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Correction: Sirtuin-2 Regulates Sepsis Inflammation in ob/ob Mice

  • Xianfeng Wang,
  • Nancy L. Buechler,
  • Ayana Martin,
  • Jonathan Wells,
  • Barbara Yoza,
  • Charles E. McCall,
  • Vidula Vachharajani

Correction: Sirtuin-2 Regulates Sepsis Inflammation in ob/ob Mice

  • Xianfeng Wang, 
  • Nancy L. Buechler, 
  • Ayana Martin, 
  • Jonathan Wells, 
  • Barbara Yoza, 
  • Charles E. McCall, 
  • Vidula Vachharajani
PLOS
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Bar graphs are missing from Fig 3A of the published article. Please see the correct Fig 3 here.

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Fig 3. Role of SIRT-2 in the endotoxin tolerant RAW cells.

A: RAW cells undergo endotoxin tolerance as early as 4h after LPS stimulation: To study endotoxin tolerance in RAW cells, we stimulated cells with LPS (100ng/ml) and re-stimulated with another LPS challenge (100ng/ml) at 0, 4, 6, 8, and 24 hour time points for four hour. We studied TNF-α mRNA expression. The cells increased TNF-α mRNA expression in response to LPS re-stimulation only at 0h time point. RAW cells were unable to increase TNF-α mRNA further to LPS re-stimulation at 4, 6, 8 and 24h time points, indicating endotoxin tolerance. * p<0.05 vs. respective NS group Tukey‘s post-hoc analysis; error bars: s.e.m. B: SIRT-2 protein expression increased during endotoxin tolerant phase in RAW cells: RAW cells were treated with LPS (100ng/ml) for 0, 1, 4, 8, 18, and 24 h. Whole cell lysates were collected for western blotting of proteins SIRT-2 and CPA (housekeeping gene). Representative image out of three experiments shows that was increased in SIRT-2 expression in 18 and 24h after LPS stimulation. C SIRT-2 deacetylates NFkB p65: We studied the effect of SIRT-2 expression on NFkB p65 acetylation using HEK293 cells. SIRT-2 plasmid was co-transfected with p65 or/and CBP plasmids into HEK293 cells (to increase baseline p65 acetylation) and blotted for antibodies against Ac-p65, total p65, SIRT-2 and GAPDH. NFkB p65 acetylation (Ac-p65) increased in cells with transfection with p65+CBP while it decreased in cells transfected with p65+CBP+SIRT-2, indicating SIRT-2 directly deacetylates NFkB p65.

https://doi.org/10.1371/journal.pone.0162560.g001

Reference

  1. 1. Wang X, Buechler NL, Martin A, Wells J, Yoza B, McCall CE, et al. (2016) Sirtuin-2 Regulates Sepsis Inflammation in ob/ob Mice. PLoS ONE 11(8): e0160431. pmid:27500833