Bactrocera latifrons is a serious pest of solanaceous fruits and Bactrocera umbrosa is a pest of Artocarpus fruits, while Bactrocera melastomatos infests the fruit of Melastomataceae. They are members of the subgenus Bactrocera. We report here the complete mitochondrial genome of these fruit flies determined by next-generation sequencing and their phylogeny with other taxa of the subgenus Bactrocera. The whole mitogenomes of these three species possessed 37 genes namely, 13 protein-coding genes (PCGs), 2 rRNA and 22 tRNA genes. The mitogenome of B. latifrons (15,977 bp) was longer than those of B. melastomatos (15,954 bp) and B. umbrosa (15,898 bp). This difference can be attributed to the size of the intergenic spacers (283 bp in B. latifrons, 261 bp in B. melastomatos, and 211 bp in B. umbrosa). Most of the PCGs in the three species have an identical start codon, except for atp8 (adenosine triphosphate synthase protein 8), which had an ATG instead of GTG in B. umbrosa, whilst the nad3 (NADH dehydrogenase subunit 3) and nad6 (NADH dehydrogenase subunit 6) genes were characterized by an ATC instead of ATT in B. melastomatos. The three species had identical stop codon for the respective PCGs. In B. latifrons and B. melastomatos, the TΨC (thymidine-pseudouridine-cytidine)-loop was absent in trnF (phenylalanine) and DHU (dihydrouracil)-loop was absent in trnS1 (serine S1). In B. umbrosa, trnN (asparagine), trnC (cysteine) and trnF lacked the TψC-loop, while trnS1 lacked the DHU-stem. Molecular phylogeny based on 13 PCGs was in general concordant with 15 mitochondrial genes (13 PCGs and 2 rRNA genes), with B. latifrons and B. umbrosa forming a sister group basal to the other species of the subgenus Bactrocera which was monophyletic. The whole mitogenomes will serve as a useful dataset for studying the genetics, systematics and phylogenetic relationships of the many species of Bactrocera genus in particular, and tephritid fruit flies in general.
Citation: Yong H-S, Song S-L, Lim P-E, Eamsobhana P, Suana IW (2016) Complete Mitochondrial Genome of Three Bactrocera Fruit Flies of Subgenus Bactrocera (Diptera: Tephritidae) and Their Phylogenetic Implications. PLoS ONE 11(2): e0148201. https://doi.org/10.1371/journal.pone.0148201
Editor: Bi-Song Yue, Sichuan University, CHINA
Received: October 13, 2015; Accepted: January 14, 2016; Published: February 3, 2016
Copyright: © 2016 Yong et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: The whole mitochondrial genome sequences are available in GenBank database (accession number: Bactrocera latifrons KT881556; Bactrocera melastomatos KT881557; and Bactrocera umbrosa KT881558).
Funding: This study was funded in part by Ministry of Higher Education High Impact Research Grant (H-50001-00-A000025) and the University of Malaya (H-5620009).
Competing interests: The authors have declared that no competing interests exist.
Fruit flies in the genus Bactrocera are potentially destructive pests of commercial fruits and vegetables . Seventy-three species have been documented as economically important in the Pacific Region . Seven out of the nine species are rated as the most serious pests (Category A) , these being members of the subgenus Bactrocera; the other two less harmful species are B. (Daculus) oleae (Gmelin) and B. (Zeugodacus) cucurbitae (Coquillett). B. latifrons (Hendel) is one of the seven pest species belonging to the subgenus Bactrocera; the other members are B. (B.) carambolae Drew and Hancock, B. (B.) correcta (Bezzi), B. (B.) dorsalis (Hendel), B. (B.) neohumeralis (Hardy), B. (B.) tryoni (Froggatt), and B. (B.) zonata (Saunders). Bactrocera latifrons fruit hosts are mainly Solanaceae and Cucurbitaceae although 59 plant species from 14 plant families have been documented . This pest has a broad geographical range occurring in Pakistan, India, Sri Lanka, Myanmar, China, Taiwan, Thailand, Laos, Vietnam, Malaysia, Singapore, Brunei, and Indonesia, and has been introduced into Hawaii, Okinawa, Tanzania, and Kenya [2–6].
A less serious pest species of the subgenus Bactrocera is B. umbrosa (Fabricius)–one of 16 species in Category C consisting of relatively minor oligophagous or specialist fruit or cucurbit pests . It infests Artocarpus fruits and is widespread from southern Thailand through New Guinea to New Caledonia . Another species, B. melastomatos Drew & Hancock of the subgenus Bactrocera is not known to damage commercial crop plants but infests the fruit of Melastomataceae . It has been documented in India (Andaman Island), Thailand, Peninsular Malaysia, Singapore, and Indonesia (Java, Kalimantan, Sumatra) .
There are few reports on the molecular phylogeny of B. latifrons, B. melastomatos and B. umbrosa. Based on 16S rRNA and cytochrome oxidase I nucleotide sequences, B. latifrons shows close affinity to B. umbrosa and is most basal to subgenus Bactrocera . In another study based on cytochorome oxidase I, B. umbrosa forms a sister group with B. facialis while B. latifrons is basal to subgenus Bactrocera . Based on 17 enzyme loci profile using starch-gel electrophoresis, B. melastomatos is distinct from the lineage of B. dorsalis and B. carambolae .
To date, the complete mitochondrial genomes (mitogenomes) of six species of the subgenus Bactrocera–B. arecae, B. carambolae, B. correcta, B. dorsalis (incuding the conspecific taxa B. papayae and B. philippinensis), B. tryoni, and B. zonata–are available in GenBank. We report here the mitogenome of three additional species of the subgenus (B. latifrons, B. melastomatos, and B. umbrosa) determined by next-generation sequencing and their phylogenetic relationships with other taxa of the subgenus Bactrocera.
Materials and Methods
B. latifrons, B. melastomatos and B. umbrosa are insect pests. They are not endangered or protected by law. No permits are required to study these fruit flies.
Fruit flies of B. latifrons were hatched from infested chilli fruit (Capsicum annuum) collected in University of Malaya campus . Male fruit flies of B. melastomatos were collected by means of Cue lure  and B. umbrosa by means of methyl eugenol in University of Malaya campus. The specimens were preserved in 95% absolute ethanol and stored in -20°C freezer until use.
Mitochondria isolation and DNA extraction
A small piece of the alcohol-preserved tissue of each Bactrocera species was pressed onto a C-fold paper towel to remove excess ethanol before homogenisation. The mitochondria were isolated by standard differential centrifugation method  and the mtDNA was extracted using Mitochondrial DNA Isolation Kit (Abnova, Taipei, Taiwan) following the manufacturer’s instructions with minor modification. The mtDNA was eluted using 30 ul elution buffer instead of Tris-EDTA (TE) buffer to avoid interference of Ethylenediaminetetraacetic acid (EDTA) with the enzyme such as transposases.
Sample and library preparation
The purified mtDNA was quantified using Qubit dsDNA High Sensitivity Assay Kit (Life Technologies, USA) and normalized to a final concentration of 50 ng (20 ul of mtDNA at 2.5 ng/ul). Library was prepared using Nextera DNA Sample Preparation Kit (Illumina, USA) following the manufacturer’s protocols. Size estimation of the library was performed on a 2100 Bioanalyzer using High Sensitivity DNA Analysis Kit (Agilent Technologies). The library was quantified with Qubit 2.0 Fluorometer (Life Technologies, USA).
The library was normalized to 12 picomolar and sequenced using the NextSeq 500 Dekstop Sequencer (2 × 150 bp paired-end reads) (Illumina, USA).
Sequence and genome analysis
Raw sequence reads were extracted from the Illumina NextSeq 500 system in FASTQ format. The quality of sequences was evaluated using the FastQC software . All ambiguous nucleotides and reads with an average quality value lower than Q20 were excluded from further analysis. The trimmed sequences were mapped against three reference mitogenomes namely, Bactrocera dorsalis (NC_008748), B. tryoni (NC_014611) and B. zonata (NC_027725) using the CLC Genomic Workbench version 8.0.1 (Qiagen, Germany) with mapping parameters of length fraction = 0.6 and similarity fraction = 0.7. The mapped sequences were then subjected to de novo assembly. Contigs greater than 15 kbp were subjected to BLAST  alignment against the nucleotide database at National Center for Biotechnology Information (NCBI). Contigs with hits to mitochondrial genes or genomes were identified and extracted using the CLC Genomic Workbench interface.
Mitogenome identification, annotation and visualization
A single contig which blasted as mitochondrial sequence was manually examined for repeats at the beginning and end of the sequence to establish a circular mtDNA. It was then annotated with MITOS  followed by manual validation of the coding regions. Open reading frames (ORFs) were predicted using the NCBI ORF Finder (http://www.ncbi.nlm.nih.gov/gorf/gorf.html). The sequin file generated from MITOS was edited and submitted to NCBI according to ORF Finder result and can be accessed at NCBI GenBank using the accession numbers: Bactrocera latifrons KT881556; Bactrocera melastomatos KT881557; and Bactrocera umbrosa KT881558. The circular mitogenome was visualized with Blast Ring Image Generator (BRIG) .
Mitogenomes from GenBank
The mitogenomes of Tephritidae available from GenBank (Bactrocera dorsalis NC_008748, NC_009790, NC_009771; B. carambolae NC_009772; B. arecae KR233259; B. correcta NC_018787; B. tryoni NC_014611; B. zonata NC_027725; B. oleae NC_005333; B. minax NC_014402; B. cucurbitae NC_016056; B. scutellata NC_027254; B. tau NC_027290; B. caudata Malaysia KT625491; B. caudata Indonesia KT625492; Ceratitis capitata NC_000857; Procecidochares utilis NC_020463) were used for phylogenetic comparison. Species of Drosophila (D. incompta NC_025936; D. melanogaster NC_024511; D. yakuba NC_001322) were used as outgroup taxa.
The total length of the aligned sequences of each mitogenome comprised of 13 protein-coding genes (PCGs), 2 rRNA genes and 15 mt-genes (13 PCGs, 2 rRNA genes). This data as well as the selected models used for maximum likelihood (ML) and Bayesian Inference (BI) analyses are summarized in Table 1.
AIC, Akaike Information Criterion; BIC, Bayesian Information Criterion.
The 13 PCG sequences were separately aligned using ClustalX v.1.81 program  and were subsequently edited and trimmed using BioEdit v.184.108.40.206 . The sequences of the large-(rrnL) and small-(rrnS) subunit genes were aligned using MAFFT v.7  (The aligned sequences can be given upon request). Kakusan v.3  was used to determine the best-fit nucleotide substitution models for maximum likelihood (ML) and Bayesian (BI) analyses based on the corrected Akaike Information Criterion  and the Bayesian Information Criterion , respectively.
Phylograms of 13 concatenated PCGs, 2 rRNA genes and 15 mt-genes were constructed using TreeFinder . Bootstrap values (BP) were generated via 1,000 ML bootstrap replicates. Bayesian analyses were conducted using the Markov chain Monte Carlo (MCMC) method via Mr. Bayes v.3.1.2 , with two independent runs of 2×106 generations with four chains, and with trees sampled every 200th generation. Likelihood values for all post-analysis trees and parameters were evaluated for convergence along with burn-in (a specified number of samples from the beginning of the chain to be discarded) using the “sump” command in MrBayes and the computer program Tracer v.1.5 (http://tree.bio.ed.ac.uk/software/tracer/). The first 200 trees from each run were discarded as burn-in (where the likelihood values were stabilized prior to the burn-in), and the remaining trees were used for the construction of a 50% majority-rule consensus tree. Phylogenetic trees were viewed and edited by FigTree v.1.4 . To assess the level of variation, uncorrected pairwise (p) genetic distances were estimated using PAUP* 4.0b10 software .
Mitogenome analysis and features
The sequencing reads, GC content and base composition of Bactrocera mitogenomes produced by next-generation sequencing on Illumina NextSeq 500 Desktop Sequencer are summarized in Table 2.
The mitogenomes of B. latifrons, B. melastomatos and B. umbrosa had similar gene order and contained 37 genes (13 protein-coding genes—PCGs, 2 rRNA genes, and 22 tRNA genes) and a non-coding region (A + T-rich control region) (Fig 1, S1–S3 Tables). Control region was flanked by rrnS and trnI genes respectively, with 953 bp in B. latifrons and B. melastomatos, and 944 bp in B. umbrosa. A long polyT-stretch of 23 bp in B. latifrons, 20 bp in B. melastomatos, and 24 bp in B. umbrosa was observed.
GC skew is shown on the outer surface of the ring whereas GC content is shown on the inner surface. The anticodon of each tRNAs is shown in bracket.
There were 15 intergenic regions with spacing sequence totalling 283 bp in B. latifrons, 261 bp in B. melastomatos, and 211 bp in B. umbrosa. The region between trnQ and trnM genes was separated by 94 bp in B. latifrons, 82 bp in B. melastomatos, and 79 bp in B. umbrosa. Sequences with 39, 43 and 94 bases in B. latifrons, 35, 39 and 82 bases in B. melastomatos, and 79 bases in B. umbrosa had clear stem-loop structures. All the three species had overlaps in seven regions totalling 29 bp.
The three species shared an identical start codon for most of the PCGs, except ATG (instead of GTG) for atp8 in B. umbrosa, and ATC (instead of ATT) for nad3 and nad6 in B. melastomatos (S4 Table). Of the start codons common to the three species, the commonest was ATG (in 6 PCGs–cox2, atp6, cox3, nad4, nad4l, cob), followed by two ATT (nad2, nad5) and one each for ATA (nad1) and TCG (cox1). The three species had an identical stop codon for the respective PCGs (S4 Table). Seven PCGs has a TAA stop codon (nad2, cox2, atp8, atp6, cox3, nad4l, nad6), one had TAG (nad4), and five had truncated stop codon (1 TA–cox1; 4 T–nad3, nad5, cob, nad1).
The nucleotide compositions of the mitochondrial whole genome, protein-coding genes, rRNA genes and control region of B. latifrons, B. melastomatos and B. umbrosa are summarized in S5–S7 Tables. All three species were A+T rich as expected for mitochondrial genomes. The A + T content for PCGs was lowest in cox1 (61.8% for B. latifrons, 65.0% for B. melastomatos, and 60.5% for B. umbrosa) and highest in nad4l (76.4% for B. latifrons and 74.4% for B. umbrosa) and nad6 (78.7% for B. melastomatos). The A + T content of the non-coding control region was 86.8% for B. latifrons, 89.0% for B. melastomatos and 86.2% for B. umbrosa. For the two ribosomal operons, rrnL had a higher A + T content than rrnS (78.9% vs 74.4% for B. latifrons, 80.2% vs 74.6% for B. melastomatos, and 79.0% vs 73.6% for B. umbrosa). The GC skew content which included the whole genome, PCGs, rRNA genes and control region in the three species were negative indicating a bias toward the use of Cs over Gs. Although the AT skewness value was positive for the whole genome, rRNA genes and control region, it was variable in the individual PCGs.
As in other insects, the mitogenomes of B. latifrons, B. melastomatos and B. umbrosa had three main tRNA clusters which are characteristically depicted in Fig 1. These include: (1) I-Q-M; (2) W-C-Y; and (3) A-R-N-S1-E-F (Fig 1). The cloverleaf structure for the respective tRNAs was similar in B. latifrons and B. melastomatos. The TΨC-loop was absent in trnF while trnS1 lacked the DHU-loop (S1 and S2 Figs). In B. umbrosa, trnN, trnC and trnF lacked the TψC-loop, while trnS1 lacked DHU-stem (S3 Fig).
Phylogenetic relationships and genetic divergence
The molecular phylogeny of B. latifrons, B. melastomatos and B. umbrosa in relation to other Bactrocera taxa of the subgenus Bactrocera and other Tephritidae are shown in Fig 2. The phylogram based on 13 PCGs was in general congruent with that based on 15 mt-genes, except for the position of B. melastomatos. The subgenus Bactrocera was monophyletic, forming a distinct clade from the other Bactrocera taxa in which the subgenus Zeugodacus was monophyletic. Of the three species in the present study, B. latifrons and B. umbrosa formed a sister group and were basal to the other taxa of the subgenus Bactrocera.
Numeric values at the nodes are Bayesian posterior probabilities/ML bootstrap. Figures 3 and 4 in Yong et al. (2015)  were interposed.
The genetic diversity of B. latifrons, B. melastomatos, B. umbrosa and related taxa of the subgenus Bactrocera based on (a) 13 PCGs, (b) 2 rRNA genes, and (c) 13 PCGs + 2 rRNAs genes is summarized in Table 3.
Mitochondrial genomes of insects are extensively studied with particular reference to their phylogenetic and evolutionary studies . The use of heterogeneous CAT and CAT 1 GTR models indicates that the complete nucleotide sequences (PCG and PCGRNA) of mitogenome are suitable for resolving higher-level phylogeny of Paraneopteran insects . To date there are complete mitogenomes for six species of the subgenus Bactrocera namely, B. dorsalis, B. carambolae, B. arecae, B. correcta, B. tryoni, and B. zonata. The present study has added three more species to this list.
The mitogenome size of B. umbrosa (15,898 bp) is smaller than those of B. latifrons (15,977 bp) and B. melastomatos (15,954 bp) (S1–S3 Tables). This is due mainly to the size of the intergenic spacers– 211 bp in B. umbrosa, 261 bp in B. melastomatos and 283 bp in B. latifrons. Among the mitogenomes of the subgenus Bactrocera available in GenBank, B. dorsalis (including the conspecific B. papayae and B. philippinensis) and B. carambolae have a mitogenome size of 15,915 bp, B. tryoni 15,925 bp, B. zonata 15,935 bp, and B. correcta 15,936 bp respectively.
The start and stop codons for the respective PCGs in the nine Bactrocera taxa of the subgenus are not invariant (S4 Table). They are identical in seven PCGs–nad2, cox1, cox2, cox3, nad4, nad4l, and nad1 (S4 Table). In this study, B. umbrosa differs from the other species in the possession of ATG (instead of GTG) start codon for atp8. B. melastomatos differs from the other species in having ATC (instead of ATT) start codon for nad3 and nad6.
Seven PCGs (cox1, atp6, nad3, nad5, nad6, cob, nad1) have incomplete stop codons in some members of the nine Bactrocera taxa of the subgenus Bactrocera (S4 Table); only TA for cox1 and T for nad1 are present in all the nine taxa. The incomplete stop codons (T and TA) can be converted to TAA by post-translational polyadenylation .
Among the tRNAs, trnF lacks the TΨC-loop in all the nine Bactracera taxa of the subgenus Bactrocera (Table 4). Two other tRNAs also lack the TΨC-loop–trnN in B. umbrosa, B. arecae, B. dorsalis, B. carambolae and B. tryoni; and trnC in B. umbrosa, B. dorsalis, B. carambolae and B. tryoni. trnS1 has aberrant cloverleaf structure for DHU arm, lacking DHU-stem in B. umbrosa and DHU-loop in eight of the nine taxa of the subgenus Bactrocera (Table 4). Deviant tRNA secondary structures are particularly frequent in Arthropoda . The TΨC-loop and DHU-loop of tRNA act as special recognition site during protein biosynthesis or translation [32–34]. It has been reported that misacylation of tRNA can affect the survivability of an organism .
Studies on molecular phylogeny of Bactrocera fruit flies have been based mainly on mitochondrial and nuclear genes, e.g. the phylogenetic relationships among (1) 24 Bactrocera species based on rrnL, cox2, trnK and trnD genes , (2) 125 Dacini species based on rrnL, cox1, cox2 and “white-eye” genes , (3) 47 Bactrocera species based on cox1 gene sequences , and (4) 56 Bactrocera taxa using cox1 and rrnL gene fragments .
Molecular studies have revealed considerable variation in genetic diversity among closely related taxa of Bactrocera fruit flies. A recent study based on six loci (cox1, nad4-3′, CAD, period, ITS1, ITS2) indicates that B. dorsalis s.s., B. papayae and B. philippinensis are the same biological species . Another taxon B. invadens has also been synonymized with B. dorsalis . Based on analysis of 13 PCGs, the uncorrected genetic ‘p’ distance is 1.06 between B. dorsalis and B. dorsalis (= papayae) and 1.11 between B. dorsalis and B. dorsalis (= philippinensis) . Analyses of the cox1, cox2, rrnL and concatenated cox1+cox2+rrnL and cox1+cox2+rrnL+28S+ITS-2 nucleotide sequences reveal that B. caudata from the northern hemisphere (Peninsular Malaysia, East Malaysia, Thailand) and southern hemisphere (Indonesia: Java, Bali and Lombok) are genetically distinct, with uncorrected ‘p’ distance of 4.46–4.94% for the concatenated cox1+cox2+rrnL nucleotide sequences which is several folds higher than the ‘p’ distance for the taxa in the northern hemisphere (‘p’ = 0.00–0.77%) and the southern hemisphere (‘p’ = 0.00%) .
Two recent studies on the mitogenomes of Bactrocera fruit flies of the subgenus Bactrocera have reported the sister lineage of B. correcta and B. zonata  and that of B. arecae and B. tryoni , in addition to the sister lineage of B. dorsalis and B. carambolae. The present results of B. melastomatos being distinct from the lineage of B. dorsalis and B. carambolae agree with earlier finding based on 17 enzyme loci profile using starch-gel electrophoresis .
In the present study, the subgenus Bactrocera is monophyletic (Fig 2). Of the other subgenera, B. (Daculus) oleae and B. (Tetradacus) minax form a clade with subgenus Bactrocera, while the subgenus Zeugodacus forms a distinct clade (Fig 2).
Based on 13 PCGs and 15 mt-genes, B. latifrons and B. umbrosa form a sister group basal to the other members of the subgenus Bactrocera (Fig 2). This finding concurs with that based on rrnL and cox1 sequences . However, it differs from that based on cox1 gene which reveals B. latifrons is most basal to the subgenus Bactrocera but does not form a lineage with B. umbrosa . The species tree differs from the finding based on cox1, rrnL, trnP, nad6 and period genes in which B. latifrons and B. umbrosa do not form a sister lineage . With the inclusion of B. latifrons, the present finding helps to resolve the inference of B. umbrosa (based on cox1, cox2, rrnS and rrnL nucleotide sequences) forming a lineage with B. (Gymnodacus) calophylli instead of with the subgenus Bactrocera . It is evident that a broader taxon sampling and the use of mitogenomes will enable a better understanding of the phylogeny of Bactrocera and other tephritid fruit flies.
In summary, we have successfully sequenced the whole mitochondrial genomes of B. latifrons, B. melastomatos and B. umbrosa by using next generation sequencing technologies. The mitochondrial genome features are similar to other tephritid fruit flies. The phylogenetic species tree based on 13 PCGs is in general concordant with that based on 15 mt-genes. Based on concatenated 13 protein-coding genes and 15 mt-genes of the mitogenome, B. latifrons and B. umbrosa form a sister lineage most basal to the subgenus Bactrocera. The subgenus Bactrocera is monophyletic. The whole mitogenomes will serve as a useful dataset for studying the genetics, systematics and phylogenetic relationships of the many species of Bactrocera genus in particular, and tephritid fruit flies in general.
S1 Fig. Cloverleaf structure of the 22 inferred tRNAs in the mitogenome of Bactrocera latifrons.
The cloverleaf structure for trnF lacked the TψC-loop, and trnS1 lacked the DHU-loop.
S2 Fig. Cloverleaf structure of the 22 inferred tRNAs in the mitogenome of Bactrocera melastomatos.
The cloverleaf structure for trnF lacked the TψC-loop, and trnS1 lacked the DHU-loop.
S3 Fig. Cloverleaf structure of the 22 inferred tRNAs in the mitogenome of Bactrocera umbrosa.
The cloverleaf structure for trnC and trnF lacked the TψC-loop, and trnS1 lacked the DHU-stem.
S1 Table. Characteristics of the mitochondrial genome of Bactrocera latifrons.
The anticodon of each tRNAs is shown in bracket. J (+) or N (-) indicates gene directions.
S2 Table. Characteristics of the mitochondrial genome of Bactrocera melastomatos.
The anticodon of each tRNAs is shown in bracket. J (+) or N (-) indicates gene directions.
S3 Table. Characteristics of the mitochondrial genome of Bactrocera umbrosa.
The anticodon of each tRNAs is shown in bracket. J (+) or N (-) indicates gene directions.
S4 Table. Start/stop codon of protein-coding genes (PCGs) of Bactrocera taxa of the subgenus Bactrocera.
Highlighted text indicates difference in start/stop codon with reference to B. latifrons.
S5 Table. Nucleotide composition of whole mitogenome, protein-coding genes, rRNA genes and control region of Bactrocera latifrons.
S6 Table. Nucleotide composition of whole mitogenome, protein-coding genes, rRNA genes and control region of Bactrocera melastomatos.
We thank our institutions for providing various research facilities and other support. Much of the text in the Methods sections (Sample and Library Preparation; Sequence and Genome analysis; Mitogenome Identification, Annotation, and Visualization; and Phylogenetic Analysis) has been reused from the previous PLoS ONE article (http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0134581).
Conceived and designed the experiments: H-SY S-LS. Performed the experiments: S-LS H-SY. Analyzed the data: S-LS H-SY P-EL. Contributed reagents/materials/analysis tools: H-SY P-EL S-LS PE IWS. Wrote the paper: H-SY S-LS P-EL.
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