000422.
  41. 41. Porchet HC, Benowitz NL, Sheiner LB. Pharmacodynamic model of tolerance: application to nicotine. J Pharmacol Exp Ther. 1988;244(1):231–6. pmid:3336000.
  42. 42. Jusko WJ, Ko HC, Ebling WF. Convergence of direct and indirect pharmacodynamic response models. J Pharmacokinet Biopharm. 1995;23(1):5–8; discussion 9–10. pmid:8576844.
  43. 43. Sharma A, Jusko WJ. Characteristics of indirect pharmacodynamic models and applications to clinical drug responses. Br J Clin Pharmacol. 1998;45(3):229–39. pmid:9517366; PubMed Central PMCID: PMCPMC1873365.
  44. 44. Sun YN, Jusko WJ. Role of baseline parameters in determining indirect pharmacodynamic responses. J Pharm Sci. 1999;88(10):987–90. pmid:10514344.
  45. 45. Dansirikul C, Silber HE, Karlsson MO. Approaches to handling pharmacodynamic baseline responses. J Pharmacokinet Pharmacodyn. 2008;35(3):269–83. pmid:18446428.
  46. 46. Hutmacher MM, Kowalski KG. Covariate selection in pharmacometric analyses: a review of methods. Br J Clin Pharmacol. 2015;79(1):132–47. pmid:24962797; PubMed Central PMCID: PMCPMC4294083.
  47. 47. Jonsson EN, Karlsson MO. Xpose—an S-PLUS based population pharmacokinetic/pharmacodynamic model building aid for NONMEM. Computer methods and programs in biomedicine. 1999;58(1):51–64. Epub 1999/04/09. pmid:10195646.
  48. 48. Borroto-Esoda K, Vela JE, Myrick F, Ray AS, Miller MD. In vitro evaluation of the anti-HIV activity and metabolic interactions of tenofovir and emtricitabine. Antivir Ther. 2006;11(3):377–84. pmid:16759055.
  49. 49. Stein DS, Moore KH. Phosphorylation of nucleoside analog antiretrovirals: a review for clinicians. Pharmacotherapy. 2001;21(1):11–34. Epub 2001/02/24. pmid:11191730.
  50. 50. Anderson PL, Kiser JJ, Gardner EM, Rower JE, Meditz A, Grant RM. Pharmacological considerations for tenofovir and emtricitabine to prevent HIV infection. J Antimicrob Chemother. 2011;66(2):240–50. pmid:21118913; PubMed Central PMCID: PMCPMC3019086.
  51. 51. Bourdais J, Biondi R, Sarfati S, Guerreiro C, Lascu I, Janin J, et al. Cellular phosphorylation of anti-HIV nucleosides. Role of nucleoside diphosphate kinase. J Biol Chem. 1996;271(14):7887–90. Epub 1996/04/05. pmid:8626464.
  52. 52. Skladanowski AC. The role of soluble 5'-nucleotidases in the conversion of nucleotide analogs: metabolic and therapeutic aspects. Current medicinal chemistry. 2013;20(34):4249–59. Epub 2013/09/03. pmid:23992311.
  53. 53. Rower JE, Meditz A, Gardner EM, Lichtenstein K, Predhomme J, Bushman LR, et al. Effect of HIV-1 infection and sex on the cellular pharmacology of the antiretroviral drugs zidovudine and lamivudine. Antimicrob Agents Chemother. 2012;56(6):3011–9. pmid:22391541; PubMed Central PMCID: PMCPMC3370731.
  54. 54. Molina JM, Peytavin G, Perusat S, Lascoux-Combes C, Sereni D, Rozenbaum W, et al. Pharmacokinetics of emtricitabine, didanosine and efavirenz administered once-daily for the treatment of HIV-infected adults (pharmacokinetic substudy of the ANRS 091 trial). HIV medicine. 2004;5(2):99–104. pmid:15012649.
  55. 55. Deeks ED, Perry CM. Efavirenz/emtricitabine/tenofovir disoproxil fumarate single-tablet regimen (Atripla(R)): a review of its use in the management of HIV infection. Drugs. 2010;70(17):2315–38. Epub 2010/11/18. pmid:21080746.
  56. 56. Ramanathan S, Shen G, Cheng A, Kearney BP. Pharmacokinetics of emtricitabine, tenofovir, and GS-9137 following coadministration of emtricitabine/tenofovir disoproxil fumarate and ritonavir-boosted GS-9137. J Acquir Immune Defic Syndr. 2007;45(3):274–9. Epub 2007/04/07. pmid:17414929.
  57. 57. Chittick GE, Zong J, Blum MR, Sorbel JJ, Begley JA, Adda N, et al. Pharmacokinetics of tenofovir disoproxil fumarate and ritonavir-boosted saquinavir mesylate administered alone or in combination at steady state. Antimicrob Agents Chemother. 2006;50(4):1304–10. Epub 2006/03/30. pmid:16569845; PubMed Central PMCID: PMCPMC1426992.
  58. 58. Blum MR, Chittick GE, Begley JA, Zong J. Steady-state pharmacokinetics of emtricitabine and tenofovir disoproxil fumarate administered alone and in combination in healthy volunteers. J Clin Pharmacol. 2007;47(6):751–9. Epub 2007/05/24. pmid:17519400.
  59. 59. Wang LH, Begley J, St Claire RL 3rd, Harris J, Wakeford C, Rousseau FS. Pharmacokinetic and pharmacodynamic characteristics of emtricitabine support its once daily dosing for the treatment of HIV infection. AIDS Res Hum Retroviruses. 2004;20(11):1173–82. Epub 2004/12/14. pmid:15588339.
  60. 60. Castillo-Mancilla JR, Zheng JH, Rower JE, Meditz A, Gardner EM, Predhomme J, et al. Tenofovir, emtricitabine, and tenofovir diphosphate in dried blood spots for determining recent and cumulative drug exposure. AIDS Res Hum Retroviruses. 2013;29(2):384–90. Epub 2012/09/01. pmid:22935078; PubMed Central PMCID: PMCPMC3552442.
  61. 61. Deeks SG, Barditch-Crovo P, Lietman PS, Hwang F, Cundy KC, Rooney JF, et al. Safety, pharmacokinetics, and antiretroviral activity of intravenous 9-[2-(R)-(Phosphonomethoxy)propyl]adenine, a novel anti-human immunodeficiency virus (HIV) therapy, in HIV-infected adults. Antimicrob Agents Chemother. 1998;42(9):2380–4. Epub 1998/09/16. pmid:9736567; PubMed Central PMCID: PMCPMC105837.
  62. 62. Pruvost A, Negredo E, Benech H, Theodoro F, Puig J, Grau E, et al. Measurement of intracellular didanosine and tenofovir phosphorylated metabolites and possible interaction of the two drugs in human immunodeficiency virus-infected patients. Antimicrob Agents Chemother. 2005;49(5):1907–14. pmid:15855513; PubMed Central PMCID: PMCPMC1087635.
  63. 63. Patterson KB, Prince HA, Kraft E, Jenkins AJ, Shaheen NJ, Rooney JF, et al. Penetration of tenofovir and emtricitabine in mucosal tissues: implications for prevention of HIV-1 transmission. Sci Transl Med. 2011;3(112):112re4. Epub 2011/12/14. pmid:22158861; PubMed Central PMCID: PMCPMC3483088.