Awake craniotomy (AC) renders an expanded role in functional neurosurgery. Yet, evidence for optimal anaesthesia management remains limited. We aimed to summarise the latest clinical evidence of AC anaesthesia management and explore the relationship of AC failures on the used anaesthesia techniques.
Two authors performed independently a systematic search of English articles in PubMed and EMBASE database 1/2007-12/2015. Search included randomised controlled trials (RCTs), observational trials, and case reports (n>4 cases), which reported anaesthetic approach for AC and at least one of our pre-specified outcomes: intraoperative seizures, hypoxia, arterial hypertension, nausea and vomiting, neurological dysfunction, conversion into general anaesthesia and failure of AC. Random effects meta-analysis was used to estimate event rates for four outcomes. Relationship with anaesthesia technique was explored using logistic meta-regression, calculating the odds ratios (OR) and 95% confidence intervals [95%CI].
We have included forty-seven studies. Eighteen reported asleep-awake-asleep technique (SAS), twenty-seven monitored anaesthesia care (MAC), one reported both and one used the awake-awake-awake technique (AAA). Proportions of AC failures, intraoperative seizures, new neurological dysfunction and conversion into general anaesthesia (GA) were 2% [95%CI:1–3], 8% [95%CI:6–11], 17% [95%CI:12–23] and 2% [95%CI:2–3], respectively. Meta-regression of SAS and MAC technique did not reveal any relevant differences between outcomes explained by the technique, except for conversion into GA. Estimated OR comparing SAS to MAC for AC failures was 0.98 [95%CI:0.36–2.69], 1.01 [95%CI:0.52–1.88] for seizures, 1.66 [95%CI:1.35–3.70] for new neurological dysfunction and 2.17 [95%CI:1.22–3.85] for conversion into GA. The latter result has to be interpreted cautiously. It is based on one retrospective high-risk of bias study and significance was abolished in a sensitivity analysis of only prospectively conducted studies.
Citation: Stevanovic A, Rossaint R, Veldeman M, Bilotta F, Coburn M (2016) Anaesthesia Management for Awake Craniotomy: Systematic Review and Meta-Analysis. PLoS ONE11(5): e0156448. https://doi.org/10.1371/journal.pone.0156448
Editor: Marco Gemma, Scientific Inst. S. Raffaele Hosp., ITALY
Received: December 25, 2015; Accepted: May 13, 2016; Published: May 26, 2016
Copyright: © 2016 Stevanovic et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All relevant data are within the paper and its Supporting Information files.
Funding: The authors received no specific funding for this work.
Competing interests: The authors have declared that no competing interests exist.
Awake craniotomy (AC) was initially used for removal of epileptic foci with simultaneous application of brain mapping and electrical current. Since the 1980s further developments brought this technique into use for resection of tumours involving functional cortex . AC with live intraoperative brain mapping and monitoring of neurological function and neurocognitive performance, allows maximal resection of malignant gliomas with a favourable survival prognosis and without language deficits . Tumour resection is adapted to the individual anatomy of the patient, which generally shows huge inter-individual variability [2,3]. The primary aim is to preserve or even improve the complex human brain function, while achieving maximal removal of tumours or epileptic foci . Given the effectiveness of AC for resection of eloquent tumours, data suggest an expanded role for AC in brain tumour surgery regardless of tumour location . In addition, ACs are established functional neurosurgical approaches for deep-brain stimulation within treatment of Parkinson´s disease and obsessive-compulsive disorders [6,7]. Recent data suggest that postoperative deficits are less frequent compared to general anaesthesia (GA) . Yet, there is an array of tasks, which have to be accomplished by the anaesthesiologist to avoid complications during ACs. Although anaesthesia for AC is usually well tolerated it requires an extensive knowledge of the principles underlying neuroanaesthesia and the special technical strategies including local anaesthesia for scalp blockade, advanced airway management, dedicated sedation protocols, and skilful management of haemodynamics . One systematic review performed in 2013, focused on the anaesthesia technique for craniotomy . They included only eight studies, published until 2012, which compared GA to AC, but the anaesthetic approach used for AC was not analysed in detail . Nowadays the mainly used anaesthetic techniques for AC include the asleep-awake-asleep (SAS) technique, monitored anaesthesia care (MAC), and the recent introduced awake-awake-awake (AAA) method. SAS is the oldest technique, using GA before and after brain mapping. MAC, also called "conscious sedation" is a mild form of sedation, where the patients`anxiety and pain are controlled, while the patients are able to follow orders and to protect their airways without invasive airway devices . AAA technique only consists of local or regional anaesthesia supplemented with intravenous analgesia but avoiding any sedative anaesthetic. Still, no consensus exists on the optimal anaesthesiological management for AC. In consequence, we decided to analyse the recent evidence of benefits and harms resulting from the different anaesthesia techniques for AC.
We aimed to add to existing knowledge about the process of anaesthesia care for AC, the benefits and harms of the three anaesthesia techniques (MAC, SAS and AAA) for adult patients, from clinical studies published between January 2007 and December 2015. The primary outcome of interest was the incidence of AC failures, related to the used anaesthesia technique. We reviewed the study-, patient-, anaesthesia- and intraoperative-characteristics, including adverse events and postoperative outcomes.
Materials and Methods
A protocol with the inclusion and exclusion criteria for suitable studies and the method of analysis were established with all authors. The protocol was not published. This systematic review was prepared in accordance with the PRISMA guidelines  (see S1 Checklist).
This systematic review (SR) was registered in the International Prospective Register of Systematic Reviews (PROSPERO; http://www.crd.york.ac.uk/PROSPERO, CRD42015025376).
Types of studies: Publication types suitable for inclusion were randomised controlled clinical trials (RCTs), prospective and retrospective observational clinical trials, and case reports with more than four clinical cases. We excluded animal studies, reviews, paediatric studies, studies on pregnant women, other topics, abstracts, letters, and Non-English publications.
Types of participants: The included studies had to report on patients undergoing AC for resection of epileptic foci and tumours that involve eloquent (motor, sensory and language) brain cortices. The studies should be performed in an operating room and describe the anaesthetic approach used for AC. Additionally, they had to report data for at least one of the following outcome variables: intraoperative seizures, hypoxia and arterial hypertension, intra-/ postoperative nausea and vomiting (PONV), new postoperative neurological dysfunction, conversion to GA and failure of AC. Of note, only studies reporting on adult patients (≥18 years of age) were initially considered. It became apparent, that some studies, even with the applied children excluding filter in our search strategy, also reported on AC procedures in several children. Despite this, the mean age in these studies corresponded to adults and after discussion with all authors we decided not to exclude these studies, as they were not real solely paediatric studies.
Types of intervention: We included studies, which reported on one of the following three anaesthetic approaches: Asleep-awake-asleep (SAS) technique, monitored anaesthesia care (MAC), and the awake-awake-awake (AAA) technique.
A PubMed and EMBASE database search was carried out for the time frame from 01.01.2007 until 31.12.2015. The search and screening process were independently carried out by MC and AS. Additionally the reference lists of the included articles were scanned for further eligible studies. One author was contacted, to provide additional study information .
EMBASE and PubMed search strategy are shown in S1 File. Records identified through PubMed and EMBASE were hand-searched on basis of the title and abstract. Resulting records were then hand-searched on basis of the full text and records not matching the topic of this SR were excluded. Of note, articles reporting studies conducted outside the operating room, like in a MRI suite, or with the use of an intraoperative MRI guidance were excluded.
Study selection and data collection.
MC and AS screened the titles independently and removed articles that did not meet the pre-specified screening criteria, or were duplicate articles. The remaining articles were screened on the basis of their abstract. All apparently eligible articles were analysed in detail, according to a pre-piloted form, by their full text. Any uncertainties were discussed between the two primary review authors. In the event of persistent disagreement, all other authors were integrated in the discussion until consensus was achieved. Articles were also double checked for not apparently study-duplicates, in regard to juxtaposed author names, treatment comparisons, sample sizes, and outcomes. It was planned to contact the authors, if important outcome parameter are missing, and the study met our inclusion criteria.
AS and MC extracted the following data from each included study: 1.) Study characteristics (study design, recruitment period, sample size, comparative group, endpoint/ aim of the study, study conclusion). 2.) Anaesthesia characteristics (kind of technique, drugs and dosages, patient airway). 3.) Patient characteristics (gender, age and kind of tumour). 4.) Intraoperative characteristics and adverse events (surgery durations, AC failures, conversion to GA, hypoxia, arterial hypertension and seizures). An AC failure was not only considered if conversion to GA was required, but also if adequate awake brain mapping/ monitoring could not have been achieved due to the patient condition e.g. seizures, dysphasia, somnolence, agitation or physical complications. 5.) Patient outcomes (including neurological dysfunctions, mortality, postoperative intracranial haematoma, amount of total tumour resection and the length of hospital stay). Our initial protocol sought to precise the postoperative neurological outcomes into subtypes like hemiplegia, hemiparesis, verbal dysfunctions etc., but the systematic search yielded a high diversity in the reported subtypes. Therefore, we decided with all authors to make a simplification into "new neurological dysfunction". This term included all kinds of neurological dysfunctions, but excluded deterioration of pre-existing neurological dysfunctions. RR, FB and MV checked independently the extracted data.
Risk of bias in individual studies.
For randomised controlled trials we used the Cochrane Collaboration’s risk of bias tool . For observational trials and case reports we used the Agency for Healthcare Research and Quality (AHRQ) tool . Risk of bias was assessed by MC and AS independently during the data extraction process and revealed an adequate reliability.
Summary measures and synthesis of results.
Our aim was to analyse multiple outcomes of AC patients, depending on the used anaesthesia technique. Our primary outcome of interest was the incidence of AC failure associated with the used anaesthesia techniques. The secondary outcomes included the complication rates, probably related to the used anaesthesia technique.
Pooled estimates of outcome measures with subgroup analyses depending on the anaesthetic approach were calculated if enough studies reported an outcome variable for the respective anaesthesia technique. This referred to the outcome variables AC failure, intraoperative seizure, conversion into GA and new neurological dysfunction. The DerSimonian-Laird random effects model using logit-transformed event proportions was applied, as we assumed a high within study and inter-study variation. The inter-study variation attributed to other reasons than chance was quantified by I2. The relationship of anaesthesia technique (MAC/ SAS) as one potential source of heterogeneity and the four above-described outcome measures (AC failure, intraoperative seizure, conversion to GA and new neurological dysfunction) was explored using logistic meta-regression with fixed effect for anaesthesia technique . Odds ratio (OR) and 95% confidence intervals [95%CIs] were determined and considered statistically significant when the 95%CI excluded 1. If studies included a high proportion of the same study-population, we considered only the largest study for the meta-analysis [14,15]. Analyses were performed using "R" version 3.0.2 ; for meta-analysis the meta package was used.
Risk of bias across studies.
Publication bias was not assessed in this systematic review. Selective reporting bias was assessed with the above-mentioned risk of bias tools.
Additional analyses were not pre-specified, but performed according to the request of the reviewers. Meta-analysis and meta-regression were performed for one composite outcome, comprising the life-threatening events AC failure, mortality and intraoperative seizures. Furthermore, a sensitivity analysis, by looking only at prospective studies, was conducted for the five outcomes, which were included in the meta-analyses. The relationship of anaesthesia technique (MAC/ SAS) as one potential source of heterogeneity and the five above-described outcomes (AC failure, intraoperative seizure, conversion to GA, new neurological dysfunction and the composite outcome) of prospective studies was explored using logistic meta-regression.
Our search strategy in EMBASE and PubMed initially revealed 1303 publications. We did not identify any additional studies by screening the reference lists. The detailed screening, eligibility assessment and inclusion process is shown in Fig 1. We included a total of forty-seven studies [10,17–62] in our SR. One author was personally contacted, and provided us more information about their used anaesthesia technique .
Data of the study characteristics are shown in Table 1. A total of fourteen case series [10,17,19,20,23,28,39,41,44,47,51,53,54,60] thirteen prospective studies [18,21,22,25–27,30,33,35,38,52,55,61], seventeen retrospective studies [24,29,31,34,37,40,42,43,45,46,48–50,57–59,62], two RCTs [32,56], and one pseudo-RCT  comprising 5945 AC procedures in 5931 patients were analysed (Table 1). Of note, during the data extraction process it appeared that nine studies [20,22,27,31,42–46] partially reported on the same patient population. This refers to the study of Grossman et al.  and both studies of Nossek et al. [42,43], two publications of Ouyang et al. [45,46] the publications of Boetto and Deras et al. [22,27] and at least the studies of Andersen and Olsen et al. [20,44]. After complete data extraction we discussed with all authors how to deal with these partial duplicates. Consensus was found to retain all publications for the study descriptions, as they have all reported some different outcomes in these patients, which could provide additional useful information and the patient population was not absolutely the same . In contrast, for a reasonable meta-analysis only the largest study of the duplicate studies was chosen, as the complete elimination of duplicate studies would bias the meta-analysis in its entirety [14,15].
Anaesthesia characteristics, including the kind of anaesthesia technique, used drugs and dosages and the description of the patient’s airway are presented in Tables 2 and 3. The patient characteristics are summarised in the S1 Table. Intraoperative characteristics and adverse events are shown in Table 4 and the patient outcomes in Table 5.
Risk of bias within and across studies.
The risk of bias was assessed with the Cochrane Collaboration’s risk of bias tool (S2 Table) for the RCTs and for the remaining studies with the Agency of Healthcare Research and Quality (AHRQ-tool)  (S3 Table). Both RCTs [36,56] and the pseudo-RCT  showed a high risk of selection and performance bias. Observational studies showed a high risk of detection bias and confounding bias. Furthermore, they showed a varied degree of other risks of biases inherent to the study design.
Results of individual studies.
We divided the identified records into three subtopics according to the used anaesthetic technique: Nineteen studies reported the asleep-awake-asleep (SAS) respectively sleep-awake (SA) technique [20–23,25–27,37–39,44–46,50,51,53,56,57,60], twenty-eight reported monitored anaesthesia care (MAC) [10,17–19,24,28–32,34–36,40–43,47–49,52,54,55,58–62] and one used the awake-awake-awake (AAA) technique . Of note, Souter et al. have used the SAS as well as the MAC technique in their patients .
Synthesis of results
General considerations for AC are provided in the S2 File.
The protocols of the nineteen identified articles [20–23,25–27,37–39,44–46,50,51,53,56,57,60], reporting the SA(S) technique, showed a huge variability in the anaesthesia conduction, but all kinds of this technique were feasible and safe for the patients. A total intravenous anaesthesia (TIVA) with propofol and remifentanil or fentanyl for the first asleep phase was used in fourteen trials [20–23,26,27,37,39,44–46,51,56,60]. Two studies used only propofol as sedative [25,38]. Dexmedetomidine, an alpha-2 adrenoceptor agonist, enables sedation, anxiolytic effects, and analgesia. It was successfully used for AC since 2001 . Dexmedetomidine was applied in four studies, either combined with remifentanil [34,56], or propofol , or with remifentanil and propofol together [53,57]. The use of dexmedetomidine seems to show several advantages in AC. Shen et al. compared the effect of dexmedetomidine- to propofol-based SAS technique . They showed that patients in the dexmedetomidine group had a shorter arousal time after the first asleep phase and a higher degree of surgeon satisfaction. Fast and sufficient recovery after craniotomy is a crucial factor for successful awake cortical mapping within adequate surgery time. A further study, showed reduction of pain induced haemodynamic reactions to pinning and incision, when AC with propofol, dexmedetomidine and local anaesthesia was performed (n = 101), compared to balanced GA (n = 77) . This could partly be explained by the analgesic and sympathic blockage effect of dexmedetomidine. Furthermore, the patients needed less intraoperative vasopressors and opioids compared to the GA group. Also postoperative requirement of opioids and antiemetic drugs was reduced in the AC group. Of note, in contrast to the AC group, a RSNB was not performed in all GA patients, which maybe accompanied by more opioid application and consecutive nausea. Conversely they observed more oxygen desaturations (SaO2 <90%) in the AC group, despite the absence of respiratory suppression by dexmedetomidine. This might be explained by the propofol saving effect of dexmedetomidine, which bears the risk of over sedation with propofol, especially during the painful beginning of the surgery. Of note, only one AC patient required the placement of a LMA. In contrast two GA patients showed significant postoperative desaturations and one of them required a re-intubation.
The airway in the included studies was secured either with a laryngeal mask (LMA) [21,25,26,38,45,46], an endotracheal tube in all ,respectively one patient [20,44] or an oesophageal naso-pharyngeal catheter . One study, which used a TIVA, did not mention the utilized airway device, they only reported naso-pharyngeal airway  and another one reported only an "oral airway" for five patients . Twelve studies [21,23,26,56] used controlled ventilation, the others maintained spontaneous breathing. A nasal cannula with spontaneous breathing was used in one trial [34,50] and Shinoura et al. did not report the ventilation mode .
Once the dura was opened and brain exposed, propofol was terminated and remifentanil and dexmedetomidine infusions were reduced or also stopped to allow patient awakening and removal of the airway device. In the study, which used the naso-pharyngeal catheter, the proximal balloon sealing the naso- and oro-pharyngeal cavities was deflated to allow patient vocalisation . Dexmedetomidine was also successfully used after cessation of propofol and fentanyl, during the awake resection phase of the SAS technique . Of note, this study reported the SAS technique for only two patients and concurrently the MAC technique for four patients. The second asleep phase was not described in detail in all included studies, but it consisted of sedative anaesthesia, remaining spontaneous breathing during wound closure up to controlled ventilation with endotracheal intubation like in the study of Deras et al. . Only four studies used a sleep-awake (SA) protocol for some patients  respective all patients [20,26,44]. Almost all patients undergoing SA(S) management underwent successful AC and the failure rate was minimal with 13 out of 1313 procedures (where failure rate was reported, and excluding the duplicate studies [27,44]). The meta-analysis showed a proportion of 2% [95%CI: 1–4] (Fig 2).
The summary value is an overall estimate from a random-effect model. The vertical dotted line shows an overall estimate of outcome proportion (based on the meta-analysis) disregarding grouping by technique. Of note, Souter et al.  have used both anaesthesia techniques.
MAC—monitored anaesthesia care.
Defined by the "American Society of Anesthesiologists" (ASA) this technique enables purposeful patient response to tactile or verbal stimulation, while preserving spontaneous ventilation without any airway instrumentation . Twenty-eight included studies reported the successful use of MAC in AC [10,17–19,24,28–32,34–36,40–43,47–49,52,54,55,58–62]. Except of three studies, which only used local infiltration anaesthesia [36,48,61] respectively one, which did not mention the use of infiltration anaesthesia , all others applied an additional RSNB at the beginning of the surgery. The airway was secured with an oxygen mask, a nasal cannula or an additional nasal trumpet under maintained spontaneous breathing. Except in two studies [17,61], the used anaesthetics consisted of all possible combinations of fentanyl, remifentanil, propofol, midazolam and dexmedetomidine. Abdou et al. applied in the same syringe a mixture of ketamine and propofol 1:1 ‘‘ketofol” , and Wrede et al. used piritramide and midazolam for their conscious sedation . Propofol and remifentanil were mostly discontinued 15 minutes before brain mapping and the patients did not receive any sedation and analgesia during the "awake" phase in thirteen studies [10,17,24,28–31,36,41–43,49,54], respectively three studies which applied only opioids if needed [34,47,52]. Three studies continued conscious sedation with propofol in a reduced dosage also during the awake phase [18,32], and the awake anaesthesia management is unknown for six studies [19,35,55,58,61,62]. Dexmedetomidine (around 0.1–0.7 μg kg-1 h-1) was continued in totally 36 procedures during the "awake" phase [48,59,60]. It could be shown, that dexmedetomidine has a minimal interference with electrocorticography (ECoG) during AC in a dosage of 0.2–0.5 μg kg-1 h-1 . Anaesthesia for the end of surgery was not described in detail in the identified MAC studies, but it may be assumed that the initial regime was resumed until skin closure. Interestingly, Peruzzi et al. used additional sevoflurane until the opening of dura mater, to decrease the amount of propofol .
Grossman et al. included in their study 90 elderly patients, with a mean age of 71.7±5.1 years, of totally 424 patients . Preservation of the neurological status has a strong impact on the quality of life in especially this population. They showed that a maximum gross total resection (GTR) of high-grade glioma under AC is feasible, without increased mortality or postoperative morbidity (including postoperative neurological complications) in elderly patients, compared to the younger one . Furthermore, their survival increases significantly compared to restrictive treatment like subtotal resections and biopsies. MAC for AC was also used efficaciously in five elderly patients (>60 years), with complex co-morbidities . Intraoperative hypoxia was reported for five patients [36,59], but all cases could be resolved with simple dose reduction and oxygen application. One large retrospective study (n = 611) used all possible combinations of propofol, remifentanil and dexmedetomidine in patients with significantly different baseline characteristics . Only high-risk patients (high body-mass-index (BMI), high tumour mass, high blood loss estimated) (n = 8) received a LMA for the initial procedure . The total rate of AC failure in all studies using the MAC technique and reporting the failure rate was 81 of totally 3616 procedures. Excluding the duplicate study of Nossek et al.  and Grossman et al.  which contained partially the same patients like the larger second study , our meta-analysis calculated with the random effects model revealed a proportion of a 2% failure rate [95%CI: 1–4] in 2700 procedures, which reported AC failure (Fig 2).
Hansen et al. were the first, who reported the awake-awake-awake technique avoiding sedatives in 47 patients undergoing 50 AC procedures by using RSNBs, permanent presence of a contact person, and therapeutic communication . Instead of using premedication with benzodiazepines, a strong pre-operative confidence with calming the patient was established during an extensive pre-operative personal visit of the attending anaesthesiologist. Subsequently the anaesthesiologist continuously guided the patients intraoperatively with strong rapport, physical contact and therapeutically communication. This included hypnotic positive suggestions like reframing disturbing surgery related noises and dissociation into a "safe place". Only two-thirds of the patients requested remifentanil with an average total dose of 156μg. Intraoperative vigilance tests showed equal or higher scores than preoperative tests. In the postoperative interview conducted in twenty-two patients, 73% of patients reported a lack of any discomfort, 95% felt “adequate prepared”, and 82% did not experience any fear at all. BIS monitoring was applied in all patients. The AC failure rate was minimal with one patient out of 50 AC procedures. This patient experienced general seizure, which could not be handled only with cold saline solution or minimal doses of propofol, but the surgery was smoothly continued in GA. A meta-analysis could not be performed for the AAA technique due to only one study reporting it.
A reasonable meta-analysis and logistic meta-regression could only be performed for four outcome variables: AC failures, seizures, conversion into general anaesthesia and new postoperative neurologic dysfunction based on the anaesthetic approach of MAC or SAS. The other variables were not reported frequently enough in the included studies for both kinds of anaesthesia technique. Mortality was reported in thirty-eight studies, but not included in the meta-analysis as a single outcome variable due to the extremely rare event rate. It was integrated in the composite outcome analysis together with AC failure and intraoperative seizure.
Our primary outcome of interest was the failure rate of AC, depending on the used anaesthesia technique. The meta-analysis for the proportion of awake craniotomy failures, depending on the used anaesthetic approach (MAC vs. SAS) included thirty-eight studies (Fig 2) [10,18–26,28,29,32,34–41,43,47–62]. It included the largest of the duplicate studies and excluded the smaller ones [27,42,44], which have also reported this outcome, according to Tramer et al.  and van Elm et al. . The particular reasons for AC failures are shown in Table 4 and included all cases where a complete intraoperative awake monitoring of the brain function during the tumour resection could not be achieved. Of note, an AC failure was not only restricted to the cases, where conversion to GA was required. The proportion of AC failures was 2% [95%CI 1–3], and the studies showed a substantial heterogeneity (I2 = 61%) (Fig 2). The relationship of the used technique (SAS/ MAC) as a possible source of the heterogeneity was explored using logistic meta-regression. The OR comparing SAS to MAC was 0.98 [CI95%: 0.36–2.69]. The employed anaesthesia technique did not explain a substantial portion of the heterogeneity between studies (QM = 0.001, df = 1, p = 0.972), and the test for residual heterogeneity was significant (QE = 93.70, df = 37, p < 0.001).
Conversion into general anaesthesia.
The discrepancy between the numbers of required conversion to GA and AC failure rates may be explained as follows: Not every AC failure required conversion into GA and not every conversion into GA was performed during the awake tumour resection phase, but also at the end of surgery, where it did not compromise the success of AC, like in the study of Sinha et al. . Forty-two studies reported 47 unplanned conversions into GA during totally 4971 AC procedures [10,17–29,31–37,39,40,42–44,47–62]. The particular reasons for unplanned conversion into GA are shown in Table 4. After exclusion of the duplicate studies [27,31,42,44] and the AAA study of Hansen et al. , our meta-analysis showed a total proportion of conversion into GA of 2% [95%CI 1–3] (Fig 3). Logistic meta-regression was also performed for this outcome, to analyse if the used technique (SAS/ MAC) may explain the differences between the studies. The OR comparing SAS to MAC was 2.17 [95%CI: 1.22–3.85] and the likelihood ratio test (LR test) showed a significant p-value of 0.03. However, the predicted proportion of conversions in the MAC and SAS group were not substantially different (MAC: 2% [95%CI: 1–2], SAS: 3% [95%CI: 2–5]).
The summary value is an overall estimate from a random-effect model. The vertical dotted line shows an overall estimate of outcome proportion (based on the meta-analysis) disregarding grouping by technique. Of note, Souter et al.  have used both anaesthesia techniques. GA, general anaesthesia.
Threatening adverse events during AC are seizures. The most seizures in the included studies were triggered by electrical cortical stimulation and were self-limited after cessation of cortical stimulation. The other could be treated with cold saline solution, or finally with anticonvulsive medication, or low doses of propofol, thiopental or benzodiazepines. Discontinuation of AC was rarely necessary. Thirty-nine studies reported the incidence of intraoperative seizures and their consequences [10,17–29,31–39,42–44,47,49–55,57–60,62]. The total number of performed AC procedures in these studies was 4942 and 351 (7.1%) intraoperative seizures were reported (Table 4). Only twenty-three (0.5%) intraoperative seizures led to a failure of AC, but they were resolved without any serious problems and the surgery was continued in GA [33,34,42,43,55,57]. Interestingly, the AAA technique showed a high proportion of eight seizures in fifty AC procedures, but only one led to AC failure due to required intubation .
Intraoperative seizures were more common in younger patients and those with a history of seizures [31,42]. A meta-analysis was performed for thirty-four studies, [10,17–26,28,29,32,34–39,43,47,49–55,57–60,62], which used the MAC and SAS technique, excluding the duplicate studies from Tel Aviv [31,42] and Glostrup [27,44]. Meta-analysis showed an estimated proportion of seizures of 8% [95%CI: 6–11] with substantial heterogeneity between studies (I2 = 75%) (Fig 4). In the meta-regression analysis, the techniques used did not explain the differences in the studies (QM < 0.001, df = 1, p = 0.983). The OR comparing SAS to MAC technique was 1.01 [CI95%: 0.52–1.88].
The summary value is an overall estimate from a random-effect model. The vertical dotted line shows an overall estimate of outcome proportion (based on the meta-analysis) disregarding grouping by technique. Of note, Souter et al.  have used both anaesthesia techniques.
Postoperative neurological dysfunction (new/ late).
Description of particular postoperative neurological dysfunctions differed significantly in the included studies. Therefore we have subsumed all kinds of new neurological dysfunctions under these superordinate two outcome variables. Of note, we did not include data of patients with deterioration of a pre-existing neurological dysfunction. Twenty-nine studies [10,18,19,23,24,28,29,31,33–35,37,38,40–43,48,49,51–55,57–59,61,62] reported new postoperative neurological dysfunctions after 565 (14.0%) of totally 4029 AC procedures. A later follow up result (six months) was provided for 279 of these patients with new neurological dysfunction. It showed a persistent neurological dysfunction in 64 patients. Of note, late neurological outcome after six months was reported in only seventeen studies comprising 2085 AC procedures in total. Considering twenty-six studies [10,18,19,23,24,28,29,34,35,37,38,40,41,43,48,49,51–55,57–59,61,62], which were reasonable included in our meta-analysis, the proportion of new neurological dysfunction was estimated to be 17% [95%CI: 12–23], with a high heterogeneity (I2 = 90%) (Fig 5). Meta-regression analysis did not reveal a difference depending on the anaesthesia technique (MAC/ SAS) (QM = 1.52, df = 1, p = 0.217), with an OR of 1.66 [95%CI: 1.35–3.70]. Furthermore, there is a large proportion of residual heterogeneity (QE = 187.55, df = 24, p < .0001), which cannot be explained by the applied anaesthesia technique. However, it has to be noted that there are only six studies available in the SAS group.
The summary value is an overall estimate from a random-effect model. The vertical dotted line shows an overall estimate of outcome proportion (based on the meta-analysis) disregarding grouping by technique. Neurol. dysf., neurological dysfunction.
Other adverse events/outcomes.
The other extracted adverse events and outcome data are shown in Tables 4 and 5. Mortality was very low with 10 patients (0.2%) of all forty-four studies comprising 5381 patients, which reported the outcome variable mortality (Table 5). Of note, two deaths include probably duplicate patients [42,43] to the study of Grossman et al. . Furthermore, we have only included deaths within 30 days after surgery in this analysis.
Interestingly 44% of the patients in the AAA approach of Hansen et al. experienced arterial hypertension , but this refers only to the test phase. During the pinning, craniotomy and tumour resection there were only 5 patients with 10–20% increase in blood pressure.
The analysis of the composite outcome, including AC failure, intraoperative seizure and mortality was based on forty-one studies (S1 Fig) [10,17–26,28–30,32,34–41,43,46–62]. Of note, intraoperative seizure events, which concurrently led to an AC failure, were counted only once for this composite outcome. The total proportion was estimated to be 8% [95% CI: 6–11], with 8% [95% CI: 6–12] in the MAC group and 8% [95% CI: 5–12] in the SAS group. Logistic meta-regression did not show a difference of the event rate depending on the technique (MAC/ SAS). The OR was 0.9 [95% CI: 0.47–1.76] and the residual heterogeneity I2 = 80%.
Sensitivity analysis, by including only prospectively conducted trials, was performed to look at the robustness of our findings in the main summary measure analyses of the four outcomes (AC failure, conversion to GA, intraoperative seizure and new neurological dysfunction) and the additional analysis of the composite outcome. Sensitivity analysis referred to eighteen trials [10,17,18,21,22,25,26,28,30,32,35,36,38,47,52,55,56,61], after exclusion of one duplicate study . Of note, it was not possible to predict an estimate for the outcome new neurological dysfunction in the SAS group, because only one prospective SAS study provided data for this outcome .
The proportions of outcomes were slightly lower in prospective studies compared to results from the main analysis, which is shown in S2 Fig. The logistic meta-regression models using the independent variables anaesthesia technique (MAC/ SAS) and prospective studies (yes/ no) showed only very small and statistically not significant differences.
Our systematic review has pointed out forty-seven studies addressing three main topics: SAS-, MAC- and AAA-technique of anaesthesia management for AC since 2007. We identified only two small RCTs [32,56] and one pseudo-RCT . These were as well as the remaining observational studies of moderate to low methodological quality. In summary all three anaesthetic approaches were feasible and safe. But our results have to be seen within their limits. Nine of the identified forty-seven studies reported partially duplicate patient data, first the studies of Ouyang et al. [45,46], second the studies from Tel Aviv [31,42,43], third the studies from Glostrup [20,44] and at least the studies of Boetto and Deras et al. [22,27]. Furthermore, the results from our meta-analysis are dominated by two larger retrospective studies with 611 , respectively 477 patients  and one prospective study with 511 patients . This was partially taken into account in our meta-analysis with the use of the random effects model, which applies less weight to large studies than fixed effect models. The meta-analyses revealed no statistically significant differences of AC failures, intraoperative seizures, new neurological dysfunctions, and the composite outcome (AC failure, intraoperative seizure, mortality) depending on the use of SAS or MAC technique. We found a substantial heterogeneity between the included studies and only the heterogeneity for conversion to GA showed a possible significant connection to the anaesthesia technique in the logistic meta-regression analysis. This analysis suggested significantly more unplanned conversions into GA with the use of SAS than MAC anaesthesia technique. However, this result was mainly depending on one high risk of bias retrospective SAS study with 6 events in 102 patients . Removing this study abolishes the significant difference between the techniques. Of note, two of the patients in this study required conversion into GA due to an air embolism, which was most likely related to the half-sitting patient position and not the used anaesthesia technique . Although air embolism was not analysed in detail in our SR, this was the only study, which reported a failure of AC due to this life-threatening adverse event. Furthermore, the sensitivity analysis, which included only prospective studies, confirmed the weakness of the result obtained by the main meta-regression analysis. A significant difference between the used anaesthesia techniques in regard to conversion to GA could not be revealed by the sensitivity analysis anymore. The decision to perform a sensitivity analysis by including only prospective studies and not the largest ones, was justified by the increased risk for confounding in larger studies due to a prolonged study duration. The most studies with more than 100 AC procedures, where performed during 5–8 [22,31,42,43,45,46,52] or 10–18 years [34,35,37,55,57]. The probability of a continuously same anaesthesia or AC surgery conduction in these observational studies during the large time-spans is very low. Our sensitivity analysis did also not reveal any statistical significant difference for the other four outcomes, which were included in the meta-analyses. Of note, the new neurological dysfunction outcome was only presented by one prospective study  in the SAS group. Therefore, we could not estimate the proportions for this outcome in the meta-analysis (S1 Fig). However, the main analysis included also only six studies in the SAS group [23,37,38,51,53,57] and the result was dominated by this prospective observational study of Li et al. in a Chinese population . Although 53.8% of the 91 patients exhibited new neurological dysfunctions, these dysfunctions remained permanent only in 1 patient, which suggests that the aim of safe resection was achieved in the longer-term. Furthermore, the generalizability of their results is unclear, due to possible differences in the distribution of the Chinese language areas compared to Non-Chinese people. Therefore we suggest interpreting our result of the meta-regression analysis in regard to new neurological dysfunctions with caution. According to previous investigations , Nossek et al. showed a better neurological outcome in the AC, than in the failure group . Furthermore, similar to other studies [65,66], Grossman et al.  could confirm a longer survival time depending on the extent of tumour resection. Of, note a selection bias in this analysis cannot be excluded, as there were most likely baseline differences between the patients who underwent AC with gross total resection and patients who underwent only biopsy or subtotal resection. Kim et al. underlined the importance of gross total resection particularly with regard to a significantly better neurological outcome .
Awake craniotomy is a demanding but safe procedure, none of the patients involved in the studies selected for this SR showed a serious adverse event, which could not be handled during AC. Well-considered patient selection has a big impact on the success of AC. More studies including multi-morbid or high-risk patients are required to confirm their eligibility to undergo AC as reported in four of our identified studies [28,34,43,45]. Pre-/ and postoperative MRI and neuropsychological testing were reported in almost all studies and should be performed routinely before AC. Additional recording of neurological exam videos before and after surgery may facilitate the neurological outcome measurement . Bilotta et al. described their experience with perioperative language testing by an anaesthesiologist in twenty patients undergoing MAC technique for AC . They pointed out the importance of perioperative language testing, also in settings without the presence of a professional language therapist. During the pre-operative language testing they identified patients with risk for postoperative language disturbances and patients with pre-operative deficits, which facilitated intraoperative identification of language deterioration. Furthermore, the patients were prepared for the upcoming intraoperative language testing tasks.
Administration of RSNBs, independent of the anaesthetic technique, has evolved as a safe and reasonable supportive procedure at the beginning of AC. This procedure appears to be superior over merely local scalp infiltration, as it blocks superficial as well as nociceptive afferents to profound tissues . A recent systematic review and meta-analysis of RCTs evaluated postoperative pain after RSNB for craniotomy . The published RCTs of RSNBs were small and of limited methodological quality, but the meta-analysis showed a consistent finding of reduced postoperative pain. Although RSNBs have potential complications, like local anaesthetic toxicity, hypertension, infection, haematoma, nerve injuries and inadvertent subarachnoid injection , this SR did not identify any adverse events associated with this procedure . In contrast, a case series of McNicholas et al., including 42 patients with RSNBs, reported seven patients with transient postoperative facial nerve palsy. They recommend limiting the local anaesthetic volume for auriculotemporal nerve block to 3 ml, and staying above the level of the tragus.  The specific learning rate to apply adequate RSNB is about ten procedures .
Postoperative questionnaires in the study of Beez et al.  revealed only in 5.1% severe discomfort, while the preoperative preparation was rated adequate in 94.9%. Other studies support these findings with postoperative satisfaction rates of 96.5% up to 100% [20,44,47,52,60]. Degree of satisfaction measured by visual analogue scale (VAS) in one study , which compared propofol-based to dexmedetomidine-based SAS protocol, showed a high degree of satisfaction (VAS 92) in both patient groups. In contrast, the blinded surgeons`satisfaction was significantly higher in the dexmedetomidine group. Careful patient-positioning is a further crucial factor influencing the success of AC, due to patient comfort and compliance . Active participation of the patients in the positioning phase supported probably the high patient satisfaction (84%) in a further study .
Avoidance of PONV is another contributing factor for patient satisfaction after AC. Beside this, PONV bears the risk of dehydration and in case of vomiting it could increase critically the intracranial pressure . Incidence of Nausea within 24h after craniotomy in GA technique was reported with 30–70% , favouring the use of antiemetic prophylaxis. Fabling et al. showed a significant reduction of PONV by prophylaxis with low dose droperidol or ondansetron in their RCT . Nausea was analysed intraoperatively in eleven of our included studies [17,18,20,27,30,36,44,51,54,56,59], and postoperatively in ten studies [17,18,30,33,45,46,50,51,54,58]. The intra- and postoperative incidences showed a range between 0 [18,30,46,51,59] and 30% [45,46]. The effect of antiemetic prophylaxis could not be evaluated for all of these studies, as it was not reported entirely. Ouyang et al. used ondansetron as well as dexamethasone and had a similar incidence of 30% as previously reported for patients receiving ondansetron . Interestingly, preoperative midline shift of averagely 5.96mm did not enhance the risk for PONV , although it is an independent risk factor for intraoperative brain oedema. The tumour histopathology was also not associated with an increased incidence of PONV .
Usefulness of BIS, or equal monitoring of anaesthesia depth, remains debatable in patients with neurological disorders, or antiepileptic drug therapy. While one report a strong delay in actual BIS values and awareness in AC patients with lower values than 80 , others recommend its use for AC . However, in our review there was no difference between the occurrence of AC failures in studies, which did not use any objective anaesthesia depth control [10,18–22,24,25,27–29,32,34–38,40–44,47,49–52,54,55,60,61] compared to studies, which used either RE or BIS monitoring [23,26,33,39,48,53,56,58,59,62]. Favourable evidence for using BIS in SAS was shown in one study, where the patients recovered faster if the BIS values were targeted to higher levels before commence of the awake phase . Another study with MAC anaesthesia showed significantly reduced propofol and fentanyl dosages in patients with BIS monitoring compared to patients without . This could have an impact on the success of awake surgery tasks. The influence of prior sedation on the cognitive and motoric ability to perform intraoperative tasks . Reduction of propofol dosage was also the aim in a further of our included studies . Interestingly, they used the volatile anaesthetic sevoflurane until the dura opening for this purpose. Due to the cerebral vasodilatative effect of sevoflurane, it is at higher risk for increasing intracranial pressure and brain swelling compared to intravenous agents, especially in patients with pre-existing intracranial hypertension . Abdou et al. used so-called "ketofol" anaesthesia, comprising ketamine and propofol mixture in one syringe, to avoid the side effects of opioids and reduce the propofol requirement . The generalizability of this method remains questionable, as this mixture is not approved in many countries, like e.g. Germany. Clear evidence exists for meticulous preparation to handle intraoperative seizures. Most seizures occur in regard to cortical stimulation, which should be discontinued immediately and direct cortex irrigation with cold saline solution should take place . This method was used throughout all AC studies, which we have analysed in this review. Only resistant seizures were treated escalating with small doses of benzodiazepines, propofol or thiopental, antiepileptic drugs, or GA. Furthermore, it is beneficial to recognise already preoperatively patients at higher risk for intraoperative seizures. Patients with tumours in the frontal lobe , and especially the supplementary motor area  showed a higher incidence of intraoperative seizures and this should be considered during the patient preparation. Furthermore, younger patients, patients with low-grade glioma and history of seizures were prone for intraoperative seizures [29,31,37,43]. Adequate treatment with antiepileptic drugs (AEDs) could not prevent the occurrence of intraoperative seizures [29,42], similar to previous findings . Moreover caution is required for patients receiving phenytoin perioperatively, as it probably increases the risk for communication failures during AC . Length of hospital stay was rarely described in the included studies (Table 5) and is very difficult to compare between different healthcare systems, thus a reasonable meta-analysis was not feasible. Interestingly, one study showed a substantial longer length of stay (13.3±4.2 days) than the others , which is probably explained by their hospital policy. In contrast, there is some evidence that AC can also be performed as a same day surgery procedure . AC failure rate was our primary outcome of interest, as it plays a crucial role in the extent of the tumour resection, the consecutive postoperative survival time and neurological outcome of the patients . Shinoura et al. could confirm a significantly impaired neurological function after failed AC . Sacko et al. additionally pointed out the importance of successful awake surgery for tumours near eloquent brain areas . They found a significantly better neurological outcome, higher proportion of GTR and shorter hospital length of stay in patients undergoing AC compared to GA. Ali et al. had similar results, favouring AC . In contrast, Gupta et al. could not find any significant differences between GA and AC in their RCT, except for the procedure time, which was significantly shorter in the GA group . The total reported failure rate for all three AC techniques (excluding the partially duplicate studies [27,42,44] was 1.7% (68 out of 4063 patients). This was confirmed by our meta-analysis of the MAC and SAS studies (Fig 2). Due to the heterogeneity and low quality of all included studies, this result has also to be seen within its limits. Only a large-scale multi-centre randomised controlled trial, with a standardised perioperative protocol would enable a definitive distinction of these two procedures. Furthermore, the AAA technique , with its low failure rate of 1 out of 50 patients seems to have potential for implementation in AC, but further clinical data are required to confirm the feasibility of this technique to larger populations.
Dexmedetomidine has been successfully used for MAC as well as the SAS technique in our included studies. Further investigations are required to show a potential significant superiority of dexmedetomidine to especially propofol.
First, we accessed only two databases and restricted our search to English language, which might not have identified all clinical studies meeting our inclusion criteria. Second, we focused our search on the years 2007–2015, to analyse the recent development of anaesthesia techniques for AC. One justification for our chosen time-span is the continuous development of the anaesthetics and our aim to provide a SR for the actually usually used anaesthetics. Another one is that the information quality of clinical articles is significantly depending on the reporting quality. As the most of the identified studies were of observational nature, we have decided to include only studies since 2007, when the latest STROBE statement for improving reporting quality of observational trials was published . Of note, some of our included studies were already published in 2007 before the latest STROBE statement release. Due to our specific search strategy we identified only forty-seven studies, which were mostly observational, retrospective and heterogeneous. All studies, including the two small RCTs with 26  and 30 patients , and one pseudo-RCT with 29 patients  had low methodological quality with a moderate to high risk of bias. Furthermore, the primary endpoint of the pseudo-RCT, which used an alternating assignation method, was the difference between listening to major key or minor key music during AC , which was not our focus in this SR. Eleven studies were performed during a large time-scale of more than six years [24,31,35,37,38,42,43,47,55,57,59], and even 18 years . It is likely, that the findings were strongly affected by a learning curve of the whole team involved in conduction of AC. This implies also the anaesthetic techniques, which were subjects to change. Of note, outcome assessment differed significantly between the studies. Our inclusion of small studies ≤20 patients [19,23,28,39,54,60], bears the risk of overestimation of beneficial outcomes, due to random chance . Furthermore, the estimated treatment-effect tends to be larger in non-randomised studies . Our pre-described outcome variables were not reported in each of the identified studies and hindered therefore a meta-analysis of more than five outcome variables. Inclusion of observational studies into our meta-analysis was justified by the absence of better evidence for the different anaesthetic AC techniques, presently. Concurrently, a sensitivity analysis could only be performed by inclusion of these observational studies, despite the present high-risk of bias in them. Furthermore, we have excluded studies, which were performed outside the operating room or with the use of an intraoperative MRI guidance. This decision was made due to the limited generalizability of these techniques to many hospitals, which do not have a complex infrastructure and the potentially prolonged surgery time by using them. In addition, it has to be acknowledged that the neurological outcome measures and the detection of intraoperative seizures may have differed between the studies.
SAS and MAC technique for AC seem to be similarly safe without serious complications, whereas evidence for the AAA technique is limited. AC requires a multidisciplinary teamwork and personal experience. The anaesthesiologist has to be skilled in multiple areas, including local anaesthesia for RSNB, advanced airway management, dedicated sedation protocols, an exquisite management of haemodynamics and a high rapid alert to treat possible intraoperative adverse events. AC can be conducted safely even in patients older than 65 years. The neurological outcome can be preserved and even improved in patients undergoing AC. A consequently performed local anaesthesia and scalp nerve block reduces the requirement of sedative agents and postoperative pain. The additionally use of dexmedetomidine enables further reduction of opioid and propofol infusion, while preserving haemodynamic stability. The benefit of MAC and AAA technique consists of reduction/ waiving of sedatives, which probably improves the intraoperative brain mapping. Large RCTs with a standardised protocol are required to prove if there is a significant superiority of one of the three anaesthetic regimes for AC.
S1 Fig. Forrest plot of the composite outcome.
The summary value is an overall estimate from a random-effect model. The vertical dotted line shows an overall estimate of outcome proportion (based on the meta-analysis) disregarding grouping by technique. Of note, Souter et al.  have used both anaesthesia techniques. The composite outcome comprised the outcomes: awake craniotomy failure, intraoperative seizures and mortality within 30 days of surgery.
S2 Fig. Comparison between all and prospective studies.
The figure shows the predicted proportions for each outcome. The left panels depict results for all studies, and right panels show results for prospective studies only. Of note there is no estimate for new neurological dysfunctions in the SAS group among prospective studies, because only one study provided data.
S2 File. Results of general considerations for AC.
S1 Table. Patient characteristics.
HGG, high grade glioma; LGG, low grade glioma; NK, not known; SD, standard deviation.
S2 Table. Risk of bias assessed with the Cochrane Collaboration’s risk of bias tool. +, high risk; -, low risk;?, unknown risk
AC, awake craniotomy; BIS, bispectral index; CT, computed tomography; MMSE, mini-mental state examination; MRI, magnetic resonance imaging; PONV, postoperative nausea and vomiting; VAS, visual analogue scale.
We would like to thank Dr. Andras Keszei (Department of Medical Informatics, University Hospital RWTH Aachen, Germany) for his excellent support with the statistical analysis.
Conceived and designed the experiments: AS RR MV FB MC. Performed the experiments: AS MC. Analyzed the data: AS RR MV FB MC. Contributed reagents/materials/analysis tools: AS RR MV FB MC. Wrote the paper: AS RR MV FB MC.
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