S1 Fig. Ectopic expression of cellulase in E. coli.
Bacteria were cultured in LB medium at 37°C to OD600 0.5. The recombinant proteins were induced for 3 h by supplementation with 0.5 mM IPTG. Cells were harvested, washed in PBS, and then resuspended in 10 mM PBS (pH7.5, 500 mM NaCl). After several freeze/thaw cycles, the cell suspension was sonicated for 3 min with an interval of 4 s between pulses, and then centrifuged at 5000 g for 10 min at 37°C. Twenty microliter supernatant samples were analysed by 12% SDS-PAGE.
S1 Table. Strains and plasmids used in this study.
Citation: Xia T, Li Y, Sun D, Zhuo T, Fan X, Zou H (2016) Correction: Identification of an Extracellular Endoglucanase That Is Required for Full Virulence in Xanthomonas citri subsp. citri. PLoS ONE 11(5): e0156458. https://doi.org/10.1371/journal.pone.0156458
Published: May 23, 2016
Copyright: © 2016 Xia et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.